LOCUS       NZ_KT693145             4130 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Escherichia coli strain E35BA plasmid pE35BA_1, complete sequence.
ACCESSION   NZ_KT693145
VERSION     NZ_KT693145.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226387
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Plasmid flux in Escherichia coli ST131 sublineages, analyzed by
            plasmid constellation network (PLACNET), a new method for plasmid
            reconstruction from whole genome sequences
  JOURNAL   PLoS Genet 10 (12), e1004766 (2014)
   PUBMED   25522143
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Baria,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2015) Instituto de Biomedicina y Biotecnologia de
            Cantabria, Universidad de Cantabria, C/ Albert Einstein No. 22,
            PCTCAN, Santander, Cantabria 39011, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Velvet v. VelvetOptimiser.pl
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009966785.1-RS_2024_05_18
            Annotation Date              :: 05/18/2024 01:27:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KT693145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E35BA"
                     /isolation_source="ESBL clinical isolate"
                     /db_xref="taxon:562"
                     /plasmid="pE35BA_1"
                     /subtype="ST131"
     gene            complement(394..648)
                     /gene="mobC"
                     /locus_tag="HTG77_RS00005"
     CDS             complement(394..648)
                     /gene="mobC"
                     /locus_tag="HTG77_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            827..2986
                     /gene="mobQ"
                     /locus_tag="HTG77_RS00010"
     CDS             827..2986
                     /gene="mobQ"
                     /locus_tag="HTG77_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3244..3936)
                     /locus_tag="HTG77_RS00015"
     CDS             complement(3244..3936)
                     /locus_tag="HTG77_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(650..826)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          827..1185
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(3245..3936)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          741..2983
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacaccttaa attcctccac ctttacctta aattcctcca catataccac tataacatat
       61 tgattttatt aatgaatttc acccctaaaa caagtattaa aaacaagatc ttaaaaacaa
      121 gatctaaaaa cttaacggcg agcctgtgga taacttgggg tgctgaaaac aaaaaaccaa
      181 taaaaacaat gtgttgcaag aaagcagaat tgcaagttcc gaatactccc cttccaaccc
      241 tgtggaaatc cagtgttcat gcgagttcag cgcatatcac ctgccgaaaa accgtgcgta
      301 tgtgctcaac ctggcgacgg attcacttcc gcttcctcgc ccactcgctc gctaacgctc
      361 ggtcgctggg gctgcggcga gcggacgata caatcactgt cctggcagcg cctccagctc
      421 gttaatcagc ggcagtatgc gcgtcacatc agcctgtcgt gtcacctctg ccttcaggaa
      481 ctggagtaac caggcacgca actgcacctc ctccggcttt ctgacctttg ccagcaatgc
      541 tgcacccaca acaaccttct gcgctgtgtc caggctccgg ctcttcgcct tcaggcgctg
      601 taatctcgcc tcagcatcag caatcttctg ttcgagcgtt ctgctcatga tgtaactccg
      661 tgcgcgatga aaaatcgcat tttagcgcgt cactggtagt ttaaaaactg agctggcata
      721 atgcacggca aatcacgaag tgcgcactta tacaatctcc actccgtttc gattgtgtgc
      781 gccctgcggg gctgaaagaa aactacaaaa aggcattacg gcagaaatgg cgatttatca
      841 tctcagcatg aaaattatct ccagaagcag cggctacagc gccgtagctt ccgctgccta
      901 tcgttccggt tcactaatgc ttgatgagcg taccggactg actcacgact acacccgcaa
      961 aagcggtgtg gctgaagctg tgattctgac ccccgcgaca gcccctgcct ggtgtacgaa
     1021 ccgtgccgaa ctctggaatg ccgttgagaa ggccgaacgc cggaagaact cacaacttgc
     1081 gcgggaaatt gaactcgcta tcccccgtga actgccgcag gatgccgcac gggaaaccgt
