LOCUS       NZ_HG941720             4080 bp    DNA     circular CON 07-MAY-2024
DEFINITION  Escherichia coli O25b:H4-ST131 strain EC958 plasmid pEC958B,
            complete sequence.
ACCESSION   NZ_HG941720
VERSION     NZ_HG941720.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA2272019
            Assembly: GCF_000285655.3
KEYWORDS    RefSeq; complete genome; complete replicon.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Forde,B.M., Ben Zakour,N.L., Stanton-Cook,M., Phan,M.D., Totsika,M.,
            Peters,K.M., Chan,K.G., Schembri,M.A., Upton,M. and Beatson,S.A.
  TITLE     The complete genome sequence of Escherichia coli EC958: a high
            quality reference sequence for the globally disseminated multidrug
            resistant E. coli O25b:H4-ST131 clone
  JOURNAL   PLoS One 9 (8), e104400 (2014)
   PUBMED   25126841
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Beatson,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2011) The University of Queensland, Centre for
            Infectious Disease Research, St. Lucia, Brisbane, QLD 4072,
            AUSTRALIA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000285655.3-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 02:03:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,181
            CDSs (total)                      :: 5,066
            Genes (coding)                    :: 4,850
            CDSs (with protein)               :: 4,850
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 79 of 216
            Pseudo Genes (incomplete)         :: 152 of 216
            Pseudo Genes (internal stop)      :: 39 of 216
            Pseudo Genes (multiple problems)  :: 48 of 216
            Pseudo Genes (short protein)      :: 3 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HG941720.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /strain="EC958"
                     /serotype="O25b:H4"
                     /db_xref="taxon:941322"
                     /plasmid="pEC958B"
     gene            join(3926..4080,1..92)
                     /gene="mobC"
                     /locus_tag="EC958_RS26370"
                     /pseudo=""
     CDS             join(3926..4080,1..92)
                     /gene="mobC"
                     /locus_tag="EC958_RS26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /name="mobC"
     gene            625..1332
                     /locus_tag="EC958_RS26380"
                     /old_locus_tag="EC958_B0001"
     CDS             625..1332
                     /locus_tag="EC958_RS26380"
                     /old_locus_tag="EC958_B0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1588..3747)
                     /gene="mobQ"
                     /locus_tag="EC958_RS26385"
                     /old_locus_tag="EC958_B0002"
     CDS             complement(1588..3747)
                     /gene="mobQ"
                     /locus_tag="EC958_RS26385"
                     /old_locus_tag="EC958_B0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029344.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALVNAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(3390..3748)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3749..3925
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          626..1331
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1592..3834)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.733"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccagttcct gaaggcagag gtgacacgac aggctgatgt gacgcgcata ctgccgctga
       61 ttaacgagct ggaagcgctg ccaggacagt gattgtatcg tccgctcgcc gcagtcccag
      121 cgaccgagcg ttagcgagcg agtgggcgag gaagcggaag tgaatccgtc gcctgattga
      181 gcacatacgc acggtttttc gggaggtgat atgcgctgaa cccgcatgaa tactggattt
      241 ccacagggtt ggagggggtg tattcggaac ttgcaattct gctttttttg caacgcattg
      301 tttttattgg ttttttgttt tcagcactcc aagttatcca caggctcgtc gttaagtttt
      361 tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agcgccaaaa ttccttatag
      421 aaaacaaaat gttacaatgc tataggtaat atgatttaag gtaaaggtga tataatttaa
      481 ggtgcttggg tgaggtaatt taaggtaaag gtgaggagat ttaaggtaat attcacagga
      541 aaatgggggg tgaaaatata attgctcacc aatgctaaag gtgataggtt tttatttcac
      601 ctcatttttg gcctggtggt taaaatgaca tcaaaccctc tgatagccta taagtccaat
