LOCUS       NZ_HG813244            11152 bp    DNA     circular CON 06-APR-2024
DEFINITION  Staphylococcus epidermidis PM221 plasmid 3, complete sequence.
ACCESSION   NZ_HG813244
VERSION     NZ_HG813244.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA3139103
            Assembly: GCF_000751035.1
KEYWORDS    RefSeq; complete genome; complete replicon.
SOURCE      Staphylococcus epidermidis PM221
  ORGANISM  Staphylococcus epidermidis PM221
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Savijoki,K., Iivanainen,A., Siljamaki,P., Laine,P.K., Paulin,L.,
            Karonen,T., Pyorala,S., Kankainen,M., Nyman,T.A., Salomaki,T.,
            Koskinen,P., Holm,L., Simojoki,H., Taponen,S., Sukura,A.,
            Kalkkinen,N., Auvinen,P. and Varmanen,P.
  TITLE     Genomics and proteomics provide new insight into the commensal and
            pathogenic lifestyles of bovine and human-associated Staphylococcus
            epidermidis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   Kammonen,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2014) Institute of Biotechnology, University of
            Helsinki, Viikinkaari 4, 00014, FINLAND
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000751035.1-RS_2024_04_06
            Annotation Date                   :: 04/06/2024 01:19:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,528
            CDSs (total)                      :: 2,444
            Genes (coding)                    :: 2,349
            CDSs (with protein)               :: 2,349
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 67 of 95
            Pseudo Genes (incomplete)         :: 42 of 95
            Pseudo Genes (internal stop)      :: 28 of 95
            Pseudo Genes (multiple problems)  :: 34 of 95
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HG813244.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Staphylococcus epidermidis PM221"
                     /mol_type="genomic DNA"
                     /strain="PM221"
                     /db_xref="taxon:1449752"
                     /plasmid="3"
     gene            1..867
                     /locus_tag="SEB_RS11850"
                     /old_locus_tag="SEB_p302423"
     CDS             1..867
                     /locus_tag="SEB_RS11850"
                     /old_locus_tag="SEB_p302423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494364.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_018113587.1"
                     /translation="MTGETVVYKNDLNLVPLRKFTSTEINLFFAMCNKLKEKETNTLHL
                     SFDELKELSNYSPKTRNINRFVKDLDDVYKKMLELTIRYEDDEVIERFILFNHYRIHKK
                     DQYLEISTSPNLKHILNSITSNFTKFELKEMTNLKSTYSKNMFRILKQYKHTGYMKIKI
                     DDFRERLDIPKSYRMTDINKNVLKPIVTELNAIFNNLHINKIKAKKGRKIEWLEFIFDP
                     EKRIHNKRQPQMTNVGKQRQYINREKTPEWLNEGRLGQSQEIHNEDSKLRQDREAFQRQ
                     LEEKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            1535..2101
                     /gene="lepB"
                     /locus_tag="SEB_RS11855"
                     /old_locus_tag="SEB_p302424"
