LOCUS       NZ_KM107842            10433 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain G3/10 plasmid pSYM6, complete sequence.
ACCESSION   NZ_KM107842
VERSION     NZ_KM107842.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867340
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Zschuettig,A., Auerbach,C., Eichhorn,C., Blom,J., Goesmann,A.,
            Jarek,M., Scharfe,M., Zimmermann,K., Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: GS de novo assembler v. 2.5.3
            Sequencing Technology             :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009963675.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:27:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107842.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G3/10"
                     /db_xref="taxon:562"
                     /plasmid="pSYM6"
     gene            join(10232..10433,1..473)
                     /locus_tag="EL76_RS00005"
     CDS             join(10232..10433,1..473)
                     /locus_tag="EL76_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_172687184.1"
                     /translation="MNPFKGRHFQRCKYGISYRELQEMLAERGVNVDHSTIYRWVQRYA
                     PEIEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDFYLSSRRNSKA
                     AYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIE
                     CDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVF
                     EM"
                     /name="IS6 family transposase"
     gene            598..1422
                     /locus_tag="EL76_RS00050"
                     /old_locus_tag="EL76_p8001"
     CDS             598..1422
                     /locus_tag="EL76_RS00050"
                     /old_locus_tag="EL76_p8001"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001141174.1"
                     /translation="MPSSLKHNVVTVSVEASDRSEDQHHCVKVRFEEWDSLIDELGDET
                     SAVKVTKKLCAGRVSFDCDCGRHQYWYRYIATAGNFALAPPKEYAFPKIRNPNLKGIAC
                     KHVIHAMTRLQSASWQLRIGQAMLQAAKRVGFGDDKRRTTKHFTEEDRKRFNKNRNSQT
                     NQGAMRQEWDKYQRRQKALGNQIARDSTKLRTLSDKLLKARKMTQKQRAKAEESQQKLK
                     AEQDKNKVLLQQLADRFKVERQAFIDAMVMTGVSRQDAEKRFLDYVKNKGRG"
                     /name="hypothetical protein"
     gene            complement(1459..1878)
                     /locus_tag="EL76_RS00015"
                     /old_locus_tag="EL76_p8002"
     CDS             complement(1459..1878)
                     /locus_tag="EL76_RS00015"
                     /old_locus_tag="EL76_p8002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000990392.1"
                     /translation="MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPD
                     HPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGVALLRVLMDQHKLTQSDFEEEIGKK
                     SLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1875..2186)
                     /locus_tag="EL76_RS00020"
                     /old_locus_tag="EL76_p8003"
     CDS             complement(1875..2186)
                     /locus_tag="EL76_RS00020"
