LOCUS       NZ_KJ534568             8840 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter baumannii strain ATCC 223 plasmid AbATCC223, complete
            sequence.
ACCESSION   NZ_KJ534568
VERSION     NZ_KJ534568.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226773
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Mosqueda,N., Gato,E., Roca,I., Lopez,M., de Alegria,C.R., Fernandez
            Cuenca,F., Martinez-Martinez,L., Pachon,J., Cisneros,J.M.,
            Rodriguez-Bano,J., Pascual,A., Vila,J., Bou,G. and Tomas,M.
  CONSRTM   GEIH-GEMARA (SEIMC) and REIPI
  TITLE     Characterization of plasmids carrying the blaOXA-24/40 carbapenemase
            gene and the genes encoding the AbkA/AbkB proteins of a
            toxin/antitoxin system
  JOURNAL   J Antimicrob Chemother 69 (10), 2629-2633 (2014)
   PUBMED   24879663
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Gato,E., Tomas,M. and Bou,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2014) Microbiologia, CHU A Coruna, As Xubias 84, A
            Coruna 15006, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.7
            Sequencing Technology             :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 05:22:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KJ534568.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="ATCC 223"
                     /culture_collection="ATCC:223"
                     /db_xref="taxon:470"
                     /plasmid="AbATCC223"
     gene            complement(222..682)
                     /locus_tag="HPF00_RS00005"
                     /pseudo=""
     CDS             complement(222..682)
                     /locus_tag="HPF00_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /name="DIP1984 family protein"
     gene            complement(987..3398)
                     /gene="znuD2"
                     /locus_tag="HPF00_RS00010"
     CDS             complement(987..3398)
                     /gene="znuD2"
                     /locus_tag="HPF00_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_012754356.1"
                     /translation="MLNKSKLFLALITLGASKIVLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(3525..3839)
                     /locus_tag="HPF00_RS00015"
     CDS             complement(3525..3839)
                     /locus_tag="HPF00_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_061855757.1"
                     /translation="MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(3826..4113)
                     /locus_tag="HPF00_RS00020"
     CDS             complement(3826..4113)
                     /locus_tag="HPF00_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            4364..5191
                     /locus_tag="HPF00_RS00025"
     CDS             4364..5191
                     /locus_tag="HPF00_RS00025"
                     /EC_number="3.5.2.6"
                     /function="hydrolysis of beta-lactams"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /protein_id="WP_012754353.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MGVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
     gene            complement(5374..5517)
                     /locus_tag="HPF00_RS00030"
     CDS             complement(5374..5517)
                     /locus_tag="HPF00_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(5529..6104)