     CDS             1535..2101
                     /gene="lepB"
                     /locus_tag="SEB_RS11855"
                     /old_locus_tag="SEB_p302424"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503824.1"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA];
                     GO:0006465 - signal peptide processing [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="WP_018113588.1"
                     /translation="MKKEFKEWLIAIIVAILLLFLVNNFLFKPYTVSGLSMYPTFNNKD
                     KVVVSKISKSLNHLKSGDVIIFHQNSNNDFIKRLIGKPGDQIEYKNDKLYINKNYIKEP
                     YLDYNKKMNNSSENLTEDFNVSNIKGSKHKMIIPKDKYLVLGDNRTNSIDSRYSEVGLI
                     SKKQIVGKVILRFWPFNDMKYNFKR"
                     /name="lepB"
     gene            complement(2302..2400)
                     /locus_tag="SEB_RS13425"
     CDS             complement(2302..2400)
                     /locus_tag="SEB_RS13425"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_309498286.1"
                     /translation="MMYLIFVFIIAPIIVECSVTLFKYWLEQRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2740..3105)
                     /locus_tag="SEB_RS11860"
     CDS             complement(2740..3105)
                     /locus_tag="SEB_RS11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494365.1"
                     /translation="MKWLKIIILILSLVPIEFIGLFTDYQTGLLIGYIPFIIVAILISR
                     SIFKFGLKNNISIIISRCIGTFLSWECVHWFMNIYDSSDYFKPLTTDIFALFLGAIHFI
                     VIMLIYLVIYGFSHRNN"
                     /name="hypothetical protein"
     gene            complement(3235..3603)
                     /locus_tag="SEB_RS11865"
                     /old_locus_tag="SEB_p302411"
     CDS             complement(3235..3603)
                     /locus_tag="SEB_RS11865"
                     /old_locus_tag="SEB_p302411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_002494366.1"
                     /translation="MKKILKILITSIIVIIITLTVWTFSVITYQEHKSEKIIDHVIERK
                     GWDKKIKNEKMSFNIMMGYAEKDIAFKDQPYSEYEYNVTPAPAPWTDDKEYKVWGETDL
                     QKKDSYYKYLLESEPYRK"
                     /name="DUF3139 domain-containing protein"
     gene            3707..4102
                     /locus_tag="SEB_RS11870"
                     /old_locus_tag="SEB_p302412"
     CDS             3707..4102
                     /locus_tag="SEB_RS11870"
                     /old_locus_tag="SEB_p302412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_002494367.1"
                     /translation="MKRKYKITGIIFIVLLIVLTLIFSIVHHYANVQKHEEAKLKQKVH
                     HIFKQKGWEDKVKEEKNIFTFNTGDNDLQVTFKDEPYNTYTYSIDEDNKVYGNAVLKDE
                     YDKDFDSKKKYKEYLRKMHFEEKYDLK"
                     /name="DUF3139 domain-containing protein"
     gene            4336..4515
                     /locus_tag="SEB_RS13950"
                     /old_locus_tag="SEB_p302413"
     CDS             4336..4515
                     /locus_tag="SEB_RS13950"
                     /old_locus_tag="SEB_p302413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001829546.1"
