LOCUS       NZ_KU852461            11069 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium strain
            ST1004 plasmid pNUC, complete sequence.
ACCESSION   NZ_KU852461
VERSION     NZ_KU852461.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227236
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Oliva,M., Monno,R., D'Addabbo,P., Pesole,G., Dionisi,A.M.,
            Scrascia,M., Chiara,M., Horner,D.S., Manzari,C., Luzzi,I., Calia,C.,
            D'Erchia,A.M. and Pazzani,C.
  TITLE     A novel group of IncQ1 plasmids conferring multidrug resistance
  JOURNAL   Plasmid 89, 22-26 (2017)
   PUBMED   27916622
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Oliva,M., Monno,R., Pietro,D., Pesole,G., Dionisi,A.M., Scrascia,M.,
            Chiara,M., Horner,D.S., Manzari,C., Calia,C., D'Erchia,A.M. and
            Pazzani,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2016) Department of Biology, University of Bari,
            via Orabona 4, Bary 70125, Italy
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 03:02:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 14
            CDSs (with protein)               :: 14
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KU852461.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /strain="ST1004"
                     /serovar="Typhimurium"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="pNUC"
                     /geo_loc_name="Italy"
     gene            join(10903..11069,1..76)
                     /locus_tag="HTG86_RS00005"
     CDS             join(10903..11069,1..76)
                     /locus_tag="HTG86_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(108..758)
                     /gene="tetR(A)"
                     /locus_tag="HTG86_RS00010"
     CDS             complement(108..758)
                     /gene="tetR(A)"
                     /locus_tag="HTG86_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            864..2063
                     /gene="tet(A)"
                     /locus_tag="HTG86_RS00015"
     ARG             865..2060
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1994..2434)
                     /locus_tag="HTG86_RS00020"
     CDS             complement(1994..2434)
                     /locus_tag="HTG86_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_025732385.1"
                     /translation="MSSNGRHFGEIRLSALKKAPEMFGSVYEHEEKKPMEAFAERLRDA
                     VAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVAGMLLRAVIDYGRE
                     HVEQIMLTVAEGNEAAINLYQRSARCPAPLQSPRRSAGRQSK"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(2893..3177)
                     /locus_tag="HTG86_RS00025"
     CDS             complement(2893..3177)
                     /locus_tag="HTG86_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            3481..>3969
                     /locus_tag="HTG86_RS00070"
                     /pseudo=""
     CDS             3481..>3969
                     /locus_tag="HTG86_RS00070"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            4124..4537
                     /locus_tag="HTG86_RS00075"
     CDS             4124..4537
                     /locus_tag="HTG86_RS00075"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            4636..5505
                     /locus_tag="HTG86_RS00080"
     CDS             4636..5505
                     /locus_tag="HTG86_RS00080"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            5566..5778
                     /locus_tag="HTG86_RS00035"
     CDS             5566..5778
                     /locus_tag="HTG86_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            5780..5986
                     /gene="urfF"
                     /locus_tag="HTG86_RS00040"
     CDS             5780..5986
                     /gene="urfF"
                     /locus_tag="HTG86_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            6016..6855
                     /locus_tag="HTG86_RS00045"
     CDS             6016..6855
                     /locus_tag="HTG86_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            6842..7693
                     /gene="repC"
                     /locus_tag="HTG86_RS00050"
     CDS             6842..7693
                     /gene="repC"
                     /locus_tag="HTG86_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            8001..8816
                     /gene="sul2"
                     /locus_tag="HTG86_RS00055"
     ARG             8002..8813
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9262..9680
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             8878..9677
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9680..10516
                     /locus_tag="HTG86_RS00065"
     CDS             9680..10516
                     /locus_tag="HTG86_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             9681..10513
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3132..3287
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          6034..6855
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          3377..5502
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
       61 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
      121 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
      181 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
      241 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
      301 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
      361 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
      421 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
      481 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
      541 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
      601 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
      661 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
      721 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
      781 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
      841 ggaggctggt ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca
      901 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
      961 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
     1021 cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
     1081 ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg
