LOCUS       NZ_KM107841             6567 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain G3/10 plasmid pSYM5, complete sequence.
ACCESSION   NZ_KM107841
VERSION     NZ_KM107841.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867340
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Zschuettig,A., Auerbach,C., Eichhorn,C., Blom,J., Goesmann,A.,
            Jarek,M., Scharfe,M., Zimmermann,K., Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Zschuettig,A., Auerbach,C., Meltke,S., Eichhorn,C., Brandt,M.,
            Blom,J., Goesmann,A., Jarek,M., Scharfe,M., Zimmermann,K.,
            Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: GS de novo assembler v. 2.5.3
            Sequencing Technology             :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009963665.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107841.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G3/10"
                     /db_xref="taxon:562"
                     /plasmid="pSYM5"
     gene            join(5982..6567,1..>2)
                     /locus_tag="EL76_RS00005"
                     /pseudo=""
     CDS             join(5982..6567,1..>2)
                     /locus_tag="EL76_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            78..1001
                     /locus_tag="EL76_RS00010"
                     /old_locus_tag="EL76_p7501"
     CDS             78..1001
                     /locus_tag="EL76_RS00010"
                     /old_locus_tag="EL76_p7501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_001118645.1"
                     /translation="MAKQKFRITNWSTYNKALINRGSLTFWLDDEAIQAWYESATPSSR
                     GRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLRCPDYTSVSKRAKSVN
                     VSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRKLHLAVDSNTHEVVCA
                     DLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRRKKISALIPPRKGAGY
                     WPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETAMYRMKQLLGDSLTLRDYDGQ
                     VAEAMAMVRALNRMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            994..4356
                     /locus_tag="EL76_RS00015"
                     /old_locus_tag="EL76_p7502"
     CDS             994..4356
                     /locus_tag="EL76_RS00015"
                     /old_locus_tag="EL76_p7502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="WP_172687137.1"
                     /translation="MPENPASYRVVRTKSYLFNKASIGFNSVYAATPVSCSTTGTSEND
                     LFCGAGANTSGSVKSIAIGNNALVSKNASAGLAVGYNSKSAAQNAIAIGNSAIASAANT
                     ISIGSDNSFTTGSVAIGTNARAGREDILSLKDAGKSERTWIGKQNNIALGVGSVADGGR
                     VISIGENAGSGTIDNWNIQNINIGTNAGSQAKRDYSVALGFEAGMVLSGSQDATEDSKR
                     APSINIGKQAGKNTVSYGNISVGDNAGTEITDNRSVSNIMIGNGAGVGLSSNDGINTTF
                     PGYGPGGNTLVGNASGRQLSGDSNVAVGSIAGDQSTGDNNVYVGHLAGQQSMSDRSIII
                     GSQAGLGTNNDRSVLIGNFANGGVAAPTRNVVGLGSSVKATGFESIAVGFNANSSANNA
                     TAIGRFTTASGVNATALSTNAMASGPNSIAIGNGAKAVAMDTISIGTGNIVSGEKSGAI
                     GDPSTISGSASYSIGNNNKITANNAFILGNAVDNAIDNSVALGNSTTVSSAVATPEYIV
                     NGVTHSFAGSTPVSTVSIGASGKERTLSNLAAGRISATSTDAINGSQLFAVSSEVDKGN
                     LFAGNSGAFKRFFGETTTIRGGLTNGETASNKNIRTEANDGIIDIQLANNLDVTSVKTG
                     NTLINNSGLYITGGPTVTVTGIDAGNKTITNVADAVNDSDAVNKRQLDNLSNSVSQGVT
                     FSANEGDDIKRKSGEVLALKGDGAVNGNYSGKNIKTVADASSGVISIQISESPVFGNVV
