LOCUS       NZ_KF831357             2472 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Staphylococcus aureus strain A960649 plasmid pBU108c, complete
            sequence.
ACCESSION   NZ_KF831357
VERSION     NZ_KF831357.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226029
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Utter,B., Deutsch,D.R., Schuch,R., Winer,B.Y., Verratti,K.,
            Bishop-Lilly,K., Sozhamannan,S. and Fischetti,V.A.
  TITLE     Beyond the chromosome: the prevalence of unique extra-chromosomal
            bacteriophages with integrated virulence genes in pathogenic
            Staphylococcus aureus
  JOURNAL   PLoS One 9 (6), e100502 (2014)
   PUBMED   24963913
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Utter,B.D., Schuch,R., Winer,B.Y., Verratti,K., Bishop-Lilly,K.,
            Sozhamannan,S. and Fischetti,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2013) Laboratory of Bacterial Pathogenesis and
            Immunology, Rockefeller University, 1230 York Ave, New York, NY
            10065, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: Roche gsAssembler v. September 2012
            Assembly Name                :: pBU108c
            Sequencing Technology        :: 454
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009962225.1-RS_2024_05_15
            Annotation Date              :: 05/15/2024 01:31:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KF831357.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="A960649"
                     /isolation_source="NARSA"
                     /db_xref="taxon:1280"
                     /plasmid="pBU108c"
     gene            complement(495..971)
                     /locus_tag="HTC18_RS00005"
     CDS             complement(495..971)
                     /locus_tag="HTC18_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(1418..2152)
                     /gene="erm(C)"
                     /locus_tag="HTC18_RS00010"
     ARG             complement(1422..2152)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2213..2272)
                     /gene="ermCL"
                     /locus_tag="HTC18_RS00015"
     CDS             complement(2213..2272)
                     /gene="ermCL"
                     /locus_tag="HTC18_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_172685716.1"
                     /translation="MGIFSIFVISTVNYQPNKK"
                     /name="ermCL"
     ARG             complement(1419..2152)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(486..971)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taacgactta attacgaagt aaataagtct agtgtgttag actttaatgt ttttttaagg
       61 cattagtgca tttaagcgtc agagcatggc tttatgccga gaaaactatt ggttggaatg
      121 gcgtgtgtgt tagccaaagc tttggcgagt tggttggggg tttcatggga ttaatcccat
      181 gaaagtacca actcaacaac acactaacgc ctgttggttc caaccaatag gaaattggaa
      241 taagcaatta gtataatgag agtataatgt tggtataacg ttagtataat gatgcttttt
      301 ttcattatat tttttatgta ctttaaacct gcacgcttat gtgaattaga aaaagcttaa
      361 tcgcatttca tagattgacc tcccaataac tacgtggtgt tattgggagg tcaatctatt
      421 tcatttgcgt taagcttttg atctacatta agcgtgcagg ttttttgtct atttgtagac
      481 gattttatgt tatactagtc cttgaaagaa taataatcag ataatgcatt ttcttgtttt
      541 tcatttgcct cttgctcaaa gttcccaaat tcgagtaaga ggtatttttg tttttggtcg
      601 tcgcctctca ttagtagttc agggtttaac attaatactc cagtttttct ttttataata
      661 tttccttctt ctaagatttt aagtgttgtt attactgttt gtagacttgt tcctgtagct
      721 tttgctattt ctcttgttgt agctatcatt gtattgttac ttaagtggac attatctagg
      781 atatagttaa cgattttaag tttttttccg ccaatcatat ctaacatact tattaattgc
      841 actatatatg cctttacgaa gttaccagac gtttgtttac ggtataactt gtctacctct
      901 atgacttctc cactttcttc gtctatgagc ctctgagagc ctttatagac tgttccatat
      961 ctttctttca tctttttctc actccttatt ttaaactatt ctaactatat cataactgtt
     1021 ctaaaaaaaa agaacatttg ttaaaagaaa ttagaacaaa atgagtgaaa aattagaaca
     1081 aacaaattcc ttataaacct tatcatctca acctatatta agattttacc tagttgaatc
     1141 ttcttttcta tataaagcgt cggagcatat cagggggtta tctaacgtaa atgctaccct
     1201 tcggctcgct ttcgctcggc attgacgtca gatactgcac cccctgaacc cccatgctcc
     1261 aacagcaaaa aggaaacttt ttgctgcttt tccgacgctt attcgcttcg ctcatattta
     1321 tatagaaaag aagtgaatgc gcaaaagaca taatcgattc acaaaaaata ggtacacgaa
     1381 aaacaagtta agggatgcag tttatgcatc ccttaactta cttattaaat aatttatagc
     1441 tattgaaaag agataagaat tgttcaaagc taatattgtt taaatcgtca attcctgcat
     1501 gttttaagga attgttaaat tgattttttg taaatatttt cttgtattct ttgttaaccc
     1561 atttcataac gaaataatta tacttctgtt tatctttgtg tgatattctt gatttttttc
     1621 tatttaatct gataagtgag ctattcactt taggtttagg atgaaaatat tctcttggaa
     1681 ccatacttaa tatagaaata tcaacttctg ccattaaaaa taatgccaat gagcgttttg
     1741 tatttaataa tcttttagca aacccgtatt ccacgattaa ataaatctca tcagctatac
     1801 tatcaaaaac aattttgcgt attatatccg tacttatgtt ataaggtata ttaccaaata
     1861 ttttatagga ttggttttta ggaaatttaa actgcaatat atccttgttt aaaacttgga
     1921 aattatcgtg atcaacaagt ttattttctg tagttttgca taatttatgg tctatttcaa
     1981 tggcagttac gaaattacac ctctgtacta attcaagggt aaaatgccct tttcctgagc
     2041 cgatttcaaa gatattatca tgttcattta atcttatatt tgtcattatt ttatctatat
     2101 tatgttttga agtaataaag ttttgactgt gttttatatt tttctcgttc attataaccc
     2161 tctttaattt ggttataatg aattttgctt attaacgatt cattataacc acttattttt
     2221 tgtttggttg ataattaact gtgctgatta caaaaatact aaaaatgccc atattttttc
     2281 ctccttataa aattagtata attatagcac gagctctgat aaatatgaac atgatgagtg
     2341 atcgttaaat ttatactgca atctgatgtg attattgaat aaaagatatg agagatttat
     2401 ctagtttctt tttttacaag aaaaaagaaa gttcttaaag gttttatact tttggtcgta
     2461 gagcacacgg tt
//