     1141 cgcctttcct ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg
     1201 gggcggtagc cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact
     1261 tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc
     1321 tgatgggcgg tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca
     1381 atttcctgac gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac
     1441 gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg
     1501 ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt
     1561 gggtcatttt cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg
     1621 ccgcatttgg cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc
     1681 ggctcggcga aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc
     1741 tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg
     1801 gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc
     1861 aggggcagct gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc
     1921 tcctcttcac ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg
     1981 caggcgctgc cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg
     2041 cagggcaacg agccgatcgc tgatagaggt ttatagcggc ttcgttccct tccgcaaccg
     2101 tcagcatgat ctgctccaca tgctcgcggc catagtctat caccgcccgc agcagcatac
     2161 cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac gccggacaga tgcgccttgt
     2221 gagcgtcctt ggggccgtcc tcctgtttga agaccgacag cccaatgatc tcgccgtcga
     2281 tgtaggcgcc gaatgccacg gcatctcgca accgttcagc gaacgcctcc atgggctttt
     2341 tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc tttcttcagg gccgacaatc
     2401 ggatctcgcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg tcactcccgt
     2461 actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac tgtcacgaac
     2521 ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg ggctggcggg
     2581 gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg gttatcagcg
     2641 cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg atagctggtc
     2701 tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga aaggcgggca
     2761 aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt ttagccgctt
     2821 tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc agggcctgtc
     2881 tcggtcgatc attcagcccg gctcatcctt ctggcgtggc ggcagaccga acaaggcgcg
     2941 gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatcct ccggccactc
     3001 gctgctgttc accttggcca aaatcatggc ccccaccagc accttgcgcc ttgtttcgtt
     3061 cttgcgctct tgctgctgtt cccttgcccg cacccgctga atttcggcat tgattcgcgc
     3121 tcgttgttct tcgagcttgg ccagccgatc cgccgccttg ttgctcccct taaccatctt
     3181 gacaccccat tgttaatgtg ctgtctcgta ggctatcatg gaggcacagc ggcggcaatc
     3241 ccgaccctac tttgtagggg agggcgcact taccggtttc tcttcgagaa actggcctaa
     3301 cggccaccct tcgggcggtg cgctctccga gggccattgc atggagccga aaagcaaaag
     3361 caacagcgag gcagcatggc gatttatcac cttacggcga aaaccggcag caggtcgggc
     3421 ggccaatcgg ccagggccaa ggccgactac atccagcgcg aaggcaagta tgcccgcgac
     3481 atggatgaag tcttgcacgc cgaatccggg cacatgccgg agttcgtcga gcggcccgcc
     3541 gactactggg atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt caaggaggtc
     3601 gaatttgccc tgccggtcga gctgaccctc gaccagcaga aggcgctggc gtccgagttc
     3661 gcccagcacc tgactggtgc cgagcgcctg ccgtatacgc tggccatcca tgccggtggc
     3721 ggcgagaacc cgcactgcca cctgatgatc tccgagcgga tcaatgacgg catcgagcgg
     3781 cccgccgctc agtggttcaa gcggtacaac ggcaagaccc cggagaaggg cggggcacag
     3841 aagaccgaag cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc atgggccgac
     3901 catgccaacc gggcattaga gcgggctggc cacgacgccc gcattgacca cagaacactt
     3961 gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt ggtggagatg
     4021 gaaggccggg gcatccgcac cgaccgggca gacgtggccc tgaacatcga caccgccaac
     4081 gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg caatcgacag
     4141 agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc tggcccagag
     4201 catggcgaca ctggccgacg aagcccggca ggtcatgagc cagacccagc aggccagcga
     4261 ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat gggtggagct
     4321 ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga gcgcccggag
     4381 cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt ccatgatgcc
     4441 tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac tgacaaccga
     4501 ggacggctcg atctggctgc gcttggtggc ccgatgaaga acgacaggac tttgcaggcc
     4561 ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt cagggacgcc
     4621 accaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca gaacacgcca
     4681 tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc cgagcaggag
     4741 cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga catgaaagcc
     4801 gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca ggcatgggtc
     4861 aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac gctggccagc
     4921 gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt ggcgggcttc
     4981 accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt gctgctgcgt
     5041 gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc tggccagcag
     5101 atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct cgaactgccc
     5161 gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag cgagatggcc
     5221 gggctggtca agcgcttcgg tgatgacctc agcaagtgcg actttatcgc cgcgcagaag
     5281 ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga ggccagccca
     5341 gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac cgtcagcaag
     5401 gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc accggcaccc
     5461 cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt gcgttggtac
     5521 tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga atacgaaaaa
     5581 agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt gcccggtggc
     5641 tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga tatggccagc
     5701 cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt cggccccggc
     5761 aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg acctgctggc
     5821 cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca aagggatgcg
     5881 tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt gcctggaaga
     5941 actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac caactgcctg
     6001 caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc gttcgcagca
     6061 gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt cggggcgctg
