LOCUS       NZ_KM107845            12686 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli strain G5 plasmid pSYM9, complete sequence.
ACCESSION   NZ_KM107845
VERSION     NZ_KM107845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12686)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12686)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Velvet v. 1.2.10
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:24:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 15
            CDSs (total)                 :: 15
            Genes (coding)               :: 15
            CDSs (with protein)          :: 15
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G5"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM9"
                     /geo_loc_name="Germany: near Wetzlar"
                     /collection_date="1954"
     gene            complement(join(12604..12686,1..409))
                     /locus_tag="EL78_RS00005"
     CDS             complement(join(12604..12686,1..409))
                     /locus_tag="EL78_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(474..965)
                     /locus_tag="EL78_RS00010"
     CDS             complement(474..965)
                     /locus_tag="EL78_RS00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150916.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGK
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQVAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            1389..3263
                     /locus_tag="EL78_RS00015"
                     /old_locus_tag="EL78_p6504"
     CDS             1389..3263
                     /locus_tag="EL78_RS00015"
                     /old_locus_tag="EL78_p6504"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="WP_117150915.1"
                     /translation="MTRAKGVFPVIPRRCKAPRDCLFNFEDVKVSNHEENSGAVADNVT
                     PIDAAKGKPAADGSDLPFIRFSGGWVYLKTCKTDKKGETYYSLQAVCKSHLRVIGTGWV
                     DGEAFRVLELGKDKRRVMMPTRLIGSRDGWAFLQGHNVQVTPVPELRRWLHEYLMSETA
                     YDLHGSPCGVLPEWDVADKPGWRGGAYLLPDGDMIGDVGNVIADFKLRSNHADVGVCGT
                     VDSWVENVGRLLYGNDSPTLALGVAMASPMLGVLGVSGFGVHLYGQSSGGKTSSCYPAM
                     SIFGSPSNRQASWNSTPLALTLLARCCNDALMMLDEMKLLRAMVMEEAIYGTFSQRSRM
                     QGNKDGTLRPEYSWRCCVLSTGELAADVFIRMMTGRDADAGALVRLVQLPYRPLTNLHG
                     FPDGSAFADHLRAASDEHYGAVGRAWVDWLSSNQTLAREKFAEVKARWNGRGTHGQHQR
                     VADYFAVIETALTLSSKTLKLDVKECQGVLDRFYADWLVDFSKGGNGSHEEALLVERLE
                     AVLSQHQRFLSKTANDQDYKQPGELWGYFHDRGDAREYLLFPAVFKERISGTIDAKVAG
                     RLLHTRGLLRRHGEHLTVSERAGGYPNKRRFYALSLPVVDDLDAEEVL"
                     /name="DUF927 domain-containing protein"
     gene            3263..3448
                     /locus_tag="EL78_RS00020"
                     /old_locus_tag="EL78_p6505"
     CDS             3263..3448
                     /locus_tag="EL78_RS00020"
                     /old_locus_tag="EL78_p6505"
                     /inference="COORDINATES: protein motif:HMM:NF013937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_117150914.1"
                     /translation="MAKPTPAESAMNVTRFIRAQTLVMLEKVEALDFDDLTALAEKLHD