                     /old_locus_tag="EL76_p8003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family toxin"
                     /protein_id="WP_000558568.1"
                     /translation="MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRF
                     ASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKIFIKHITTHAEYDKLTDFYRRTKE"
                     /name="type II toxin-antitoxin system HigB family toxin"
     gene            complement(2416..5424)
                     /locus_tag="EL76_RS00025"
                     /old_locus_tag="EL76_p8004"
     CDS             complement(2416..5424)
                     /locus_tag="EL76_RS00025"
                     /old_locus_tag="EL76_p8004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_000718520.1"
                     /translation="MAADFLTDKQTQNYGRYAAEPNEIQLARYFHLDERDLTFINLRRG
                     RHNRLGIALQLTTARFLGTFLPDLMQIPPGVQFYVARQLNIRYPEIISRYAQRENTRWE
                     HHGLIRQHYSYHDFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLLPAA
                     STLTRVIGEIRERATRRLWRKLAALPNRWQAAQLAGLLEIPEGQRLSVMEHLKRGPVTI
                     SGPAFTEALERYTRLRSLEFSCLNFTGLPAIQLRNLARYAGMASVKYISRMPEERRLAI
                     LTAFVKAQEISALDEAVDVLDMLILNITREAKKTGQKKRLRTLKDLDRAALLLARACAL
                     LLDEDTGDDLLRKTIFSSVPVARLAESVEKVNELARPQDTNFQDEMVEQYGRVRRFLPA
                     LLRDLHFRAAPDGEHTLAAIHYLAELNGSKKRILDDAPEHIISGPWKRLVYDAEGRIQR
                     AGYSLCLLERLQDALRRRDIWLENSDRWGDPRQKLLQGEEWQAQRVPVCRALGHPTNGS
                     KASVQLAARLDETWKTVASRFDRNTAVDICNEGKHPSLTISSLDKLDEPPALIRLSSRV
                     RQLLPPVDLTELLLEIDARTGFTREFSHVSESGARAQDLHISLCAVMLAEACNIGHEPL
                     IKHNIPALTRHRLSWVKQNYIRAETLVSANARLVDFQSSLALAGYWGGGEVASADGMRF
                     VTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQQT
                     GLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRADKAANYGALDELARG
                     CVDLSKIESHWDEMMRVAGSLKLGTIHASELIRSLLRSTRPSGLAQAIMEVGRVNKTLY
                     LLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRYREGQEDQLGALGLVTN
                     AVVLWNTLYMQEALSHLRSTGEGPEDEHIARLSPLMHGHINMLGHYTFTLPEDIMKGEL
                     RPLNLNLNNELPP"
                     /name="Tn3 family transposase"
     gene            5583..6164
                     /locus_tag="EL76_RS00030"
                     /old_locus_tag="EL76_p8005"
     CDS             5583..6164
                     /locus_tag="EL76_RS00030"
                     /old_locus_tag="EL76_p8005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001196201.1"
                     /translation="MALLGYARVSTSHQKLTSQVTELNTAGVRDDRVFTDMMSGATDER
                     EGLQRLLARAEKDDIILCTKMDRLGRNTADMIRIVDTCYKKGIAIRFLENGLSTEGTMG
                     KMVIQILAAVAEAERERILERTNDGRQAAMIKGVKFGRKPHRKAELANELINKDVEVKE
                     VMEKTGISRATYFRLKRKTVTNNINLSNNK"
                     /name="recombinase family protein"
     gene            6241..7632
                     /locus_tag="EL76_RS00035"
                     /old_locus_tag="EL76_p8006"
     CDS             6241..7632
                     /locus_tag="EL76_RS00035"
