LOCUS       NZ_KM107844             2353 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli strain G5 plasmid pSYM8, complete sequence.
ACCESSION   NZ_KM107844
VERSION     NZ_KM107844.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/4, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Velvet v. 1.2.10
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:20:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107844.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G5"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM8"
                     /geo_loc_name="Germany: near Wetzlar"
                     /collection_date="1954"
     gene            190..918
                     /locus_tag="EL78_RS00005"
                     /old_locus_tag="EL78_p6001"
     CDS             190..918
                     /locus_tag="EL78_RS00005"
                     /old_locus_tag="EL78_p6001"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_012564910.1"
                     /translation="MGNSKRNIKKLNDNFREDILDYAIAHNLKCANALAILYATGCRPD
                     ELQTGVTVNYDSKKNEIEFRIIGSKLNRRMRRGIGVRKIKVKINNENARFFKNIVDKFI
                     ENPMSYDHKIKIESAKAFSGYITKISKKLWPRKTYHASAYSFRHAKATELKNSDYDKIE
                     IAQIMGHASVRSQQSYGRKSKKSKGGFDDIADVETNVKPRGGDRLLRFKIANKNKAAAK
                     IADTSTPSSPPPAPVRRFKM"
                     /name="site-specific integrase"
     gene            1425..2171
                     /locus_tag="EL78_RS00010"
                     /old_locus_tag="EL78_p6002"
     CDS             1425..2171
                     /locus_tag="EL78_RS00010"
                     /old_locus_tag="EL78_p6002"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_172687180.1"
                     /translation="MDIGNILNESLNVEYSKLDLFLEKYDLTPEQKVEVYEFHAKASKK
                     NKTLVISETKENKFKSISEGVEYVHLFPKNLKLLIKKYGLNTNELLVLTEIMESMLSHG
                     NLLINFSQKTLCELTEINKSTMSKTFKSLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFI
                     QFRDHFLNSISYKLDDEEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFE
                     KEISEEWKEKFKDEEENFEFGFESEM"
                     /name="helix-turn-helix domain-containing protein"
     Typing          980..1163
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.459"
                     /coverage="100.0"
                     /evalue="1.26e-89"
ORIGIN
        1 attttaggga tagagcaaag cgaaggccat aaaattgcca cccccaaccg ggggtcgttg
       61 ttcgatttga gcgatagcga aaaattgaac ataagggggg agggtttggg ttttacggta
      121 tttcaaattt gagcaaagcg aatttttgaa atttccggtt cttttaattt gcaatgagga
      181 aaaatcaata tgggtaattc aaaaagaaat ataaaaaaac taaatgataa ttttagagag
      241 gatattttag attatgcgat cgcgcacaat ctaaaatgtg ctaacgcact tgctatttta
      301 tacgcaacgg gttgccgtcc ggacgaactc caaaccggag ttactgtaaa ctatgacagc
      361 aaaaaaaatg aaattgaatt tagaataatt ggatcaaaac taaatagaag aatgagaaga
      421 ggcatagggg ttagaaaaat aaaagtaaaa atcaataatg aaaatgccag attttttaaa
      481 aacattgttg ataaatttat tgaaaaccca atgtcatatg atcacaaaat caaaattgaa
      541 agtgccaaag cattttccgg gtacataaca aaaatatcga aaaagctatg gcccaggaaa
      601 acctatcatg cttctgcata ttctttcaga catgcaaaag caacggaatt gaaaaattcc
      661 gattatgata aaatcgaaat agctcaaatt atgggccatg cctcagttag atctcagcag
      721 agttacggaa gaaagagcaa aaaaagcaaa ggtggatttg atgacatcgc agatgtcgaa
      781 accaatgtta aaccccgtgg cggagataga ttattgagat ttaagatcgc aaataaaaac
      841 aaagcagcgg caaaaattgc cgatacttcc acccccagca gtcctccacc ggctcccgtt
      901 cgtcgcttca aaatgtgaac cgtgagcagt tcaggaggtt ccctcctgga ctgtgaaggg
      961 ttggcccgtc cggcaggacg gttttacagc aaaatcctcc atagcgaagc agaagcccgg
     1021 aaccgggtaa ctggatggtt ttcccccgtg ggggattgat ctgttacttg aaaaccaatg
     1081 atcttaaaag ccatctcaaa agttgaaaat ttcaccccct tagtgttctt aaaattctta
     1141 gatgttctta ggagttaaaa aactactctc taaccattga tattactgac tttttaaaaa
     1201 aggcagttgt caaaaacttc aaccgtagtt gtcaaattcg tcaactctag ttgtcaaatt
     1261 cgtcaactga ggttgtcaaa tccgacaact ctagccagtt tccaagtaga aactacagtt
     1321 tcaaaactga aactttttct accttttgca actcaatcta ttgactagtc ctttataaat
     1381 gttaaaacat atatatagaa ataaataaaa aagaggaggt ttctatggat attggaaata
     1441 tattaaatga gagtttgaat gttgaatatt caaaattaga tttgtttttg gaaaagtatg
     1501 atttaacacc tgagcaaaaa gtagaagttt atgaatttca cgcgaaagcg tctaagaaaa
     1561 ataagacttt agttatttcg gaaacaaagg aaaataaatt taaatctatt tccgaaggtg
     1621 ttgaatatgt gcatttgttt ccaaaaaact taaaattatt aattaaaaaa tatggtttga
     1681 atacaaatga attattggtt ttaacagaaa taatggaatc gatgctttca cacggaaatt
     1741 tattaattaa tttttcacag aaaactcttt gtgaattaac agaaattaat aaatctacaa
     1801 tgtcaaaaac atttaaaagc cttaaacaaa agcagtgttt aattgagaaa aacgggcata
     1861 tttatttaaa ttctgtaata tttatgaaag gtttgccgca taaattgttt attcaattta
     1921 gagatcattt tttaaattca atttcttata aattagatga tgaagaagag tttgaaaaag
     1981 tcttcgacga taattttatt aaagcatatg aaaaaaatct caaagaaatt aaaaagaaaa
     2041 aacaacagat aaaagaaaag aaaatatcaa aagcattaga taattttgaa aaagaaattt
     2101 ctgaagaatg gaaagaaaag tttaaagacg aagaggaaaa tttcgaattt ggttttgaat
     2161 ctgaaatgta aaaccgccct cgccgggcag gcgaatccct tactgaaata gaaataaatt
     2221 ctattccact aagggatttt ttttatccat tgattctcca catttcaaat attgacagca
     2281 ttttccaccc ggttataaca gtactataag tagttgtttc aacccgtctt tttgggtgga
     2341 acaacaaggc acg
//