                     /translation="MKSIVHFICLFIIFCIFGYVIDLIFFKEINIFLILESAFILSLLI
                     MPILKIVKEIEIEK"
                     /name="hypothetical protein"
     gene            5023..5163
                     /locus_tag="SEB_RS13820"
                     /old_locus_tag="SEB_p302414"
     CDS             5023..5163
                     /locus_tag="SEB_RS13820"
                     /old_locus_tag="SEB_p302414"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_235203037.1"
                     /translation="MKNELKGSYFYTAAKKGTGVTISYDMYTATTGPNHTIAKNFKHSY
                     N"
                     /name="hypothetical protein"
     gene            complement(5323..5709)
                     /locus_tag="SEB_RS11880"
                     /old_locus_tag="SEB_p302416"
     CDS             complement(5323..5709)
                     /locus_tag="SEB_RS11880"
                     /old_locus_tag="SEB_p302416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494369.1"
                     /translation="MKKAIFSIIISLILVLTVTGCSNSSKEKPIKKSALEINPTSKAVN
                     ITINKKENNKPEKIGKVYRYKNNNAKEITNDGIKKDTKDTLIWKGVANKYDNVKDLLGE
                     SILYEVKYKNGDIKKFERKIKYTE"
                     /name="hypothetical protein"
     gene            complement(5729..5890)
                     /locus_tag="SEB_RS13595"
                     /old_locus_tag="SEB_p302417"
     CDS             complement(5729..5890)
                     /locus_tag="SEB_RS13595"
                     /old_locus_tag="SEB_p302417"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162153024.1"
                     /translation="MNEREQRQKLNITLPPKLITNLDKVKTKYGINKSKQIENLISKYL
                     NEEYGNLE"
                     /name="hypothetical protein"
     gene            6651..7025
                     /locus_tag="SEB_RS11885"
                     /old_locus_tag="SEB_p302418"
     CDS             6651..7025
                     /locus_tag="SEB_RS11885"
                     /old_locus_tag="SEB_p302418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494371.1"
                     /translation="MKKIVYILGTIIVLGFCYFYVNLIFFDSWTRYSAEKEINNYISNH
                     DTKKLKEISANNKTYQLLKHAKHVTIKGDTDNQGGGHIGYFTSTINGKEGALFVHLNFG
                     LFPEIPKVTKLEVFDKNIYE"
                     /name="hypothetical protein"
     gene            complement(7616..8068)
                     /locus_tag="SEB_RS11895"
                     /old_locus_tag="SEB_p302420"
     CDS             complement(7616..8068)
                     /locus_tag="SEB_RS11895"
                     /old_locus_tag="SEB_p302420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494373.1"
                     /translation="MPKKQSSKEKILTVRVSQAQYKKIQDLAYIRSMNVTEYIRQTAVG
                     NRIKPTVIEYPKEQITMDDSNSKNNTNNLYKVIEHLEEDNEALKSDRDALRQRHDQLED
                     DIEQYKRENEIFHRLLEHFDSTAFMNFNTYRDDRPLKKEIKRLKEH"
                     /name="hypothetical protein"
     gene            8523..9731
                     /gene="mobV"
                     /locus_tag="SEB_RS11900"
                     /old_locus_tag="SEB_p302421"