                     /old_locus_tag="EL76_p8006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="WP_172687185.1"
                     /translation="MSQYLKLGDDQSPVQLDPHSEVGAFKVVGHCAWAKPEDKITPDFL
                     RSRVEPWLTALFQSEHLNILIGAGLSSAIQESATGTKPQGMGWIEDLKVCKAEIDSYVA
                     KTADASGRGKGNIEDQIRSINELIKGLEILTVQNLPVPPSPPGAPSYRNLKSELVELNN
                     ELTRCLKLFSDSVSYGEKLIRDANNDLKTETFNYLVSFLISFSSRTATRDRLHIFTTNY
                     DRIIETGAEIAGLRLIDRFVGTIAPIFRSSRLEVDYHYNPPGIRGEPRYLEGVARFTKL
                     HGSLDWYATEGAIRRFGLPFGASSVEPFLQVETTGTANYQQLMIYPNSVKDRETSEYPY
                     VELFRDLASATCRPNSTLVTYGYSFGDEHINRVIIDMLTIPSTHIVIIAYGDPLGRIMR
                     FINDSGRKAQISLLLGDHFGDIKKLVDFYLPKAAIDRSSIRMAELLKSRGLYRATTSNN
                     SGDSE"
                     /name="SIR2 family protein"
     gene            7629..9692
                     /locus_tag="EL76_RS00040"
                     /old_locus_tag="EL76_p8007"
     CDS             7629..9692
                     /locus_tag="EL76_RS00040"
                     /old_locus_tag="EL76_p8007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084041.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_001084040.1"
                     /translation="MNSFPIEQGEQLRVGTVDFVSPNEIRALLDIDSPDAVALNAGTPR
                     NFPRVNSYVLVSCDNGYLVGQIEWLAVEHSPYPKQRDIQDFGLVNLPFPRKKISLNPVG
                     TLKRFSKDGTDYFKFQRGSESFPSIGSAILLPTDLQLRSIVESGDNRRIVIGQSPLANN
                     ANVAVDPDRLFGRHIAVLGNTGSGKSCSVAGLIQWSLEAAMESEVKPNARFIILDPNGE
                     YVRALGPTTKFKGRIFKVEAGENEHQLQVPSWFWNSSEWASFTQASPKAQLPLLKRSLR
                     AMRNEDFELETTSNIEIKRFLGNVLITLKHDKSSGKPFGAWGPAKGFNEIMGKWIESFE
                     GYRDKLGNNNLTNVITIMSKYRQDRNVQYATMQATVPEIDSIIESVTSAFREFGGFEKE
                     LLPKNEDIPLPFDGNNLVAYLEALAQENGSEQYVEYLVARIRTMLSDTRMRSVTNDADN
                     RIDLAKWLEKYLGTDCSRDDNSCISIIDLSLVPSEITHIVTAVISRIIFESLQRYRRLY
                     NKSLPTVLVAEEAHTFIKRYRDDSENQDVAAVCCQVFEKIAREGRKFGLGMVVSSQRPS
                     ELSPTVLSQCNTFLLHRISNDKDQEQVHKMVPDNLRGLLRELPSLPSQHAILMGWASEL
                     PVLVKMKNLTKDQQPHSDDPDFWDVWTRKYADGKLVERTVDWGAVVKEWQQK"
                     /name="ATP-binding protein"
     gene            10007..>10138
                     /locus_tag="EL76_RS00045"
                     /pseudo=""
     CDS             10007..>10138
                     /locus_tag="EL76_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /name="IS5/IS1182 family transposase"
ORIGIN
        1 atgaaaccta cgtgaaggtc aatggccgct gggcgtatct gtaccgggcc gtcgacagcc
       61 ggggccgcac tgtcgatttt tatctctcct cccgtcgtaa cagcaaagct gcataccggt
      121 ttctgggtaa aatcctcaac aacgtgaaga agtggcagat cccgcgattc atcaacacgg
      181 ataaagcgcc cgcctatggt cgcgcgcttg ctctgctcaa acgcgaaggc cggtgcccgt
      241 ctgacgttga acaccgacag attaagtacc ggaacaatgt gattgaatgc gatcatggca
      301 aactgaaacg gataatcggc gccacgctgg gatttaaatc catgaagacg gcttacgcca
      361 ccatcaaagg tattgaggtg atgcgtgcac tacgcaaagg ccaggcctca gcattttatt
      421 atggtgatcc cctgggcgaa atgcgcctgg taagcagagt ttttgaaatg taaggccttt
