LOCUS       NZ_KT693143             4082 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Escherichia coli strain E2022 plasmid pE2022_4, complete sequence.
ACCESSION   NZ_KT693143
VERSION     NZ_KT693143.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226389
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Plasmid flux in Escherichia coli ST131 sublineages, analyzed by
            plasmid constellation network (PLACNET), a new method for plasmid
            reconstruction from whole genome sequences
  JOURNAL   PLoS Genet 10 (12), e1004766 (2014)
   PUBMED   25522143
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Baria,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2015) Instituto de Biomedicina y Biotecnologia de
            Cantabria, Universidad de Cantabria, C/ Albert Einstein No. 22,
            PCTCAN, Santander, Cantabria 39011, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Velvet v. VelvetOptimiser.pl
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009966765.1-RS_2024_05_15
            Annotation Date              :: 05/15/2024 01:38:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KT693143.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2022"
                     /isolation_source="ESBL clinical isolate"
                     /db_xref="taxon:562"
                     /plasmid="pE2022_4"
                     /subtype="ST131"
     gene            95..349
                     /gene="mobC"
                     /locus_tag="HTG88_RS00005"
     CDS             95..349
                     /gene="mobC"
                     /locus_tag="HTG88_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            882..1583
                     /locus_tag="HTG88_RS00010"
     CDS             882..1583
                     /locus_tag="HTG88_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1839..3998)
                     /gene="mobQ"
                     /locus_tag="HTG88_RS00015"
     CDS             complement(1839..3998)
                     /gene="mobQ"
                     /locus_tag="HTG88_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          2..94
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.78e-41"
     Typing          complement(3641..3999)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4000..4082
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="100.0"
                     /evalue="4.69e-34"
     Typing          883..1583
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1843..4082)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cacttcgtga ttttccgtgc attatgccag ctcagttttt aaactaccag tgacgcgcta
       61 aaatgcgatt tttcatcgcg cacggagtta catcatgagc agaacactcg aacagaagat
      121 tgctgatgct gaggccagat tacagcgcct gaaggcgaag agcaggagtc tggacacagc
      181 gcagaaggtc attgtgggtg cagcattgct ggcaaaggtc agaaagccgg aggaggtgca
      241 gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg acacgacagg ctgatgtgac
      301 gcgcatactg ccgctgatta acgagctgga agcgctgcca ggacagtgat tgtatcgtcc
      361 gctcgccgca gtcccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga
      421 atccgtcgcc tgattgagca catacgcacg gtttttcggg aggtgatatg cgctgaaccc
      481 gcatgaatac tggatttcca cagggttgga gggggtgtat tcggaacttg caattctgct
      541 tttttgcaac gcattgtttt tattggtttt ttgttttcag cactccaagt tatccacagg
      601 ctcgtcgtta agtttttaga tcttgttttt aagatcttgt ttttaatact tgttttagcg
      661 ccaaaattcc ttatagaaaa caaaatgtta caatgctata ggtaatgtga tttaaggtaa
      721 aggtaatatg atttaaggtg tttaggtggg ggaatttaag gtaaaggcaa tatgatttaa
      781 ggtaatattc acagaaaaag ggatattaaa ataaaaaggc attgcctttc gttgagggta
      841 tgtttatctt ttattcatta ccttttgggg agggatgctc tatgtcggat ctaattgcct
      901 ataaatccaa tgcgctggtt gaagccagtt ataaattgac cctacaggaa cagcgatttt
      961 tgttactttg catcggtcga ttgaagtctg gggctgatgc agaatctcca aaattgcaaa
