LOCUS       NZ_KT074362             4661 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain GDP53 plasmid pSC137, complete sequence.
ACCESSION   NZ_KT074362
VERSION     NZ_KT074362.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226652
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Fang,L., Sun,J. and Liu,Y.
  TITLE     Transfer of a Chromosome-encoded BlaCMY-2 Gene in Three E. coli
            ST641Isolates through Genetic Re-arrangements with a ColE1-like
            Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Fang,L., Sun,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2015) College of Veterinary Medicine, South China
            Agricultural University, Wushan Road, Guangzhou, Guangdong 510642,
            China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SeqMan v. v. 7.1
            Sequencing Technology             :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009966065.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 01:58:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 3
            CDSs (with protein)               :: 3
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KT074362.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GDP53"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pSC137"
     gene            complement(<111..242)
                     /locus_tag="HTG20_RS00025"
                     /pseudo=""
     CDS             complement(<111..242)
                     /locus_tag="HTG20_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /name="zinc-ribbon domain-containing protein"
     gene            complement(550..663)
                     /locus_tag="HTG20_RS00030"
     CDS             complement(550..663)
                     /locus_tag="HTG20_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071778980.1"
                     /translation="MHEPPVQSDRCVFSGNYRLESKPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1848..>2030)
                     /locus_tag="HTG20_RS00005"
                     /pseudo=""
     CDS             complement(1848..>2030)
                     /locus_tag="HTG20_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /name="NINE protein"
     gene            <2027..2269
                     /locus_tag="HTG20_RS00010"
                     /pseudo=""
     CDS             <2027..2269
                     /locus_tag="HTG20_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003862821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /name="cupin domain-containing protein"
     gene            complement(2307..3449)
                     /gene="wzz(fepE)"
                     /locus_tag="HTG20_RS00015"
     CDS             complement(2307..3449)
                     /gene="wzz(fepE)"
                     /locus_tag="HTG20_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435173.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_021544858.1"
                     /translation="MSAMDFKKNTDLDFPRYATPAVSTKEIDLLGLLDVLLAAKKRIVT
                     IVLAFAVVGLAIAFLIPQKWTSKAVITPAEQTQWNTLRQMMVTLQVLDVDAKVSRGDVF
                     NLFIKKFQSQSLLEEYMKTSPYVMSQLEGTEVDPLELHRAVVNIAEKMKATDNTQVKDA
                     DKAPYTSWTLSFTAPTAGDAQTVLEGYINYISRIVEQETMENIRNQITLKTKVVQQQLE
                     LDRVRLTNIHNTNLQRLNYSLEVANAAGIKKPVYSNGQAVKDDPDYSVALGADGIAQKL
