LOCUS       NZ_KT693144             4081 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Escherichia coli strain FV9873 plasmid pFV9873_1, complete sequence.
ACCESSION   NZ_KT693144
VERSION     NZ_KT693144.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226388
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Plasmid flux in Escherichia coli ST131 sublineages, analyzed by
            plasmid constellation network (PLACNET), a new method for plasmid
            reconstruction from whole genome sequences
  JOURNAL   PLoS Genet 10 (12), e1004766 (2014)
   PUBMED   25522143
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Baria,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2015) Instituto de Biomedicina y Biotecnologia de
            Cantabria, Universidad de Cantabria, C/ Albert Einstein No. 22,
            PCTCAN, Santander, Cantabria 39011, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Velvet v. VelvetOptimiser.pl
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009966775.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 01:36:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KT693144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FV9873"
                     /isolation_source="ESBL clinical isolate"
                     /db_xref="taxon:562"
                     /plasmid="pFV9873_1"
                     /subtype="ST131"
     gene            join(2389..4081,1..466)
                     /gene="mobQ"
                     /locus_tag="HTF88_RS00005"
                     /pseudo=""
     CDS             join(2389..4081,1..466)
                     /gene="mobQ"
                     /locus_tag="HTF88_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     gene            complement(722..1423)
                     /locus_tag="HTF88_RS00010"
     CDS             complement(722..1423)
                     /locus_tag="HTF88_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1956..2210)
                     /gene="mobC"
                     /locus_tag="HTF88_RS00015"
     CDS             complement(1956..2210)
                     /gene="mobC"
                     /locus_tag="HTF88_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2212..2388)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2389..2747
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(723..1423)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2303..4080
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.663"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggattttacg cagggacgtc tgctaccgac gctgatgccg gtaatgagtt ctgtcgaccg
       61 ggaaacccgc cagcttcagc ttttgctggt catgggcgtg gatgactctc tgggcggtgt
      121 tgtccggctt aatggtacgc tgtatcccgc gtttgccgtt ccctcagccg ataacagcca
      181 gctggtcatc agtgcactga ccgataaggg gttacggtat gcagggtatg gtgttgccgt
      241 aaatcacgat gcagacagcc acatatcacc tgcgccggaa cttatggagt ttcatctgaa
      301 aacccgtgag gcaccgcttt ttgctgccgt caacacacca gaaaaacagc cggatcatct
      361 gttccgctcg ctgggtttta atcgtacctg ggacgaatgg cggcgcgagg aagatgcacg
      421 aacacacgcc accgagagga gacatgatcg cggttggtca cagtgaatgt gaacaatatt
      481 ttgttttgtt taacatcgtg ttatgttcgt cgatgttctt cgatgttcat cgacagggat
      541 gaatgcaaga agcacacaca taatgcaagc atagttttgt ttttccgccc ccaaaaaatg
      601 ggggttcttt tttcgctgca ttagagcgct tttgacgcaa tttttcactt caatagtacc
      661 ttcgcactac taacacaaaa aaaacgcagc agggagcgtt ttaggtgcct taaaatcgat
      721 tttagatcgt catcttgatt tgtttgtctt ccttgaagcg aaaatgcagg gctacaaccg
      781 tgcgcccctt tttgatcgtc tcaaccgtta cagccaggtc gctttttttg ttcagttcgt
      841 caatgcaggg tcttaataat tgctggttaa gtgatttata tgtttgatat ttatgctcta
      901 tatcaagcat tgagcgaaaa tcatcgagag cgataatacg ttcgccagta ctgcggaact
      961 gctggaggag ttcgtatatc cttatactgt aacttcttga taatctagat atattcttga
     1021 ttacaactct agtaaattgc ccttgtagtt gtgtcaaata aggcattact gcgtcagaaa