                     INSNSSGKISGVSDGAIAMGSSDVVNGGQVHTVTTSIANVIGGDTYVSHNGEMISSNVG
                     NTGKNTIHDAIDSIRTTAESVSGGWNLSVNGKQESAIKPSETVDLNNTDKNISISKKDN
                     QVSFNLSDSVNVKESISVENGPSLSQVGIDGAGLKIANIADGIIAQGSKDAVNGGQLHE
                     FISSEVTKPITFKADSGTPYEAKMGSLVNVKGDNKNIKTTVNGNVLSINLNDNVNVKSL
                     TTTESLAVSSGANIDMGGNTIQNVGNAKRTSDAVNYGQLQQAFSSLDNQINRVERRANA
                     GAAAAIATAGLTQAYIPGKSMMSMSGGSFQGENSLAIGLSTISDNGNWILKGSYSTTTR
                     SQTGASVGVGFQF"
                     /name="YadA-like family protein"
     gene            4411..4845
                     /gene="bamE"
                     /locus_tag="EL76_RS00020"
                     /old_locus_tag="EL76_p7503"
     CDS             4411..4845
                     /gene="bamE"
                     /locus_tag="EL76_RS00020"
                     /old_locus_tag="EL76_p7503"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="WP_115259208.1"
                     /translation="MKSNSFKIIIFIACATIISGCNQNMSRPDRDGGVKEIVWPEPVNA
                     KLRSGRGVFPTSESISLLNKGMTKDQVYLLIGRPHFGEGLFSVREWDYLLHFNTLKHTN
                     NQVTICQLKIIFNSDKLVANIYWRAIKPENSICPPESNKN"
                     /name="bamE"
     gene            4945..5430
                     /locus_tag="EL76_RS00035"
     CDS             4945..5430
                     /locus_tag="EL76_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_239944593.1"
                     /translation="MKRNITILSLLVILFTLSACHQKNVKSVVTPLTPQINKEVSSLIL
                     LPLSEQPHNGWAEIIIVDRKWYFDTNTVLTRNELASMSFGQSNEGNWFLQISPNKNGQL
                     IIDKALENKNNYILMVLNGKAISLSQLNSAKTLSFYVGDENKTIQIAEAITQKNIMR"
                     /name="hypothetical protein"
     gene            complement(5602..>5949)
                     /locus_tag="EL76_RS00030"
                     /old_locus_tag="EL76_p7504"
                     /pseudo=""
     CDS             complement(5602..>5949)
                     /locus_tag="EL76_RS00030"
                     /old_locus_tag="EL76_p7504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
ORIGIN
        1 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcctcccg
       61 acaacacaga ccattccgtg gcaaagcaaa agttcagaat caccaactgg tccacctaca
      121 acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatgaa gcgattcagg
      181 cctggtatga gtcggcaacg ccttcatcac gaggaaggcc ccagcgctat tctgatctcg
      241 ccatcaccac cgttctggtg attaaacgcg tattccggct gaccctgcgg gctgcgcagg
      301 gttttattga ttccattttt gccctgatga acgttccgtt gcgctgcccg gattacacca
      361 gtgtcagtaa gcgggcaaag tcggttaatg tcagtttcaa aacgtccacc cggggtgaaa
      421 tcgcacacct ggtgattgat tccaccgggc tgaaggtctt tggtgaaggc gaatggaaag
      481 tcagaaagca cggcaaagag cgccgtcgta tctggcgaaa gttgcatctt gctgttgaca
      541 gcaacacaca tgaagttgtc tgtgcagacc tgtcgctgaa taacgtcacg gactcagaag
      601 ccttcccggg ccttatccgg cagactcaca gaaaaatcag ggcagccgcg gcagacgggg
      661 cttacgatac ccggctctgt cacgatgaac tgcgccgcaa aaaaatcagc gcgcttattc
      721 ctccccgaaa aggagcaggt tactggcccg gtgagtacgc agaccgcaac cgtgccgttg
      781 ctaatcagcg gctgagcgga agcaatgcac ggtggaaatg gacaacggaa tataaccgtc
      841 gctcgatagc ggaaacggca atgtacagaa tgaagcagtt gttgggagat tcactgacgc
      901 tgcgtgacta cgatggtcag gtagcggaag ctatggccat ggtgcgtgcg ttgaacagga
      961 tgacaaaggc tgggatgcca gaaagcgtgc gtattgcctg aaaatccagc cagctacagg
     1021 gtcgttcgca cgaaatctta tttattcaac aaagcctcaa taggatttaa ttcagtttac