      481 gaataagaca aaaggctgcc tcatcgctaa ctttgcaaca gtgccgccgg aagtgaggcg
      541 atcgacgtta aacgtgctaa caacgccgta gacgacggtt ccggcattaa gcgcgccgtg
      601 ccttcatcat tgaaacataa cgtcgtcaca gtcagcgtgg aggcttcaga tcgctccgaa
      661 gaccagcatc actgcgtaaa agtgcgcttt gaggaatggg attcactgat tgatgagctg
      721 ggggatgaga ctagcgcggt aaaagtgacg aaaaagctgt gtgccggtcg cgtatccttc
      781 gattgtgatt gtgggcgtca tcaatactgg tatcgctaca tcgcgaccgc cggtaacttt
      841 gccctggcac cgccaaaaga atatgccttc cccaaaatcc gtaaccctaa tctcaaaggg
      901 atcgcctgta agcacgtgat ccacgcaatg acccgactcc agtcggcaag ctggcaattg
      961 cggatcggtc aggccatgct gcaggccgct aaacgtgtag ggttcggtga cgacaaacga
     1021 cgtactacta agcatttcac tgaggaagac cggaaaagat tcaacaagaa ccgtaactca
     1081 cagactaacc agggggcgat gcgtcaggag tgggataaat accagcgccg ccagaaagcg
     1141 ctgggtaatc agattgcgcg agacagtaca aagctacgca ccctcagcga caaactgtta
     1201 aaagcccgga aaatgacgca aaaacaacgg gcaaaggcag aagaaagtca gcaaaagctt
     1261 aaagccgagc aggataaaaa caaggtgctg ctgcagcagc tcgccgaccg tttcaaggtg
     1321 gaacgccagg cgtttattga tgcgatggta atgactggtg tatcacgtca ggatgccgag
     1381 aaacgcttcc tggactacgt gaaaaacaaa ggcaggggtt aatacaaaag cccccaacct
     1441 ttcggtcagg gggctgtttt acgcgtccat gaatgagctg ggcggaatgt tgaagcgccg
     1501 cgcaagtgct ttcatgtgat ctagtgttag tgagcgagtc ccgttaagga tgcggctcac
     1561 cagcgatttc ttaccaatct cttcctcaaa atcactctga gtcagtttgt gctggtccat
     1621 caggacacgc agcagggcca cgccacttgg taacgctgca atacggtcgt tgaactctgc
     1681 gaactcttcc gcctcatctt catactgggc aattttcgca accagcatgt ccaccagcgg
     1741 atgatctggc tcgtgttcaa ccaggtattc aaccagcgtc agcgcgtctt catagtcctt
     1801 gcgagaggca ttgcccccca ggagggggac aatgctaacc aggctgtttg ccgcctggat
     1861 agcatcggtg tacatcattc tttagttctc cggtaaaaat ctgtcagctt gtcgtattcg
     1921 gcgtgtgtgg ttatgtgctt tataaagatt ttcccacgtt caaaatcagc aaaaaacatc
     1981 acccttaaat tcccaccgcc aacatcgatc acccaccact tctcccgata tttcattctg
     2041 tccagcgagg caaatcgttt tttcatttcg tccggtgaag tgtagttctc tctcttcaaa
     2101 gtccggtata cgtcgtctag tgccgctgcc tgattgggga attgcctggt ggctgtatcg
     2161 aaaggtgctc tcgatacaac gtgcatcctt cgctctcctt gtgtttccat cttggaaaca
     2221 taatatcgca ctcccgcaga gttgacaagt tggaaactga aaataattag tgcctctgca
     2281 attcacaatc tccatacagt gctcacatac gcaaattaac cccgtcagtg aggcactgaa
     2341 tgggccgttt tgatgaatgg ttagacgaca caaggccggt aaggggtttg agggccaatg
     2401 atgtaaaaaa ccacgctaag gaggtaattc attgtttaaa ttgagattta acggcctcaa
     2461 ttcccccttc ataatatcct ccggtagtgt gaacgtataa tgtcccagca tgttgatatg
     2521 accatgcatc agaggcgaaa gccgcgcgat atgttcatct tctggccctt ccccagtgct
     2581 acgcaggtgc gacaaggctt cctgcatata aagcgtattc cacagcacca ctgcgtttgt
     2641 caccagaccc agcgcaccca actgatcttc ctgcccctcg cggtaacgtt ttctgatctc
     2701 accacgctga ccgtagcaaa tggctcttgc cacggcatgg cgaccttcgc cccggttaag
     2761 ctgtgttagg atcctgcggc gatagtcctc atcatcaata tagttgagaa gatacagtgt
     2821 cttgttaact cttccgacct ccattattgc ctgagcaagc cctgacggtc gggtacttct
     2881 caatagcgat cgaatgagtt ctgacgcatg aattgtgccc agcttcagcg aacctgcaac
     2941 ccgcatcatc tcatcccagt ggctttcgat tttagaaagg tcaacacaac cccgagccag
