LOCUS       NZ_KM107848             7111 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain G1/2 plasmid pSYM12, complete sequence.
ACCESSION   NZ_KM107848
VERSION     NZ_KM107848.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867339
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2. G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: GS de novo assembler v. 2.5.3
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009963735.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:26:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 13
            CDSs (total)                      :: 13
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107848.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1/2"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM12"
                     /geo_loc_name="Germany: near Wetzlar"
                     /collection_date="1954"
                     /note="identical sequence found in strains G6/7 and G8"
     gene            complement(join(6958..7111,1..269))
                     /locus_tag="EL75_RS00005"
     CDS             complement(join(6958..7111,1..269))
                     /locus_tag="EL75_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            391..669
                     /locus_tag="EL75_RS00010"
                     /old_locus_tag="EL75_p0002"
     CDS             391..669
                     /locus_tag="EL75_RS00010"
                     /old_locus_tag="EL75_p0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(661..972)
                     /locus_tag="EL75_RS00015"
                     /old_locus_tag="EL75_p0003"
     CDS             complement(661..972)
                     /locus_tag="EL75_RS00015"
                     /old_locus_tag="EL75_p0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176703114.1"
                     /translation="MRYKTVDHEKRRRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQV
                     YTLTLADQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            1169..2668
                     /gene="csa"
                     /locus_tag="EL75_RS00020"
                     /old_locus_tag="EL75_p0004"
     CDS             1169..2668
                     /gene="csa"
                     /locus_tag="EL75_RS00020"
                     /old_locus_tag="EL75_p0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_104807859.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            complement(2696..3235)
                     /gene="csi"
                     /locus_tag="EL75_RS00025"
                     /old_locus_tag="EL75_p0005"
     CDS             complement(2696..3235)
                     /gene="csi"
                     /locus_tag="EL75_RS00025"
                     /old_locus_tag="EL75_p0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="WP_104807860.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMAIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            3265..3420
                     /gene="csl"
                     /locus_tag="EL75_RS00030"
                     /old_locus_tag="EL75_p0006"
     CDS             3265..3420
                     /gene="csl"
                     /locus_tag="EL75_RS00030"
                     /old_locus_tag="EL75_p0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            complement(3636..3749)
                     /locus_tag="EL75_RS00080"
     CDS             complement(3636..3749)
                     /locus_tag="EL75_RS00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_213849828.1"
                     /translation="MHEPPVQPDRCALSGNYRLESTPVRHDKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            4076..>4261