                     /locus_tag="HPF00_RS00035"
     CDS             complement(5529..6104)
                     /locus_tag="HPF00_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_012754352.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTPQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATASPQQDATACDTDMQQHATPQQDNDNKKNHELNIAENVAMPQQENTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6097..7047)
                     /gene="repM"
                     /locus_tag="HPF00_RS00040"
     CDS             complement(6097..7047)
                     /gene="repM"
                     /locus_tag="HPF00_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208776.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIEVSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHLFANKLSELPEMGRYSQGTESYPQFAI
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            7670..7924
                     /locus_tag="HPF00_RS00045"
     CDS             7670..7924
                     /locus_tag="HPF00_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014301.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMKPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            8195..8566
                     /locus_tag="HPF00_RS00050"
     CDS             8195..8566
                     /locus_tag="HPF00_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504219.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWTLMKNGASKDEV
                     RMMQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            8566..8739
                     /locus_tag="HPF00_RS00055"
     CDS             8566..8739
                     /locus_tag="HPF00_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282482.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQD"
                     /name="hypothetical protein"
     ARG             4365..5188
                     /name="blaOXA-24"
                     /gene="blaOXA-24"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6098..7047)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.264"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaggtgaaca tactgtcagt acatggtagt tataagtaac gtgtaacttt gtttgcacaa
       61 agatttgagt tatgccaagt gatggattga tttgtactta aaattttaag cttatccact
      121 tgctttgcaa gccactacac tttatttaca atcctatgtt gtggaagtaa gggatcttgt
      181 actcgcccag tatgttcaac ttgattatat ctaaaggact attaggcttc aatcaaatca
      241 attttccaat tagtcgactg aattaacacg tttaaatcac gtaatttaag actaatatca
      301 tcagcttgtt tttgtaatga tgaaactgga ataactttat gccactttat ttcccgcgta
      361 ctataacgat cagattcacg agatgtatta cttattgctg cagttagggt tttgtgctgt
      421 tctacaaatt catcacgcaa agtcaataaa gcaagtagag aacggccatc atttaatttt
      481 gttaaagcat tagtttcatg aattgcataa cttaattttt gaaattcttg aattaaagaa
      541 aatacttgtt ttagtagttc atttggatct tcagatggtt gatcaccatc ttgtaccaat
      601 acatttgcat tgatacgttg ttttagtgaa ataatttttt ttgttgatca cttcgaagta
      661 agagagcttc tgctaatttc atttttcacc tattaaccaa ttagaattaa gttttaaaca
      721 gcttatatcc tcattcttta aagatcaaaa aaattgcttt ttatctagct aaaaggtgtc
      781 tacaaaatcg gtggctattc agtgctgaaa aggctgccga cacataaagt gacccagatt
      841 gaagagttac tgccacactg ctggaaacct aaatcgaatt aaaaaatagg tatgggattc
      901 agcggacgct taccttaatt attaatctaa tcattttata agaaaaatct tctaagaaat