     1081 gcagctacac ctgtttcctg tagtactaca ggtacaagtg aaaatgacct gttttgtggt
     1141 gcaggggcca atactagtgg ttccgtgaag agtatagcga tcgggaacaa cgcactagtg
     1201 agtaaaaatg ctagtgctgg gttagcagtt ggttataact caaaatctgc tgcacaaaat
     1261 gctattgcga tagggaatag tgcaattgca tcggctgcaa atactatcag tattggttcc
     1321 gacaactcat ttacaactgg atctgtggca ataggtacaa atgcacgtgc agggcgagaa
     1381 gatatcttaa gcctaaaaga tgctggtaag tctgagagaa cctggattgg aaaacaaaat
     1441 aatattgccc ttggagtagg ttccgtagct gatggtggtc gagtgatctc tatcggtgag
     1501 aatgcaggaa gcgggactat tgataactgg aatattcaga atatcaacat aggtactaat
     1561 gctggaagtc aggcaaaaag agattatagt gttgctttgg gttttgaagc agggatggtg
     1621 ctatctggct ctcaagatgc taccgaagat agcaaacgag ctccaagtat taatattggt
     1681 aagcaggcag gaaaaaacac agtatcatat ggaaatatat ctgtaggaga taatgctgga
     1741 acagaaatca cagataatag aagcgtttct aatataatga ttggaaacgg ggctggagtt
     1801 ggtttgagtt ctaatgatgg tataaacact acttttccag gatatggccc tggtggaaat
     1861 accttagtcg ggaatgcttc gggacgacaa ctatctggtg atagtaatgt cgctgtaggt
     1921 tctattgctg gcgatcagtc aactggtgat aataatgtgt atgttggcca cttagctggg
     1981 caacaatcaa tgtcagatcg atcaattatt ataggttctc aggcaggatt aggaacaaat
     2041 aatgacagaa gtgtattaat cggtaatttt gccaatggag gcgttgctgc tccaacacga
     2101 aatgtagtgg ggcttgggtc ttctgttaag gctacggggt ttgaaagtat tgctgtgggt
     2161 tttaatgcaa attcatcggc aaataatgca acagctatag gtcgctttac cacggcttct
     2221 ggggtcaatg ctacagctct aagcactaat gctatggctt cgggacctaa ttcgatagcg
     2281 ataggtaacg gtgcaaaagc agttgctatg gatactatca gtataggtac aggaaacatc
     2341 gtaagtggtg agaaatcggg tgcaataggt gacccaagca ctatatcagg gtccgcttct
     2401 tattcaattg gtaataacaa taaaataacg gcaaataatg cttttatcct tggaaatgca
     2461 gtagataatg ctatagataa ttcagttgcc ctaggtaatt ctacgacagt atcatctgct
     2521 gttgcaactc cagaatatat agtaaatggg gtgacacact catttgcagg ctcaactcca
     2581 gtatcaactg taagtattgg tgcttctgga aaagagcgca ctctatcgaa tcttgcggct
     2641 ggacgaattt cggctactag cactgatgcc ataaatggta gtcagctatt tgcagtttca
     2701 tctgaagtag ataagggcaa cttatttgct ggtaattctg gagcgtttaa acgcttcttt
     2761 ggtgaaacaa ctactatccg cggaggtctt acaaatggag aaactgcaag taacaaaaac
     2821 attagaactg aagctaatga cggtataata gatattcaac tggcgaataa tctagatgtc
     2881 actagcgtta agactggtaa tacattaatt aacaactcag ggctttatat tacaggtgga
     2941 cccactgtca cagttacagg tatagatgct ggaaataaaa ctatcactaa tgttgcagat
     3001 gctgtaaatg acagtgatgc ggtcaataaa cgtcagttag ataatctttc aaattcagtt
     3061 agccaagggg taacgttttc tgctaatgaa ggcgatgata tcaaacgtaa atctggtgag
     3121 gttttagctc tgaaaggaga tggtgcagtt aatggtaatt atagtggaaa aaacattaaa
     3181 acagttgcgg atgcatcctc cggagttata tcaattcaaa tatctgaaag tccagtattt
     3241 ggcaatgttg ttataaatag caatagcagt gggaaaattt ctggcgttag tgacggtgcc
     3301 atcgctatgg gcagtagcga tgtcgtgaat ggtggacaag ttcatacagt tactacatca
     3361 attgccaatg ttattggagg tgatacttat gtaagccata atggtgagat gatctccagc
     3421 aatgtaggta atacagggaa aaatactatt catgatgcta ttgattccat cagaacgact
     3481 gctgaatcag taagcggagg ttggaacctg tcagtaaatg gtaaacaaga aagtgctata
     3541 aaaccttctg aaacagttga ccttaataat actgataaaa atatcagcat atccaaaaaa
     3601 gataatcagg ttagttttaa cctttccgat tctgtcaacg ttaaagagag catcagtgtt
     3661 gaaaatggcc cttctctgag ccaagttggt attgatggtg ccggattaaa gattgctaat
     3721 atagctgatg gtattattgc tcaggggagt aaagatgctg tcaatggtgg gcaactccat
     3781 gagtttatca gcagtgaagt aactaaacca attacgttta aggcagatag tggtacaccg