                     QIEKNLKDVAELNADFQNREYYLTQLQKLSVEDVKLEPFRYQLSPSLPVKKDGPGKALI
                     VLLAGILGGLFACGSVLLREAMLSRNPLPEPITAVTPATE"
                     /name="wzz(fepE)"
     gene            3644..4624
                     /locus_tag="HTG20_RS00020"
     CDS             3644..4624
                     /locus_tag="HTG20_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_041206478.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAIEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     Typing          complement(547..932)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.373"
                     /coverage="90.0"
                     /evalue="1.04e-173"
ORIGIN
        1 ctaaataggc tgattcaagg catttacggg agaaaaaatc ggctcaaaca tgaagaaatg
       61 aaatgactga gtcaaccgag aagaatttcc ccgcttattc gcaccttccc taatagcgcg
      121 gctgcgattc tacttttttc acctgaacct gaagaggcgt ttgtggctcc gcagtgtggg
      181 catgactttg ctgtttcgtg tatttccttg ccgcatcctc ggcagtaaac cattgaactc
      241 attttttatc ctcgagctta tttttagttg ggatgcataa tatccgatgt tttataacga
      301 tatgcatcct atatgctcgg gtgggataga tttccattat ggtgaaggat cttcttgaga
      361 tcctttctgt agcgtcgtaa tctcttgctc agtaaacgaa aaaaccgccc agcaaggcgg
      421 tttttcgaag gttctcagag ctgcaactct ttgaaccgtg gtaactggct tatgaggagc
      481 gcagtaacca aatctgttct ttcagtttag ccttaacagg cgcataactt caagactaac
      541 tcctctaaat cagttaccag tggctgctgc cagtggcgct ttgtcgtgtc tttccggctt
      601 ggactcaagt cgatagttac cggagaagac gcagcggtcg gactgaacgg ggggttcgtg
      661 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
      721 ataaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
      781 gcacgaggga gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca
      841 ccactgattt gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc tatggaaaac
      901 cggcaacgcc gcggcctttt cttttgcctc tcgatcctct ctcattgcct gtgccttaat
      961 gtctccgttc cctccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     1021 gaagcggaat atccccggac ttatcttcgg caccgtacgc gccagtgctg cattttttaa
     1081 tacgatgcag gatagggcta acgccgcaaa gtgacgtcac ctgacgttcc tggattacaa
     1141 aggttaaagc agcggacagg aaatgttttg tgcccacagc tatgctggct tattcacaaa
     1201 acagcgggac aagatgtgtt tttggagtac cgccggcccg ctaaagactc ctggttagct
     1261 cccgcatgtt tgctatcttt ttgctaccat tttgctactt ttttgctatc tccagagtct
     1321 tctggtcttt cggttgctat ccttttgcta cttttttgct atcaaaatgc tacctctccc
     1381 ttcttgcaat aaatgaccaa ggcacgttag cgatgtatac atcgccgcgc cgcaagggtg
     1441 ccccttccga atcccgttca tcactgcgtg ctgattcatc cacgttaaat ccgaccccct
     1501 tagcaataaa tccagccatg cctgacggcc tggccaacgc ctcacggccg cacagtaaac
     1561 tgcacacccg tgaggcgtta ttcgtgaggt tcagtggtgc ttttgggcgt ccagccggat
     1621 tttagccgct aaaatcccgg ccatgcatgt gtgtgcatag ccatgcagac gcgccagaat
     1681 catcaggcgc gttttttcac gatataggta cagggaagtg tcggaaacag gcaggaagac
     1741 ccagaaacgc cctgagagcc tctcaggcgg tgcacgcaac ggatgttatg gtaaatctgc
     1801 ctcacagact ggtacaaaaa aagcccgaag ggcttttttg ctatgagtta gccatatttt
     1861 cttgcgaatt tttcatcgga atcgcataga taaataataa actcaataaa cgcgatgatc
     1921 gcgggtatga atgtccagca aaaaattaga tacaggaatc cctgtcctat tttacctaag
     1981 taaaatttat gtacgccaaa tccaccaagg aagaatgcta agaaagatga tggcggtgga
