LOCUS       NZ_KU302806             4858 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Enterobacter cloacae strain SZECL1 plasmid pSZECL_d clone ST231,
            complete sequence.
ACCESSION   NZ_KU302806
VERSION     NZ_KU302806.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227288
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Du,H., Chen,L., Chavda,K.D., Pandey,R., Zhang,H., Xie,X., Tang,Y.W.
            and Kreiswirth,B.N.
  TITLE     Genomic Characterization of Enterobacter cloacae Isolates from China
            That Coproduce KPC-3 and NDM-1 Carbapenemases
  JOURNAL   Antimicrob Agents Chemother 60 (4), 2519-2523 (2016)
   PUBMED   26787700
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Du,H., Chen,L., Tang,Y.-W. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) Public Health Research Institute, Rutgers
            University, 225 Warren St, W210M, Newark, NJ 07103, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 03:11:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KU302806.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="SZECL1"
                     /isolation_source="Sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /clone="ST231"
                     /plasmid="pSZECL_d"
                     /geo_loc_name="China"
     gene            971..1294
                     /locus_tag="HTG49_RS00005"
     CDS             971..1294
                     /locus_tag="HTG49_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1503..>1937
                     /locus_tag="HTG49_RS00040"
                     /pseudo=""
     CDS             1503..>1937
                     /locus_tag="HTG49_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1972..2463
                     /locus_tag="HTG49_RS00015"
     CDS             1972..2463
                     /locus_tag="HTG49_RS00015"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2470..2685
                     /locus_tag="HTG49_RS00020"
     CDS             2470..2685
                     /locus_tag="HTG49_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3157..3648
                     /locus_tag="HTG49_RS00025"
     CDS             3157..3648
                     /locus_tag="HTG49_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(3705..4133)
                     /locus_tag="HTG49_RS00030"
     CDS             complement(3705..4133)
                     /locus_tag="HTG49_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            4418..4846
                     /locus_tag="HTG49_RS00035"
     CDS             4418..4846
                     /locus_tag="HTG49_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          685..858
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(216..761)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.909"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          1285..2777
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatc ccttgagatt cgttgaccct cgtcgtaatc tcttgcggtg aaaacgaaaa
       61 aaccacccgg caaggtggct ttttcgaagg ttcgcaagag ttgacgctct tttgaaccgc
      121 ggtaactggc ttggaggagc acagtcacca aaactgtcct ttcagtgtag ccttatcgca
      181 cccgaaactt caagactctc ctcctctaaa cctgttacca gtggctgctg ccagtggcgc
      241 tttgtcgtgt ttttccggat tggactcaag ttgatagtta ccggataagg cgcagcggtc
      301 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaact
      361 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
      421 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
      481 aagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg
      541 ggggcggagc ctatggaaaa acggcttcgc cgcggcctta tcgtttccct gttaagtttc
      601 ttcctggcat ctaccaggaa tttccgcccc attcgtaagc ccacaccgct cgccgcagcc
      661 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga atatatcctg tatcgcatat
      721 tctgctgacg cgccggtgcc gccttttttc tcctgccaca tgaagcactt cactgattcc
      781 cgcacccgtg ccaatataat aagccagtat acactccgct agcgctacgt gactggttca
      841 gggctgcgcc ccgaaacccg ccaaaagcct gacgcgcctg cggcttgtcc aaccccgcgg
      901 cgaccgggaa aggctttccc gcccgtcccg gcgttatcag gaggccggtt tgtcagagaa
      961 acggagcaag atgctcacca tgtgggtgac ggaggacgag caccggcggc tgcttgagcg
     1021 ctgtgacggc aggcagctgg cggcgtggat gcggcagatc tgcctggacg aaaagccggc
     1081 acgttccgga aagctcccct cgatctcgcc ggcgctgctg cgccagcttg ccggcatggg
     1141 caataacctg aaccagatag cccgccgtgt taacgccggc ggcggcaccg gccatgacag
     1201 ggtacagatt gtggcggcgc tgatggccat cgatgccggc cttgagcggc tgcggcacgc
     1261 cgttcttgag aaaggaacgg acgatgatcg ttaaatttca tccgcgcggc cgcggcggtg
