LOCUS       NZ_KM276081             4229 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Staphylococcus hyicus strain 1211 plasmid pSWS1211, complete
            sequence.
ACCESSION   NZ_KM276081
VERSION     NZ_KM276081.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226862
KEYWORDS    RefSeq.
SOURCE      Staphylococcus hyicus
  ORGANISM  Staphylococcus hyicus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Wendlandt,S., Kadlec,K. and Schwarz,S.
  TITLE     Four novel plasmids from Staphylococcus hyicus and CoNS that carry a
            variant of the spectinomycin resistance gene spd
  JOURNAL   J Antimicrob Chemother 70 (3), 948-949 (2015)
   PUBMED   25389224
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Wendlandt,S., Kadlec,K. and Schwarz,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2014) Institute of Farm Animal Genetics,
            Friedrich-Loeffler-Institut, Hoeltystrasse 10, Neustadt-Mariensee
            31535, Germany
COMMENT     ##Assembly-Data-START##
            Sequencing Technology             :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009963865.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:50:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4
            CDSs (total)                      :: 4
            Genes (coding)                    :: 3
            CDSs (with protein)               :: 3
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM276081.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Staphylococcus hyicus"
                     /mol_type="genomic DNA"
                     /strain="1211"
                     /db_xref="taxon:1284"
                     /plasmid="pSWS1211"
     gene            87..1031
                     /locus_tag="HTD16_RS00005"
     CDS             87..1031
                     /locus_tag="HTD16_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722130.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_107511000.1"
                     /translation="MSKKEQEIYSKQVTSGEVQNYSLTGYSNSRLSVQTLGQSQPKLSF
                     DAMTIVGNLNKNNAQKLSEFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKSVK
                     KTIFYGRNGKPETKYFGVRDSDKFIRIYNKKQERKDNADIEVVSEHLWRVEIELKRDMV
                     DYWNDCFNDLHILKPAWTTLEKINEQAMVYTLLHEESMWGKLSRNTKYKFKNLIKEISP
                     VDLTDLMKLTLKENEKQLQKQIDFWQREFRFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1057..1818
                     /gene="ant(9)"
                     /locus_tag="HTD16_RS00010"
     CDS             1057..1818
                     /gene="ant(9)"
                     /locus_tag="HTD16_RS00010"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021285909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)"
                     /protein_id="WP_031901094.1"
                     /translation="MEEPNKQIDNVLIELKRLFSKDLLGVYLYGSYVKGGLKKDSDVDF
                     LVIINRDMTKEEKRILISKIMPISKEIGEDTSLKYIELTVLNYHENENWSYPPIEEFIY
                     GEWLREDYLNYFIPEKNNNIDLTILLYQAKLSSISIYGENNINNLIPDVPFIDLQKAIK
                     ESSKELIKDFYGDETNVILTLCRMIVTYETGKFYSKDLAGSMIIENLSIEENNLISLAI
                     SSYKNGNSVDWELFPVKSVIKKLYAYLNYKL"
                     /name="ant(9)"
     gene            2134..3344
                     /gene="mobV"
                     /locus_tag="HTD16_RS00015"
