LOCUS       NZ_KM107847             3215 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain G5 plasmid pSYM11, complete sequence.
ACCESSION   NZ_KM107847
VERSION     NZ_KM107847.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: GE de novo assembler v. 2.5.3
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009963725.1-RS_2024_06_24
            Annotation Date              :: 06/24/2024 03:30:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107847.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G5"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM11"
                     /geo_loc_name="Germany: near Wetzlar"
                     /collection_date="1954"
     gene            complement(97..309)
                     /locus_tag="EL78_RS00005"
     CDS             complement(97..309)
                     /locus_tag="EL78_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039509481.1"
                     /translation="MGDFERTYGAGADFDRIIDENNSEYFRDERQKAKNSKKEFSSFQD
                     ALVWAKANPGKTIVRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(419..685)
                     /locus_tag="EL78_RS00010"
     CDS             complement(419..685)
                     /locus_tag="EL78_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_176703116.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAAGDLAVGK
                     PLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(669..893)
                     /locus_tag="EL78_RS00015"
     CDS             complement(669..893)
                     /locus_tag="EL78_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1056..1184
                     /locus_tag="EL78_RS00030"
     CDS             1056..1184
                     /locus_tag="EL78_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1439..1627
                     /locus_tag="EL78_RS00020"
                     /old_locus_tag="EL78_p7501"
     CDS             1439..1627
                     /locus_tag="EL78_RS00020"
                     /old_locus_tag="EL78_p7501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1675..1902)
                     /locus_tag="EL78_RS00025"
     CDS             complement(1675..1902)
                     /locus_tag="EL78_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            2757..2891
                     /locus_tag="EL78_RS00035"
     CDS             2757..2891
                     /locus_tag="EL78_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035952.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRFRGGES"
                     /name="replication initiation protein"
     Typing          2376..2866
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.935"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 catagactcc aacatacgac actgtgaaac atatgagtac gtatatagcc agtgttgtcc
       61 aaaaaatgac tgccgtttta cagtgaatcc actctttcat ttttcaataa atcccacacc
      121 gtctggagat cttactatcg ttttaccagg attagctttc gcccaaacca aagcatcttg
      181 aaaagaagag aattcttttt tcgaattttt agctttttgg cgctcatctc gaaaatattc
      241 gctgttattc tcatcaatta tccgatcaaa atcagcacca gctccgtagg tacgctcaaa
      301 atcccccatt tcctaccccc ataaatttaa taaaaaaaca gatttaaaat atccaacaga
      361 cactcttatt taccataaca tccgttgtca gcatcaaaaa aagcccgcat agcgggcttc
      421 agtcgtaaac ttcgcggcga tggccaatgg tggcggcaag gataaccagc tcgtcatccc
      481 ggatctcaca cagaatgcga taatctccca cccgatagcg ccagaactcg cccagctcac
      541 ctttgagcgg cttcccgacg gcgaggtcgc cggcagcaac tgcaatgcgt aaatccataa
      601 aatccacaat ccgtcgtgcg ttctgtttgt ccatcttgcg taacgatttg agcgcccggt
      661 cggaatagtt aatcgtccag accaagtgac ttcctcacgt cttcagacga atgcacggct
      721 tcatcaccac ggcgaacgcg tgcggcagtt tctgctgaaa gataataatc ttccaggtct
      781 tccagatgcg ccagtattgc ttcccgcgca tagaacgtct ttgttctgcc ggtttgcttc
      841 gccagcgagt ccagacggga ctcaatctca tcagataacc ggatagcaag catataaacc
      901 tcctgaacac catttgatat acatgtatag caatagtatc gcaatcaacc taaaagaaca
      961 aaaaataatc ttgctggccc ctcttgataa tccgtaacgc tgtcaatata tccagcttga
     1021 gggctttcct cttaactatt gaaaggataa cgattatggg tcttaagccg ggtccgaaac
     1081 gaattgccga ttcaacaggg gaacctgata aacgccagcg tgacaataaa aaaacgccgg
     1141 ggaatactga caaattgaaa cccagcaaat cctcaaaaaa ataagtcagt agttaaaagc
     1201 cctccggggc tttttttgta cccgcagatc agtctgattg aacataacat ctgttgcgtg
     1261 caccgcctga gaggctctca gggcgtttct acgacctcac ctgcaaaaga acacatctta
     1321 ccccctgaaa accgctcaga aacgcgctga tgatttctgc gtacctgcat ggctatgcac
     1381 tcaagtgaat gaagtaagat ttcagtctgc gatcaccata aaaccgggaa aaagccgtat
     1441 gacaaaacag gaatcagccg cactgaacat ggcaaaattt atccgggcgc agtcgctgct
     1501 gctgctcgaa aaactcgacg tgctcgatct tgatgacgag gcagccgact gtgagcgaat
     1561 gcacgaacag gcagaagcgc tttatcagaa gctgagcgca cgcttcggta tacaggacgg
     1621 cgaataaaga gacgaaaaga acgctcactt agacgaaaag cgtatccagg gcaactatgt
     1681 atatccaccg tgtacatagt catgcctgac gctttcgagc aggtcgagca tgcaggaacg
     1741 acgagcttga ttggtatcgg acacaatatg gcgatacgcc tcaaacataa cagcacccgc
     1801 atcaccgccc gaacggcgaa gcatagcgga aatcatccgc acaaagagca gacgttcatc
     1861 ggggcaaagg ttcataagtt ctcctggtga aaaaccaaac atgaaagccc ccatctatta
     1921 acgtcagctg tgaacgcttc attagaagaa aataggtaaa cccgggaaaa agatccaaac
     1981 gttaacatca caggtaaaca gaaacgtcgg gtcgatcggg aaattctttc ccggacggcg
     2041 cggggttggg caagccgcag gcgcgtcagt gcttttagcg ggtgtcgggg cgcagccctg
     2101 aaccagtcac gtagcgctag cggagtgtat actggctaaa cttcagagat gccaaggaag
     2161 ggacaggata tgttttgtag caccgccacc acggtggcga tactccaaaa cgcctggtca
     2221 gggcacagcc ccgacacccc agagccgact gtaggtcact ggacgaacgc ggcggaatga
     2281 tgtcggatga cgtcattttg cggcgtttgc cctatcctgc atcgtattaa aaaatgcagc
     2341 tatggcgcgt acggtgccaa agataagccc ggagatattc cgcttcctcg ctcactgact
     2401 cgctccgctc ggtcgttcgg ctgcggcgag cggtaccggc ttactggcgg ggcgggaaat
     2461 ttcctggcat cttccaggaa gaagcttaac agggaagcaa gaaggccacg gcgaagccgt
     2521 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
     2581 ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct ccctcgtgcg
     2641 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc
     2701 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     2761 acgaaccccc cgttcagtcc gactgctgcg ccttatccgg taactatcgt cttgagtcca
     2821 acccggaaag acacgacaaa gcgccactgg cagcagccac tggtaacagg tttagaggag
     2881 gagagtcttg aaatttcggg tgcgataagg ctacactgaa aggactattt tgtgactgtg
     2941 ctcctccaag ccagttaccg cggttcaaaa gagcgtcaac tcttgcgaac cttcgaaaaa
     3001 gccaccttgc cgggtggttt tttcgtttac agagctaaga gattacgatt ctccctaaat
     3061 gatctcaaga agatccgttt aaaaaagcgg gttttatggt tggatgaagt tcgtcttacc
     3121 cagtcacata ttagatttat ttgaattact actaggtttc gtaagccatg caagaattcc
     3181 tgtaactaac aaaataggcc ctgcaatgta cgtaa
//