     1141 tctcgccttt gtcagagaaa actttgtcag tcagggcatg attgctgatg tggcgttcca
     1201 tcacatggat aaaacaaatc cccatgcgca catcatgctg accacgaggg ctgtcggacc
     1261 tgctgggttc ggcggaaagg tcagggactg gaacgaccgg acacatgccg agacgtggcg
     1321 tgcatcatgg gctgaccatg caaacagagc actggcgaac gccggttatc aggaagagat
     1381 agaccatcgt tcatacgagc gacaggggct tgagaaaacg cctggtattc acctcggaaa
     1441 gtctgcctgt gcgatggaaa cacgcggtat cgaaacagaa cgcggcgaac agaatcgtct
     1501 gattaacagg cttaacctgg aaatacagat ttcccgcact gaactcagga acagcgggct
     1561 gacagagcct gcccgccgtg tggctgacct tctgaatatc gtgcttcctg acaacgcaac
     1621 ttcgtacaca ctacgtgaca tcatcgaagc gttgccgaag gacagtaatg cggcatggaa
     1681 actcaccagt aatgccatgt cgatgatggc agatatgcag gcgacgcgca gacgctggga
     1741 agagctgaac accgaacgaa aggaagcgat gagccatgct gccagcctga aaaaatcatc
     1801 gccgttttcg tccggttttt cccgtattcc cctgatgcaa tgggcggcac cggaataccg
     1861 tagcgaacag ggaaaaatcc agtcgctgag ccagcaaatg gaaaaattgc gacagcatta
     1921 ccggaaggtg gagaaacagg acattcccgc gagtcagacg gcatttgaaa aacagtggca
     1981 gcagtggggg gcatccggcc ttgcagctct cagagtccag ttgcttaaac gtgaagagga
     2041 attgcgccgg aaggagcagg aggatacaga acgtagtcgc gcggagcgac gagcggaaca
     2101 gctcaggaaa aatgacaatg ccgttatgga ttcaggggta ctgagcgcag tcgttacggg
     2161 gtatggcgag gcccccatgc ctggtaacgg ggaaatgacc tgttaccttc tgcttcacaa
     2221 ccggaacgga gaatacacgc tctgggggaa cgagctggag aaataccgca ccagaatctt
     2281 tgagcgtgtt gatttaatgc gtgacaggag tggttatatc tgtgaccgaa gcgaaatcgg
     2341 tcaacatcca ccactgcaac gtgtttattc ctctgcgacc tttgagcaac ttctgacgca
     2401 ggtttgccag acatggccgc aatatacccg caatctccgc cagccgaaga catggccgga
     2461 gtctttctgt cttggagaag accgccagcc agccatgcca tcactggcag caaggaaggt
     2521 ggattttacg cagggacgtc tgccaccgac gctgatgccg gtaatgagtt ctgtcgaccg
     2581 ggaaacccgc cagcttcagc ttttgctggt catgggcgtg gatgactctc tgggcggtgt
     2641 tgtccggctt aatggtacgc tgtatcccgc gtttgccgtt ccctcagccg ataacagcca
     2701 gctggtcatc agtgcactga ccgataaggg gttacggtat gcagggtatg gtgttgccgt
     2761 aaatcacgat gcagacagcc acatatcacc tgcgccggaa cttatggagt ttcatctgaa
     2821 aacccgtgag gcaccgcttt ttgctgccgt caacacacca gaaaaacagc cggatcatct
     2881 gttccgctcg ctgggtttca atcgtacctg ggacgaatgg cggcgcgagg aagatgcaag
     2941 aacacacacc accgagagga gacatgatcg cggttggtca cagtgaatgt gaacaatatt
     3001 ttgttttgtt taacatcgtg ttatgttcgt cgatgttctt cgatgttcat cgacagggat
     3061 gaatgcaaga agcacactca cataatgcaa gcatagtttt gtttttccgc ccccaaaaat
     3121 tgggggttct tttttcgctg catcagagcg tttttgacgc aatttttcac ttcaatagta
     3181 ccttcgcact actaacacaa aaaaaacgca gcagggagcg ttttaggtgc cttaaaatcg
     3241 attttagatc gtcatcttga tctgtttgtc ttccttgaag cgaaaatgca gggctacaac
     3301 cgtgcgcccc tttttgatcg tctcaaccgt tacagccagg tcgctttttt tgttcagttc
     3361 gtcaacacag ggttttatta gtattttatt taagtctctg aattgtttgt atttattttc
     3421 aatccctaac attgaacgaa agtcattaag agcaataatt ctctctcccg tactgcggaa
     3481 ttgctggagg aactcgtata ttcttatact gtaactactt gataaacctg atatgttttt
     3541 tataacaacg cgcgtgaatt gccccttcag ttgcgtcaga taaggcatta ccgcgtcaga
     3601 aaaggttatt cgagccttgg cctcaccttt agcgtactgt gcccgatact ggatccagcg
     3661 gaactcctca cgcttctcgt cattcttgag aatgatcgac ctgtcccata aacgatcgat
     3721 cgcttcctgt aactgaacct cggcattttt ccgccccatg tcagggaatg aatcaaaata
     3781 ttccgcagcg gtaatggtca ttgtcttttg caattcagca tcagcaccag atttcaatcg
     3841 actgatacac agcaacagaa aacgttgttc ctgtagggtc aacttgtagc tggcctcaac
     3901 caaagcattc gacttgtagg caatcaagtc cgacatacaa agatcccgtt caaatgtggt
     3961 gatgctttaa tgtgccccac aaataagaaa tgtggaatgt ttttttttat atcctcacat
     4021 ttctgtgaat aacaccttaa attcctccac ctttacctta aattcctcca cctaaacacc
     4081 ttaaattcct ccacctttac cttaaattcc tccacatata ccactataac
//