      661 gcgctggttg aggccagtta caagctaact ttacaagaac agcgtttctt attgctctgc
      721 ataagccgac ttaactccgg tactgacgta gcttcacctg aattgcaaaa gacaatgacc
      781 attacagctg cggaatattt tgattcattc cctgatatgg ggcggaaaaa tgccgaggtt
      841 cagctacagg aagcgatcga tcgtttgtgg gacaggtcga tcattctcaa ggacgacgag
      901 aagcgcgagg agttccgctg gattcagtat cgggcacagt acgcgagggg agaggggaaa
      961 gcacaaatca ctttttctga cgcggtaatg ccttatctga cacagttgaa ggggcaattc
     1021 accagagtcg tcattaaaaa tatttctaat ttatcaagaa gttacagcat aagaatatac
     1081 gaaattctcc agcaattccg cagtactggg gaacgtatta tcgccctcga tgattttaaa
     1141 tcatcactga tgcttgatgg taaatataaa gatttcaaaa cgttaaacag ggatctaata
     1201 aaaccctgtg ttgacgaact gaacaaaaaa agcgacctgg ctgtaacggt tgagacgatc
     1261 aaaaaggggc gcacggttgt agccctgcat tttcgcttca aggaagacaa acaaatcaag
     1321 atgacgatct aaaatcgatt ttaaggcacc taaaacgctc cctgctgcgt tttttttgtg
     1381 ttagtagtgc gaaggtacta ttgaagtgaa aaattgcgtc aaaagcgctc taatgcagcg
     1441 aaaaaagaac ccccattttt tgggggcgga aaaacaaaac tatgcttgca ttatgtgtgt
     1501 gcttcttgca ttcatccctg tcgatgaaca tcgaagaaca tcgacgaaca taacacgatg
     1561 ttaaacaaaa caaaatattg ttcacattca ctgtgaccaa ccgcgatcat gtctcctctc
     1621 ggtggcgtgt gttcgtgcat cttcctcgcg ccgccattcg tcccaggtac gattaaaacc
     1681 cagcgagcgg aacagatgat ccggctgttt ttctggtgtg ttgacggcag caaaaagcgg
     1741 tgcctcacgg gttttcagat gaaactccat aagttccggc gcaggtgata tgtggctgtc
     1801 tgcatcgtga tttacggcaa caccataccc tgcataccgt aaccccttat cggtcagtgc
     1861 actgatgacc agctggctgt tatcggctga gggaacggca aacgcgggat acagcgtacc
     1921 attaagccgg acaacaccgc ccagagagtc atccacgccc atgaccagca aaagctgaag
     1981 ctggcgggtt tcccggtcga cagaactcat taccggcatc agcgtcggta gcagacgtcc
     2041 ctgcgtaaaa tccaccttcc ttgctgccag tgatggcatg gctggctggc ggtcttctcc
     2101 aagacagaaa gactccggcc atgtcttcgg ctggcggaga ttgcgggtat attgcggcca
     2161 tgtctggcaa acctgcgtca gaagttgctc aaaggtcgca gaggaataaa cacgttgcag
     2221 tggtggatgt tgaccgattt cgcttcggtc acagatataa ccactcctgt cacgcattaa
     2281 atcaacacgc tcaaagattc tggtgcggta tttctccagc tcgttccccc agagcgtgta
     2341 ttctccgttc cggttgtgaa gcagaaggta acaggtcatt tccccgttac caggcatggg
     2401 ggcctcgcca taccccgtaa cgactgcgct cagtacccct gaatccataa cggcattgtc
     2461 atttttcctg agctgttccg ctcgtcgctc cgcgcgacta cgttctgtat cctcctgctc
     2521 cttccggcgc aattcctctt cacgtttaag caactggact ctgagagctg caaggccgga
     2581 tgccccccac tgctgccact gtttttcaaa tgccgtctga ctcgcgggaa tgtcctgttt
     2641 ctccaccttc cggtaatgct gtcgcaattt ttccatttgc tggctcagcg actggatttt
     2701 tccctgttcg ctacggtatt ccggtgccgc ccattgcatc aggggaatac gggaaaaacc
     2761 ggacgaaaac ggcgatgatt ttttcaggct ggcagcatgg ctcatcgctt cctttcgttc
     2821 ggtgttcagc tcttcccagc gtctgcgcgt cgcctgcata tctgccatca tcgacatggc
     2881 attactggtg agtttccatg ccgcattact gtccttcggc aacgcttcga tgatgtcacg
     2941 tagtgtgtac gaagttgcgt tgtcaggaag cacgatattc agaaggtcag ccacacggcg
     3001 ggcaggctct gtcagcccgc tgtttctgag ttcggtacgg gaaatctgta tttccaggtt
     3061 aagtctgtta atcagacgat tctgttcgcc gcgttctgtt tcgataccgc gtgtttccat
     3121 cgcacaggca gactttccga ggtgaatacc aggcgttttc tcaagcccct gtcgctcgta
     3181 tgaacgatgg tctatctctt cctgataacc ggcgttcacc agtgccctgt tcgcatggtc
     3241 agcccatgat gcacgccacg tctcggcatg tgtccggtcg ttccagtccc tgacctttcc
     3301 accgaatcca gcaggtccga cagctctcgt ggtcagcatg atgtgcgcat ggggatttgt
     3361 tttatccatg tgatggaacg ccacatcagc aatcatgccc tgactgacaa agttttctct
     3421 gacaaaggcg agaacggttt cccgtgcggc atcctgcggt agttcgcggg ggatggcgag
     3481 ttcaatttcc cgcgcaagtt gtgagttctt ccggcgttcg gccttctcaa cggcattcca
     3541 gagttcggca cggttcgtac accaggcagg ggctgtcgcg ggagtcagaa tcacagcttc
     3601 agccacaccg cttttgcggg tgtagtcgtg agtcagtccg gtacgctcat caagcatcag
     3661 tgaaccggaa cgataggcag cagaagcaac ggcgctgtag ccgctgtttc tggagatgat
     3721 tttcatgctg agatgataaa tcgccatttc tgccgtaatg cctttttgtg gttttctttc
     3781 agccccgcag ggcgcacaca atcgaaacgg agtggagatt gtataagtgc gcacttcgtg
     3841 attttccgtg cattatgcca gctcagtttt taaactacca gtgacgcgct aaaatgcgat
     3901 ttttcatcgc gcacggagtt acatcatgag cagaacactc gaacagaaga ttgctgatgc
     3961 tgaggccaga ttacagcgcc tgaaggcgaa gagcaggagt ctggacacag cgcagaaggt
     4021 cattgtgggt gcagcattgc tggcaaaggt cagaaagccg gaggaggtgc agttgcgtgc
//
