LOCUS       NZ_KT896499             7747 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Klebsiella pneumoniae strain I169 plasmid pKPSH169, complete
            sequence.
ACCESSION   NZ_KT896499
VERSION     NZ_KT896499.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226420
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Kaplan,E., Sela,N., Doron-Faigenboim,A., Navon-Venezia,S.,
            Jurkevitch,E. and Cytryn,E.
  TITLE     Genomic and Functional Characterization of qnr-Encoding Plasmids
            from Municipal Wastewater Biosolid Klebsiella pneumoniae Isolates
  JOURNAL   Front Microbiol 6, 1354 (2015)
   PUBMED   26696974
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   Kaplan,E., Sela,N., Doron-Faigenboim,A., Navon-Venezia,S.,
            Jurkevitch,E. and Cytryn,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2015) Plant Pathology and Weed research, ARO,
            volcani center, POB 6, Beit-Dagan, NA 50250, Israel
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: a5_assembly v. 20150522
            Assembly Name                     :: pKPSH169
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009967055.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 02:03:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10
            CDSs (total)                      :: 10
            Genes (coding)                    :: 9
            CDSs (with protein)               :: 9
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KT896499.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="I169"
                     /isolation_source="Shafdan Dan Region Wastewater Treatment
                     and Reclamation Project site"
                     /db_xref="taxon:573"
                     /plasmid="pKPSH169"
                     /collected_by="Eddie Cytryn Lab"
     gene            join(6895..7747,1..32)
                     /locus_tag="HTG41_RS00005"
     CDS             join(6895..7747,1..32)
                     /locus_tag="HTG41_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_172686320.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIAEN
                     LTLEPIMGKRLNVMDEEHLRRVIEYSAGARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELLEVPNGKKG
                     GSRAGAASSCASA"
                     /name="helicase RepA family protein"
     gene            <2..160
                     /locus_tag="HTG41_RS00010"
                     /pseudo=""
     CDS             <2..160
                     /locus_tag="HTG41_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010370216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein A"
                     /name="replication protein A"
     gene            147..1028
                     /gene="repC"
                     /locus_tag="HTG41_RS00015"
     CDS             147..1028
                     /gene="repC"
                     /locus_tag="HTG41_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_012954629.1"
                     /translation="MSKHDLTHARHDPAHCLAPGLFRSLKRGERKRGKLDVVHDYGDGK
                     RIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAFQQDA
                     MVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYASDEQ
                     DGKLYVALNPLIARAVMGGAQHVRIDMDEVRALQTDPARLMHQRLCGWIDPGKSGRVEL
                     DTLCGYVWPGQATNPNTLKTRRQAARRALPELAALGWAVSEYAKNKFEIGRPKATHLLG
                     PAPKTQEISHEK"
                     /name="repC"
