LOCUS       NZ_KM085453             3173 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Escherichia coli O104:H7 strain RM9387 plasmid pO104:H7_S3, complete
            sequence.
ACCESSION   NZ_KM085453
VERSION     NZ_KM085453.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226454
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H7
  ORGANISM  Escherichia coli O104:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Yan,X., Fratamico,P.M., Bono,J.L., Baranzoni,G.M. and Chen,C.Y.
  TITLE     Genome sequencing and comparative genomics provides insights on the
            evolutionary dynamics and pathogenic potential of different
            H-serotypes of Shiga toxin-producing Escherichia coli O104
  JOURNAL   BMC Microbiol 15, 83 (2015)
   PUBMED   25887577
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Yan,X., Fratamico,P.M., Bono,J.L. and Baranzoni,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method :: Celera Assembler v. 6.1; CLC Genomics Workbench
            v. 7.0
            Coverage                     :: >120X
            Sequencing Technology        :: IonTorrent; Pacific Biosciences
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009963625.1-RS_2024_05_16
            Annotation Date              :: 05/16/2024 01:42:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KM085453.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli O104:H7"
                     /mol_type="genomic DNA"
                     /strain="RM9387"
                     /serotype="O104:H7"
                     /host="cattle"
                     /db_xref="taxon:1619910"
                     /plasmid="pO104:H7_S3"
     gene            complement(95..250)
                     /locus_tag="HTD06_RS00005"
     CDS             complement(95..250)
                     /locus_tag="HTD06_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(801..1229)
                     /locus_tag="HTD06_RS00010"
     CDS             complement(801..1229)
                     /locus_tag="HTD06_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004029842.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEMYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            1521..1949
                     /locus_tag="HTD06_RS00015"
     CDS             1521..1949
                     /locus_tag="HTD06_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2338..2661)
                     /gene="mobA"
                     /locus_tag="HTD06_RS00020"
     CDS             complement(2338..2661)
                     /gene="mobA"
                     /locus_tag="HTD06_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_087614075.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQEKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2945..3139
                     /locus_tag="HTD06_RS00025"
     CDS             2945..3139
                     /locus_tag="HTD06_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2671..2907)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          2..526
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.717"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(2342..2661)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="8.31e-62"
ORIGIN
        1 gcgtaaggag aaaatgtcac ataaggcgct cttccgcttc ctcgctcact gactcgctac
       61 gctcggtcgt tcgactgcgg cgagcggtgt aagctcactc aaaagcggta atacggttat
      121 ccacagaatc aggggataaa gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag
      181 aagccagcgc cgcaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gccagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcggt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gaatccaacc cggtaagaca cgacttaacg
      541 ccactggcaa cggccactgg taagcaggat gtgcgagaga tgtaacgaga gttcttgaag
      601 tggcggccta actgaggcta cactgaaagg acagtatttg gtgacatgtc tcgcacaaga
      661 ctgttaccac ggttagagaa tctgccagga ttaactaacc ttcgaaaaac cacctgccag
      721 ggtggttttt tcgtttttac aatagtgaga ttacgacgag actgaatgaa tctcaagatt
      781 tcatttagcc tgccgctcga tcattcaaag ttttgaggta gcttcattgg gtcatggtat
      841 ccaagctcta aaagctttat atggttcggt gtcagccgct ggaaatgaat aacccagtca
      901 gaaaccttgt atttcccatg aaacgtatct ttaaaaaccg gatcgcctaa atgagcatgc
      961 cataatttgt tagctctagc atacatctca atagcttctt tgttttcgta atgatcaggc
     1021 actttccacg atggcgatac tttgccagtc caagctggat atccttttaa aggatcgcca
     1081 aagagattgt atttttcaaa atgttcaatg aaatcatcga ttttatcgag tgtttttgca
     1141 gaatatctgg atgtgtaata aaggttgaag gtctcagaga atgagacaat attcaattgt
     1201 ggattttgcg cgcctgctcc gctgcccatg ctcttctctg ctcccgactt agaccagatg
     1261 gcattgtaac agacttctca ttcgctctgg aaatcaacag agacaaggat acgccaaaaa
     1321 cctcaatatc tttaacaaga tcatgacgtt gttcggtttt catatcgcta actccgtatc
     1381 taattcaggc aggaaaaaat cttttcctcg ttttcagagt agtctttttt ggttgcacaa
     1441 taatggcgcc agtgtcaccc tattcatatg aatataccat ataatatata ctttaaatat
     1501 tttggggctt aggaaaatca atgacttact ccgttaagaa taaatggaaa gctggcggtt
     1561 tgtctcttgg ctggacggca gtgcctaccg tattgttttt tatacaaaac gagaaaaagc
     1621 taacttcagt ggcctttaac acactactta acctgatcgt tcactggtgg agtttgcaag
     1681 agtggccgca tccgtcaatg gaaagtctag ccatacgtat gggggtttct gtacggactg
     1741 ttcagagagc tatcaatgat cttgaaaaag ctaatctctt ggacaagaaa ccaacatcga
     1801 agagtgatcg ccgatatggt ggtcgcaata tttatgatct aacaaagcta gttgactacc
     1861 tagatacaat gggtccatca gtagctgaac aagttaagaa accacgccat aaaaaaccgg
     1921 tatataccgt aagaaaaacg acagcttaaa aaagcacaat ggaactgagc gcgagcgatg
     1981 cacgatagca aaacgatata ttacttattg cattggtctt ggtagccctg cttctggcta
     2041 aaccactgtc aggagtaatt ggctttgcct gtaatgggtt catcgcttca cagttaaagt
     2101 tatcgcatag ctcatatatt taccataatc ccttaattgt acgcaccact gaaacgcgct
     2161 gaacgcgatt ctgacgcaga caccgcaaaa agagatgttt taccccggga aaacaggaag
     2221 gatgcgtcag gatcgttttc agctcgctgc atagctatgc atgaaagcga atggtgatca
     2281 ctttgggagc ttacggtgtt cataccgtct gttttcgaca gttttctctc cgggaagcta
     2341 atctgccata agcctggata acagggcacg gtgatactcc gtaatagcga tcagcacctc
     2401 cgcatattcc ctgtccccga cacgcttgcc gtcgataaag agtttttcct gtaacgcccc
     2461 cagtctcata acttctttca gtacccgatc atcagtcagt gagttaactt tcttaccgag
     2521 agctgccgcc cgtaaaaaac cggaaacggt cttgccgcat tcagcagccc tttttctgat
     2581 ttcctggtct tcttctgccg tcagacgaac agggcgactg atagcccgcc tgcgcgtatt
     2641 acttccactt cgttttgtca taaaacatgg ctccgtatct gacatgggtg tcggggcaaa
     2701 gccctgacca gggtaattgt aatagcgtgc atgcatgcgc ggtataacaa ttacacatcc
     2761 tgtcctgtca gcaagttcga attaccgaca aagacacggc agttttttgg cagtgacagt
     2821 attgtcaggc agaaataccc gtcaaagcgt cagacggcag aataccggcg gtaaaacagt
     2881 cgaaaagtgg tacgcgatat aatcacacaa cgcagatgac agacagaacg gagaaaacga
     2941 aaagatgaac aagcagcagc aagccgtact caacatggca ggattcataa aaagccagag
     3001 cctgacgctg ctcgaaaaac tggacgcact cgatgctgac gaacaggccg ccatgtgtga
     3061 gaagctgcac gaactcgcgg aagaacttca gaacagcata cagacacgct ttgaagcgga
     3121 aaatcgcacc gggatataac gcatccggac aggatgcaga acggcataga gga
//