                     DAEKLERLVTDRVKNG"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4251..4718)
                     /locus_tag="EL78_RS00025"
     CDS             complement(4251..4718)
                     /locus_tag="EL78_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_192962653.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMPEGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5326..5886)
                     /locus_tag="EL78_RS00030"
     CDS             complement(5326..5886)
                     /locus_tag="EL78_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_192962655.1"
                     /translation="MACCVERDVSVRGEKKVNKTYGYVRVSTDEQTSNSQRDAIIARFP
                     TASILADDGISGTVPPRERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELE
                     KRKITLVSIREGVDLSSAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRP
                     TKIGAKEAGEIVMRRAMECRSPP"
                     /name="recombinase family protein"
     gene            6098..6757
                     /locus_tag="EL78_RS00035"
                     /old_locus_tag="EL78_p6514"
     CDS             6098..6757
                     /locus_tag="EL78_RS00035"
                     /old_locus_tag="EL78_p6514"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150913.1"
                     /translation="MTEIKTECPDAAWLRTTLAEMASDHFPVYDLPSLRVDPNSSTQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            6989..7330
                     /locus_tag="EL78_RS00040"
     CDS             6989..7330
                     /locus_tag="EL78_RS00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192962657.1"
                     /translation="MTLASLPLLSVVETVVETGGAAQTFAPIGAPVASPTEAVHRARAQ
                     SIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAFAEVRRQVHAGGMTLSP
                     AARGIDWNA"
                     /name="hypothetical protein"
     gene            7323..7685
                     /locus_tag="EL78_RS00045"
                     /old_locus_tag="EL78_p6517"
     CDS             7323..7685
                     /locus_tag="EL78_RS00045"
                     /old_locus_tag="EL78_p6517"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117150911.1"
                     /translation="MRELPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            complement(7945..8523)
                     /locus_tag="EL78_RS00080"
                     /old_locus_tag="EL78_p6518"
     CDS             complement(7945..8523)
                     /locus_tag="EL78_RS00080"
                     /old_locus_tag="EL78_p6518"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252368271.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKATKCEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCIATTWTATPPNQKPRRRPKTPKPA"
                     /name="hypothetical protein"
     gene            complement(8520..9851)
                     /locus_tag="EL78_RS00055"
                     /old_locus_tag="EL78_p6519"
     CDS             complement(8520..9851)
                     /locus_tag="EL78_RS00055"
                     /old_locus_tag="EL78_p6519"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192962661.1"