     gene            1018..1269
                     /locus_tag="HTG41_RS00020"
     CDS             1018..1269
                     /locus_tag="HTG41_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064163397.1"
                     /translation="MRNDWETPPTVEVRIPVKLNSDSGICEHHFRKVCGQGFFEQKLGF
                     EGRHLFDSLNKPAAKRSAAVVDPRRWNRTVHLTSEYVL"
                     /name="hypothetical protein"
     gene            complement(1572..2228)
                     /locus_tag="HTG41_RS00025"
     CDS             complement(1572..2228)
                     /locus_tag="HTG41_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS2"
                     /protein_id="WP_012537714.1"
                     /translation="METYRHTYRHHSFSHQDLSDITFTACTFIRCDFRRANLRDATFIN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIELRECDLKGANFSRANF
                     ANQVSNRMYFCSAFITGCNLSYANMERVCLEKCELFENRWIGTHLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIASWQQEQLLEALGIVVFPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS2"
     gene            complement(3043..3510)
                     /locus_tag="HTG41_RS00030"
     CDS             complement(3043..3510)
                     /locus_tag="HTG41_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012954630.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYEPSGGLI
                     ENLTDEEKTELQSYIETERQAAEKRSRVYYAKSAASRIVEVADTIKAGDFEPSEAWAAD
                     TWAAIEALTKAMRKAGYPKLRKAPQKAADAPMPGQAGLPFGDAPETPESAS"
                     /name="hypothetical protein"
     gene            3968..4399
                     /locus_tag="HTG41_RS00035"
     CDS             3968..4399
                     /locus_tag="HTG41_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_012537712.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPAANREQWAELARTAANLNQIARHLNEGQRGDGERIGKA
                     LGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDESEG"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(4590..4946)
                     /locus_tag="HTG41_RS00045"
     CDS             complement(4590..4946)
                     /locus_tag="HTG41_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043849886.1"
                     /translation="MQTLARRFVARSHGHVDLLVGQLPALGPRIAQPRRQGQAVGMLKL
                     LGRLDGAAGFPLAQPNFPVQPYPAGHDVDVILVRVLVAHCHPRRIGLVKAHALHEVSRH
                     GFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            5155..5838
                     /locus_tag="HTG41_RS00050"
     CDS             5155..5838
                     /locus_tag="HTG41_RS00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043849885.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSLSLMHEDLKRLPVAVS
                     TETAQTLEPLARLQQDVTQVLEAYDKVTAIQRKTLDELTQQMSASAAKAFEQKAGELDK
                     TISDLSNSLSGLETSISSMKQTAEQVADLPGNLVSAQKDMTQAANKLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            5874..6824
                     /locus_tag="HTG41_RS00055"
     CDS             5874..6824
                     /locus_tag="HTG41_RS00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043849884.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGIS
                     PDTIEHAEKAGMVRYADGGVLFVGYDRTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRA
                     ERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEHGKDLADMNARQV
                     AEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR"