     CDS             8523..9731
                     /gene="mobV"
                     /locus_tag="SEB_RS11900"
                     /old_locus_tag="SEB_p302421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_040118613.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLV
                     EDERHLYHRVKDRVDYYKEQGSRVQKNSVVMYSNIITLSEEEADRMGETRTKHYFKTCK
                     DYFSERFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     HLTRDIQNLKRKNHFLQEDSQQAQEDIDLYKQLRANSRDDFEKDEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQYLTTQNNQLEHHLDMLNARHQAMNDMVRNDTRILFAGLRCALKD
                     RNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     gene            complement(10490..10645)
                     /locus_tag="SEB_RS11905"
                     /old_locus_tag="SEB_p302422"
     CDS             complement(10490..10645)
                     /locus_tag="SEB_RS11905"
                     /old_locus_tag="SEB_p302422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="WP_018113591.1"
                     /translation="MEHISKLGEAIVNTISASQSGDSAELAKSIVNIAANGIGVISDVA
                     KAFGLM"
                     /name="beta-class phenol-soluble modulin"
     Typing          2..867
                     /name="rep_cluster_1439"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8452..9728
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgacgggag aaacagtagt atacaaaaac gatttgaatt tagttccttt aagaaaattc
       61 accagtactg aaattaattt attttttgcc atgtgtaata aacttaagga aaaagaaact
      121 aacacacttc atttatcttt tgatgaattg aaagaattga gtaactatag tccgaaaaca
      181 cgtaatatta atagattcgt taaagacttg gatgatgtat ataagaaaat gttggaacta
      241 acaattagat acgaagatga tgaggttata gaaagattta ttttatttaa tcattataga
      301 attcataaaa aagatcaata cttagaaatt tcaacttctc ctaatttaaa gcatatactt
      361 aattcaatta ctagcaactt tactaaattt gaattaaaag aaatgacaaa tttgaaatct
      421 acatattcaa aaaacatgtt tagaatactt aaacaatata aacacactgg ttatatgaaa
      481 attaaaattg atgattttag agaacgccta gatatcccta aatcttatag aatgacagat
      541 attaataaaa atgttttgaa gcctattgtt acagagttaa acgctatttt taacaatctt
      601 cacattaata aaatcaaggc taaaaaagga cgtaaaattg aatggctgga gtttatcttt
      661 gatcctgaga aacgcattca caataaacga caacctcaaa tgaccaatgt aggtaaacag
      721 cgtcaatata tcaatcgtga gaaaacacca gaatggttaa atgaaggaag attaggacaa
      781 tctcaagaaa tacataatga agattcaaaa ttaagacaag atcgagaggc atttcaacgt
      841 caattagaag aaaaatggga ggaataataa cttaaatgag ctacatctat attttgcttt
      901 tatttccttt aataatggga tttttcttat cactaaaaga gaaaaagtat gataaaaaat
      961 gactaattca ctattgtaaa gaaaatacag aattttatga gtaaaacttg taattttatt
     1021 gcaagtgaag aaactacagt atatactgaa actaataaat attgaactca tatatcaccg
     1081 aaccccaacc tactgacata ggttgggttc tttagtggta aggctatctt gacttattta
     1141 ttgcggttgt ttagccaaaa catgaaaatc gcaacaccgc atcctactat aatttgaata
     1201 aagcattaaa aatggccact caacaatttg agtggcattg aaatcgttaa ggagttactt
     1261 acgttaaaaa aattaatgtt agttagggac aattatatgt tttcacttta acacttattt
     1321 atatttaact cactcaaaaa atatttaacg ctggaaattt ttgccttttt tgaaacgagt