                     /translation="MLALSGDHFDAGGAVNDDNDTNESTQPNPAALERVSATEKFPEQL
                     PGVLFVTCPGGCSLRVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAP
                     LTTPADTRKLCPRCSRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRAPPFV
                     SPFLIGIGDGWQVWYGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHR
                     EPLRWNGGDGHAGEVELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNA
                     VWTASVCSDCKAAPLVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPE
                     FTQNETRSLAKIPVILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLS
                     VLAAVGRWDYINDIIRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            10165..10374
                     /locus_tag="EL78_RS00060"
                     /old_locus_tag="EL78_p6520"
     CDS             10165..10374
                     /locus_tag="EL78_RS00060"
                     /old_locus_tag="EL78_p6520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142910545.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRASDGVR"
                     /name="hypothetical protein"
     gene            10361..11209
                     /locus_tag="EL78_RS00065"
                     /old_locus_tag="EL78_p6521"
     CDS             10361..11209
                     /locus_tag="EL78_RS00065"
                     /old_locus_tag="EL78_p6521"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192962663.1"
                     /translation="MAFDNSHLADPGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSARHNRGSAHDRSQYRHFLKLKGVTNPEAMAQVFLQFSGEVYWRLDSKR
                     TPPTPEQVAALADRKRRSIEAVARREDADRRKAEKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDGSRTRPTRLCSSVRGSLRRSPCT
                     T"
                     /name="hypothetical protein"
     gene            11221..11541
                     /locus_tag="EL78_RS00070"
                     /old_locus_tag="EL78_p6522"
     CDS             11221..11541
                     /locus_tag="EL78_RS00070"
                     /old_locus_tag="EL78_p6522"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192962665.1"
                     /translation="MAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDAGEAYRLAHKAA
                     YGDDREPVPTIPGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRYAVRHARLE
                     RG"
                     /name="hypothetical protein"
     gene            11767..12222
                     /locus_tag="EL78_RS00075"
                     /old_locus_tag="EL78_p6523"
     CDS             11767..12222
                     /locus_tag="EL78_RS00075"
                     /old_locus_tag="EL78_p6523"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101998989.1"
                     /translation="MKSKAELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNALATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDEAA"
                     /name="hypothetical protein"
ORIGIN
        1 ctatcgccat tgcgaaaaag ccagccccag caacgttaat gcgacgaaca gcattgagca
       61 caatagtctg ctccgagtaa tctctgttaa cgtacacatt gctctgcacc gagaatgcag