                     /name="hypothetical protein"
     ARG             complement(1576..2228)
                     /name="qnrS2"
                     /gene="qnrS2"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS2"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          6896..7747
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.476"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4384..6821
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.401"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccggcgcggc ttcttcgtgc gcttcggcgt gagcaaacag aactacgacg ccaccccgct
       61 tgaccagtgg tatgagcgcc gagacggcgg cgtgctggtt ccggtggaac tgctggaagt
      121 gccgaacggc aagaaggggg gcagccgtga gcaagcatga cctgacccac gcccggcacg
      181 acccggcgca ttgtctggcc cccggcctat tccgcagcct caagcgtggc gagcgcaagc
      241 gcggcaagct ggacgtggtg catgactatg gcgacggtaa gcgcatcgag ttcagcggcc
      301 cggagccgct gggcgctgat gacttgcgca tcctgcaagg acttgtggca atggctgggc
      361 cgggtggcct tgtgctgtca cctgagccgc agaccgaggc cgggcagcaa ctccggctgt
      421 tccttgagcc gaaatgggaa gccttccagc aggatgcgat ggtggtcaag ggcagctatc
      481 gagcacttgc ccgcgaaatt ggctacgcca accccgacga cactaagccg gttcgggagt
      541 gcatcgagcg gctttggaag gtgtccatca tcgcccagaa cggcaggaag cggcagggct
      601 tccgcctgct gtccgagtac gccagcgacg agcaggacgg aaagctgtat gtagccttga
      661 atcccctgat tgctcgcgcc gtcatgggtg gcgcgcagca tgtccgcatc gacatggacg
      721 aggtgcgggc gctacagacc gacccggcgc ggctgatgca ccagcgccta tgcggctgga
      781 tcgaccccgg caagtccggg cgggtggaac tggacacgct ttgcggctac gtctggccgg
      841 ggcaggcaac caaccccaat acgctcaaga cgcgacgcca agcggctcgc agggcgcttc
      901 ctgagcttgc cgcattgggc tgggcagtca gcgaatacgc aaaaaacaag tttgaaatcg
      961 gcaggccgaa agcaacgcat ctgctcggcc cagcacccaa aacacaggaa attagccatg
     1021 agaaatgatt gggaaacgcc gccgacagtc gaagtgcgta ttccggtgaa gttgaacagt
     1081 gattccggaa tctgtgaaca ccatttccgg aaagtatgcg gacagggatt cttcgagcag
     1141 aagctgggct ttgaggggag gcatcttttc gactcactga acaagccagc agccaagcgc
     1201 agcgcggcag ttgtggatcc aagacgttgg aacaggacgg ttcatctgac atcagagtac
     1261 gtgctctgag gtgttgtagg agaggaataa agtttcatac taagcagtca agtaaccctg
     1321 tttgtaccgt tctaaatgat actcacccag aaataaagat tcatactaat tcctactaca
     1381 aaatctgagt cacgctacaa ctgagtaata aagtatcata ctggttgtct tgagcatcca
     1441 cagattgatt ggccccattc aaacacgttt ctggacaaga atgagctaga agtattgatc
     1501 tagctctggt gatttgaatt agaaaatgta ttaatgttgt gtatagttta tcagcaggta
     1561 ttcgtaagca tctagtcagg aaaaacaaca atacccaacg cttcgagaag ctgttcttgt
     1621 tgccagctgg caattttgat acctgatgta tcgacttttc gaggatctaa accgtcgagt
     1681 tcggcgtgac ataaattagc accctgtagg ctaaactgtc cccagacatc ttcagaaaaa
     1741 acacctcgac ttaagtctga ctctttcagt gatgcgcccg cgaggtgagt ccctatccag
     1801 cgattttcaa acagctcaca tttttctaaa cagacccgct ccatattggc ataagacagg
     1861 ttacatccag taataaaggc tgagcaaaag tacatacgat tactcacttg attggcaaag
     1921 tttgctcggg aaaagttggc cccttttaaa tcacactcac gtaactcaat accgtagcaa
     1981 ttggcgttac taaagtttgc catcgcaagc tggcattgtt ggaaacttgc atcgcgaagg
     2041 tctgcgacat caaaatggca accttcgata tcaccctgtt caatgaactt gcagttaata
     2101 aatgtcgcat cacgcaagtt agcacgtcga aaatcgcatc ggataaaggt gcaagcagtg
     2161 aaagtaatat cacttagatc ttgatgtgaa aaactgtggt gtcgatatgt gtgacggtag
     2221 gtttccatgt tgactcctat gatgaactgg acgcaacact accatcaaag ccacatcaca