     1381 ggaacgagtt cattctgttc ttgatagtaa attttattat ttcaataaag aaaagacatc
     1441 ataaataatg aactactttt ggtaggatat taaaaatata agtgataacc taaaatagaa
     1501 taaagtttat aaaatctaaa aaggaaatta tgtaatgaaa aaagaattta aggaatggtt
     1561 aatagccata atagtagcta tattattatt atttttagta aataattttt tatttaaacc
     1621 ttatactgta tcaggattat caatgtatcc aacgtttaac aataaagata aagtagtagt
     1681 cagcaaaata tccaaatcat taaatcactt aaagtctgga gatgttatta tatttcatca
     1741 aaatagcaat aatgacttta taaaacgatt gattggtaaa ccaggagatc aaattgaata
     1801 taaaaatgac aagttatata tcaataaaaa ttatatcaaa gaaccttacc ttgactataa
     1861 taaaaaaatg aataactcca gtgaaaattt aacagaagat tttaatgtat caaatataaa
     1921 gggatctaaa cataaaatga taatacctaa agataagtat ttagttctcg gagacaatag
     1981 gacaaacagt attgatagta gatattctga ggtggggtta atatctaaaa aacaaatcgt
     2041 aggaaaagtt attttaagat tttggccttt taatgatatg aagtataatt ttaagcgatg
     2101 aatgataaaa aagataagtt ataaagctag cttgagatat tgttgacgat tacataatta
     2161 gttcgttcaa atatctattt aattttacaa atctatactt acaagatata ctgaaagtaa
     2221 caaatattaa gtgcatcaaa acaccaatcc cccaatctat tgcagtagat tgggggatct
     2281 tttagtgttg actatgtcgt cttatttttt attacgttgt tccagccaat atttaaacaa
     2341 cgtaacactg cactctacta taataggtgc tattataaaa acaaatatta agtacatcat
     2401 tacacctcct tccgtcgaat ctatcgccgg aagtaagacg acttcaatag tataacaaaa
     2461 atttttaatt taaaacgata gtttaggact tgccatatga ataaagatac aaaagttcac
     2521 ttgatgaaaa agaatgaaaa cgcatggtta agggaatgta taaaacctga ataaacgata
     2581 atcataagac aaaggcttaa acgctgttaa atcaacgttt aagccttttt gtgtagttaa
     2641 cataattaat tattatgggt agtgcctttg tataaatact caaaaacaca aacattgtta
     2701 aattaatctt tgtgtccttt aatataagta acataatagt taattattgc gatgagaaaa
     2761 accatagata actaaatata taagcataat aactatgaaa tgaatagctc ctaaaaataa
     2821 tgcgaaaata tctgtagtaa gaggtttaaa ataatctgat gaatcgtaaa tattcataaa
     2881 ccaatgaaca cattcccaag aaagaaatgt tccaatacat ctacttatta taatacttat
     2941 attatttttt aagccgaatt taaaaataga tctacttatt aatatagcaa ctattataaa
     3001 tgggatgtaa cctattaata aacctgtttg ataatcagta aataatccga taaattctat
     3061 tggaactaaa gataaaatta atataatgat ttttaaccat ttcattttaa aatttctctt
     3121 ttcaatatta tttttccttc aatatcattt tattaatata attaggtaaa agtctacgct
     3181 gtctttaagt gatagatgaa tgatctctat tttttattat gttatacatt tatattattt
     3241 tctgtaaggt tctgattcta aaagatattt ataataggag tctttctttt gtaaatctgt
     3301 ttccccccat accttatatt ctttatcatc tgtccatggt gctggtgctg gtgtcacgtt
     3361 atactcatac tcactatatg gttgatcttt aaaagcaata tctttttcag catatcccat
     3421 cataatatta aaactcattt tttcattttt tattttttta tcccaaccct tacgttctat
     3481 aacgtgatcg atgattttct cactcttgtg ttcctgataa gtaatcacac taaaagtcca
     3541 aactgttaag gtaataatga taacaataat agaagttatt aatattttta agattttctt
     3601 catttttgta taattcctct tttttaataa tttttatgat aaatatgtat tattgtattt
     3661 aattttacaa aatattaatt aaataaattt acttaaggag actaatatga aaagaaaata
     3721 taaaattaca ggtatcatct ttatcgttct tcttatcgtt ttaacattaa tttttagtat
     3781 agtgcaccat tatgctaatg ttcaaaaaca tgaagaagct aaactaaaac aaaaagttca