     3841 tatgaagcaa aaatgggaag tttggtaaat gttaaaggtg ataataaaaa tatcaagacg
     3901 acagttaatg gtaatgtgct gagcattaat ctaaacgaca atgttaatgt taaatcatta
     3961 actactacag agtctttagc tgtctcaagt ggtgctaata ttgatatggg aggaaatacc
     4021 atacaaaatg ttggtaatgc caagcgtaca agtgatgctg ttaactacgg acagctacaa
     4081 caggctttca gtagtcttga caaccaaatt aatcgggttg aacgtagagc taacgcaggt
     4141 gcagctgctg ctattgcaac ggcggggtta acacaggcgt atattccagg taaaagtatg
     4201 atgtccatga gcggtgggtc attccaaggt gaaaacagtt tagctattgg cctttcaacc
     4261 atctcagata acggcaattg gatattaaaa ggttcttatt caacaacaac tagaagtcaa
     4321 actggagcat ctgttggagt aggatttcag ttctgataaa acaggggtgg aaacacccct
     4381 ataaataaaa tttgatttgg atagcaaata atgaaatcga actcattcaa aattataatt
     4441 tttattgcat gtgctacaat tatttcagga tgtaatcaaa acatgagtcg cccagatcgt
     4501 gatggtggag ttaaagagat agtgtggcca gaacctgtaa acgcgaagtt aagatcagga
     4561 cgtggagtat tcccgacatc cgaaagtatt tcgcttttga ataaagggat gaccaaagat
     4621 caggtctatt tgcttatcgg taggccgcat tttggtgaag gtctattttc tgtgagagaa
     4681 tgggattatc tccttcactt caatacgtta aaacacacta acaatcaagt tacaatttgt
     4741 caacttaaaa ttatttttaa ctcagataaa ttagtcgcta atatatactg gagagctatt
     4801 aaacctgaaa attccatttg cccaccagaa tctaataaaa attaaaataa tcaatatagg
     4861 aaagtaaatg gtgtaacttt tataaacata aggttttgtt gctgtacagt aatagatagt
     4921 ataaagcaat ttcaaggtaa atatatgaaa agaaatatca caatcctaag cctattagtt
     4981 attttattca ctctgagcgc ctgtcatcag aagaatgtta aaagtgttgt aactcctctc
     5041 accccccaaa taaataaaga ggtatcttct cttatacttc ttcctctttc tgagcagccg
     5101 cacaatggat gggctgaaat tatcatcgtc gacaggaaat ggtatttcga tacaaatact
     5161 gttctgactc ggaatgaact tgcgtccatg tcatttggtc aaagtaatga aggtaattgg
     5221 tttctacaga tcagtcctaa caagaatgga caattgataa ttgataaagc attagaaaat
     5281 aagaataatt atattcttat ggttttaaac ggaaaggcta tctcactttc gcaactcaat
     5341 tcagcaaaga cattatcgtt ttatgttggt gatgagaata aaacaattca gatcgcggag
     5401 gctataactc aaaaaaacat tatgcgctag tatttatttt actgattttt taagataatt
     5461 tcgatggtca tttagaggca gtgtcttgtt ttcttgtggc cataatattt acttggtggt
     5521 atttatggaa aagttacgcc aagcgctttg gcactgttgc aaagttagcg atgaggcagc
     5581 cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc gcatttcgcc
     5641 caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac gcatcacctc
     5701 aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg tggcgccgat
     5761 tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact taatctgtcg
     5821 gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg cgcgaccata
     5881 ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca cgttgttgag
     5941 gattttaccg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc
     6001 atcctcccga caacacagac cattccgtgg caaagcaaaa gttcagaatc accaactggt
     6061 ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgaag
     6121 cgattcaggc ctggtatgag tcggcaacgc cttcatcacg aggaaggccc cagcgctatt
     6181 ctgatctcgc catcaccacc gttctggtga ttaaacgcgt attccggctg accctgcggg
     6241 ctgcgcaggg ttttattgat tccatttttg ccctgatgaa cgttccgttg cgctgcccgg
     6301 attacaccag tgtcagtaag cgggcaaagt cggttaatgt cagtttcaaa acgtccaccc
     6361 ggggtgaaat cgcacacctg gtgattgatt ccaccgggct gaaggtcttt ggtgaaggcg
     6421 aatggaaagt cagaaagcac ggcaaagagc gccgtcgtat ctggcgaaag ttgcatcttg
     6481 ctgttgacag caacacacat gaagttgtct gtgcagacct gtcgctgaat aacgtcacgg
     6541 actcagaagc cttcccgggc cttatcc
//