     2281 tcaccaaaat aaaaacacta aaaatcaaga tcttaaaaac cctcattttg cctattttaa
     2341 gggggtaaaa tagcgccctg agcctctatg ttaacgttca aagtacgcgg caccattgaa
     2401 aaaagttatc cgcttaaaaa gccacagaga aaaggaaccc aatagattac tgatgtccgc
     2461 cattaggtaa agtaacctta aacggaagcc tgcaatttga agtagataca aggtctaaga
     2521 aggaatggtt ggctgtttta ttttattgta ctcggcatta agcccaattt ctttagcgtt
     2581 acgataagct agcatggcta caccgacccc gctaggctga ttgggaagcg ttgcctgatc
     2641 tactcaggag ggcgcttcga taacgtagcc attgagccgg aaaccggcga gcactggggc
     2701 agcgtgatct ggtgcgagta tgtgtgaccc cctgtttaac tccacccaac ccccacggta
     2761 actccaccca acccccacgg taactccacc cgccccgatc tggaaaaccc agacggggcg
     2821 cggatttgct ggcgattcgc aaaatccatc caagattctc taagaatctc cactaggcgc
     2881 agcacagccc gcgccccttc ggcgcggtct ggcattgcct caaatgcccg gccaagacca
     2941 tgcgcaaggc gaacacctct ccccccgcct tcggcgaccc cctccggggg taaagggcca
     3001 gcggggagag ggggggggtg ccggtttccc ggcctaaacc cctcaggagg cgctttctgg
     3061 cgtttctggc gcatccccaa agggcaaccc cgcctgaccc ggcatcggcg cgtctgcggc
     3121 tttctggggc gctttgcgga gcttcgggta gcccgccttg cgcatggcct tggtcagcgc
     3181 ctcgattgcc gcccaggtat ccgccgccca tgcctcggac ggctcaaagt ccccggcctt
     3241 gatcgtgtcg gcaacctcaa caatacgact ggcggcagac ttagcgtaat acactcggct
     3301 tctcttttct gctgcctgtc gttctgtttc gatgtatgac tgtagttctg ttttttcttc
     3361 gtctgtcaga ttctcaatta ggccgcctga cggctcgtaa gaatatacgt caatagaccc
     3421 catcatcttg acgatggcgc ggcgtttttc cttgtcatac ccccggtagg cgagcacctg
     3481 aacccggcga ccttggcgct taaactgcat ggcggtttct ccgtttgact gtgtgattat
     3541 attatagttc gacagtcgca cgctgtcgag tgtttattgt cggcctattt ttcaatccga
     3601 ccgtcgcact caaataggtt tgcactgtcg gattgagtaa cgctcgacag tctgccgttc
     3661 ttgcgatacc atagttgcat tgtatcagtt cgccccggcg ccttgcccac cccctctccc
     3721 cgctggccca ttaccgccct tgggcggtcg ggccgagagc gcagcgagcg ggccgggtgg
     3781 gtggctgttc ggtgcttgca tggagatgtg ctattatggt atttgcgcgg tggtttggca
     3841 tggagcaagg tgtaacctac gcttcgcttg gttctcaccc tccggccttc ggcctacgcc
     3901 cccgctccct tcggtcgggg ggttgctgcg cgtttcgctt cgcttcactt actggagggc
     3961 ttcgcccatg aacactgacg aatccaagcg ccgccgccgt gggcctgcgc cgctcgacgc
     4021 cacggacaag cgcgggcata ccgtttcggt gcgcctcaac gatgccgagc ttgcgcggct
     4081 ggattcgcag cgtgacgcgg tgcagatgca gcggggcgag tacctacgcg ccgccgctct
     4141 gcatcgcctg ccgcccacca tcccggcagc gaaccgggaa cagtgggcag agttggcccg
     4201 cacagcggcc aacctgaacc agatcgcccg gcacctgaac gaagggcagc ggggcgacgg
     4261 ggagcgcatc ggcaaggcgc tgggcttgca gttgagcgcc gagcttgcca actgcctgcg
     4321 attgctggtt ctggtgcgcg gcgacttgat aggcgtcaag cacgactcgg aggacgtgga
     4381 agatgaaagc gaaggttagc cgtggtggcg gttttcgcgg tgcgctgaac tacgtttttg
     4441 acgttggcaa ggaagccacg cacacgaaaa acgcggaacg tgtcggcggc aacatggccg
     4501 ggaatgaccc ccgcgaactg tcgcgggagt tctcagccgt gcgccagttg cgcccggaca
     4561 tcggcaagcc cgtctggcat tgctcgctgt cactgcctcc cggtgagcgc ctgagcgccg
     4621 agaagtggga agccgtgacg actgacttca tgcagcgcat gggctttgac cagaccaata
     4681 cgccgtgggt ggcagtgcgc caccaggaca cggacaagga tcacatccac atcgtggcca
     4741 gccgggtagg gctggacggg aaagtttggc tgggccagtg ggaagcccgc agcgccatcg
     4801 aggcgaccca agagcttgag catacccacg gcctgaccct gacgccgggg ctgggcgatg
     4861 cgcgggccga gcgccggaag ctgaccgaca aggagatcaa catggccgtg agaacgggcg
     4921 acgaaccgcc gcgccagcgt ctgcaacggc tgctggatga ggcagtgaag gacaagccga
     4981 ccgcgccaga actggccgac cggctacatg ccgcaggcgt aggcgtccgg gcaaacctcg