     3841 ccatattttc aaacaaaaag gttgggaaga taaagttaaa gaagaaaaaa atatatttac
     3901 gttcaatact ggagataatg atttacaagt cacttttaaa gatgagcctt ataatacgta
     3961 tacatattct attgatgaag acaataaagt atatggaaat gctgttttga aagatgaata
     4021 tgataaagat tttgatagta aaaaaaagta caaagaatat ttaagaaaaa tgcattttga
     4081 agaaaaatat gatctgaaat aaaaaagcaa gcgactacga taattgatag tcgcttgctt
     4141 tttagtgttc tttcaatttg tttaatgctt ttttatgatt cgttttctat cttgtaattt
     4201 ggaggcttat atatgccatt ttaaaagcat atgcgcgtct ttaatatgaa atatactaaa
     4261 attacctata tgatattaaa tgaaaagcct gtataaagtt taaaatagct tagaaagcaa
     4321 aaaggagtat tagcattgaa atcaattgtt cattttattt gcctctttat catattttgt
     4381 atttttggat atgtgataga tttaatattt tttaaagaaa taaatatttt tctgatttta
     4441 gaatcagcct ttattttatc attactaatc atgccgatat taaagattgt aaaagagata
     4501 gaaattgaaa aatagaactg gtaaatacca ttttctatac ttaatgttta ataaaaatag
     4561 gctactaaaa ttcagttaat aaagatccat ctttattaac tgaattttaa aaaatatata
     4621 tagtgttaat ttttttaatt aaagtagtta taacttaatt tatatgataa agtcatattt
     4681 aaacatataa atattttaca ggaggtcttt ttatgaaaaa gttactttca gtttttattt
     4741 gcaagtttat tagttttaac cggattatct ttttcttcaa ttaatcaagg taatactgcg
     4801 agtgctaaaa caaaatataa aacaactata acttacaaag gtcaaaaata tgtatatgtt
     4861 ggtcattata aacaccattt ttctaaaaaa gaagttaaaa aagtagttaa attttctaaa
     4921 ggagttcatt caggaaacaa attagtatct gctgctagta aacttagtaa aaatggttat
     4981 gttaaagcat cctcggctat ttataaagcc tttgattttg gattgaaaaa tgaattaaaa
     5041 ggtagctatt tttacacagc agctaaaaaa ggtacaggcg taacaatttc ttatgatatg
     5101 tatactgcaa caacaggtcc gaaccatact atagctaaga attttaaaca tagttataat
     5161 taaataaatg tgaggggaag gattaatcct tcccctcaca tttattttca agcttttttg
     5221 aacggttgaa ttttagacca taatttatga attcatagat attagataat atttcagcta
     5281 ttattcttaa aatattttcc attatcatca ctactttcaa ttttattcag tatatttaat
     5341 ttttctctcg aattttttta tatccccatt tttatattta acttcataaa gaatactttc
     5401 tcctaataaa tcttttacat tgtcgtattt gtttgctaca cctttccaaa tcaatgtatc
     5461 tttagtatct tttttaatac cgtcattagt aatttctttt gcattgttat ttttatatcg
     5521 atacactttc ccaatttttt caggtttgtt attttctttt ttatttattg taatattaac
     5581 agctttactt gtaggattaa tttctaatgc acttttttta attggttttt ctttagaact
     5641 attactacat ccagtaacag ttagaactaa aataagagaa ataataatac taaatattgc
     5701 ttttttcatt ctaatcatcc ttttttgctt attcaagatt accatattct tcatttaaat
     5761 attttgatat taaattttca atttgtttag atttattaat accatatttt gttttaactt
     5821 tatctaaatt agttattaat ttaggaggaa gagtaatatt taacttttgt ctttgttctc
     5881 tttcattcaa acccacaaca ctctcctttt atttatgcgt ataaagtagc attacatgcg
     5941 tacaaagtca agtttgtacc cataaaagaa gtatagtata gtttgttttt tactttatgg
     6001 agctattata attacgacaa acgataaaag aaaaacaatt ttttcctctc aaaaaagccc
     6061 tatagttttc aactaagaat aaatgagcaa agcaaatggt aagatagtcc ttcagctatg
     6121 ctgaatacta ccttaggcgg taatggattt ccttatgctc tttaaaatac aaaagttaca
     6181 ttatatacct actttttcag gttttagctt ttttctaaga agaacaattg agataaaaaa