     2041 ggtcaatttt gagcagggtg cgattggccc gatgcacaat cacccgcatg aacagcttac
     2101 ctatgtgctc tccggggagt tcgagtttac gataggtgaa gaaaagcaga tcgtgacggc
     2161 gggcgatacg ctctataaag cgccgcacgt gatgcacggc tgcatttgcc tgaagcccgg
     2221 cacccttctg gataccttta cgccggtgcg cgacgatttc ctgaaataaa aaaaggcgct
     2281 gaatcagcgc ctttttaata tcgtcgttac tctgttgctg gcgttacggc cgttatcggc
     2341 tctggcaacg gattacgcga gagcattgcc tcacgcagca gcacgctgcc gcaggcaaac
     2401 aggccgccca gaatccctgc cagcagcaca atcagggctt tgcccggacc gtcttttttc
     2461 accggcagtg acggtgaaag ctgatacctg aagggttcaa gcttcacgtc ttccacggac
     2521 agtttttgca actgagtcag atagtattca cggttctgga aatccgcatt cagctcggcc
     2581 acgtccttca gattcttttc aatctgcagc ttttgcgcaa taccgtcagc acccagcgcc
     2641 acggagtagt caggatcgtc ttttaccgcc tgtccgttgc tgtaaaccgg cttcttaatg
     2701 cctgccgcgt tcgccacttc cagcgaatag ttgaggcgct gcaggttggt gttgtggata
     2761 ttggtcagac gcacgcgatc cagctccagc tgctgctgaa cgactttcgt tttcagcgtg
     2821 atctggttac ggatattttc catcgtctcc tgctcaacaa tgcgggagat atagttgatg
     2881 taaccttcca gaaccgtctg cgcgtctccc gccgtcgggg cggtaaagct cagcgtccat
     2941 gaggtatacg gcgctttgtc tgcgtcttta acctgcgtgt tatctgtcgc tttcattttc
     3001 tcagcaatat tcaccaccgc gcgatgcagc tccagcgggt ctacctcggt gccttccagc
     3061 tgagacatca cgtacgggga ggttttcatg tactcttcga gcagcgactg cgactgaaac
     3121 tttttgatga acaggttaaa cacgtctcca cggctcacct tcgcgtcgac gtccagcacc
     3181 tgcagcgtca ccatcatctg gcgcagcgtg ttccactgcg tctgctcggc cggcgtgatc
     3241 acggctttac tggtccattt ttgcgggatt aaaaacgcaa tggccaaccc tactacggcg
     3301 aacgccagga cgatcgtcac aatacgtttt ttggctgcca gaagtacgtc taacaacccc
     3361 aggagatcta tctctttggt gcttaccgcc ggagtggcat aacggggaaa atcgaggtcc
     3421 gtgtttttct tgaaatccat cgctgacata accgttcttc tttttggagc gcagtaaatt
     3481 tctgcctgat tggttgcagt ggtcggatga tacaagaaag taagaataat ctgaacgcca
     3541 gattgatcta tctgtagacc gactatcaga tttagggaag gtgcgaacaa gtccctgata
     3601 tgagatcatg tttgtcatct ggagccatgg aacagggttc atcatgagtc atcaacttac
     3661 cttcgccgac agtgaattca gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc
     3721 ccgcatggag cagattctgc catggcaaaa catggtggaa gtcatcgagc cgttttaccc
     3781 caaggctggt aatggccggc gaccttatcc gctggaaacc atgctacgca ttcactgcat
     3841 gcagcattgg tacaacctga gcgatggcgc gatcgaagat gctctgtacg aaatcgcctc
     3901 catgcgtctg tttgcccggt tatccctgga tagcgccttg ccggaccgca ccaccatcat
     3961 gaatttccgc cacctgctgg agcagcatca actggcccgc caattgttca agaccatcaa
     4021 tcgctggctg gccgaagcag gcgtcatgat gactcaaggc accttggtcg atgccaccat
     4081 cattgaggca cccagctcga ccaagaacaa agagcagcaa cgcgatccgg agatgcatca
     4141 gactaagaaa ggcaatcagt ggcactttgg catgaaggcc cacattggtg tcgatgccaa
     4201 gagtggcctg acccacagcc tagtcaccac cgcggccaac gagcatgacc tcaatcagct
     4261 gggtaatctg ctgcatggag aggagcaatt tgtctcagcc gatgccggct accaaggggc
     4321 gccacagcgc gaggagctgg ccgaggtgga tgtggactgg ctgatcgccg agcgccccgg
     4381 caaggtaaga accttgaaac agcatccacg caagaacaaa acggccatca acatcgaata
     4441 catgaaagcc agcatccggg ccaaggtgga gcacccattt cgcatcatca agcgacagtt
     4501 cggcttcgtg aaagccagat acaaggggtt gctgaaaaac gataaccaac tggcgatgtt
     4561 attcacgctg gccaacctgt ttcgggcgga ccaaatgata cgtcagtggg agagatctca
     4621 ctaaaaactg ggaataacgc cttaaatggc gaagaaacgg t
//
