LOCUS       NZ_KM107838             4197 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain G3/10 plasmid pSYM2, complete sequence.
ACCESSION   NZ_KM107838
VERSION     NZ_KM107838.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02867340
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Zschuettig,A., Auerbach,C., Eichhorn,C., Blom,J., Goesmann,A.,
            Jarek,M., Scharfe,M., Zimmermann,K., Wassenaar,T.M. and Gunzer,F.
  TITLE     Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and
            draft sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Zschuettig,A., Auerbach,C., Eichhorn,C., Blom,J., Goesmann,A.,
            Jarek,M., Scharfe,M., Zimmermann,K., Wassenaar,T.M. and Gunzer,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2014) Institute of Medical Microbiology and
            Hygiene, TU Dresden, Medical Faculty 'Carl Gustav Carus',
            Fetscherstrasse 74, Dresden, Saxony 01307, Germany
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: GS de novo assembler v. 2.5.3
            Sequencing Technology        :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009963635.1-RS_2024_06_24
            Annotation Date              :: 06/24/2024 03:32:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM107838.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G3/10"
                     /db_xref="taxon:562"
                     /plasmid="pSYM2"
     gene            complement(55..1011)
                     /locus_tag="EL76_RS00005"
                     /old_locus_tag="EL76_p6001"
     CDS             complement(55..1011)
                     /locus_tag="EL76_RS00005"
                     /old_locus_tag="EL76_p6001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_223152166.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVHKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQQSILKQIEKYSDFPYVVNYYRNRLSALVD"
                     /name="type II restriction endonuclease"
     gene            1082..2221
                     /gene="dcm"
                     /locus_tag="EL76_RS00010"
                     /old_locus_tag="EL76_p6002"
     CDS             1082..2221
                     /gene="dcm"
                     /locus_tag="EL76_RS00010"
                     /old_locus_tag="EL76_p6002"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_000131512.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            3040..3234
                     /locus_tag="EL76_RS00015"
                     /old_locus_tag="EL76_p6003"
     CDS             3040..3234
                     /locus_tag="EL76_RS00015"
                     /old_locus_tag="EL76_p6003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3899..4060
                     /locus_tag="EL76_RS00020"
     CDS             3899..4060
                     /locus_tag="EL76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     Typing          complement(2526..2781)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.899"
                     /coverage="89.0"
                     /evalue="2.61e-118"
     Typing          complement(2906..3004)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(3318..3402)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          3426..3971
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.989"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caggtccaag attacgcgca gaaaaaaagg atctcaagag atccttttag ttttttagtc
       61 aacaagagca ctaagcctat ttctataata attaacaaca taagggaaat cactatattt
      121 ttctatttgt tttaatattg actgttgaat ttcctctttt tcagagtctg tgtaacttac
      181 attattccac tttcttgata attccattgc tgactgtagc atatcttcaa aggttagcac
      241 cctattatta tttttatatt tgaaattttt attttctatc gtcgttacaa cgaccacatt
      301 agcctcgtat aatatattta ttgtctcctc gctaaaagaa tcatctagtg tagctagata
      361 catttctcta attcctgttc tattaacctc ttcaggtact tcttgccatc gctccctcag
      421 cgttgtttta gcggaaatca acattgtatt tcttttgtta gatgtgtact ggactacacc
      481 tggcataaca agatctacaa gtttacctat ttggtttttt tgaaaaaatg acttcccaat
      541 agccccctgc acatcaacag gaattcctgc ccccatcatc aatagctcaa gaatagactc
      601 aaactctttt cctgccctgc tacgtctgga ttgagtgtta gacagtgcca aatcatatat
      661 gtgtgtaggg tattccatcg taaaatcacg aatggcatca taaggactca tatcttttat