     3001 ttcgtccaac gccccgtaat tcgctgcttt gtcagcccgc cagaacaccg cctcaccagc
     3061 atccgccaga cgaggtgaaa attggtatcc cagtagccag aacaagccaa agataatgtc
     3121 actggtacct gctgtgtccg tcatgatttc aaccgggttt agtcccgttt gttgctcaag
     3181 tagtccctcc agcacaaaaa tggaatcccg cagcgttcca gggatcacta tgccatgaaa
     3241 acccgaatat tggtcagaga caaagttgta ccaggtaatg ccgcgccctg aaccaaagta
     3301 ttttctgttg gggccagaat tcaccgtttt cactggtgtg acaaaacgca taccatcagc
     3361 ggaggccacc tcaccgccac cccagtatcc tgccaacgct aacgaggact gaaaatcaac
     3421 cagccgcgca ttggcgctga ccagcgtttc tgctcggatg tagttctgtt tcacccagct
     3481 caggcgatgg cgggtcagtg cagggatatt gtgcttgatc agcggctcat gtccgatatt
     3541 gcaggcctca gccagcatca ccgcacacag gcttatatgc agatcctgcg cgcgggcacc
     3601 tgactcgctg acatggctga attcacgggt gaaacccgtt ctggcgtcaa tctcaagcaa
     3661 cagttctgtc aggtcaacgg gtgggagcaa ttgtctgact cggctgctca gtcgtatcag
     3721 cgccggtggc tcatccaatt tatccaggct gctgatggtc agcgaaggat gtttaccttc
     3781 attgcagatg tctacggcgg tattgcggtc aaaacgtgat gccaccgttt tccaggtttc
     3841 atccaaccgt gctgccaatt gcaccgatgc tttactgcca ttggtggggt gacccaatgc
     3901 ccggcacact ggaacccgct gcgcctgcca ttcctccccc tgaagaagtt tttggcgcgg
     3961 atcaccccaa cgatcgctgt tttccaacca gatgtcacga cggcgtagcg catcctgaag
     4021 tcgctccagc agacacaacg agtaacccgc tcgctgtatc cgaccttcag catcgtacac
     4081 caggcgtttc caagggcctg aaatgatatg ctcaggtgca tcgtccagga tccgtttttt
     4141 cgagccgttt agctctgcca ggtaatggat ggccgcgagg gtatgttcac cgtctggggc
     4201 cgcccggaag tgcagatcgc gcagtagtgc gggcaaaaaa cgccttaccc gtccgtactg
     4261 ctccaccatt tcatcctgaa agtttgtatc ctgggggcgg gccagttcat tcactttttc
     4321 cactgactct gccagtctgg cgacaggtac actgctgaat atggtctttc gcagcaaatc
     4381 atcaccagta tcttcatcaa gtaaaagtgc acatgcccgc gccagcaaca acgccgcacg
     4441 gtcaagatcc ttgagcgtcc taagtcgctt tttctgcccg gttttcttcg cctccctggt
     4501 aatattgagg atcagcatat ccagcacgtc aacggcctcg tccagcgccg agatttcttg
     4561 cgctttcacg aacgcggtaa ggatagccag ccgccgttct tctggcattc tgctgatata
     4621 tttcaccgat gccattccgg catagcgtgc caggttgcgg agctgtatgg caggcagccc
     4681 agtgaaattc aggcaggaaa attccaggct gcgcaggcga gtgtaacgtt caagtgcttc
     4741 agtgaatgcg gggccgctga tagtgacagg gcctcttttt aggtgctcca tcactgagag
     4801 tcgctgtcct tcggggattt caagtaaccc agccagttgt gcggcctgcc aacggtttgg
     4861 cagcgcggcc aattttcgcc ataagcggcg ggtcgcacgc tcacggattt caccaatgac
     4921 cctcgtaagg gtggatgcgg ctggcaacaa cactttattt tgaagcaacc acgcggtggc
     4981 aaaatcgaac atcagtccgg ggcgttcatt actgagccat gcacgagtat acagcaatcg
     5041 cttcagtctg aacgaccacg ggaaatcacc gaaatcatga tagctgtagt gctgcctgat
     5101 aagcccgtgg tgctcccaac gtgtattttc cctttgagca tagcgggaga tgatctctgg
     5161 atagcggatg ttaagttgcc ttgcgacata aaactggaca ccgggcggta tttgcatgag
     5221 atcgggcaag aaggttccca gaaagcgggc ggttgtgagt tgaagggcaa tccccaaccg
     5281 gttatgcctg cccctacgta ggttgatgaa agtaaggtct cgctcatcaa gatgaaaata
     5341 ccgtgccagt tggatctcgt ttggctctgc ggcataacgc ccgtagttct gtgtctgttt
     5401 gtcggtcaga aaatcagcag ccatcacgca tcctttcatt atttattgat ccgtattatt