     1081 atgttatttg tgctctggcc tcgcctttag cgtactgtgc ccgatactgg atccagcgga
     1141 actcctcacg tttctcgtca tccttgagaa tgattgacct gtcccacaaa cgatcaattg
     1201 cttcctgtaa ctgaacctcg gcatttttcc gccccatgtc agggaatgaa tcgaaatatt
     1261 ccgcagctgt aatggtcatt gtcttttgca attttggaga ttctgcatca gccccagact
     1321 tcaatcgacc gatgcaaagt aacaaaaatc gctgttcctg tagggtcaat ttataactgg
     1381 cttcaaccag cgcattggat ttataggcaa ttagatccga catagagcat ccctccccaa
     1441 aaggtaatga ataaaagata aacataccct caacgaaagg caatgccttt ttattttaat
     1501 atcccttttt ctgtgaatat taccttaaat catattgcct ttaccttaaa ttcccccacc
     1561 taaacacctt aaatcatatt acctttacct taaatcacat tacctatagc attgtaacat
     1621 tttgttttct ataaggaatt ttggcgctaa aacaagtatt aaaaacaaga tcttaaaaac
     1681 aagatctaaa aacttaacga cgagcctgtg gataacttgg agtgctgaaa acaaaaaacc
     1741 aataaaaaca atgcgttgca aaaaagcaga attgcaagtt ccgaatacac cccctccaac
     1801 cctgtggaaa tccagtattc atgcgggttc agcgcatatc acctcccgaa aaaccgtgcg
     1861 tatgtgctca atcaggcgac ggattcactt ccgcttcctc gcccactcgc tcgctaacgc
     1921 tcggtcgctg ggactgcggc gagcggacga tacaatcact gtcctggcag cgcttccagc
     1981 tcgttaatca gcggcagtat gcgcgtcaca tcagcctgtc gtgtcacctc tgccttcagg
     2041 aactggagta accaggcacg caactgcacc tcctccggct ttctgacctt tgccagcaat
     2101 gctgcaccca caatgacctt ctgcgctgtg tccagactcc tgctcttcgc cttcaggcgc
     2161 tgtaatctgg cctcagcatc agcaatcttc tgttcgagtg ttctgctcat gatgtaactc
     2221 cgtgcgcgat gaaaaatcgc attttagcgc gtcactggta gtttaaaaac tgagctggca
     2281 taatgcacgg aaaatcacga agtgcgcact tatacaatct ccactccgtt tcgattgtgt
     2341 gcgccctgcg gggctgaaag aaaaccacaa aaaggcatta cggcagaaat ggcgatttat
     2401 catctcagca tgaaaatcat ctccagaaac agcggctaca gcgccgttgc ttctgctgcc
     2461 tatcgttccg gttcactgat gcttgatgag cgtaccggac tgactcacga ctacacccgc
     2521 aaaagcggtg tggctgaagc tgtgattctg actcccgcga cagcccctgc ctggtgtacg
     2581 aaccgtgccg aactctggaa tgccgttgag aaggccgaac gccggaagaa ctcacaactt
     2641 gcgcgggaaa ttgaactcgc catcccccgc gaactaccgc aggatgccgc acgggaaacc
     2701 gttctcgcct ttgtcagaga aaactttgtc agtcagggca tgattgctga tgtggcgttc
     2761 catcacatgg ataaaacaaa tccccatgcg cacatcatgc tgaccacgag agctgtcgga
     2821 cctgctggat tcggtggaaa ggtcagggac tggaacgacc ggacacatgc cgagacgtgg
     2881 cgtgcatcat gggctgacca tgcgaacagg gcactggcga acgccggtta tcaggaagag
     2941 atagaccatc gttcatacga gcgacagggg cttgagaaaa cgcctggtat tcacctcgga
     3001 aagtctgcct gtgcgatgga aacacgcggt atcgaaacag aacgcggcga acagaatcgt
     3061 ctgattaaca gacttaacct ggaaatacag atttcccgta tcgaactcag aaacagcggg
     3121 ctgacagagc ctgcccgccg tgtggctgac cttctgaata tcgtgcttcc tgacaacgca
     3181 acttcgtaca cactacgtga catcatcgaa gcgttgccga aggacagtaa tgcggcatgg
     3241 aaactcacca gtaatgccat gtcgatgatg gcagatatgc aggcgacgcg cagacgctgg
     3301 gaagagctga acaccgaacg aaaggaagcg atgagccatg ctgccagcct gaaaaaatca
     3361 tcgccgtttt cgtccggttt ttcccgtatt cccctgatgc aatgggcggc accggaatac
     3421 cgtagcgaac agggaaaaat ccagtcgctg agccagcaaa tggaaaaatt gcgacagcat
     3481 taccggaagg tggagaaaca ggacattccc gcgagtcaga cggcatttga aaaacagtgg
     3541 cagcagtggg gggcatccgg ccttgcagct ctcagagtcc agttgcttaa acgtgaagag
     3601 gaattgcgcc ggaaggagca ggaggataca gaacgtagtc gcgcggagcg acgagcggaa
     3661 cagctcagga aaaatgacaa tgccgttatg gattcagggg tactgagcgc agtcgttacg
     3721 gggtatggcg aggcccccat gcctggtaac ggggaaatga cctgttacct tctgcttcac
     3781 aaccggaacg gagaatacac gctctggggg aacgagctgg agaaataccg caccagaatc
     3841 tttgagcgtg ttgatttaat gcgtgacagg agtggttata tctgtgaccg aagcgaaatc
     3901 ggtcaacatc caccactgca acgtgtttat tcctctgcga cctttgagca acttctgacg
     3961 caggtttgcc agacatggcc gcaatatacc cgcaatctcc gccagccgaa gacatggccg
     4021 gagtctttct gtcttggaga agaccgccag ccagccatgc catcactggc agcaaggaag
     4081 g
//
