LOCUS       NZ_KM107843             4452 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain G5 plasmid pSYM7, complete sequence.
ACCESSION   NZ_KM107843
VERSION     NZ_KM107843.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867357
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009963685.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:23:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107843.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G5"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSYM7"
                     /geo_loc_name="Germany: near Wetzlar"
                     /collection_date="1954"
     gene            403..726
                     /locus_tag="EL78_RS00005"
                     /old_locus_tag="EL78_p5501"
     CDS             403..726
                     /locus_tag="EL78_RS00005"
                     /old_locus_tag="EL78_p5501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001347450.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            935..>1369
                     /locus_tag="EL78_RS00040"
                     /pseudo=""
     CDS             935..>1369
                     /locus_tag="EL78_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1404..1889
                     /locus_tag="EL78_RS00015"
                     /old_locus_tag="EL78_p5503"
     CDS             1404..1889
                     /locus_tag="EL78_RS00015"
                     /old_locus_tag="EL78_p5503"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023139312.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1893..2108
                     /locus_tag="EL78_RS00020"
                     /old_locus_tag="EL78_p5504"
     CDS             1893..2108
                     /locus_tag="EL78_RS00020"
                     /old_locus_tag="EL78_p5504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_058671277.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2279..2755
                     /locus_tag="EL78_RS00025"
     CDS             2279..2755
                     /locus_tag="EL78_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250695668.1"
                     /translation="MSFTVASAENITYTKPSAGRDLYLTCKGYQVKGASIINVERPSYL
                     EGTGEQVEFAIISLGDASDGLKVFFDSWGFSSFGLIESHVMHEHWSLVHVGGRVSVKGE
                     KIDYSFVDNQPQEGGSYKPKTGVFLYGCINSDKRPSWVEKIYTRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(2948..3262)
                     /locus_tag="EL78_RS00030"
     CDS             complement(2948..3262)
                     /locus_tag="EL78_RS00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176703108.1"
                     /translation="MKILPAEPGLYFFASFESGAHVYKVVAWSENENGGLDPQCVLTAL
                     YDKSSSSQQWELHSTSASAYQHSNDGRPFMYFITYFTSNGGIPELYNAINTLADDGDTH
                     "
                     /name="hypothetical protein"
     gene            complement(3346..3777)
                     /locus_tag="EL78_RS00035"
                     /old_locus_tag="EL78_p5505"
     CDS             complement(3346..3777)
                     /locus_tag="EL78_RS00035"
                     /old_locus_tag="EL78_p5505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(4107..4220)
                     /locus_tag="EL78_RS00045"
     CDS             complement(4107..4220)
                     /locus_tag="EL78_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          117..299
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.737"
                     /coverage="86.0"
                     /evalue="2.11e-43"
     Typing          complement(4108..4452)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.859"
                     /coverage="86.0"
                     /evalue="1.37e-122"
     Typing          717..2200
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.919"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cggcaacggc cgcatccttt ttcagccctt cctgatttcc gcttagtctc tttatccagc
       61 cattcgtgag ctggcaacgc ccgccgcagt ctcacggcag gagcgcagcg accgagtgag
      121 cgaggaggcg tcatttcacc gggatctgca tttcgcactt acacaccggt gctgcatttc
      181 ccggtaccac atgaagcact ttcgtaaacc attactgcgt tgtaacattt taagccagta
      241 tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaacct gctaaaacca
      301 ctgacgcgcc tgcggcttgt ccaacaccgc gccgaccggg aaaagatttc ccgcccgtcc
      361 cggcgtaatc aggaggccgg attggcacac aaacgcagca aaatgctcac catgtgggta
      421 acggaagatg agcaccgccg tctgctggag cgctgcgacg gtaaacagct tgcagcctgg
      481 atgcgccaga cgtgcctgga cgagaaaccc gcccgcgccg gcaagcttcc ctcgctctcg
      541 ccggcgctgc ttcgtcagct tgccggcatg gggaacaacc tcaaccagat tgcccggcag
      601 gttaacgccg gtggtggcag cgggcacgac cgcgtgcagg tcgtcgccgc gctgatggcc
      661 atcgatgccg gactcgagcg gctgcggcat gccgtgctgg aaaagggggc agacgatgat