      121 ttgccaaaat ctccgcacac tcttgccaac ttagcttatc cttcgagtta cgagcagata
      181 gcacatgcct aaacattgcc caagtgatcg gtattgctgg tgatgcaggt atgtcccagc
      241 cgtttctctg acaaacaact ttcatttgat tgaactgctt cttagtcatc agataggtaa
      301 aacaaggcgt ctcccaaact tcctgaggga gacctttatc atccatttct tcagcttgct
      361 gtgtaaaagc atagaaagcg tcacggatag catcatcacc gatagccact tcttttcctt
      421 gtaaaatttt tatgttcgaa ttttatcata tttagctatg tcacttagga ctgctaattc
      481 agtagcccac tttcccgcaa cttatggcga tagtcccgga cacggctggt gttccactcc
      541 tgtcccgtcg ctgtccggtg ccctgctgcg ttcagcgccg acgcgatggc gggcagagtc
      601 gcgttctctg cagtcatgct gacaatgagc tggtcggcct catcactgac ggcctggaag
      661 cggggatcgg ctccccgggg tactatctgt cttgcaggtt gttgtgcgct ttgtggtgct
      721 gtttgttgtg cgacttgtcg tgcaggttta ccgccgacgg acagcgccct gacttcagcc
      781 ctcagcgctt cgatggcagc taaaagtttg gtgtccatcc cttccggctt cttgccaagt
      841 ccggcgttca gcgcctctat gacagcatcc gtgcgggttg ctccggttcg tgctgcgttc
      901 tgctcgatac gttcgatcag gctaacgggt aggcgaacgg tcagggtttt ggttttctct
      961 ctcatggctg ctgctctccg tgtcgtgcgt tttgtcgtgc tgtttgtctt gcggagtgta
     1021 atgcgttttg tcgtgcgcgt cgaatttgac gtagtgcaat tgtcgtgcag ggaggggtag
     1081 cggctcggcc cgtggataac ttctgtggat aacaaaattt cgaacgttcc ggcccgtgga
     1141 taaatcgcgg aggctgcgcg gctctaagat cggtgcggga tcgcgtttcg cgtggttgga
     1201 tcgttgtgta ctggtgcagg ggggcatgta acggtatgat ggcgcggctg aaaaagaaaa
     1261 cccgtccggg cagacgggtc gttttcttaa agctgttttg agtttggagc tttcgcggcg
     1321 gtcaccgtgg aagacgccat aataactcat gttgggtgtg actccagcat gaattatacg
     1381 cgatcataat gacgcgtgca aagggtgttt tccccgttat cccccgccgt tgtaaggctc
     1441 ctcgagattg tctgttcaat tttgaggatg taaaagtgtc aaatcatgaa gaaaattccg
     1501 gcgctgttgc cgacaacgta acgccgatcg atgctgccaa gggcaaacct gccgccgatg
     1561 gctctgacct gccgttcatc cggttttccg gcggctgggt gtacctcaag acctgcaaga
     1621 ctgacaagaa gggcgaaacc tattactcgc tgcaggctgt ctgtaagtct cacctgcgtg
     1681 tcatcggcac cggctgggtc gatggcgagg cgttccgagt tctggagctg ggaaaggaca
     1741 agcgccgggt aatgatgccg acgcgcctga ttggctcgcg agatggctgg gcgttccttc
     1801 aggggcataa cgtgcaggtc actccagttc ccgagctacg tcgctggctc catgagtact
     1861 tgatgtccga gacggcctac gatctgcatg gctctccgtg cggcgttcta cccgagtggg
     1921 acgtggctga caagcctggt tggcgcggtg gggcgtacct cctgccagac ggcgacatga
     1981 taggcgatgt tgggaacgtg atcgccgact tcaaactgcg aagcaatcac gccgacgttg
     2041 gtgtgtgcgg taccgtcgac tcctgggtgg aaaacgtagg gaggctgctg tacggcaacg
     2101 actcgccgac cctggcactg ggggtggcga tggcctcacc tatgcttgga gtgctcggcg
     2161 tgtccgggtt tggcgtccac ctgtacgggc agtcctccgg tggtaagacc tcctcctgtt
     2221 acccggctat gtcgattttc ggatctcctt caaaccgtca ggcttcgtgg aacagcacgc
     2281 cgctggcgct tactctgctg gctcgctgct gcaacgacgc actgatgatg ctagacgaaa
     2341 tgaagctcct ccgggctatg gtcatggaag aggcgatcta cggcaccttc tcgcaacgat
     2401 cgcgcatgca ggggaacaag gacgggacgc tgcggcctga gtattcctgg cgctgctgcg
     2461 tgctctcaac gggtgagttg gctgctgacg tattcattcg catgatgacc gggcgtgatg
     2521 ctgacgcagg ggcacttgtt cgcctggtgc aacttcccta tcgcccgctg acaaacctcc
     2581 acggcttccc tgacggctcc gcgttcgccg accacctaag ggcggcatct gacgagcact
