LOCUS       NZ_KU302807             4863 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Enterobacter cloacae strain SZECL1 plasmid pSZECL_e clone ST231,
            complete sequence.
ACCESSION   NZ_KU302807
VERSION     NZ_KU302807.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227287
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Du,H., Chen,L., Chavda,K.D., Pandey,R., Zhang,H., Xie,X., Tang,Y.W.
            and Kreiswirth,B.N.
  TITLE     Genomic Characterization of Enterobacter cloacae Isolates from China
            That Coproduce KPC-3 and NDM-1 Carbapenemases
  JOURNAL   Antimicrob Agents Chemother 60 (4), 2519-2523 (2016)
   PUBMED   26787700
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Du,H., Chen,L., Tang,Y.-W. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) Public Health Research Institute, Rutgers
            University, 225 Warren St, W210M, Newark, NJ 07103, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Velvet v. 1.2.10
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/06/2024 03:01:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KU302807.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="SZECL1"
                     /isolation_source="Sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /clone="ST231"
                     /plasmid="pSZECL_e"
                     /geo_loc_name="China"
     gene            complement(209..322)
                     /locus_tag="HTH07_RS00005"
     CDS             complement(209..322)
                     /locus_tag="HTH07_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            738..929
                     /locus_tag="HTH07_RS00010"
     CDS             738..929
                     /locus_tag="HTH07_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            929..1351
                     /locus_tag="HTH07_RS00015"
     CDS             929..1351
                     /locus_tag="HTH07_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2135..2422)
                     /locus_tag="HTH07_RS00020"
     CDS             complement(2135..2422)
                     /locus_tag="HTH07_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236901745.1"
                     /translation="MIKEDGSMSVYCVSYDLNQAGQNYNALYDELKKSPGWCHPLDSTW
                     LISTGETAQQLSDRLRRHLDNNDTLLVIGVTKEYAGWLTQDTWDWMRTDI"
                     /name="hypothetical protein"
     gene            complement(2780..2977)
                     /locus_tag="HTH07_RS00025"
     CDS             complement(2780..2977)
                     /locus_tag="HTH07_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172688155.1"
                     /translation="MEHLLKKAKCRGSMRFFLYQMVPLAAVVSSAVLALHSVSGWGWFL
                     FVAFMLSGADTVKKHLGNDA"
                     /name="hypothetical protein"
     gene            3096..4394
                     /locus_tag="HTH07_RS00030"
     CDS             3096..4394
                     /locus_tag="HTH07_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_045265534.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            4398..4835
                     /locus_tag="HTH07_RS00035"
     CDS             4398..4835
                     /locus_tag="HTH07_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045265533.1"
