LOCUS       NZ_KM107846             1549 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli strain G1/2 plasmid pSYM10, complete sequence.
ACCESSION   NZ_KM107846
VERSION     NZ_KM107846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867339
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Velvet v. 1.2.10
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:28:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107846.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1/2"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM10"
                     /geo_loc_name="Germany: near Wetzlar"
                     /collection_date="1954"
                     /note="identical sequence found in strains G6/7 and G8"
     gene            complement(join(1516..1549,1..137))
                     /locus_tag="EL75_RS00005"
     CDS             complement(join(1516..1549,1..137))
                     /locus_tag="EL75_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001387432.1"
                     /translation="MGVWGSPQTVSGSAQGAAGTVAARFFLENGEQKHAKDFEAAKEQC
                     RIDNHGQNVNT"
                     /name="hypothetical protein"
     gene            1013..1489
                     /locus_tag="EL75_RS00010"
                     /old_locus_tag="EL75_p7001"
     CDS             1013..1489
                     /locus_tag="EL75_RS00010"
                     /old_locus_tag="EL75_p7001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          532..630
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(826..934)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.273"
                     /coverage="100.0"
                     /evalue="1.97e-45"
     Typing          complement(172..432)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 ctgcattgct cttttgccgc ctcaaaatcc tttgcgtgtt tttgctcccc gttctccaga
       61 aaaaaccgag ccgccacggt tccggcagcg ccttgagcgc taccggaaac cgtctgggga
      121 gaaccccaga cccccatcgc tgcaacaccg acggtctacg accacctacc aaccgcatgg
      181 ctgcctgttc cgtgcaaatg ggctttgcag ggggctggcg cggttcctgg cgacctgaaa
      241 gcccctggtt aggggtatcg gcgcgcttga cctcctgaga gcctctgtaa gcgtttttcg
      301 cgccttccat gccctggcgg gcatctaaaa cccgtttcgt gcgtctgaga gcttttgagc
      361 gcgtttgagg ggctatctgg cgactcatcc ccaaaagaac aacaccggga tcaccgacaa
      421 catgcgagag gtgatcccgc tcgccgcaag tcgatgaccg agcgtagcga gcgaatcgac
      481 gaggaagcgg aagagaaccg gaagccacat tgagcactta cgcactgatg cggggtgtcg
      541 gggtgaagcc ctgaccaagt ggtaatcgta tcggcgtgca tgcgcggtta tacgattaca
      601 catcctgtcc cgatttctga ggcgttttaa cggtgaacgg acggcaaaaa cacgatggag
      661 ctgttgaacg tgaaaaccac gaaatctggc aaaaatcacg gctggcgagg cttgcatagg
      721 agtaagccag tatacactcc gctatcgcta cgtgactggg tcagggcttc gccccgacac
      781 ccccaaaggg cgttgcgttg cacgcaacac ccttgcctag aatagatctt ttacgccgat
      841 ttgtaagtgg ttgttttttt agtgtgtttt ttatttacct gtcgcatcct ggatgacaca
      901 acgttgtcgc atccaggatg cgacaacacg caacaaggtg ttgcacagat gtgtcatgat
      961 ggcttagatt tgaaccaacg ttagtgcatg ggattttcag agggaaaaaa tcatgtttat
     1021 tgattcagaa aaacgactga aacaactttc agatgaggca aagaaaaaca ccgaggatct
     1081 cgaagaagca aagaaaaatt caaggtttac acaggtatcc ccaaaaggtt gggaacgtgt
     1141 tcgagagctg ctgaaggata gccaaggcat atcagcactg aagctgtact catttttagc
     1201 ggagcatatc gatcctacgt gtggcgctgt cgttgcggat cagcaattcc tagctgaaaa
     1261 acttggagtt agcagaagca caattattcg gtggctcaat tacttagaat caaaaaatgc
     1321 attagttaga atccccgttg ctggtaaggt ttgtgcgtat gccctcgatc cacatgaagt
     1381 ctggaaggga tacaacacta cgaaaaacca tgcagcgttt gtcactaaaa cactggtcaa
     1441 caaagacggt gatattcagc gccgaatcat ggccatgttt tcaaattgag ctagcggcag
     1501 gcggacaatc aggggctacg tgttaacgtt ctgaccatga ttgtctatc
//