      961 tttcttagaa gattacagta tcttatctag aaattaagat ttaagcctaa actaaaattt
     1021 cttccaattt ggggaatata tggcaaaaat gtttcatggg catagacttt ctgatcaagt
     1081 aaattattcg ctttaaagaa taaatcatat tctgtgtgag atagcttatt tttatatgct
     1141 aaagtcatat taagcatatt ataacctgat gtaacctgct caaatttaga aatattatct
     1201 tgtttaaaga ctcgataata ttctatttca cctttaagcg attcatcaaa atcagctttt
     1261 agtcgtgtac ccaaacgagc aggaggtaat ctaggagtgt aacgatcctt ttggggagct
     1321 aaagtcggtt ctctattcca gatatcgtat gcaattacag catcaggtaa atttgttaaa
     1381 cggcctttca cataatctcc aaaaagagag ccgtgataaa cagaattaaa ttgataccct
     1441 atgttgccct ctaatccata aaaattagca gcagactggc tataatgatt aatacgtagt
     1501 gtatgctgat ctctaacttt tgtagttcct aaaacttcat tgagtgtctg taaatatata
     1561 tagttatcaa aatcataata gtaagtactg atttgataat caagtaaatc atccttatag
     1621 gccagactga tttctaaatt attggatttc tcttttctta gaaagcggtt acctatttca
     1681 aaagaattag tagcgagatg cataccatga gtatacattt cttgtgcatt aggtaatctt
     1741 tcttgatgtg tacctgttaa tgataagata taatttggag caaatctcca gtggactgta
     1801 cctgcataag aaaaagctgt ttctttgtta ggttgaacag cttctaaaat agattttaaa
     1861 ttttgagccc tgattttatt atttttttct acataaggac tgttatattt attaggccaa
     1921 ggtttcattg aatctttaat tttttctata tcataatcca tactaacttt ctgcttttct
     1981 atacgagcac ctagttcgaa agtaaagtcg ttccagttat atttttctag gccaaataca
     2041 ctaaagtttt tagtgacatt tcgatttaat agctgctgat tatctagata ggtggtatgg
     2101 ccttcttgag aatggactgg tgataaagca ctattatcct gttccaaata ttgtagcccc
     2161 aagataccgg ttaattcccc taatggctga tgtgataact ctatgcggcc tacttttcct
     2221 gtatttttaa aaaagttagt aatcttatct ccctcgagtt cattgtgaaa ataatctata
     2281 taactcaatg aggtacgaat tttgtcaata ccggtaaatg gttgagtgaa ttctccacgc
     2341 acatcataac gtcgagtatt taaatcaata taaggagcat tatgagagaa attagtttca
     2401 tgaatatgat tatgctgatg acaatcagga cgtggattta tgtaattaat atcttgctca
     2461 tccatcaatt gaggataata taaaagataa ttttttaatc cactaattcg tgaatcgata
     2521 ccgattgcag aggcaccaca accttcatat aaatggctat gggctggtaa accatattca
     2581 tcatgacgat gtgtataaga aactcctaaa taagtattat cgtctatcca ggataatcca
     2641 agcgtaccta ctctcgactc tgcccagctt tctggtaaat attttaaatt tttataaata
     2701 ctgttttggt tttcaagatc ttctaaagaa ttataagagc tagactggta atgaggagtt
     2761 ttatagttac cagcattacg atataaccct tctgcccgta aagctacatg tgggcttaag
     2821 ccaactgtta cacctgctgt ggtcaacttc tcattactgc cagtattaaa acgtaatgta
     2881 gtattaccct ctaatccgtc ttgaggcatt tgggttggaa ttttatagtc aatgacattg
     2941 actacccctg ccgcactatt tgaactatac aaaagggtac ttgcaccacg tataatttca
     3001 atagacttag ctaaacttgg ttcaactgtg acagcgtgat ctggggacat attagacata
     3061 tccaacacgt cagcattatt ctgtaaaact ttaatacgtt ttccttcctg cccacgaata
     3121 ataggagtag atgctccacc tccatattga ttagaatgaa ttcctaattc cgtacccaag
     3181 gcatccccta gagttgtcga acgctgttta agtttactag tatcaataaa ctgattacta
     3241 acatttagac ttttgcctaa cagctcagac cctaactcat cagattgtgc agttaatact
     3301 attgtattta aggtagtaac aggcccttca gctgccaata caattttcga ggcccctaaa
     3361 gtaattaaag ctaaaaatag tttactttta tttagcataa aacaaccaaa tttaatgtta
     3421 tattataata taacacttaa aatttataaa atctactatt taaatttttg aatggtaatc
     3481 ccccctaaaa atgaagtcaa aaatgggagc ctaagctccc attcttattg atgatttttt
     3541 aattctttta gcatagcatc tcgtaaaatt tcattcatac gtgtttgata acctttgcct