     5461 ttggcggtat cagatattat aaattttgag accaatgaaa actgccctaa acatgagtat
     5521 caaaaatcgt gatttatgcc ttatgatact acgcagtatc aatatcgatt tatgagacca
     5581 aaatggcatt attagggtat gcaagagtat caaccagcca tcagaaactg acgagccagg
     5641 ttactgagct caacacagcg ggagtgaggg atgatcgagt gtttactgac atgatgtcgg
     5701 gagcaactga tgagcgcgaa gggctacagc gactccttgc cagagcggag aaagatgaca
     5761 ttattctctg tacaaaaatg gatcgtcttg gacgtaatac cgcagatatg attcgcatcg
     5821 tggatacttg ctataaaaaa ggcatcgcta ttcgctttct tgagaacggc cttagtactg
     5881 aaggcactat gggaaaaatg gtcatccaga tactagctgc tgttgccgaa gcggaacgtg
     5941 aaaggatctt ggaacgaact aacgatggac ggcaggcagc tatgattaag ggcgtcaaat
     6001 tcggaagaaa acctcataga aaggctgaat tagctaatga attgatcaat aaggacgtag
     6061 aagtaaaaga ggtaatggaa aaaacgggta tatcacgggc gacgtatttt aggctgaaaa
     6121 gaaaaacggt tactaacaat attaatcttt ccaataataa ataagaaaga tgagcattct
     6181 taaaaacctg aacggcttct atttagcaat atttaaactc gaaaaaagga tgtattcaaa
     6241 ttgagtcaat atttaaagct tggcgatgac cagtctccgg ttcagttgga cccacacagc
     6301 gaagttggtg catttaaagt cgttggacac tgcgcatggg caaaaccaga agataaaatc
     6361 actcctgact ttttgcgttc aagagtagaa ccttggttaa ctgcactttt tcagtctgaa
     6421 cacttaaata tacttattgg cgccggcctt agttcggcta tccaagagtc agcgaccggc
     6481 accaaacctc aaggtatggg atggattgaa gatctcaaag tttgtaaggc tgaaatagac
     6541 agttatgtag ctaaaacagc agacgcctca ggaaggggaa agggtaatat tgaagatcag
     6601 attcgttcaa ttaatgagtt gattaaagga ctagagattt taacggtaca aaacctaccg
     6661 gttccaccat ctccaccagg tgctccctca tatcgaaatt taaaaagtga gttagtagag
     6721 ttaaataatg agctaacaag gtgtctgaaa ttattttcag actcagttag ttatggtgaa
     6781 aaattaattc gtgatgcaaa caacgattta aaaacagaaa catttaatta tttagtaagt
     6841 tttttgataa gtttttcaag tcgaacagca actagggatc gattacatat ttttacgaca
     6901 aattacgaca ggataattga aacgggcgct gaaatagcag gtcttcgttt aattgacaga
     6961 tttgtcggta ctatagcacc aatttttaga tcttctcggt tagaagtgga ttatcattac
     7021 aacccaccag ggattcgggg tgagccaaga tatttggaag gtgtggcgcg atttactaaa
     7081 ttacatggtt cattggactg gtatgctact gaaggggcta taaggcgttt tggattgcca
     7141 tttggagcca gttcggttga acccttttta caggtggaaa ctaccggaac tgcaaattat
     7201 caacaattaa tgatttatcc aaattcagtt aaagaccgtg aaacatctga atatccttat
     7261 gttgagctat ttagggattt agcatctgca acatgtcgtc ctaatagcac cttggtaaca
     7321 tatggttata gttttggcga tgagcatata aatcgggtta ttattgacat gttgacaata
     7381 ccctcaacgc atatagtaat aattgcatac ggtgatccac ttggtagaat catgcgtttt
     7441 ataaacgata gtggaagaaa agctcaaata tctttattgc ttggtgatca ctttggagat
     7501 ataaagaaat tagtagattt ttatcttcct aaagccgcga tagacagatc atcaattcgt
     7561 atggcggagc tattgaagtc aagaggtttg tatcgtgcaa ctacctcaaa taatagtggg
     7621 gattccgaat gaactcattt cccattgaac aaggtgagca actcagggta ggtactgtag
     7681 attttgtttc acccaatgaa attagagcac tattagatat cgattcacct gatgctgtcg
     7741 ctttgaatgc aggcacacct agaaattttc ctagagtaaa cagctatgta ttagtctcat
     7801 gtgataatgg ttacttagtt gggcaaatag agtggcttgc agttgagcat tcaccttatc
     7861 ctaaacaaag agatattcaa gattttggtc tagtaaacct accatttcct cgaaaaaaaa
     7921 taagtttaaa tccagtcgga actcttaaac gcttttccaa agatggtact gattatttta