     2641 acggtgctgt tgggcgggcg tgggtggatt ggctgtcgtc aaaccagact ctggcccggg
     2701 aaaaattcgc agaggttaag gctcgttgga acgggcgggg tactcatggc cagcatcagc
     2761 gcgtggcgga ctacttcgcg gtgatcgaga cggcgctgac gctgtcctct aaaacgctca
     2821 agctcgacgt gaaagagtgc cagggcgtgc tcgatcgctt ctacgccgac tggctcgtcg
     2881 atttcagtaa gggtggcaat ggctcccatg aagaggcgtt gctggttgaa cggctggagg
     2941 cggttctgtc gcaacatcaa cggttcctgt ccaagacggc gaacgatcag gactacaagc
     3001 agcccggcga gctgtggggc tacttccatg accggggcga cgcgcgcgag tatctgctat
     3061 tccctgcggt gttcaaagag cggatctccg gcaccatcga cgccaaggtg gcggggcgct
     3121 tgcttcacac tcgtgggctt ctgcgtcgtc atggtgaaca ccttacggtg tcagagcgtg
     3181 ctggcggata cccgaacaag cgccgcttct acgcgctgtc tctccccgtg gttgacgatc
     3241 tggacgctga ggaggtgctc tgatggctaa acctaccccc gctgaaagtg ccatgaacgt
     3301 gacgcgtttt atccgtgcgc aaactctcgt catgcttgaa aaagtggagg cgctcgactt
     3361 cgacgacctt accgctcttg ctgaaaagct ccacgacgat gccgaaaaac tggagcgcct
     3421 tgtcaccgat cgggtgaaaa acggctgatc ggtagcactg gaaccagtcc actggttcca
     3481 aacgaaaaac ctctacagcc cgcgccctgc gggctttttc atgcaattca cccgtaaaat
     3541 cgaaaaacta ctggttccgc actggttcca gctacaggcc gcgccgttgc tgggctccag
     3601 cgatttggaa ccagtggaac cagtggaacc ggtgggttgt ttgtctctat ggggatctcc
     3661 cccaagttgc ggcaatcagg aaagtctatg cgcacatacg tgtattattt tttactggtt
     3721 ccactggttc cactggttcc agtgtcctct aaggcgcgtg gttgctgggt ttgttttgga
     3781 accagtgaaa aataatgact ggttccagac tggttccact ggttccactc ggtgggtgta
     3841 atgggtgtaa tggttaaaac ctgcgcagtc gccgtcacgc agctgcaccc cgttgggctg
     3901 cacttttgac gctatggggg atctgtcgat gggttcgtcg tgccggggtc gtgggtggtg
     3961 gtggttcgga gggggatcgg tggtttgggt ggcgctgagg gcacgagctg tcgctcgcgc
     4021 tgggtggtcg tctgttggtt ctgcgtgagg gcttgccctc gcgccctctg cgttatgggt
     4081 ggaatgcgtt gctgcagctg cgcgtttggt tcgtccgggc atcaaaaccg gggcttctcg
     4141 cgcacgcgag cggtaacaat tgcgtaacgt ggggatccgg gtattccgtg ggctcccggg
     4201 ttttggcggt cacaaaaacg gtcacatggc ggtcatttcg gtcatggcgg tcattgttgt
     4261 ttgcggttca tgccctcggg catgatgtcg gcctcggtgc ccggcttgat ctcgtagatg
     4321 aggcgttcgc gttcctgcgc tttgagcagg gcggtcatga ggccgctgat cgctttgacc
     4381 atggtggcgt ccttctccag catggcctgt aacgatgcgc tgcggagggc ttgcaagtcc
     4441 tttgcgtggc gctgtagcag gtcaaattgc tgctgtacaa agtggttttt aaggcgtcgg
     4501 atcgcggcct gtggtgtggc ctcgacggcg gcggcggcgc gtttccgcat ctcctcgatg
     4561 ctgaggacgt tgctgggtgc tgcggccttc tcgctcgccc atcggcgaac ggtgcggggt
     4621 gggatcccca tggtgcgtgc gacggtggca ctggttgcgc cgtcctgctc cagtagcctg
     4681 agcgcctggg ctttctgttc ggtggtgtat ttcttcatgg ttcctgctcc ggttggtttc
     4741 tctcccgtat tgaaacgtca cgcgctgaaa atggcggggg tttttggggg gtacacggcg
     4801 tttgtgcgtg cgtggtcgtg cggttgggtg tttttgtggc tgaggcgctt agaatggctc
     4861 tggtgcgttc tggtgaatgg ggcgcttccg cgcccctgta attggttcgt aatggcgtaa
     4921 ggcttttgtt attgcttgct gcttacacct gtaaggcttt cgttatggtc atgcttccca
     4981 tgcgtaaagg ctgatgatgc tgacggtgcg gtatcgccct gcctggtcgc ggagggtagt
     5041 ctggtactgg cctcggcggt tggcggcgca atgacgcggc ttgccaaact gggggatctc
     5101 gccgtgtttg atgttcaggc ccgggtagcg gtgctctgca tgtcattaaa cctctaacgg