     3601 tgtgctttga accaagcaag tacatcagca tctaaccgaa ttgaagtttg ttgctttact
     3661 gggcggtaaa attgattttg gcgtacagca ttgctccagt ctgtaatttc aggaatatct
     3721 gatagatcta gctgatcatc aggaaccgtg cctttagcaa gcaagcgttg aatttcagca
     3781 tcttgcttct caccaaattt ctcatttagc tctttgcgtg agtatctaac catgctcata
     3841 tttgtttcgc tccgctttag tgacttgcct tgcactaatg attcggatga tttcacagtc
     3901 atcttcatca aaaatggtgt gagccaccaa taacattagt acgcctttaa ctcgtccaat
     3961 ggtttgccaa cgttcttcac cattggtatg tctgtcttgg attgagatcc gtaatggatc
     4021 ttcaaaaaca aggcttgcag tttcgaaaga gatatcatgc tttttctgat tctttcgatt
     4081 cttagcctca tcccattcaa aatactgttc cataaaaaac attcaaattt gtatatacaa
     4141 taatgtatca cgtaattttt agtcaaaatt attggcaaga agtatcaaaa ctgctcataa
     4201 aaaagccgac ttgtttcaaa gtcggcatta agtagttgat atttaaatta atatacaaat
     4261 gtacttcgta taatcgccat tatgttaaat taaaagagtt ttaacttttt taataaaaat
     4321 attatactct aagccccaaa atttccccta acatgaattt gtaatgaaaa aatttatact
     4381 tcctatattc agcatttcta ttctagtttc tctcagtgca tgttcatcta ttaaaactaa
     4441 atctgaagat aattttcata tttcttctca gcaacatgaa aaagctatta aaagctattt
     4501 tgatgaagct caaacacagg gtgtaattat tattaaagag ggtaaaaatc ttagcaccta
     4561 tggtaatgct cttgcacgag caaataaaga atatgtccct gcatcaacat ttaagatgct
     4621 aaatgcttta atcgggctag aaaatcataa agcaacaaca aatgagattt tcaaatggga
     4681 tggtaaaaaa agaacttatc ctatgtggga gaaagatatg actttaggtg aggcaatggc
     4741 attgtcagca gttccagtat atcaagagct tgcaagacgg actggcctag agctaatgca
     4801 gaaagaagta aagcgggtta attttggaaa tacaaatatt ggaacacagg tcgataattt
     4861 ttggttagtt ggccccctta aaattacacc agtacaagaa gttaattttg ccgatgacct
     4921 tgcacataac cgattacctt ttaaattaga aactcaagaa gaagttaaaa aaatgcttct
     4981 aattaaagaa gtaaatggta gtaagattta tgcaaaaagt ggatggggaa tgggtgttac
     5041 tccacaggta ggttggttga ctggttgggt ggagcaagct aatggaaaaa aaatcccctt
     5101 ttcgctcaac ttagaaatga aagaaggaat gtctggttct attcgtaatg aaattactta
     5161 taagtcgcta gaaaatcttg gaatcattta ataatttcta aaattaacat aatacacgtt
     5221 atacgaaatg ctgtataatt atccttataa ttcaacgctg tagtttctag tttatagccc
     5281 gagcatccct gttcgggctt tttttatgac ttttcacgtt ccacgctatt atttcatcaa
     5341 tgttccacac ttttgattag ctcaattgaa taatcaggat gcctggtctt tttctttttg
     5401 gcattcatcc agctctttct tcagcgcctt tttatcctgg tacgatgcaa tcagcatgat
     5461 cgggatcagc agcggagcta caataattcc cagggcaata tctttcagtt tcggcatgat
     5521 cccctccctt agtcattatc aaaaacagca ttcagcacac ggccaaataa gccacgtttt
     5581 ttgtgttgtg gcactgccac attttgcaac gtggcatgct gtagcgtttg ggtgtggtat
     5641 gccgtatttt cttgctgtgg cattgccaca ttttctgcaa tatttaattc atgattcttt
     5701 ttattatcat tgtcttgttg tggtgtcgca tgctgttgca tgtcagtgtc gcatgccgta
     5761 gcatcttgtt gtggtgatgc tgtggcaggt gcaacacttt tcagctcaat gagtgtttgc
     5821 atacggtcaa tttgttgcat taaccgttct tcacgttctt ggtatttttt gacttcattt
     5881 tctaaatggg caataagttt ttttgtagat tcatcacctt cttgcttaga tgattcaatt
     5941 ttttggggtg tttgctcagg ttctccaaag catcggatga cctctgctag atcgaattgt
     6001 ccatcactgg agcgtgataa acgtccatta cgtacagctc tgtaaatggt tgatctatcc
     6061 acatggaatt ttttagatac ttcacttagt gataacttag ccatttacgg tgtccttttt
     6121 atttgatggc atatatccca ctttttttag gtatgggtat agttctttta ttcggtcagg
     6181 gtcttgcagc atctcagcaa tacgaatagc aaattgcgga tagctttcag ttccctggga