     6241 tatattttcc tttctgaaag tgttgagaaa gaaattttaa aatgatttat agcctatctg
     6301 ttgaacaaaa aagtttttag tcatttttta ataaaatgtt ctagaaaggg cttaaaatgc
     6361 aaaataaaaa gactactcaa aaaatgagta gtctgaagaa tagtttttga tgtattaaat
     6421 gaaagggagt actacattaa tcatatcaca cgcatttata cagagaaaat ttattgatgt
     6481 tgagaagaac ccttaactaa acttgaagcc gaatgtcggc atagcgtaag ctattaagcc
     6541 gacgattcga taagtttagg gattgttaaa gattcggagg ttcaacgtca ataaagcaat
     6601 ttaatttacc ataaaaactt aaatattttg aggagataag gagtctttaa atgaaaaaaa
     6661 tagtttatat acttggtact attatagttt taggcttttg ttatttttat gtaaatttaa
     6721 ttttttttga tagttggaca cgttattctg ctgaaaaaga aataaataat tatatttcaa
     6781 atcatgatac aaagaaacta aaagaaatat ccgcgaataa taaaacatat caacttttga
     6841 aacatgctaa acatgtgaca ataaaaggag atacagataa tcaaggtgga ggtcatatag
     6901 ggtattttac tagtacgatt aatggtaaag aaggagcgct ttttgtacat cttaattttg
     6961 gtttgtttcc tgaaattcct aaagtaacca agttggaagt ttttgataaa aatatttatg
     7021 agtagcataa ttgctactca tttttcgtgt tagttattct ggttctgttg caaagttaga
     7081 aaaatatagc taattacgtc catatagcat taagcttcta tgctatgata atcgttagaa
     7141 atctggctaa gaatctggag tgtttttatg atttttttaa gagaaaaaat agtcatgaaa
     7201 tatacaaaaa aatcatgtat attttgttaa atagtacagt gatagcattt atattattgc
     7261 taattgtgaa attaatattt gatttccttt tttagactat attcatattt tttcagattt
     7321 gtatttttat tggggttaat tattggtagt atcccagatt ttatagaaaa gaaaggttaa
     7381 ttttatgtta ttaaaaaata agatttattc aggcattatt attgttattt ttatagtttg
     7441 tttgattata ggattgaatg aaaatatcat tggaaatcct ttaggagaaa aaatatttta
     7501 tttattaggt tttctatttt ttgtattatt aattattggt acaacaaaaa atagaaagca
     7561 ttaatgtctt ctttttgccc catttcttac gataaacatt ttttgagtag cctttttaat
     7621 gttctttcaa tcgtttaatt tcttttttta atggtctatc atcacgatac gtattaaaat
     7681 tcataaatgc agtactatca aagtgctcta acaagcgatg aaatatctca ttctcccttt
     7741 tgtattgttc tatatcatct tcaagttggt catgacgttg tcgaagtgcg tctctgtctg
     7801 atttgagtgc ttcgttgtct tcttctagat gttcaataac tttatacaaa ttattcgtat
     7861 tattcttaga attagaatca tccatcgtta tttgctcttt tggatattca atcaccgtcg
     7921 gtttaatccg attacccaca gctgtttggc gtatatattc tgtgacattc atactacgta
     7981 tgtaggctaa atcttgtatc tttttgtatt gcgcttgact gacacgaacg gttaagattt
     8041 tttctttcga gctttgtttt tttggcattg aatcacctca taattaatat atgataagca
     8101 tacatatgtg tgcttatttt tatattaata taacatacat atgtatgtta ataaatatgt
     8161 atgattcgag gtgttgtgag ctaacatttg gcatacatct tatcaaacat atttgtttga
     8221 taatgcgtat gtcatttgtt agctatagaa cggaattcaa aaatatattt ttgaatgtag
     8281 tgatggcctc gccagagcga agataactct tacatttgaa tgtaagagta tatcgagcta
     8341 tactctcatt gcggaatcag cctcacatgt ttgaggcgta gcggtgagag tatccatcat
     8401 tttacataag cgaagcattg tcgaaggtag acaatcacag cgcttgtaaa atgatatttt
     8461 cgaaggttct cgaaagtaga gaaccacaga caaatcaaaa agcttcgggg agggcgctca
     8521 atttgaaatt agcttggaat acgcaaaaaa ataaaatgag cgatgttaaa ggcaaagaga
     8581 tggaacaaga acgtaaaggg agaatcaaaa atgatgagat tgatttgaaa cggacaaact
     8641 taaactttga tttagttgaa gatgaacgtc atttgtatca tcgtgtgaaa gatagagtcg