     5161 gtggttggtg atgcccgggt tggctctcac acttccccgg gcctgggttg cggcggtgac
     5221 tttagcgagt accaccgccg cgatctctct cttactccgg cgcgaccgcg tcgaccttct
     5281 caagatggcg gtaaatcgcg ctccggctta ggtttagctg tttggctatg gtggcgatcg
     5341 gcactccatg gcgcggcgca tgacaatctc cccggcttcc ttcgctccga tcttggtcgg
     5401 gcgtccgaac ttctggcctc ggctctttgc tgcctcgata cctgctgcct ggcgttcgag
     5461 gatgagcttg cgttccatct gggcgaagct ggccattacg ccgagtaccg cctgtccgac
     5521 ggcgctgctc aggtcgacgc cctcgcggat gctgaccagg gtgatcttcc gcttctccag
     5581 ctcctcgacc agctcgaggg tgtccctggc gttcctgccg atgcgatcga tcgcgtaaat
     5641 gaccagggtg tcgccctcct gcagcaactg gttcaagagg atgccgaggg cagggcgctc
     5701 gcgtggtggt accgtcccgc tgatcccgtc gtcggcaagg atggaggcgg ttgggaaccg
     5761 ggcgatgatg gcgtctcgct ggctgttgct ggtttgttcg tcggttgata cgcgaacgta
     5821 accgtatgtt ttgttcactt ttttctctcc tctgaccgat acgtctcttt cgacacaaca
     5881 tgccacaact gtatcgaaag tctatcgcta aagtgtcttt ttcgtcgttc tgttgggtgt
     5941 cctgaaatgt aggggattag cgacggcctc caggcggctc cccgcgctgg gcgagagccc
     6001 tgcgccctct gcgttgatgt ctgcgctgcg tcgtgacggc atgctgtctg agcggcgttt
     6061 cttgccgctt tgctgataac ttgctgagaa ccaaaccatg accgaaatca aaaccgaatg
     6121 cccggacgcg gcctggctgc gtaccacgct tgctgaaatg gcgagtgacc attttcccgt
     6181 ttatgacctc ccgtccctgc gggtagaccc gaactcctcc actcagcttt cggcgctcgc
     6241 tgatcgtcag gctgcgcgtg aaatgcgtca ggccgcttcc gacgtagagg cgcgacggct
     6301 ggacgccgct cgagtggtgg aagggctcaa gaccgaggcc gaacggctgc ggggcttgat
     6361 cgccgacggc aaggctgcgc tgcgtgctgg cgagccggtg agccccgacg cgggtgttgc
     6421 gtcgttcctg ctgccggaca tcgaggccga gctggttgtt gctgaggctg ctgaggccga
     6481 cgtggcccgg gagcgtgaca ccctgctgca ggatgccgat cgccgtgacg cggcggctgc
     6541 gctggcgctg ttcaactggg cgcactccgt tcgcgtgcag cgtatcgagc tgcttctcaa
     6601 gctggcgatg gacgaggcga cgacgctcgc tgaaaccgac ggcgggcgcg gcctgtaccg
     6661 taccgtgatc gcgcctgacc gccgtcttaa ccaaattttc gccacgcagg gcgctattga
     6721 aatcctgcgc cgtaaccgtg gcatggaggg tgtttaatgt ctgctttcaa actcctgtgt
     6781 gaccggcttc gcaatgaggc gacgctggca aaatctgacg gggccggcgc tttcaaaaag
     6841 caatctctgg atcccgacct gaagggcggc aagaagggca aaaagcccgc cggggagctg
     6901 gaggaggaca aggcgatcgc gtctctccac cgtaagcgca tgaacgacgt taaaggttgc
     6961 aagacgctga ccaaatccac cggcgctgat gactttggcc tcccttccgc tgttgtcggt
     7021 ggtcgagaca gtggtggaga caggcggcgc agcgcagacg ttcgcaccga tcggggctcc
     7081 ggtggcgtct ccgaccgaag cggttcaccg tgcgcgtgcg cagtccatcc aggacgcggc
     7141 gttcctgaac gagatgaaag cggcgggtgt ggcaccggct gacctggtgt tacttgacgg
     7201 cgtgttgacg tcctctcgtg ggatgccaga cgcggcgcgt gctccggcgt ttgccgaggt
     7261 gcgccgtcag gttcatgcgg gcggcatgac gctgtcaccg gctgcgcgtg gtattgactg
     7321 gaatgcgtga gctgccgcgt cacctgcagg gcgaggtgtt gatgccgctg tccagcagga
     7381 tcacgcgctc gcaacggctg gagctgatct ggctgttgcc cgttctgcgg ctccacccgg
     7441 gcgatcgatc ggtcactgag cggcgggcgc ttggcaggtt ccggggatcg ctcgctgcgg
     7501 gtggccagac ggatgctttc gtccgttcgg atgcgtctct gtacgcgctg ggactgcggt
     7561 tccagcgcaa gcgcacccgg gagctggttg cttcggatcc cgatgttgct gcggctggcg
     7621 aggcgctgga gcagcgtgca aacggcgtga acctgtccga gacgttcggg ggtggcgatg