     6241 ataacgaccc atttcaggaa gttcagaaag tttatttgca aacagatggc gttgtgcatc
     6301 agtcatcttt gtaaagagat ctaacgtatt cggatctctt tctatcggtg ggttgtcctt
     6361 ctttttctgt ttaaaagtaa aagagaatcc tgaaattgat cgacctcttt tgtgttgctc
     6421 atacttcaca gtgatatctg tatgttcgtt aatttgttta atagcaagct caagtacact
     6481 agttttaaag cgttccatac gcttgtactc tgtatcgagt acgccaattc tttgacggaa
     6541 atcacttacc tctataatag gagtagagcc agtacttctc caagcaatta ataattcata
     6601 taagcgaaca gcataagcac tgctgagatt actaacttgc tgcaattcgt atttagtgaa
     6661 atgctcttct aagcgtgtaa ttaagggaac tatggctggg gcaaagataa gtttaaccac
     6721 tgcttcatta tctacatatc caatttcact aacccaacgg gatctatagt tctcaatatt
     6781 ccctcgttca tttatttttt gatagctaaa ttgtcttgca aataaatcat tacaggcatc
     6841 ttttaatgct tgataagccg tattgcgatg tacgccaaat tgattgatat agccttctgc
     6901 atgtacttca aggggatcat tagcattaat acctttcccg ctttctcttg cctcaacaat
     6961 ggccaataaa attaaacgtt gttctactaa atctaagtta tagcttgcat taattaaggc
     7021 attgtctttt acaactaatt ctctcatgaa gcaccagtat tatttattat gctatgacac
     7081 aataattaat tttagtgcta taagcaaggt aaacggtgtc atattgcaag gtaaacggtg
     7141 tcatattgca aggtaaacgg tgtcatattg caaggtaaat ggtgtcatat agcctttgaa
     7201 agtgttgtaa attaaggctt tcagcatgtc taaaagcatt taaaagatta aaaataatta
     7261 aaagcccagg gaatgaaaaa ttgcccttgg ttttcgcttc gctcaaactc tattgaactt
     7321 cgctttcgct cagttcgttg gggcaatttt ttggttaata cttgcgtgac tttaagaaaa
     7381 agcaaaagca actcggaatt cgcttcgctc ataaaacttt tttactcgct acgctcgatc
     7441 caggagcaaa tttctgtatg aaacttacgc tttcttaggc taaagaggac aaaaaaatgt
     7501 ttttcagagg gtctaggcgc aaattttgat agttcgctcg tagacactcg ctctatgcat
     7561 ctatctaaag ttaaaatctc tctttaaagt aaaggctctt aaatcgcatt tgaacgcttt
     7621 ctgtgaagct tttcataatt aaagttttgg gttgaaattt ttaacctgga tgaatggaca
     7681 aaaaattgat tttttacgtt tttctaaaca aggaaataaa tacttttttc catgcaaaac
     7741 caagcaccgg ccctacgtca ctttagccaa ttttccaatc caagatccgg cacatgccgg
     7801 gatttttttg tcacacacca cgcatctaac gatgaaaccc ctcaaagcct tacaggataa
     7861 ggatttcagc gattttttag ggtctcagaa acgtgtacag aaaaatggtc tttttttaga
     7921 ataagtagtt gaattatatg ttttttatgc tgtgcacatg cttgtacatg aaaaccatac
     7981 tttttttaga taaagtattg ataaatttaa taaaagtcat gtactcatag ttgtacataa
     8041 aaaaagcatt atatttaaat aagtttttgt tttagaatag attaattttg tacaaataga
     8101 tttgtacgta atttacttat tattttaatt taaattattg aattatcttg atttaatctt
     8161 gtacaaactc taatttttat aagagatgta atttatgggc gaaaaagtca aaacttatgc
     8221 agtcagacta gatccacaag tagcagaatt ctacgatcaa ttggctaatt cagaaggaat
     8281 cagaaccagt aaattgttca gtaaaatttt gactaatgat tttcaatcta ttgctgttaa
     8341 gcagcaggca gatcgtattg aagatctggt gaatcgatta gaaaagcgaa tagatgattt
     8401 tgaggaagat aataattttt ctgaaaaata ctatgaagat ttttctggac tctatttcat
     8461 gatgttatgg acattaatga aaaatggggc ttctaaagat gaggttcgaa tgatgcaagc
     8521 taaaggttat tcatatgcag atgataacta taagagggta aagtagtgtc agataatatt
     8581 attcagcttt caaagccatg tacgctttgt gaaaatagac aaaatgtaca actttttgca
     8641 ggattgatgc tgtgtggaag ttgtcaggaa aatatcaggc ttacgaatcc aagtatgttc
     8701 tcggccaatg atgagattga acaaaaagcc caggattaac ctgggcttcg cataagagat
     8761 tttatgttaa atgccgaact gaattaaaaa tagaaatgta gttgagctga tgcttgctca
     8821 tttctttatt tttagtaatt
//