     8701 attattataa agaacagggg tcacgtgtac agaaaaatag tgtggtgatg tattccaata
     8761 tcattacgtt atcggaagaa gaggccgacc gtatgggaga aacacgtaca aaacactact
     8821 ttaagacatg taaagattac ttcagtgaac gttttggcga agccaatgtc gtttcagcga
     8881 aagtgcatat ggatgaatca gcgcctcata tgcaccttca ctttatacct gtgaatcatc
     8941 aagggcggtt atcagcacgt acagctatga atcgacaagc gattaatcac atccatgatg
     9001 aattaacaac acatctgtgc cagcaaggtt ttgatgttga acgtgggagt accgatgata
     9061 ataaaacgta tattcaagat attcatgaat ataaaaagaa agcgaaagtt ttaagtgaat
     9121 tagaccaaca aattgaaacg aaaaaacaat tgttgaggga tatcaacgat ttacacgatg
     9181 aaaaagaaca tttaactcgg gatattcaaa acttaaaacg gaaaaatcat tttttacaag
     9241 aggattctca acaggctcaa gaagatattg acctatataa gcaactgaga gcaaattcta
     9301 gggacgattt tgaaaaagat gaggaaaagt accaaaaaga aatcaaagcg tatgaggcgt
     9361 ctctgagtgg ccttagaatg aattaccgag atttggacaa gcgcaatcaa tatttaacaa
     9421 cacaaaataa tcaactcgaa caccatttag acatgttaaa tgcacgtcat caagcgatga
     9481 acgacatggt aagaaacgat acacgtattt tgtttgcggg gcttagatgt gcattgaaag
     9541 ataggaacat tgaattaagt gcagaaatta aatatcaacg tatgccagaa gtcggtattg
     9601 atcccgatac ggtggatcag ggggctttaa cacatgcata tcaaggtgat gtgtcaggga
     9661 tagaccatca tataagaaag gcatcagcac ctattaaaaa agagacagag aatgacttag
     9721 aattagaata aatcaataaa aataagcact caggcaagta tgatacttgt ttgagcgctt
     9781 atctcaatga aaagagtaag taaaaactta ataagctggc gacacttatt aagagcaata
     9841 ataactttaa ttactgaact agtctactaa ttcagtaatt aggaacgata cctttgtatt
     9901 tctatattat tataagaatt ccgaggtagc aatgatattg acttaatatg agcaaattga
     9961 tattggaagc aaaagaaacc cactcaaacg aacgagtggg ttttgaaaaa tgatacgtat
    10021 taacttgata aaattaatca ttttatagtt taaagaaaag gaatgcataa ttatagtaac
    10081 acaaattaat gtttgttaca ctagtagtgc tgctcaatat ataaagtagt aaaccataac
    10141 tattcttcgc atctcttgtt aaaaagttaa ttttttgctt tgtacaagga aaagcactca
    10201 aaattgagtg cttttttcat attaaaatta aactttaaaa attaagacag cgacactgat
    10261 atgtgtcgct atcttaagat atattatatg agttcaaaag atatatagct attgtagcat
    10321 aatagtgttt ttattaaaaa acaagacgaa atagtttgaa ttactgaata acttatgtct
    10381 taaatctttt aatttattta agatacgttg ttattaagaa aataaaaaaa gatataaatc
    10441 aactaaataa ttgatttata tcttcatatg tattgattta aaatacttac tacattaaac
    10501 caaacgcttt agcaacgtct gatattacac caataccatt agctgcaata ttaacaattg
    10561 atttagctaa ttcggcacta tctccagatt gtgatgctga aatagtatta acaatagctt
    10621 cacctaattt tgaaatatgt tccatgaaaa acaaacctcc ttacatataa aatcattact
    10681 caaataaaat aacatatatt acgataattc tcttctactt ttaaataatt gtaattaaat
    10741 ttacgttttc ttaaaaataa ataaagggat tgcaatgttg ttactattaa aattaataaa
    10801 aaaactttga atataataat agctatttta catggaattt tttaatatct ttttataaat
    10861 ctttttataa acctttttag aagtcgtttg agctttagag ccacagctta tttgagatgc
    10921 ttaaggagta gttttgccac ataaaaggag tagttttgcc acataaaagg agtagttttg
    10981 ccacataaaa ggagtagttt tgccacataa aaggagtagt ttttattata aaccttcttt
    11041 tgttaattga cattgttatc aaagtgtttt aacgtgaaaa tatcaattca ataaggagta
    11101 tcataaaaaa atatgctcct tattgaaata attatgttga ggtatctata ta
//