     7681 agtgaatttg ctgtctcgcg cctggtcatt cctgaggtgg tgatccccga cggggtcatt
     7741 gctgcccgac gggcaagggg acaggctcaa cgtgtcgcag ctgctcgccg tgctgcagag
     7801 cgtcgtgctg cgcctacgtg gcgctgggtg agacgggaac agccccggtc gacagggcgg
     7861 tgaaacggtg agacggggcg cgttatgcgc cctttttttc gcccttcgcc tggcgtgcct
     7921 tgcgccggaa ttcccggatc tctgtcaggc gggctttggc gttttcggcc tcctccgggg
     7981 tttctggttc ggtggggttg ccgtccaggt cgtagcgatg cactggtcga tgaggctctg
     8041 ctggtacgag ctggtacccg tgacaatgcc caggatgcga cggacggctt tgcggtctgc
     8101 ctctggcggc atgtccagct ctgcgcctcg ggtcaggatc tgctcgatga tgccctttgc
     8161 catggggcga cggtcaactc cccgggtcag taagtcaggc cagtgctctt tcaggaatgc
     8221 tttggtcacc cggttggcca gcgccttttt ggacttgttg gcgatcgcga tgtcctgctg
     8281 gcggcggtcg ttctcctcct gtctgcggat cccctcggcg acctgctcgg gcgtcttttt
     8341 cggctttgcc gctgtgggct tctgtgcggg cttctgcggg gctttcgctt ttggctgtac
     8401 cttcgccttg ctggtagtcc gttcggctgg ctgtggctca catttcgtgg ccttttccgg
     8461 cgggacgtag accgtgcggt tgcgtcgctt gatggtcagg atcgggcggg tgggttcggt
     8521 cataaatctc tctcaggtct gttggtctgg atgtggtcgg caagggcgat cagggtggcg
     8581 gcgtcgccgc ggtcccgatc gatgcgccgg atgatgtcgt tgatgtagtc ccagcgcccg
     8641 acggcggcaa ggacggaaag ggtgaggcgg ttgaaccgac tctctggtac ccggtcgagg
     8701 gtcaggggga tgcgcttgcg ggggcgtcct ggaccgcgcc ctgtccctgg tcttttgagc
     8761 agctgcggct gctccctctg cggcaaaata acggggatct tcgccaggct gcgcgtctcg
     8821 ttctgcgtga actcgggcac cgcgatgtgc tcgtctgtcc atgtcctgcc tccgggcctg
     8881 gctgatgcgc tggagtcgca ggacgggcag gagacgacga tctcccgtcg cggcttgccc
     8941 ttgaccggtg ccttgctgat caccaggggc gcggctttgc aatcggagca gaccgacgct
     9001 gtccagaccg cgttgtctgg cagggtggcg ttccggtact ggtgcgcggc gtcatgcggt
     9061 gtctggaagc tgaccgtctg aaacgtgggg gcgacgctga ggggtgccgc ccatggctgg
     9121 cacctgggac aagcaagctc cacctctccg gcgtgcccgt cgccaccgtt ccagcgcagg
     9181 ggctcgcggt gttcgtggca gtagtacacg aaaccgtcgc ggcgtgtttg cccgacaggg
     9241 cctggtcggc gatcgtggaa ccgtgcgccc gggttggctc gcatggcgcg gacgagcgcc
     9301 tcgccctggt ggatgccgta ccagacctgc cagccatcgc cgatgccgat caggaacggc
     9361 gagacgaacg gtggggcgcg gttgactctc ccgcgtgcgt tggctccgat ggtggcttcg
     9421 cgttcgcggt tccagcagga gacgcacagg ctcgaaccgg gcttattggg ggcgatcagg
     9481 cggttgtctt tccggctgca acgggggcag agcttacggg tgtctgccgg ggtggtcagt
     9541 ggtgcgtcag ggggctgttc ggtaccgacc caaagcgcga ggccagtggg gcaccggtgg
     9601 cactcgaggt gggggttttc aatcgtgcga tcgaggtggc tctgctcata aatctgtcgg
     9661 catgcctcga cccggaggga gcacccgccg gggcaggtga cgaacaaaac gccggggagc
     9721 tgctcgggga atttttcagt cgcgcttacg cgttcaagtg ctgccgggtt cggctgcgtc
     9781 gattcgttcg tgtcgttatc gtcgttcact gctccccctg cgtcgaaatg gtcgccagac
     9841 agcgccagca ctggctgtgc gtctttttta gtatctcaca aaaaacgcca ctttggcgtt
     9901 aattgtgtta cgcttgtctt actgattcct gacgagcagt tgggaaacat gaaaccggat
     9961 cgcccggtgc cgctttccct cctccccaac gaggcggggt tgtaacctag gatgcgcgct
    10021 tcgtccttgg taggtgcgag taagcgagaa gggctgctgt cctgccgcta tgcggtaagt
    10081 cctcaagggc ggctttatcg ataatcgtca ctcccgcccc ccgatgcccg ttaagctgag
    10141 gggggcacct atctggtact cccaatgaaa aaacgaatct ataccgctga cctcgctgcg