     1321 gcgccgggcc ggtggactat ctgctgggta aagaccggca gcgtgaaggc gccaccgtcc
     1381 tgcaggggaa gccggaagaa gtccgggagc tgattgacgc gtcgccttac gttaagaaat
     1441 acacctccgg cgtcctgtcg tttgcggagg ccgatctgcc tcccggccag cgggaaaaac
     1501 tgatggccag cttcgagcgg gttctgatgc ccggactcga taaagatcag tacagcattc
     1561 tgtgggtgga gcacgcggac aaggggcgtc tggagctgaa ctttctgatc cccaataccg
     1621 agctgctgac cggcaggcgg ctccagcctt actacgaccg cgccgaccgg ccgcgcatcg
     1681 atgcctggca gactatcgtg aacggcagac tggggctgca cgacccgaac gcgccggaaa
     1741 accgccgggc gctggtcacg ccgtccgggc tgccgaaaac gaagcaggag gccgcagaag
     1801 ccattacccg gggactgctg acccttgctt cgtccgggga gctgaaaagc cgtcaggatg
     1861 tcactgacac tcttgaaggt gcaggttttg aggtggtgcg caccacgaaa agcagtatca
     1921 gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatc tatgagcagt
     1981 cttttaacgc tggcgaagga cttagagcag caatcgaaag cgcagcagca gagtaccggc
     2041 gagatgctga aagccgcatt cagcgagcac gagcagtctg tcagagcgga actgagcgca
     2101 agcgcgagga gaatcagcga cgccatcctc gcccacgagc agagtatgag cgaggcgatg
     2161 gagaaaaacc ggcggagcgt actgcggact gccgggcgga catggctcac catcctgatg
     2221 gtgtcagcgc tgctgatcgg cacgagcggg agcattctgt ggtggcaggg gcagcagata
     2281 accgacaatt acacgcatct tcgtcagcag gaagataccc tggcgaaaat gaccgcccgg
     2341 acgtggggag tgcgctatca ggagagcagc gacggccgga ggtttcttat cctgccgccg
     2401 gggatgcaaa ccgaagccat cccctacgac gggacgacat ggatccgcct gaaacaggag
     2461 tagcggaaaa tgacagagct ggaaaagcag ttgctgagcg cattagagca gctacagcag
     2521 gactactcgc aaaggctgga cgaatgggag agcgccttcg cggaatggcg gagcatgtgt
     2581 ggtcttatgc aacgggagaa cgcgatgctg aacgagcgcg tgtcggactt gagcacgcag
     2641 gtgctgagtt taagcgagca gctgcgccgc ttgtccggga actgaatgcc atcgagctcc
     2701 ggcagaggga gaatgagtat cagaaaacac tggagctgga gcgccagccg cagaaaacct
     2761 accgcgggcc gacactttaa accatccagg cagctgacaa cccgcacgaa gaggggctct
     2821 tatttctccc gctaaatagg ttcgtgatca aaaaggtcac gacttattca ggagcgggaa
     2881 atgaagctgt taaatcttat tgaatcattg cttaactgcc tgtatctggc ggcggagctg
     2941 tgggactggc tggagaagca tttgtcggtt tttaaggcat tggcgcggat tttagtgtga
     3001 ctttaacatc catgcatctg gctgcatgac tatgcaccag tgtaatattc agcggctgcg
     3061 tttctggtgt tttttcgtta tgtctgtcgc cactttctcg cgtctggcac cggaaacgca
     3121 gccaaccccg tttcagcggt gcgggcaaca gatgttatgg taaatcaacc gtccgttgat
     3181 aaaggttctg ataataccca tgtaggtgat gttgtttgct ttttatctac acactgcctg
     3241 atggagctat ttatgaatgc tgatgaagtt acacgaattc ttgcgcaatc gagtcaaaat
     3301 gattggattg tggatgatga gacaggggcg tttacttata aaaatgattt agcactgcac
     3361 attaagagag ctgattacga cgactacaac tcgtttaatg agccttgggc tgttcgtcat
     3421 cctgatagta atgcgtgttc agtcgagtac accgttcact atggttcttc gtttgttgat
     3481 agaaaaacct tggtttccgt cgatggtcat cgtgcaacct tgcctatgcc tgcatccgca
     3541 acggatctaa acgtgtcatc ggctgatgtg aatttcgcca aaattgtaaa cattggcgat
     3601 cgcgttgatg aatacattca acgttctggt cttacgatta agccctaatt taacgataag
     3661 cccggcctcc aaatgcgggc tgttcctgca tgtaaccagc ataactaatc ggcttttttc
     3721 cttactgtat atattggctt tttatgtcgc ggctttctta cttgctcgga cacagaagga
     3781 ccaattatgt ctaaataatc tacaagcttt gtaaggtcgt atatgttgcg gccaccatat
     3841 cggttgtcgc tttttgatgt cggtttttta tctaagagat tggctttttc taggtcatta
     3901 atagcccttt gcaccgtcct tactgacacc cccatccgta tagccaggct ctccattgag
     3961 gggtgcggcc actcctgtag attccaccag tgtactatga gattcagcaa ggtattgaat
     4021 gctacagggc tcaatttctt ctcattttgt ataaaaaaca acacagtagg tactgcagtc
     4081 catcctaatg acagacctcc agccttccat tttttttcta ttgcgtaact cattgattat
     4141 cctgtaaata ttttttttga gatatatata atataagtat tcatatgaat agggtgacac
     4201 cagcgccatt attacacaac tataaaaggc taatctgaga acagaaaaac atttctatct
     4261 gcattaaata cggagttagt gatatgaaaa ccaaacaaca tcatgatctt gtaaaagata
     4321 ctgaaatatt tggtgtatcc ttgtctctgt tgatttccag agcgaatgag agatctgtta
     4381 caatgccatc tggtctaagt agggagcaga gaagagcatg ggcagcggat caagcacgca
     4441 aaatccacaa ttgaatgttg tctctttctc tgagacattc aatctttatt acacatcccg
     4501 cttttctcaa aaaacactcg ataaaatcga tgacttcatc gaacatttcg agaagtacaa
     4561 tctttttggc gatgccttaa aaggccatcc ggcttggatt ggcaaggtat cgccatcttg
     4621 gaacgtgcct gacagttacg aaaacaaaga agctattgaa gaacatgcca gggctaataa
     4681 attgtggcat gcccatatag gcgatcccgt tttcatcgat acatttcacg ggaagtacaa
     4741 agtctctgat tgggtcatcc acttccaacg tctagcgccg aaccatataa aactgttaga
     4801 gcttgggtat catgacccta tgcaactccc ccaaaatttc gaatgatctc ttgttcgg
//