                     /translation="MSKRGRGLRSSSRKEETKSSKSIMKTTDRFISVSFRYFHNVDDIG
                     QSIDSWRVDGSLPDLIEKLVYLTANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVD
                     ENWGVIKNIGGQKRRVAGFLKDNVFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     Typing          complement(210..617)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 ggcaagatct tcttgagatc actgtgttct cgtcgtaatc tctcgctctg taaacgaaaa
       61 aaccgccttg gcgggcggtt ttttcgaagg ttccaagagt tggcgctctt agaaccaagg
      121 taactggctt ggaggagcgc agtaaccaaa acttgttctt tcagtttagc cttaaccggc
      181 gcataacttc aagactaact cctctaaatc agttaccagt ggctgctgcc agtggcgctt
      241 ttgcatgtct ttccgggttg gactcaagac gatagttacc ggataaggcg cagcagtcgg
      301 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga
      361 gtgtctggcg tggaatgaga taaacgcggc cataacagcg gaatggcacc ggtaaaccga
      421 aaggcaggaa caggagagcg cacgagggag ccgcctatca gaaatgatag gtatctttat
      481 agtcctgtcg ggtttcgcca ccactgattt gagcgtccgt ttctgtgatg cttgtcaggg
      541 gggcggagcc tatggaaaaa cggccgctgc gcggcctcct cttttcctcc taccttgatc
      601 gctcggtttt ctcgagcttt tataagaatg gtcttgccgc tcgccgcagc cgaacgaccg
      661 gagcgtagcg actgagtgag cgaggaagcg gaaaagagac tggttttaca ctgagcactg
      721 acgctctgag gcttcttatg acgaaacaac agaaaacagc gctgaacatg gctaaattca
      781 tccagaatca gtctctgctc ctgctggaga agcttaacga gcttgctctc gatgtcgagg
      841 ccgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca ctgtcctcga
      901 gactggatga attacaggat ggtaactgat ggccagagtg tctattagtg aagcggcacg
      961 gcttgttaag gttagtcgcc ccacaattta caagatgatt aacagtggga agttaagtta
     1021 cacctctgtt gtaaagcacg gtaaggctat taaagttatt gatacttcag agcttattag
     1081 agtgtttggt tctcttgatg gtgtcatcga tactgtaaaa tatgatgtaa aaagtgacgc
     1141 agaatctaca ggtgttaaca gcgttggttt acatgattta caacatagaa tcgctttact
     1201 tgaagctgaa aacgatggtc ttaaaggggc tgttaaggct cgagatgagc atattgattc
     1261 ccttcgtcag gctatgcagc ttctggagca caagcacgaa ccttcttcac caccccattc
     1321 cccgtggtgg aaattctgga aaaaatcctg accccgtcat gcttccgttt tcctctccgg
     1381 ccatcccggg gagttcctga accttcccaa aaatcgtcag atctctgaac gctcctcacc
     1441 actaaaaata cccacacccc acgcaaaatt aacattttgc agatttgtat ttaatatcag
     1501 tgggttactt tcctgaaatt tcgtttttta aattttattt atatagatcc gactctatga
     1561 cgagtcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc aggactataa
     1621 aaacaataag ttaataacga cccgactcta gacacgactc cccgtttggt tgttttttag
     1681 actcggcgac tctgggtacg actctagtga agattcgccg gtgaatcgtg tggtgagtcg
     1741 tccggcgact tgccataacg tgattgccgg ccgtcgacga acccgatttc tgaacgtgct
     1801 gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc
     1861 cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga cttaccctgg ctgaatggcg
     1921 tgaattttcg cgctatgcac ttgcgtgcat actcatgcat gccgtaaaaa cagagcctgc
     1981 gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac
     2041 gccctgaggc cgttttagcg gtgcgtacaa ttagggggtt atgttaaatc cacaccagct
     2101 acagacgtaa aaaaagcccc gcagggcttt atatctatat atctgttctc atccaatccc
     2161 atgtgtcttg ggtgagccat cctgcgtatt ctttggttac accaatcaca agcaaggtgt
     2221 cattattgtc cagatgccta cgcaaacgat ccgacagttg ttgcgctgtt tctccggttg