     6121 gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc acagattgca
     6181 ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat ctacctgccc
     6241 gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc gcacctcagc
     6301 gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat cggcagcctg
     6361 cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga gggccgccgc
     6421 ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc cagcggcccc
     6481 atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg ctctatcgtg
     6541 ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca gcagcaggcc
     6601 agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct gtcgagcatg
     6661 accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt cttcgtccgc
     6721 ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt caggcggcat
     6781 gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa gggggtgccc
     6841 cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc cggcgcactg
     6901 tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca gcaagctgga
     6961 cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg agccgctggg
     7021 cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta atggcctagt
     7081 gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc tggaacccaa
     7141 gtgggaggcc gtcaccgctg atgccatggt ggtcaaaggt agctatcggg cgctggcaaa
     7201 ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg actgcatcga
     7261 gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg ggtttcggct
     7321 gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc tgaacccctt
     7381 gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg acgaggtgcg
     7441 ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct ggatcgaccc
     7501 cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc cgtcagaggc
     7561 cagtggttcg accatgcgca agcgccgcca gcgggtgcgc gaggcgttgc cggagctggt
     7621 cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca cccggcccaa
     7681 ggcggcaggc tgaccccccc cactctattg taaacaagac atttttatct tttatattca
     7741 atggcttatt ttcctgctaa ttggtaatac catgaaaaat accatgctca gaaaaggctt
     7801 aacaatattt tgaaaaattg cctactgagc gctgccgcac agctccatag gccgctttcc
     7861 tggctttgct tccagatgta tgctcttctg ctcctgcagc taatggatca ccgcaaacag
     7921 gttactcgcc tggggattcc ctttcgaccc gagcatccgt atgatactca tgctcgatta
     7981 ttattattat agaagccccc atgaataaat cgctcatcat tttcggcatc gtcaacataa
     8041 cctcggacag tttctccgat ggaggccggt atctggcgcc agacgcagcc attgcgcagg
     8101 cgcgtaagct gatggccgag ggggcagatg tgatcgacct cggtccggca tccagcaatc
     8161 ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg
     8221 cgctcaaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct
     8281 atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca gacgctgcgt
     8341 tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg
     8401 ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt gcggcgttct
     8461 ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg
     8521 atcccggcat ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc
     8581 ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat
     8641 cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt cggggccgcg acactcgctg
     8701 cagagcttgc cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct
     8761 tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc
     8821 acattcggga tatttctcta tattcgcgct tcatcagaaa actgaaggaa cctccattga
     8881 atcgaactaa tatttttttt ggtgaatcgc attctgactg gttgcctgtc agaggcggag
     8941 aatctggtga ttttgttttt cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg
     9001 cttcccgccg cggtgagctc gctggagagc gtgaccgcct catttggctc aaaggtcgag
     9061 gtgtggcttg ccccgaggtc atcaactggc aggaggaaca ggagggtgca tgcttggtga
     9121 taacggcaat tccgggagta ccggcggctg atctgtctgg agcggatttg ctcaaagcgt
     9181 ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct atcggttgat caatgtccgt
     9241 ttgagcgcag gctgtcgcga atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg
     9301 tcaatcccga cttcttaccg gacgaggaca agagtacgcc gcagctcgat cttttggctc
     9361 gtgtcgaacg agagctaccg gtgcggctcg accaagagcg caccgatatg gttgtttgcc
     9421 atggtgatcc ctgcatgccg aacttcatgg tggaccctaa aactcttcaa tgcacgggtc
     9481 tgatcgacct tgggcggctc ggaacagcag atcgctatgc cgatttggca ctcatgattg
     9541 ctaacgccga agagaactgg gcagcgccag atgaagcaga gcgcgccttc gctgtcctat
     9601 tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct tgccttctat ctgcgattgg
     9661 accctctgac ttggggttga tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc
     9721 gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt tggaaagttt cattgccaga
     9781 cgggactcct gcaatcgtca agggattgaa acctatagaa gacattgctg atgaactgcg
     9841 cggggccgac tatctggtat ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga
     9901 gaacaatctg atgttgctcg aatatgccgg ggagcgaatg ctctctcaca tcgttgccga
     9961 gcacggcgac taccaggcga ccgaaattgc agcggaacta atggcgaagc tgtatgccgc
    10021 atctgaggaa cccctgcctt ctgcccttct cccgatccgg gatcgctttg cagctttgtt
    10081 tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat
    10141 tatagccgat caaatgatga gcaatgcctc ggaactgcgt gggctacatg gcgatctgca
    10201 tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg atagatcccg tcggtctggt
    10261 cggtgaagtg ggctttggcg ccgccaatat gttctacgat ccggctgaca gagacgacct
    10321 ttgtctcgat cctagacgca ttgcacagat ggcggacgca ttctctcgtg cgctggacgt
    10381 cgatccgcgt cgcctgctcg accaggcgta cgcttatggg tgcctttccg cagcttggaa
    10441 cgcggatgga gaagaggagc aacgcgatct agctatcgcg gccgcgatca agcaggtgcg
    10501 acagacgtca tactagatat caagcgactt ctcctatccc ctgggaacac atcaatctca
    10561 ccggagaata tcgctggcca aagccttagc gtaggattcc gccccttccc gcaaacgacc
    10621 cctttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
    10681 cccggtgagc gtcggaaagg cgctggaagc cccgtagcga cgcggagagg ggcgagacaa
    10741 gccaagggcg caggctcgat gcgcagcacg acatagccgg ttctcgcaag gacgagaatt
    10801 tccctgcggt gcccctcaag tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc
    10861 ttgagtccac gctagatcta tctcatctgc gcaaggcaga acgtgaagac ggccgccctg
    10921 gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac
    10981 gagcggacgc cgcagcagga gcgccagaag gccgccagag aggccgagcg cggccgtgag
    11041 gcttggacgc tagggcaggg catgaaaaa
//