     1021 agacaatgac cattacagct gcggaatatt tcgattcatt ccctgacatg gggcggaaaa
     1081 atgccgaggt tcagttacag gaagcaattg atcgtttgtg ggacaggtca atcattctca
     1141 aggatgacga gaaacgtgag gagttccgct ggatccagta tcgggcacag tacgctaaag
     1201 gcgaggccag agcacaaata acattttctg acgcagtaat gccttatttg acacaactac
     1261 aagggcaatt tactagagtt gtaatcaaga atatatctag attatcaaga agttacagta
     1321 taaggatata cgaactcctc cagcagttcc gcagtactgg cgaacgtatt atcgctctcg
     1381 atgattttcg ctcaatgctt gatatagagc ataaatatca aacatataaa tcacttaacc
     1441 agcaattatt aagaccctgc attgacgaac tgaacaaaaa aagcgacctg gctgtaacgg
     1501 ttgagacgat caaaaagggg cgcacggttg tagccctgca ttttcgcttc aaggaagaca
     1561 aacaaatcaa gatgacgatc taaaatcgat tttaaggcac ctaaaacgct ccctgctgcg
     1621 ttttttttgt gttagtagtg cgaaggtact attgaagtga aaaattgcgt caaaagcgct
     1681 ctaatgcagc gaaaaaagaa cccccatttt ttgggggcgg aaaaacaaaa ctatgcttgc
     1741 attatgtgtg tgcttcttgc attcatccct gtcgatgaac atcgaagaac atcgacgaac
     1801 ataacacgat gttaaacaaa acaaaatatt gttcacattc actgtgacca accgcgatca
     1861 tgtctcctct cggtggcgtg tgttcgtgca tcttcctcgc gccgccattc gtcccaggta
     1921 cgattaaaac ccagcgagcg gaacagatga tccggctgtt tttctggtgt gttgacggca
     1981 gcaaaaagcg gtgcctcacg ggttttcaga tgaaactcca taagttccgg cgcaggtgat
     2041 atgtggctgt ctgcatcgtg atttacggca acaccatacc ctgcataccg taacccctta
     2101 tcggtcagtg cactgatgac cagctggctg ttatcggctg agggaacggc aaacgcggga
     2161 tacagcgtac cattaagccg gacaacaccg cccagagagt catccacgcc catgaccagc
     2221 aaaagctgaa gctggcgggt ttcccggtcg acagaactca ttaccggcat cagcgtcggt
     2281 agcagacgtc cctgcgtaaa atccaccttc cttgctgcca gtgatggcat ggctggctgg
     2341 cggtcttctc caagacagaa agactccggc catgtcttcg gctggcggag attgcgggta
     2401 tattgcggcc atgtctggca aacctgcgtc agaagttgct caaaggtcgc agaggaataa
     2461 acacgttgca gtggtggatg ttgaccgatt tcgcttcggt cacagatata accactcctg
     2521 tcacgcatta aatcaacacg ctcaaagatt ctggtgcggt atttctccag ctcgttcccc
     2581 cagagcgtgt attctccgtt ccggttgtga agcagaaggt aacaggtcat ttccccgtta
     2641 ccaggcatgg gggcctcgcc ataccccgta acgactgcgc tcagtacccc tgaatccata
     2701 acggcattgt catttttcct gagctgttcc gctcgtcgct ccgcgcgact acgttctgta
     2761 tcctcctgct ccttccggcg caattcctct tcacgtttaa gcaactggac tctgagagct
     2821 gcaaggccgg atgcccccca ctgctgccac tgtttttcaa atgccgtctg actcgcggga
     2881 atgtcctgtt tctccacctt ccggtaatgc tgtcgcaatt tttccatttg ctggctcagc
     2941 gactggattt ttccctgttc gctacggtat tccggtgccg cccattgcat caggggaata
     3001 cgggaaaaac cggacgaaaa cggcgatgat tttttcaggc tggcagcatg gctcatcgct
     3061 tcctttcgtt cggtgttcag ctcttcccag cgtctgcgcg tcgcctgcat atctgccatc
     3121 atcgacatgg cattactggt gagtttccat gccgcattac tgtccttcgg caacgcttcg
     3181 atgatgtcac gtagtgtgta cgaagttgcg ttgtcaggaa gcacgatatt cagaaggtca
     3241 gccacacggc gggcaggctc tgtcagcccg ctgtttctga gttcggtacg ggaaatctgt
     3301 atttccaggt taagtctgtt aatcagacga ttctgttcgc cgcgttctgt ttcgataccg
     3361 cgtgtttcca tcgcacaggc agactttccg aggtgaatac caggcgtttt ctcaagcccc
     3421 tgtcgctcgt atgaacgatg gtctatctct tcctgataac cggcgttcgc cagtgccctg
     3481 ttcgcatggt cagcccatga tgcacgccac gtctcggcat gtgtccggtc gttccagtcc
     3541 ctgacctttc caccgaatcc agcaggtccg acagctctcg tggtcagcat gatgtgcgca
     3601 tggggatttg ttttatccat gtgatggaac gccacatcag caatcatgcc ctgactgaca
     3661 aagttttctc tgacaaaggc gagaacggtt tcccgtgcgg catcctgcgg tagttcgcgg
     3721 gggatggcga gttcaatttc ccgcgcaagt tgtgagttct tccggcgttc ggccttctca
     3781 acggcattcc agagttcggc acggttcgta caccaggcag gggctgtcgc gggagtcaga
     3841 atcacagctt cagccacacc gcttttgcgg gtgtagtcgt gagtcagtcc ggtacgctca
     3901 tcaagcatca gtgaaccgga acgataggca gcagaagcaa cggcgctgta gccgctgttt
     3961 ctggagatga ttttcatgct gagatgataa atcgccattt ctgccgtaat gcctttttgt
     4021 ggttttcttt cagccccgca gggcgcacac aatcgaaaca gagtggagat tgtataagtg
     4081 cg
//
