LOCUS       NZ_KT988306             6779 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Shigella sonnei strain c8225 plasmid pABC-3, complete sequence.
ACCESSION   NZ_KT988306
VERSION     NZ_KT988306.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226430
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Avila,B., Miranda,A., Diaz,P., Rivas,L., Ulloa,M.T., Hermosilla,G.,
            Weil,F.-X., Del Canto,F., Salazar,J.C. and Toro,C.
  TITLE     Plasmid-borne dfrA14 trimethoprim resistance gene in Shigella sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Avila,B., Miranda,A., Diaz,P., Rivas,L., Ulloa,M.T., Hermosilla,G.,
            Weil,F.-X., Del Canto,F., Salazar,J.C. and Toro,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2015) Programa de Microbiologia y Micologia,
            Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad
            de Chile, Independencia 1027, Santiago 8380453, Chile
COMMENT     ##Assembly-Data-START##
            Sequencing Technology             :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 02:12:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KT988306.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="c8225"
                     /db_xref="taxon:624"
                     /plasmid="pABC-3"
                     /geo_loc_name="Chile: Santiago"
                     /note="Clinical isolate"
     gene            97..912
                     /gene="sul2"
                     /locus_tag="HPF46_RS00005"
     ARG             98..909
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            973..>1500
                     /locus_tag="HPF46_RS00010"
                     /pseudo=""
     CDS             973..>1500
                     /locus_tag="HPF46_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            1521..1994
                     /gene="dfrA14"
                     /locus_tag="HPF46_RS00015"
     ARG             1522..1991
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <2069..2344
                     /locus_tag="HPF46_RS00020"
                     /pseudo=""
     CDS             <2069..2344
                     /locus_tag="HPF46_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     gene            2344..3180
                     /locus_tag="HPF46_RS00025"
     CDS             2344..3180
                     /locus_tag="HPF46_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<3287..3691)
                     /locus_tag="HPF46_RS00030"
                     /pseudo=""
     CDS             complement(<3287..3691)
                     /locus_tag="HPF46_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(3735..4157)
                     /locus_tag="HPF46_RS00035"
     CDS             complement(3735..4157)
                     /locus_tag="HPF46_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4342..4782
                     /locus_tag="HPF46_RS00040"
     CDS             4342..4782
                     /locus_tag="HPF46_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4841..5332)
                     /locus_tag="HPF46_RS00045"
     CDS             complement(4841..5332)
                     /locus_tag="HPF46_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176703128.1"
                     /translation="MAGDLRRLRGDRDKKSVPHCIVHACMNGLFFLTTELHRSFSSYIT
                     FTSLIHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAPATLHGHAINCVPVH
                     RFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVFGWFVACFTGFPSETP
                     "
                     /name="hypothetical protein"
     gene            complement(5538..5687)
                     /locus_tag="HPF46_RS00050"
     CDS             complement(5538..5687)
                     /locus_tag="HPF46_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            6258..6416
                     /locus_tag="HPF46_RS00055"
     CDS             6258..6416
                     /locus_tag="HPF46_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             2345..3177
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2100..2344
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     ARG             974..1515
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.43"
                     /coverage="67.42"
     Typing          complement(5723..5896)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          5820..6425
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc atccgtatga
       61 gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct catcattttc
      121 ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct ggcgccagac
      181 gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat cgacctcggt
      241 ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc
      301 gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga cagttatcaa
      361 cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt
      421 tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg
      481 cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga catcatggat
      541 cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc
      601 aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc cgaaacctcg
      661 ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg
      721 tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg
      781 gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt catccgcaca
      841 cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa agaaaccgca
      901 agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg
      961 aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg
     1021 cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc
     1081 gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt
     1141 tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag
     1201 ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg
     1261 gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg
     1321 gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg
     1381 gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgcag
     1441 ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc
     1501 gttaacccag gatgagaacc ttgaaagtat cattgatagc tgcgaaagcg aaaaacggcg
     1561 tgattggttg cggtccagac ataccctggt ccgcgaaagg ggagcagcta ctttttaaag
     1621 cattgaccta caatcagtgg cttctggtgg gtcgcaagac gtttgaatct atgggcgcac
     1681 tccccaatag gaaatacgcg gtcgttaccc gctcaggttg gacatcaaat gatgacaatg
     1741 tagttgtatt tcagtcaatc gaagaggcca tggacaggct agctgaattc accggtcacg
     1801 ttatagtgtc tggtggcgga gaaatttacc gagaaacatt acccatggcc tctacgctcc
     1861 acttatcgac gatcgacatc gagccagagg gggatgtttt cttcccgagt attccaaata
     1921 ccttcgaagt tgtttttgag caacacttta cttcaaacat taactattgc tatcaaattt
     1981 ggaaaaaggg ttaacaaagc tatgcaatcg acggcaaaaa gcttcgttcg cttcgcgcac
     2041 tacgcctttt tccgcgattg atagcgacga tatggttgtt tgccatggtg atccctgcat
     2101 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     2161 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     2221 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     2281 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     2341 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     2401 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     2461 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     2521 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     2581 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     2641 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     2701 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     2761 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     2821 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     2881 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     2941 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     3001 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     3061 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     3121 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     3181 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     3241 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gaccccaaac aggaaacgca
     3301 gctgaaacgg gaagctcaac acccactgac gcatgggttg ttcaggcagt acttcatcaa
     3361 ccagcaaggc ggcactttcg gccatccgcc gcgccccaca gctcgggcag aaaccgcgac
     3421 gcttacagct gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga acccgtagaa
     3481 agccatgctc cagccgcccg cattggagaa attcttcaaa ttcccgttgc acatagcccg
     3541 gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga
     3601 tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc
     3661 ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac
     3721 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
     3781 agacattgcc agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac
     3841 atgttattgc gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca
     3901 gaattaatat tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag
     3961 gatgcagtat aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa
     4021 ctacctcccg aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg
     4081 tgaccaaaac caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg
     4141 acttttaaaa acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat
     4201 actacgcccg tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg
     4261 gcagcctcag accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac
     4321 acaggagaca ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca
     4381 ggaaagtcgc gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca
     4441 ggaaaaaatg atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg
     4501 cgcacttctg agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag
     4561 gcggaaaatg ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt
     4621 accctaatgc ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag
     4681 agggaacaac tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag
     4741 cggggattct ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga
     4801 tttaccataa catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga
     4861 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
     4921 ttacggcatg catgagtatg cgcacaagtg catagcgcgg aaattcacac cattcagccg
     4981 gggtaagtcc tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta
     5041 ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga
     5101 agggggtgat gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg
     5161 taaaagtgat gtataagtga tgtataagtg atgtaaaagt gatgtatgaa ctgaaggagc
     5221 gatgtaattc tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag
     5281 gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt
     5341 atttttcaac ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca
     5401 tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa
     5461 tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac
     5521 gcgggcaagc gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg
     5581 gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga
     5641 agctgcgtat cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca
     5701 gtagctgaga ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta
     5761 gcggagtgta tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat
     5821 gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt
     5881 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
     5941 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
     6001 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
     6061 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
     6121 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     6181 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
     6241 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
     6301 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
     6361 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
     6421 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
     6481 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
     6541 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
     6601 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
     6661 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
     6721 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaac
//