      721 cgttaagttt cacccgcgag ggcgcggcgg tggcgccggt ccggtggatt atctgctggg
      781 gaaagaccga cagcgcgacg gcgccaccgt tctgcagggg aagccggaag aagtcaggga
      841 gcttatcgac gcctcgccct acgcaaaaaa gtacacatcc ggcgtcctgt cctttgccga
      901 aaaggattta ccgcccgggc agcgcgaaaa gctgatggcg agctttgaac gtgttctgat
      961 gcccggactc gataaagacc agtacagcgt gctgtgggtt gagcaccagg ataaggggcg
     1021 gctggagctg aacttcctga tcccgaacac ggagctgctg accggcaagc gtctccagcc
     1081 gtattacgac cgcgccgacc gtccgcgcat tgatgcctgg cagaccatcg tgaacggcag
     1141 gctggggctg catgacccga acgcgcctga aaaccggcgg gcgctggtca cgccgtccgc
     1201 gctgccggaa acgaagcagg aagccgctca ggcaattaca cggggtttac tggccttagc
     1261 ctcgtccggg gagctgaaaa cccgccagga cgtcactgag gcgctggaaa gcgcaggttt
     1321 tgaggtggta cgcaccacaa aaagcagcat cagcattgcc gacccggacg gggggcgaaa
     1381 catccgactg aagggagcca tctatgaaca gtcttttaac gctggcaaag gacttagagc
     1441 agaaatcgaa agcgcagcag cagagtaccg gcgagatgct gaaagccgca ttcagcgagc
     1501 acgagaagtc tgtcagagcg gaactgagcg aaagcgagaa gagaatcagc gccgccatcc
     1561 tcgaccacga ccggaagctg tcctcagcca tgagccagcg cacgaaaggc atggtgcgta
     1621 tggtcagcca gacgtggctg accatcgtcc tggtgtctgc actgctgaca gcatcgggcg
     1681 caagcattct gtggtggcag gggcagcaga tactcgacaa ttacacgacc atccgggagc
     1741 agaagcgcac gcaggccatg ctgtccgaga ggaacagcgg cgtgcagctc tcgacctgcg
     1801 gagagcagag acgccgctgc gtgagggtga acccggaagc gggacggttc ggagaggatt
     1861 cgagctggat gatactggcg gggaaatagc acatgacgga actggaaaaa cagttgctga
     1921 gcgcattaga gcagctacag caggactact cgaaaaggct ggacgagtgg gagaacgcct
     1981 tcgcggaatg gcggacgatg tctggtctta tgcaacggga gaacgcggcg ctgagcgagc
     2041 gcgtcacggg cttgagcagg caggtgcaga gtttgagcga tcagctgcgc cggttgtcga
     2101 aaggctgaac tcgattgaag ctcacaggca gcaggagcgg gcggcgcagc atcagaaaac
     2161 gctggagctg gaacgatcgc agcggcagaa aggttttgag cttggaatgt agttaatcaa
     2221 tgaaaaggga tgcgttatga agtgtttctg ggcttgctca gtctcagtag ttttggttat
     2281 gtcatttaca gtcgcttctg cggagaacat tacatacaca aaaccgtcag ccgggaggga
     2341 cttatatctt acctgtaaag gttatcaggt gaagggggcg tctataatta acgtcgagag
     2401 accttcttac cttgaaggta caggtgagca ggttgagttt gcaatcattt ctctcggtga
     2461 tgcatctgat ggactcaagg ttttttttga ttcatggggt ttttcttctt ttggattgat
     2521 tgaaagtcat gtgatgcatg agcactggtc tttagttcat gttggggggc gtgtttcagt
     2581 caagggtgaa aagattgatt attcatttgt agataaccag ccacaggagg gtgggagcta
     2641 taaacccaaa acaggtgtgt tcctgtatgg ctgcattaac tctgataaaa ggccatcttg
     2701 ggtcgaaaaa atctacacaa gaacaggcgt gtttcgaggg ggcatagaag actaaaatac
     2761 tgcgtacagc atgaaaattc attgaacaga gtgttagttc attttcgtgc atggctatgc
     2821 atacacgcaa aacttatcgg cgcgattccg ggcggttttc ggtgagaaga cgggctcttt
     2881 tgggctcagg acggaaaaac gcctaagggg tttagcggtg cgcgcaagag atgttatggt
     2941 aaatcaatta atgggtatcc ccatcgtcgg ctagtgtgtt tattgcgtta taaagttcag
     3001 gtatcccgcc attgctggtg aagtaggtga taaaatacat aaatggtctg ccgtcattgc
     3061 tgtgctgata tgcagatgct gaagttgagt gaagttccca ttgctggctg gaactgctct
     3121 tgtcatataa ggccgttaac acacactgtg gatctaaacc accattctca ttttctgacc
     3181 aggccactac tttgtaaacg tgagcaccgg actcaaaaga tgcgaaaaaa tataatcccg
     3241 gttcagctgg taggatcttc attagctagc ttccttttgt ttattctgtc tgagagacaa
     3301 taactaccta attccccgtg gtgggtataa aaaatcacgg ggaggttaat aacctaaacg
     3361 ttttagttct gctgctaaac gcttaagact ctcatctaaa tactcgtgat tctcttttag
     3421 ctcgtttttc attaaatcaa tgtgacgaga taactcagga ttgcctcctg aacgactgac
     3481 cggaaagtcg tcgctggaag ataagcggcc atgtttactg taatccattg ctatgcagat
     3541 aagcgttgcc aagtcgataa cacgatgaag aggtaattct tctgattgcc gtgaccactt
     3601 ttctccggtg ttgcgccaca cttttgctga tagctcggta tacccggggg tgttccattg
     3661 agcaattcca acggaaagcc ccatagcatc agtgtcatca gcataaggtc catcaattcg
     3721 atcatagttt tctacagcga taatcggttt gtgtttcaga tgagtaggga tgttcatatc
     3781 attctctttt atgtgaattt aggttgttaa ttaataaaat aacaaaacaa cttattttac
     3841 taattagtaa gtgtaacatt ttgtgaattg atggatttga agttgttggc gtggatcttc
     3901 ttgagatcat ttatggctga gcgtaatctc ttgctctgaa aacgaaaaaa ccacctgggg
     3961 aggtggtttg atcgaaggtt aggtcagttg gggaactgct taaccaggta actgtcttgt
     4021 gcgagacgag tcaccaaaac tgtccttcca gtgtagccgt tctcaggctc ccacttcaag
     4081 aactccagat aaatctctcg cacatctcag ttaccagtgg ctgctgccag tggcgctttt
     4141 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg acaaggcgca gcagtcggac
     4201 tgaacggggg gttcgtgcat acagtccatc ctggagcgaa ctgcctaccc ggaactgagt
     4261 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaag
     4321 ggcaggaatg cgggggagca caaggaaccc tccaggggaa acgcctggta ttttaaagcg
     4381 gctcgggttt cgccaccact atttgagcgt ctgatttttg atgctcggca gggggcggag
     4441 cctatggaaa aa
//