      721 tctttcttca tcaataagat atgataacat tcttgttgtg aattcttttt caaatggttc
      781 aaattctttc caacagtttt tcctcattgc aattatatat tcacttggat tattcaaaaa
      841 ataatcacta tttttttgat cataccctaa atcacaaaat gttttatgaa ccaaagcaaa
      901 tggcgtaata aaatgcgact ttctctcttt agaaattaga gttttaaatt caccaggact
      961 caatctttgc ataaaaaata accttttata tctacttgaa agttttttca tgcacgttca
     1021 taattggaat caaaacagga caaattgtcc taaaaccaac acttaattct ggtagacatg
     1081 catgactgat aatatcgcag caacaatcaa aggaaaaaga gaaagacttc atatgactca
     1141 aaaagagttt gcagacgccc ttggtctttc aaaatatgga gacagaacca taagaagatg
     1201 ggagcgcggg gaaacaaaac ctactggggc ggaactaaaa gcagttatgg actttccaga
     1261 cacaccacct tacccaaaca acgagaatgg cagatatcgc atgatcgatt tatttgctgg
     1321 aatcggtgga accagacttg gatttcatca aactaacgct gtaaatgttg ttttttccag
     1381 cgaatgggat aagtttgctc aaaagacata tcatgcaaat tatggtgatt tccctgatgg
     1441 ggacataaca aaaatcgatg aaaaagatat tcctgatcat gaaattcttg tcggtgggtt
     1501 tccttgcgta gcattctctc aagcagggct caaaaaagga tttaatgata caagaggaac
     1561 tttgtttttt gatatagcaa ggataataaa ggaaaaaaaa cctcatgctt ttttacttga
     1621 aaatgtaaaa aacttgctag gacatgataa aggtagaact ttttcaataa taaaaaacac
     1681 acttgaagaa ttgaattaca ctgtgtatta caatatcttc gctgcaaaag actttggtgt
     1741 tccacaaaat agagaacgca tatacattgt aggctttaat aaagaaaaag taagaaatca
     1801 tgaacatttt acttttccaa ccccattaaa aacaaaaaca agagttggag atattcttga
     1861 aaagagcgta gacaataaat atacgctatc agatgcgctc tggaatggac accaacgcag
     1921 gaaacttgta aatgcagcag ccggtaaagg ctttgggtat ggtttattta atgaaaatag
     1981 cccatatact aataccataa gtgcgaggta ctataaagat ggtagcgaaa tattaataga
     2041 gcaaaaagga agtaacccaa gaaaaataac tccgcgggaa gcgtcaagac ttcaagggtt
     2101 tcctagtgat tttattatcc ctgtatcgga tacacaggct tataagcaat ttgggaattc
     2161 agtcgcagtc cctgttatca atgcaatagc agaaaaaatc atatcgacct tagattcata
     2221 aactaagtca taaggacaca atgcggccca gaatccaatt ccccccccca tatggattta
     2281 ccataacgtc gcattgcgcg caccgctaaa acgcgctgaa agcgattctg ccgcgaacac
     2341 cgcaaaaaga gatgttctcc ccggggtaaa ccggaaaaat gcgtcaggat cgctttcagc
     2401 tcactgcata gctatgcatg aaagtgaatg acgatcggtt tgggcgcata cagtgcccat
     2461 gagacacatt ttcggacatt ttcagttcgt gaatccctga ctcattgcta catatctgaa
     2521 cagacacccc tagcagcgcc cccaggggtg tcctgtaaaa aaacacaccg cgccgctagc
     2581 agcaccccta atataagatg atgtttttta tgaaaatagt cagtaccacc cctaaaaagc
     2641 ggtgttggcg cagctacaac accgcgccga caccgctttt ttaaatatca caaagagagc
     2701 aagaagaact aatttgtcat aactctctat ttataaagaa aaatcagcaa aaacttgttt
     2761 ttgcgtgggg tgtgggcatt tcaggcatga gaacagactc gcgtttttcc ggagtatcag
     2821 cggaattatt gattaagaaa cactcgacgc agagcgaaat tttatgcaac agcggctgca
     2881 ctgcacaggg attatttatc agttgatatg ctgtgccatg atacgtccta aagaaacagg
     2941 acaggatgtg caattgtaat accgtcgccc gcaacgtctt acaattacca cctgctcagg
     3001 gtccccccga cacccggaac tgacggagaa caaaggaagg tgaacaaaca acaacaaact
     3061 gcgctgaata tggcgcgatt tatcaaaagc cagagcctga cactgcttga aaaactggat
     3121 gctctggatg ccgacgaaca ggcggccatg tgcgaacgac tgcacgaact cgcagaagaa
     3181 ctccagaaca gcatccagat tcgctttgag gccgaaagtg aaacaggaac ataacgaagc
     3241 tgacggaggc ggtcacggct ttcccgtggg cgttttcgtc agcagacaag cccgtcaggg
     3301 cgcgtcaggg ggttttagcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     3361 cggagtgtat actggcttaa ctatgcggca tcagtgcgga ttgtatgaaa agcacgccat
     3421 gccgggtgtg aaatgccgca cagatgcgta aggagaacat accgcatcag gcgctcttcc
     3481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcaagc ggtgccggct
     3541 cactcaaagg cggaaatacg gttatccaca gaatcagggg ataaggccgg aaagaacatg
     3601 tgcgtaaaaa ggcaaagggc aacaaaaggc cgcgccgctg gcgtttttcc ataggctccg
     3661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag gggtggcgaa acccgacagg
     3721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3841 tagctcacgc tgttggtatc tcggcccggt gtaggtcgtt cgctccaagc tgggctgtgt
     3901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3961 caacccagta agacacgcct taacgccact ggcagcagcc actggtaacc ggattagcag
     4021 agcgagattt atccagatgc ttacagagtt cttgaagtga tggcctaact acggctacac
     4081 tggaaggaca gtatttggta tctgtgctct gctgaagcca gattacctcg gttagataat
     4141 ctgccaggat tagctaacct tcgaaaaaac cacctgccag ggtggttttt tcgttta
//