                     /pseudo=""
     CDS             2134..3344
                     /gene="mobV"
                     /locus_tag="HTD16_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002493196.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            3904..4068
                     /locus_tag="HTD16_RS00020"
     CDS             3904..4068
                     /locus_tag="HTD16_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_107510999.1"
                     /translation="MVRTKVGITLTEDTLKRLEEIAEQMGLTKSQAISMLVNKEYLSKY
                     QDKDKVFKA"
                     /name="CopG family transcriptional regulator"
     ARG             1058..1815
                     /name="spd"
                     /gene="spd"
                     /gene_name="ANT(9) family aminoglycoside
                     nucleotidyltransferase Spd"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.44"
                     /coverage="100.0"
     Typing          88..1031
                     /name="rep_cluster_1078"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.894"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2135..3174
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.695"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctagatatta aacgatttaa gtttattctt caagatatat atttgggtga gcgacttctt
       61 aaacaaaatt aaggagtcga ttttttatga gtaaaaaaga acaagaaatc tattcaaaac
      121 aagttacatc aggggaagtg caaaattact ccttaaccgg ctactctaat agccggttaa
      181 gtgttcaaac tttgggacaa tctcaaccaa aattaagttt tgatgctatg acaattgttg
      241 gaaatcttaa taaaaacaat gctcagaaac tatctgaatt tatgagtgtt gagccacaaa
      301 ttagactttg ggatatacta caaacgaaat ttaaagctaa agctctacaa gaaaaagttt
      361 atattgaata tgacaaagta aaagcagata cgtgggacag acgtaatatg cgcgttgaat
      421 ttaatccgaa taaacttaca catgaagaaa tgctttggtt aaaacaaaat attattgatt
      481 acatggaaga tgacggtttt acaagattag acttagcttt tgattttgaa gatgatttga
      541 gcgactacta tgcaatgact gataaatcgg ttaagaaaac tatcttttat ggtcgtaacg
      601 gtaaaccaga aacgaaatat tttggtgtcc gtgatagtga taaatttatt agaatttata
      661 ataaaaaaca ggaacgtaag gataatgcag atattgaagt tgtttcagaa catttatggc
      721 gtgtggaaat tgaattaaaa agagatatgg ttgattattg gaacgattgt tttaatgatt
      781 tacatatttt gaaacctgct tggactactt tagaaaaaat taatgagcaa gctatggttt
      841 atactttgtt acatgaagaa agtatgtggg ggaaattaag taggaatact aagtataaat
      901 ttaaaaattt aattaaagaa atatctccag ttgatttaac tgatttaatg aaattgactt
      961 taaaagagaa cgaaaaacaa ttgcaaaagc agattgattt ttggcaacgt gaatttagat
     1021 tttggaaata atatttaaag atgagaggag tcaataatgg aggaaccaaa taagcaaatt
     1081 gataatgttt taatagaatt gaaacgattg ttttcaaaag atttattagg agtctattta
     1141 tatggttctt atgttaaagg gggtttaaaa aaagatagtg atgttgattt tctagtgata
     1201 ataaatagag atatgactaa agaagaaaaa agaatattaa tttcgaaaat tatgcctatc
     1261 tctaaagaaa ttggtgaaga tacaagttta aaatatatag aattaactgt gcttaattat
     1321 catgaaaatg aaaattggtc ttatccacct attgaagagt ttatctatgg ggaatggctt
     1381 agagaagatt atttaaatta ttttattcca gaaaagaata acaacattga tttaacaata
     1441 ttattatatc aagccaagct ttcttcaata tcaatttatg gcgaaaataa tattaataac
     1501 ttaattcctg atgttccatt tattgattta caaaaagcta taaaggaaag ttctaaagaa
     1561 ttgataaaag atttttatgg tgatgaaaca aatgttattt taaccctttg tcgtatgatc