                     /locus_tag="EL75_RS00070"
                     /pseudo=""
     CDS             4076..>4261
                     /locus_tag="EL75_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4416..4607)
                     /locus_tag="EL75_RS00040"
     CDS             complement(4416..4607)
                     /locus_tag="EL75_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4675..4997
                     /locus_tag="EL75_RS00045"
                     /old_locus_tag="EL75_p0008"
                     /pseudo=""
     CDS             4675..4997
                     /locus_tag="EL75_RS00045"
                     /old_locus_tag="EL75_p0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5206..>5640
                     /locus_tag="EL75_RS00075"
                     /pseudo=""
     CDS             5206..>5640
                     /locus_tag="EL75_RS00075"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5693..6235
                     /locus_tag="EL75_RS00055"
                     /old_locus_tag="EL75_p0010"
     CDS             5693..6235
                     /locus_tag="EL75_RS00055"
                     /old_locus_tag="EL75_p0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_231201172.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            6242..6475
                     /locus_tag="EL75_RS00060"
                     /old_locus_tag="EL75_p0011"
     CDS             6242..6475
                     /locus_tag="EL75_RS00060"
                     /old_locus_tag="EL75_p0011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4244..4331
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3678..4220)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.978"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4568..4896
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.455"
                     /coverage="100.0"
                     /evalue="1.47e-62"
     Typing          4988..6582
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattcttttg cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc
       61 cccatatccc ggaactgctg aaagaacggg agcatcatac gtatggtgtc tgctttctgg
      121 gtatagaagt taggatcaaa tccatcatct gaagcataaa cataatgtcg ggtatgtcgt
      181 tccaggctgg tctttggggt gcatcctgta agagctattg ttgctgttat cagaggtatc
      241 agaagcagcc tggtagcggc agatctcata aacatctcct tgcgttttta atcttgatac
      301 tcagggagtt atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga
      361 tttgatggtc aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt
      421 agtgttcttt ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc
      481 gagaagacaa aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac
      541 agactgaaga tacaacttca gatcgatgag ttgagagcag agctggctgg caccagagat
      601 gcccaaaaaa gagaggattt gcgtaaaaaa ctggagtctc tggagcggca aaagaaatcc
      661 ttacgttgat gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata
      721 tttacgggcg aggcgaatgc gtcttgcctg gtataccgcc gagtaagcct gggtgggatt
      781 cacaccatta ctgatttcac tgaccacatg acgctgatca gccagtgtca gcgtgtaaac
      841 ctgtttctgg atgaggtcac gggctttgtc atgctcatat ccggcagcaa caagggtgtc
      901 gagaagtcgt ttgtctgttt tgttcagttt atggcgtctc cgtttttcat gatccacagt
      961 tttatatctc atcctgattt gctccggtgt tctgtttcga cgttctcaat gcctgcctcc
     1021 ccatagcgtg atccttatag caaaaatgaa aatccatgct cttgacatgg acaatagtga
     1081 gtagtaggtt ttgctgtaca taaaaccagt ggttatatgt acagtgttct gtttttaatt
     1141 tattgtttta attatcaaag aggaatttat ggctaaagaa ctaagtggat atggcccaac
     1201 tgctggtgag tctatggggg gaacaggggc gaatctgaat cagcaaggtg gaaataataa