     5041 ccagcaccgg gcgcatgaac ggcttttcct tcgaggtggc cggagtgccg ttcaagggca
     5101 gcgacttggg caagggctac acatgggcgg gactacagaa atcaggggtg acttatgacg
     5161 aagctagaga ccgtgcgggc cttgaacgat tcaggcccac agttgcagat cgtggagagc
     5221 gtcaggacgt tgcagcagtc cctgagcctg atgcacgagg acttgaagcg cctaccggtc
     5281 gcagtctcga ccgagacggc gcagaccttg gaaccgctgg cccgactcca acaggacgtg
     5341 acgcaggttc tggaagccta cgacaaggtg acggccattc agcgcaagac gctggacgag
     5401 ctgacgcagc agatgagcgc gagcgcggcg aaggcgttcg agcagaaggc cggggagctg
     5461 gacaagacca tctccgacct gtccaacagc ctgtcagggc tggaaacgag catcagcagc
     5521 atgaagcaga ccgcggagca ggtggcggac ttgccgggca atctggtgag cgcacagaag
     5581 gacatgacgc aagccgccaa caaactgacc gaggcagcga acgagacgcg cccgcgcctt
     5641 tggcggcagg cgctggggct gattctggcc ggggccgtgg gcgcgatgct ggtagcgact
     5701 gggcaagtcg ctttaaacag gctagtgccg ccaagcgacg tgcagcagac ggcggattgg
     5761 gccaacgcga tttggaacaa ggccacgccc aaggagcgcg agttgctgga acagatcgtc
     5821 aatcggcccg cgaactagat ccgaccgcct accttgaggc gagcggctac accgtgaagc
     5881 gcgaagggcg acacctgtcc gtcaaggctg gcggtgatga ggcgtaccgc gtgacccggc
     5941 agcaggacgg gcgctggctc tggtgcgacc gctacggcaa cgacggcggg gacaatatcg
     6001 accttgtgcg cgagatcgaa cctggcaccg gctacgccga ggccgtctat cggctttcag
     6061 gtgcgccgac agtccggcag caaccgcgcc cgagcgagcc gaaacgccaa ccgccgcagc
     6121 taccggcgca agggctggca gcccgcgagc atggccgcga ctacctcaag ggccggggca
     6181 tcagcccgga caccatcgag cacgccgaga aggcgggcat ggtgcgctat gcagacggtg
     6241 gcgtgctgtt cgtcggctac gaccgtacag gcaccgcgca gaacgccaca cgccgcgcca
     6301 ttgcccccgc tgatccggtg cagaagcgcg acttacgcgg cagcgacaag atctatccgc
     6361 cgatcctgcc gggcgacccg gcaaaggtct ggatcgtgga aggtggcccg gatgcgctag
     6421 ccctgcacga catcgccaag cgcagcggcc agcagccgcc caccgtcatc gtgtcaggcg
     6481 gcgcgaacgt gcgcagcttc ttggagcgtg acgacgtgca agcgatcctg aagcgggccg
     6541 agcgcgtcac tgtggccggg gaaaacgaaa agaaccccga ggcgcaggcc aaggccgacg
     6601 ccgggcacca gaaacaggcg cagcgggtgg ccgaaatcac cgggcgcgag gtgcgccaat
     6661 ggacgccgaa gcccgagcat ggcaaagact tggccgacat gaacgcccgg caggtggcag
     6721 agatcgagcg caagcgacag gccgagatcg aggctgagag agcgcgaaat cgagagcttt
     6781 cacgcaagag ccgggggtat gatggcccca gcttcggcag ataagagcgc ctgtcagtcg
     6841 gtgtgatgaa ccgaccgaca ggctaaccat catcaacctg agtaggaggc cgatatggct
     6901 atcaacatta tggaggcgtt ccagcaggaa ccgccacaac tggacttcat ctggcccggc
     6961 tttctggctg gcaccgtggg cgcactggtg gcccccggcg cgaccggcaa gagcttctgg
     7021 gcgctggaag cggctatgtc catcgcttgc agcgtggcag gcggtgacgt ggtgaacctc
     7081 aagccgcagc acaccgggcg cgtggtctat ctggccggtg aagatcccga acccgccctg
     7141 atccggcgcg ttcacgccat cggccagcac ctcaaccagt cagcccgcga agccatcgcc
     7201 gagaacctga cccttgagcc gatcatgggc aagcgcctga acgtcatgga cgaggagcac
     7261 ttgcgccgcg tcatcgagta cagcgccggg gcgcggctga tcgtgctgga caccctgagc
     7321 cggattcaca gcctcgacga gaacagcaac ggcgacatgg cgcggcttgt cgccacgctg
     7381 gaacacgtcg cggccagcac tggcgcgtct gtcctgtacc tgcaccacgt cagcaagggc
     7441 agcgcccgcg aggggcaaac cgaccagcag caggcagcgc ggggcgcatc ggcactgatc
     7501 gacaacgccc gctggtgcgg cttcgtcgcc aagatgaccg aggacgaggc caagcgcctg
     7561 agcgaccgcg cctatgaccg tcagcccatc ggcaacgacc ggcgcggctt cttcgtgcgc
     7621 ttcggcgtga gcaaacagaa ctacgacgcc accccgcttg accagtggta tgagcgccga
     7681 gacggcggcg tgctggttcc ggtggaactg ctggaagtgc cgaacggcaa gaaggggggc
     7741 agccgtg
//