     2281 atattagcca tgtggaatct aaagggtggc accaccccgg cgattttttt aactcatcat
     2341 atagtgcatt gtagttttga cctgcttgat tcaggtcata agagacacag taaactgaca
     2401 tagaaccatc ctctttaatc aaatgtgggg cagcccgtta gggctgcagt cacaacttac
     2461 ttcttctttt cacgattaat tggaattttg aacatcccac cgtttggacg ggtgatccgt
     2521 ttgcccttta ctgtgatata agggcgaaaa atgtatgcct gagactcgtc tgtctgcatg
     2581 gtgcatttcc tatttttcgc cacatgagtg ttgatttttg ctatcaaaat tgtactgtag
     2641 caaccaacaa atcttgtggc tggctttatc aagctcttga ggtggtactc aagtgccgca
     2701 tttgtgtatg gtttgttacg ggttagttct ggtggtacag gctaacccac ttacttcaaa
     2761 aaacgtcgtt tttctccttt catgcgtcat ttcctagatg tttcttcact gtatcagctc
     2821 cgctgagcat aaatgctaca aagagaaacc atccccatcc tgaaaccgag tgtaaagcta
     2881 gcactgctga gctgacaact gcggctaaag gaaccatttg gtatagaaaa aatctcatag
     2941 accccctgca tttagctttt tttaacaagt gttccataac gtgctatcta ttaaacgcac
     3001 ctgttgtttt agaacgcttg tatgctatcc ttaagtcctg attttgtcaa tatttactca
     3061 ttttgttgtt taaggatggc gtaaggggtg ttcttatgat ggttaatttt agtctttcta
     3121 acgcattttc ctttatggat gctcaaaata tgagcatgtt ggcggtgtca tccgcgaaag
     3181 acgacataaa cttggctaac gtggtttcag tgagtgatgt tcggatactg aaatctgctc
     3241 tgatatttgg ggctaacgct agcgggaagt ctaatctcat gaaaacaatt tctttaatga
     3301 aagatattgt tttgcattcc gtgcaagcta ttgaaagtag cattacaaag aacgtaatac
     3361 cgtttttgct tgatgaggat ggaccaaata aaccatctga aatggaaatg acttttattt
     3421 cagatggggt taaatataga tatggattgt ctatttttca ggggaaaata aaagaagaat
     3481 ggttgtacta cacaccaaaa tcaagagaaa ccattctctt tgaaagagat ggtctttcta
     3541 ttgaggtaaa taaggcggct tttagtgagt caaacctttt tatcaaagaa ggggttattc
     3601 aaaaaacaag ggaggatgtt ccatttgttt cggttgttgc tggttttaat ggtgagcatt
     3661 caaaacgagt agttaattgg tttaacaata tcgtattcct ttctggttct aatgaaggga
     3721 tgtttacagc aattaccatg aagttaataa atgagtctga ggattttaag gcttggctaa
     3781 aaaaaatact tccagcattc cagattaatg atatttacct cgatgaggtc gcacctgatt
     3841 ttccatttgg aaatataaaa acagatgatg ataatttaaa aaatttattt agttcaatac
     3901 aaaattttgc aaaaaaccag aaggtttttt ctcttagagt agtgaaaaaa acagctagcg
     3961 gtgagatttc agtacctatt gattttgagt ctgagggtac taaaaagata gtttatcttc
     4021 ttggtccaat ctacaatgcc atgataaacg gtaacttatt gttagttgat gagtttgatt
     4081 ctaagtttca cacccttttg tctaagtttt tgtttagaat attccatgcg caaagcgagg
     4141 ggcttagcca aataatagct gctgttcagg atgttaatct aatgaacact gattgtttta
     4201 gacgtgacca gatttggttt gttgataaag atataaaatc aggaagtagt catctctatt
     4261 ctttggttga gtataaagag aagcagcgaa ctttaaaatc atcgtatggg catgattatt
     4321 tatctggtgc atttgatgct atcccattgt ttgataattt agatgaattt gaaaaactta
     4381 tgcctgaggg atgatttatg tcaaagagag gccgcggtct tcgtagctct tcgaggaaag
     4441 aggaaacaaa atcctcaaag agcattatga aaactacaga tagatttatt tctgttagtt
     4501 ttagatattt tcataacgtc gatgatattg gtcagtctat tgattcttgg agggttgacg
     4561 gcagtttgcc agatttaatt gaaaagcttg tttatcttac agctaatggg ataacaaaag
     4621 tcagctctga tggtattttt actaactaca ctcgatttcc agattcagca gttaatgatt
     4681 ttaaatgccc tgatgaaata tcagttgatg agaactgggg tgttataaaa aatataggag
     4741 gccagaaaag aagggttgct ggttttttaa aggataatgt gttttatata gttttttttg
     4801 ataaggatca tcggttttgg aagagcagga aataggctta gttacgattg tactgtatgt
     4861 tga
//