     1261 tagtaatagt ggggttcact ggggtggtgg ttcaggctct ggtaatggag gtcgtgaaca
     1321 tggaagtcag acggggtggg gatggagtaa aaccaataat ccggatgtgc cgccttatgt
     1381 tgatgataat gggcaggtgc gcattaccat cacaaatggt ctggtaaaaa cgcctgtata
     1441 tggagttcct ggggctggcg gaaatagtga tgttcagggg ggctatatcc ctgaaaatcc
     1501 taatgatgaa gttgctcgta agtgggataa aaacaactta ccgcgagaga ttgatgtgag
     1561 tatcgatggt tttaaatatc gtgtaacgct taatgataat ggtagagcta tcggtattct
     1621 tcgaacaggt gtccgtcctt acgtagggag tgaaaaagct aaggcgggta ttatggaaaa
     1681 aatcaatcat aaaactcctg aggagatata tgaggcgtta ggttttaata aagatgaatc
     1741 tcagcggcag gaaaaagcaa aacaacaggc tgaagatgcg tgggatcgtt tgcctcctaa
     1801 tgtgagaaaa tttgatgttg atgttgaaca gttccactat ttagtggtgc ttgatgatta
     1861 tggtaatgtt ttatctgtaa ctagaacagc tgttcgtcct tatgtaggga gtgaaaaagc
     1921 taaggctggt attatggata aagtcgacca taagaccccg gaagagatat atgaggcatt
     1981 aggatttaat aatgaagagc ctcaacgaca ggatcaagct aaaaaagctg cttatgatgt
     2041 gttttattct ttcagtatga accgtgaccg gattcagagt gatgtactta ataaagccgc
     2101 tgaagttatc tctgatatag gtaataaggt cggtgattat cttggagatg cttataagtc
     2161 gttagcaaga gaaattgctg atgatgtcaa aaatttccag gggaaaacta tccgtagcta
     2221 cgatgatgca atggcgtcat taaataaagt tttgtctaat ccggggttta aatttaacag
     2281 ggccgacagt gatgcactgg cgaacgtctg gcgaagtatt gatgctcagg atatggcaaa
     2341 taaactgggt aatatcagca aggctttcaa atttgccgat gtggtgatga aggttgagaa
     2401 ggtccgggag aaaagcatcg agggctatga aaccgggaac tggggacctc tgatgctgga
     2461 ggtggagtcc tgggtgctca gtggcatcgc ttctgctgtg gccctgggtg ttttttcagc
     2521 cacattaggg gcatatgcct tatctcttgg ggctcctgct attgctgtcg gtatcgtagg
     2581 tattctactc gcagcagttg taggtgcatt aattgatgac aagtttgcag atgcactaaa
     2641 taaagaaatc attaaacccg ctcattaata aataccgggt aaaaggaagg cagggttact
     2701 gcctttcttt taataattta aacgtggtaa ctggtgtgaa tagcgtgaaa tagcttatga
     2761 aaaaaatgat taagtacagg ccaatataaa atagcaatag tgttgcatca ttgttggtca
     2821 ttagcctggc tggccttcct gctgtcgtta gctcaaggtt tcggaaacag agtaaataca
     2881 aaaaaacagc acagacaaat ggacttaata agcaacttct aagtacagca tttcggtttg
     2941 ttttttttat tagctttctc gttttaaatg tcaggataaa taatatcaaa gcaagggaag
     3001 gcgctgtttt acaataaata tccataacct ttgtcattaa tgggttgtag gcggaggtta
     3061 ttgatgggat gttttcagta ctggtagcta ttgtgtgcaa gaacagattg tctggatttg
     3121 tatagtaaac ggctagtata cagagtaaag gtataactcc gattgccata aataaataca
     3181 ataagttatt ggcttttcta ttgtctatat ctattgaatg ttcatcaatc atcattgacc
     3241 tataggatta taataaaggt atatatgaaa aaaattatta tttgtgccat ttcactagga
     3301 ataatgctgc ttgcagcatg ccaagtcaac aatgtcagag atactggagg tggggctgtt
     3361 tcaccatctt ccactgttac cggagtgagt atgggaagtg gtggtagtgg tagcctgtaa
     3421 aggatcttct tgagatcctt tacagatcgt cgtagtctct tgccctgtaa acgaaaaaac
     3481 cgcctgggga ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtagc
     3541 tggcttaagt ggagcacaga taccaaatgc tgtcctttca gtgtagccgc tataaggctt
     3601 caacttcaag agttctgcgt atctaaatcc acttttcagt taccaatggc tgctgccagt
     3661 ggcgctttgt cgtgtcttac cggggtggac tcgagacgat agttaccgga taaggcgcag
     3721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3781 gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3841 gcggacaggt atccggtaag cggcgggtcg gaacaggaag cgcacgaggg agcttccagg
     3901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagct acgcggcttt
     4021 ttttgccctt tgcctttttg ctcacatgtt cttttctgcc ttatcccctg attctgtgga
     4081 taaccgtatc gccgtttttg agccagcaga ctccgctcgc cgcagtcgaa cgaccgagcg
     4141 cagcgagtca gtgagcgagg aagcggaaaa gcgcctggac gagcattttc tccttacgca
     4201 tctgtgcggc atttcacacc cggcatggtg cacttttcat acaatcctca ccgatgctac