    10201 ctgctgaacg tgtcgctgcg cacgctctcg cggctaagtt cgcgggggat cgtgccgact
    10261 ccggcagggc gctccctccg tggcgggcat tactggctgg ctggcaatcc cgagatcaaa
    10321 cgcttcgttg ccgaccagcg tcgtcccggg agggcttctg atggcgttcg ataactctca
    10381 cctggctgac ccgggcctgt tctggcgtgc tgtgtctgct gcgggggctc cggttgaccg
    10441 tgaggactgg ctgcgcctct gctccggcat cgcgtgctgg gcgtctctct gcggcgagga
    10501 tgggctggcg gctatcgagg cctggtctgc ccgccataat cgcggctctg cgcatgaccg
    10561 gtcgcagtac cggcacttcc tcaagctgaa aggcgtaacc aatcctgagg caatggctca
    10621 ggtgttcctg cagttctccg gcgaggtgta ctggcggctc gactcgaagc gcacgccgcc
    10681 gacgcctgaa caggttgctg ctctcgctga ccggaaacgg cgatcgatcg aggcggtggc
    10741 cagacgcgaa gatgccgacc ggcgcaaggc cgagaaggct gcggctgatg cggtcaggat
    10801 gctggcgaaa ggcgacggta atccggccta taccccgtac tggcgcaata agaccacaat
    10861 cgccaggcgt ggtggcgtca gcctcccgcc cctccccgct gaccttcgtg tgggtggctt
    10921 gttccgttcg ttcgaaccga gggcgtactg gtcgcgctgt ctgcttgttc ctgcctatcg
    10981 cctgaccgac gatcgccagc tggttggcga cgcgctggag gccatctgcg gcgtctgctc
    11041 tgctggcggc aaaaccggcg acaagttctc ccctgcgggt acccaaaagg cgggacggtt
    11101 ctatccgatg cccggcttta tggacgtgct cgacggctcc cgcacgcgcc ctactcgcct
    11161 ctgttcgtct gtgaggggtt cgctacggcg ttcgccctgc accacctgag ccgtggcaag
    11221 gtggcggtct gcgctgcgtt ctcgatcgcc gggttcggca gctgcgtggc gtcgctgcgt
    11281 cggttctggc ctgatgttga tgtgatgctg gtaccggacg caggcgaggc gtaccggctg
    11341 gcgcataaag cggcctatgg cgacgatcgc gagccggtcc cgacaattcc aggggcgtcg
    11401 gtggtgtcgt tcgacggcct gaaccgcttc gagccccgcg ataacctcga ctggttcgat
    11461 gtgctggttg aggagggcga ggatcgggcg cgtgagctgc tccggtatgc ggtgcgtcat
    11521 gcccggcttg agcggggctg attggcgtgt ccgaatggtg cgcctttcgg gacatctttt
    11581 ttgccgtcgt ggcgtctggt ttagcgaacg actttcgaga cactaccgat gcttttttga
    11641 tgagacggac taaagcgacg caccggcgct gccgtcgtga cgctatcctg cgatcggttt
    11701 ctcacagcag cataaagccc ggcctcgtct tgatgtcggg ctttttttta gcccaaagga
    11761 gggcacatga aatcaaaggc tgaactacgc gaggccgcga cggctcgttc gctggcggtg
    11821 atcgccactg aaatgtcgga gtggtcgctc gacgggttct cccacctcaa gctgccgaac
    11881 ttctccgctg gggaacgcca gcagacgctg tccgggtcgg tggtggttga tcgcccgccg
    11941 ttcgactacg agtgggctgg taccgagaaa tttaacgcgc tggccacccg tgcgctgcag
    12001 gtgaaacttc cggcatcgcg tgagcgtaac tacgcctggt tgtgcggcgt tgaacgggag
    12061 acgctggcga cggcgctcct cgtggagctg ttctccgtca ccggctgcgt ggcgttcgcc
    12121 gggctgggta aggttgccga tctggcgttc ctgacgctgg acgagagcga ggcggggcag
    12181 atcagggcgg cgatgctgca gtggctcgat gaggcggcgt aagccgtctc tcctctgcgt
    12241 caaatcagac cgcgctagta gcagcaaaaa agagaagaaa tccccgcgag accgctcctc
    12301 gtgccgtctg gtcataaaca aacctgttga atctacgtca acaatagcct actattattg
    12361 tactgcaaga gcattcgcag ggggtattaa ccagtaatta accctttgcg ctgggcagtc
    12421 aggaggctgg tctcctgttg ggaagttatg ttgcggcttc ctagtaataa cagtgaattt
    12481 gatagacagc agaaaaccgc cgatgctgct agatccgaaa ggaaccaatg aagggaaata
    12541 tttaggcgtt atggttcatt ctcattaaat tgaggagaat tgcttaaatg ggcgtccttc
    12601 ctatcaccct cgctgtatag ctttacattt ggcctccgtt gcatggtatg cagtcacggg
    12661 ggccaaatta ttttcagaga catcaa
//