     1621 gtaacttatg aaacaggtaa gttttattca aaagatttag ctggcagtat gataatagaa
     1681 aatttatcaa ttgaagaaaa taatttaata agtttagcta tttctagtta taaaaatggt
     1741 aatagcgttg attgggaact ttttcctgtt aagagtgtca ttaaaaaact ttatgcttat
     1801 ttgaattata aattatgata atagcttttg tgatatttgc aatatcaagt attccttaaa
     1861 ttttgaaaaa gaaagtaagt agttgaatac atgttgtttt agttttgatc tttatattta
     1921 acaaccacta ttttggttgc tggtgttttg attttgaatt tggttttgaa cttatggact
     1981 gacttgttca gtccattttt tgtgcttgca caaaaacatg ttttagcctc gcagagcata
     2041 cggtttaatg acttattgta aaataagtct agtatgttag acttaaagta ttaaattacc
     2101 taactaacct tattgttgag gagtgaatta tatatgtctt attcaattat tagagtttca
     2161 aaagtcaaaa caggaacaaa tacaacaggc atacaaaaac atgttcaaag agaaaataat
     2221 aattatgaaa atgaagatat agaccatagt aaaacttact taaattatga tttggtaaat
     2281 gctaataaac agaattttaa taacttgatt gatgaaaaaa ttgaacagaa ttatacaggt
     2341 aaacgaaaaa ttagaacaga cgcgattaaa cacattgatg gtttaattac atcagacaat
     2401 gatttctttg ataatcaaac gccagaagat acaaagcagt tttttgaata tgctaaagcg
     2461 tttttagaac aagaatacgg taaagataat ttattatatg cgacagttca catggacgaa
     2521 aaaactccac atatgcatta tggtgttgtt cctatcactg atgacggtag attaagtgca
     2581 aaagaagttg tagggaataa aaaggcgtta acagcgtttc aagatagatt taacgaatat
     2641 gttaatcaac gtgggtatga tttggaccga ggacaatcaa gacaagttac aaatgctaaa
     2701 catgatcagg taagccgata taagcaaaaa acagaatacc ataagcaaga atatgagcgt
     2761 gagagccaaa aattaagcca tatacaacaa aaaagcagtg agttgatagt acaataccaa
     2821 aaatcattag aaacgcttaa aaagccttta aacgtccaat atgaacatga aactgaaaaa
     2881 gtaggtggat tgttcaacaa agaaatacaa gaaacaggga atgttgtaat aagccaagaa
     2941 gaatttaata actttcagaa acaaatacaa gcagcacaaa cgattacaga agattatgag
     3001 tatattaagt cagataaagc tctgaatgac ctaaaaaacg aaaatagtaa gcttagagaa
     3061 gaaaacaagg atatgtctga aactttagca agagcaaatg agtttataaa agacctcgaa
     3121 aaacacctta aaaaggcgct taatgttatt aaagttatta gagagatttt tgagagttag
     3181 agcaagctat tggtagaaat aaatatagga atttaatgaa tgatgtgagt agagataatg
     3241 ctcctatgat aaaggcaata caaatgtttg ataaacaaat acaccctgaa gaatatcaag
     3301 aagaaaagaa tacgcaaaaa cgtgataggg gtagaggtag ataaataaaa acgccactct
     3361 aaaaatagaa tggcgttcaa aatcttaaaa gaattgtttt aagtatatct aatttaatta
     3421 tacccacgtt tatgccgaga aaactttttg tcttaaagag tccttaactt agacttaaag
     3481 cgacagcggt cgatagcgtc agctattaac ccgaccgact gacagctttt aggattgtta
     3541 aggacgcaaa ctttaagacc aaaaggaaat tggaataaaa aaataaattg agttaatggt
     3601 ttaggtttta gggctaactt cacgaagtga tctgttagcc ctgtttttgt attttggctt
     3661 agaatttatc aaatttagtt ggaaaaaggg ggactactac gacccccctt ttaagtgccg
     3721 agtgccaaat ccgagattgt gaagtgttta gacctttaat gccaatggat tcaacgattt
     3781 ttcacaaagc tacatttagg ctacatttta gggactttat gagttattgt atagaaatct
     3841 ctagacatat atgtataaat ggaataaaat aaacttatta atagttagaa atggggagta
     3901 aaaatggttc gtacaaaagt aggtataact ttaactgaag atactttgaa aagacttgaa
     3961 gaaattgcag aacaaatggg tttaactaaa tctcaggcta tttctatgtt ggttaataaa
     4021 gaatatttga gtaagtatca agacaaagat aaagtattta aggcataaaa aaatgacttg
     4081 atctaattag gaactaatta gaccaagcca tttgatagtg ttatattaat aacaaaataa
     4141 aaaaaagaag tcgctcacgc cctgaccaaa gtttgtgaac gacaaaaccg taataaaata
     4201 taagaattat gttttattaa tttgtatat
//