     4261 acgattaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     4321 cacccgctaa aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac
     4381 aggccagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg
     4441 aagtgtgcga ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg
     4501 tcagcctggt cgtcgacgtc cagttcattc agtttttcca gcagggtcag cgtctggctt
     4561 ctgatgaagc gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc
     4621 catgtgtggg gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc
     4681 acgatacggg tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa
     4741 cttgcgtcat ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt
     4801 ccgacactgg caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag
     4861 atagccagga agataaacag cggacagtgg tcgggcatga ccgggttcat gtggtggctg
     4921 cactgatggc catcggacgt gaactgagtg agcttaggga tgaggtccgt aaacagggag
     4981 aacgggatga tagttaagtt tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat
     5041 tacctgctgg gccgggagcg taaccgggaa ggggcgagag ttctgcgggg tgctcccgaa
     5101 gaggtacggg aactcatcga tgccacgccg tttgcgaaaa aatacacctc gggggttctg
     5161 tcttttgcag agcagaccct gccaccggga gagcgtgaaa gggtgatgga gagctttgag
     5221 cgggtactga tgcccggtct tgaaaaaaat cagtacagca tcctgtgggt ggagcaccag
     5281 gacaaggggc gtcttgagct gaattttgtc atcccgaata tggagctggc gagcggaaaa
     5341 cggctccagc cctattacga ccgggcggac cgtccgcgaa tcaatgcctg gcagacactg
     5401 gtgaatcacc attacgggct gcacgacccg aacgcaccgg agaacaggcg gacacttgtc
     5461 accccgaata atctgccgaa agcgaaacag gaggccgcag aagcgattac gaggggactg
     5521 gaggctcttt accatgccgg agcgctaaaa acgcgtcagg atgtcactga ggcgcttaca
     5581 gcggcaggtt ttgaggttgt gaggacaacc aggagcagta tcagtatcgc cgacccggag
     5641 ggcgggcgta atctcaggct gaaaggggcg ctgtatgagc aatcttttac aaatggggac
     5701 agacttcgag aagaaactga aagagcgtgc cgtctctaca gagaacatgc tgaacagcga
     5761 gttcaggaag ctcgaagcgt ctgtagacag ggaactgaac ttaaacgggc agaaaatcag
     5821 agacgctatc agcgcacaca cagcagcggt gaaagaacaa ctggagacgc tgaacagtgc
     5881 cgtgaacgcg cagttcagcg ccacagagaa cgaactggcc cggcggcagg aagagctgct
     5941 ctggaagctg atcaaagggc ggatactgta cccgtcgctg acggcgcttt gcgtcacggg
     6001 cgggattttt ctggcgtcat gggggctgat tcagtggcag gaaagccgga ttgcggcgaa
     6061 catcctggct atcagggacc aggaagagac gctggcgaaa ctccgggaaa aaacatgggg
     6121 tgtgacgtat cacgagggca ggaacgggaa atttctggtg ctgccgtccg gggtgaaggg
     6181 ggaaaacaac tggacggtgg agaagaagaa cgcagtaaga ctggtcaggg agtaagtgag
     6241 tatgacagag ctggaaatgc acttactgaa cgccttagag caattgcagc aggactatac
     6301 gcagcggctg aaagactggg agagcgcctt cacggatttg cagcatatgt tcgagctgac
     6361 acgacaggag aacgggcaac tgagggaaca gtgcgacgac ttgagtcagc aggtgcagag
     6421 attagccggg caggtggacc gcttgagtcg gttatttatc acgaacagca gataagggat
     6481 gaacaggaaa ggcaggcacg cctggaacgg gagcgtgcgc ggtcctggca tgaagagcag
     6541 gaacgggaag cacaggtatg gcgtggcccg tcaatgggga tgtgacggtg agttgttccg
     6601 ggaggttgcc agatggtacc ggaacgcgtt aaaaacgcat ccgggcgcga ttctggcgtg
     6661 tcgtttttgt tgctgtgttt tgcctctttg ccagatcgcg ctcattcctc agcctcatcc
     6721 cgcgatgtct gcgtatgtca tcaactgaaa agatggaaaa aaaacagtgt catgaggttt
     6781 acgcaggcaa aaccagcgtt attcacatgg ctgaatagct atgcatacgg ttaaaattta
     6841 tcaggtgcga tcgcggcagc ttttgggtgg tttgttgcca tttttacctg tcagcagccg
     6901 tgatcgcgct gaacgcattt cagcggtgcg tacaattaag ggattatggt aaatccctta
     6961 ctgtctgccc tcgtatccat cgagataaac cgcagaaatg gtgtctgcca ttagtttcat
     7021 ttttttcagc gaaggcatat cgctgtttgt gtattttctg ccagcaaagg ttgtagtgct
     7081 ctggagtgag tgaaaaaatt tttctgagtg g
//