     7981 aatttcaacg cggctctgaa tcatttcctt ctattggttc agctatttta ctaccaacag
     8041 atttacagct tagatcaatt gtcgagtctg gtgataatag acgaatagtt attgggcaaa
     8101 gtccacttgc taacaatgct aatgtagccg tagaccctga tagattattt ggtcgtcata
     8161 tagcagtact tggtaatact ggtagtggta agtcttgttc tgtcgcagga ttaatccaat
     8221 ggtcactcga agctgcaatg gagtcagaag taaaaccaaa cgccagattt attatattag
     8281 accccaatgg cgagtatgta cgtgctttgg gaccaactac aaaatttaaa ggcagaattt
     8341 ttaaagtcga agctggggaa aatgagcatc aactccaagt gccatcttgg ttttggaata
     8401 gctcggaatg ggcatcattt acgcaagcga gtcctaaagc ccaacttcct ttactaaagc
     8461 gttctctaag ggcaatgaga aacgaagatt ttgaactcga aacaacctca aatatagaaa
     8521 taaaacgttt tctagggaat gttctcataa ccttaaaaca tgataaaagt tcagggaaac
     8581 cctttggagc ttgggggcct gcaaaaggct ttaacgagat aatgggtaaa tggattgaaa
     8641 gctttgaagg atacagagat aaacttggaa ataacaactt aacgaatgta atcacaataa
     8701 tgtctaaata tagacaagat aggaatgtac agtacgcaac catgcaagcg accgtgcctg
     8761 aaatagatag cattatcgag agtgttacct ctgcatttag agagttcgga gggtttgaga
     8821 aagagttact accaaagaat gaagatatac cgttaccatt tgatggtaat aacctggttg
     8881 cctatttaga agctcttgct caagaaaatg gcagtgaaca atatgttgaa tatttggttg
     8941 ccagaatacg caccatgctc tcagatacaa ggatgagatc tgttactaat gatgctgata
     9001 atcggattga cttagctaaa tggttagaga aatatcttgg taccgattgt tcaagagatg
     9061 ataacagttg tatttctata atagatctat ctctagttcc aagcgaaatt actcacattg
     9121 ttactgctgt gatttcaagg attatttttg aatcattgca acgctataga agactatata
     9181 ataaatcttt acctacggta cttgtagctg aagaagcaca tacatttatt aaacggtatc
     9241 gtgatgactc tgaaaatcag gatgtcgcag ctgtttgctg ccaagtgttt gagaaaattg
     9301 cacgagaagg cagaaaattt ggtcttggga tggtggtttc ctcgcaacga ccatcagaat
     9361 tatcacctac agttttatct caatgtaata cttttcttct gcatcgaatt agtaatgaca
     9421 aagatcagga gcaagttcat aaaatggtac ctgataattt gcgaggatta ctccgtgagt
     9481 tgccttcact gccatcccaa catgcaatac tgatgggctg ggcatctgag cttcctgtcc
     9541 tggttaaaat gaaaaatttg acgaaagatc agcaacccca ttctgatgac cctgacttct
     9601 gggatgtttg gacaagaaag tatgctgatg ggaaattggt cgagagaact gtcgattggg
     9661 gagctgttgt aaaggaatgg caacaaaagt gatctgcggc agaaaatgaa agcgggaggg
     9721 cagatcggaa ttgaaggaac tttatgtcat caatctactt ggtgttgcgt tccgttgtcc
     9781 ctggggtccg cttggaaaaa ggaaattatc ctacgctaag cgcatttttt gcacagatgg
     9841 ctaccgactg aacgtaacgt gtgagtcatg atcagaaatg tgttaactct aggaattgac
     9901 acgacttact gtttgatggg gaaatcatgt ttagctatgg aaggtgcgaa caagtccctg
     9961 atatgagatc atgtttgtca tctggagcca tggaacaggg ttcatcatga gtcatcaact
    10021 taccttcgcc gacagtgaat tcagcagtaa gcgccgtcag accagaaaag agattttctt
    10081 gtcccgcatg gagcagattc tgccatggca aaacatggtg gaagtcatcg agccgtttta
    10141 acccaaggct ggtaatggcc ggcgacctgg cactgttgca aatagtcggt ggtgataaac
    10201 ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc ggcattttca
    10261 gcgctgcaaa tacggcatca gttaccgtga gctgcaggag atgctggctg aacgcggagt
    10321 gaatgtcgat cactccacga tttaccgctg ggttcagcgt tatgcgcctg aaattgaaaa
    10381 acggctgcgc tggtactggc gtaacccttc cgatctttgc ccgtggcaca tgg
//
