LOCUS       NZ_EF488047             3195 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Corynebacterium renale plasmid pCR2, complete sequence.
ACCESSION   NZ_EF488047
VERSION     NZ_EF488047.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225706
KEYWORDS    RefSeq.
SOURCE      Corynebacterium renale
  ORGANISM  Corynebacterium renale
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Corynebacteriaceae; Corynebacterium.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Walia,R., Deb,J.K. and Mukherjee,K.J.
  TITLE     Development of expression vectors for Escherichia coli based on the
            pCR2 replicon
  JOURNAL   Microb Cell Fact 6, 14 (2007)
   PUBMED   17490494
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Walia,R. and Deb,J.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2007) Department of Biochemical Engineering and
            Biotechnology, Indian Institute of Biotechnology, Hauz Khas, Delhi,
            Delhi 11006, India
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009951535.1-RS_2024_05_03
            Annotation Date              :: 05/03/2024 03:40:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EF488047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Corynebacterium renale"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1724"
                     /plasmid="pCR2"
     gene            1707..1820
                     /locus_tag="HS454_RS00015"
     CDS             1707..1820
                     /locus_tag="HS454_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071593754.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGN"
                     /name="replication initiation protein"
     gene            complement(2176..2727)
                     /locus_tag="HS454_RS00005"
     CDS             complement(2176..2727)
                     /locus_tag="HS454_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="WP_308118115.1"
                     /translation="MATETTEADAAAIMGILMDIADFLRNTPVWVWILLAFLLRRGFAA
                     LYDREMTTGRLFFLPILFLVWGAYGVITETKLAGVSLTMMTAGLLVGTALGYRLWRSQP
                     PLRNSDNPGMIIRAGTPLTLGLIVITFCMKFILVSVIDLHPGLRSSAIFCMLFGGVTGL
                     VDGVFWGGTLRLFIPWYSKR"
                     /name="DUF6622 family protein"
     gene            complement(2688..3155)
                     /locus_tag="HS454_RS00010"
     CDS             complement(2688..3155)
                     /locus_tag="HS454_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001530439.1"
                     /translation="MSDTNKFRSLRLARAWSQEQLAELSGMSVRTVQRIENGDQPSLET
                     LSALAAVFEVSVADLSGPDAHGDDALDQKIAEAKSKIAEEGRFYRSVITAVVVCALLLV
                     LNHFTAPASAWSFWVAGIWCALLVIRGMRTFVLRGLISRWQQKRLRRMLRR"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1330..1820
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.129"
                     /coverage="94.0"
                     /evalue="1.66e-162"
ORIGIN
        1 accgcgtaat cctgcgcgac cacgatctta acccgacagt aacgtgacag cggtctgaca
       61 tgccgcattg aggtctttga aaccgtaact tcagaagcat gtacggtcag atttaacata
      121 agagttcatt gtacgcaccg ttaaaacgcg ctcagcgcgc ttctggcgca aaaaccgtaa
      181 aaatggatgt tttcccccgg gtaaaccgga aaaatgcgtc aggaacgctt tcagcgcgtt
      241 gcatgactat gcatgaaact gaatggcgat cggtttgggc gcgtctgatg cccataaggc
      301 gtattttcgg acgttttcag ccctgataag aagaaatcag actgtagtta cagacgagtc
      361 gtgagcgatt cactacggga gtcgtcggcg agtcatccag tatttttcct cgcgactctc
      421 tggcgactcg ccttctctga acaccagagc gacagtgtgt tgagtcatcg ataaatcacc
      481 gacgactcgt tgccgagtca tccagtagtc gccgacgagc cgcttttgta taaatccgaa
      541 taagaaaata tatttttcaa accataacaa catgatttaa aaagcaaatc agaaaaaagt
      601 tagttttgcg tggggtgtgg gcatcctggg aatgagaaca gactcgcgtt tttctggagg
      661 aactgcgggg atttttgatt aaacaatagt caccgcagag cggaatttta tgcaacgctg
      721 gctgtgcggc acggggattt ttaatccccc ggcccgttat tcatctccac gggcgacggg
      781 gatacataaa cccgacagca gaggacgggt gagcgcgaat cccagagatg atgaaaaaag
      841 aggcagagaa acgcgcccag gtacgtttta tcttattgct ttggtgttgt ccagggtgtc
      901 ggggctgtgc cctgaccagg tggcatttgt ctgattgcgc gtgcgcggtc cgacaaatgc
      961 acatcctgcc ccgtcctgta cgtgtttttt tcaccagaac aacttcacga agtggcggat
     1021 gaacgctacc aacgttgccg ggaacgcttc ggcgatgatg gcataacggg ctgatacagg
     1081 cagctcccgg agacggacac agcttgcctg tgagcggatg ccgggagccg acaagcccgt
     1141 cagggcgcgt cagcgggttt tagcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     1201 gatagcggag tgtatgctgg cttaatatgt tgacttaata tgttaaatcg gagtggtaat
     1261 tcagggaagt gcttcatgtg gcaaaggaaa aatgtggcta tcgtgcgtaa gtgcaacatg
     1321 taggtaaagg tgaaatgacg cctcctcgct cactcggtcg ctacgctcct gccgtgagac
     1381 tgcggcgggc gttaccggct cacaaataac gggatacgca ggcagtgctc aaatcaggaa
     1441 ggaccggaaa aaggatgcgg cgtagccgtt tttccatagg ctccgccccc ctgacaagca
     1501 tcacgaaatc tgacgctcaa atcagtggcg gcgaaacccg acaggactat aaagatccca
     1561 ggcgtttccc cctggtagct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     1621 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     1681 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactaccacg
     1741 cccgttccgg taactatcaa cttgagtcca acccggaaag acacgacaaa tcgccagtgg
     1801 cggtagccat tggtaactga gatgtgcgag agatttatct ggagttcttg aagtgggggc
     1861 ctgagtgcgg ctacactgga aggacagttt aggtgactcg tctcgcacaa gacagttacc
     1921 aggttaagca gttccccaac tgacctaacc ttcgatcaaa ccacctcccc aggtggtttt
     1981 ttcgttttca gagcaagaga ttacgcgcag aaaaaaagga tctcaagaag atccttttta
     2041 caggagcgat tatcgtcttc atccatgaag gcgtttgaag attaaaccgg cctatttcat
     2101 agatcgtaaa atcagggttt tgggatggcc gatgaaaccc cataaaaacc cataaataca
     2161 tacacctact aacaatcatc ttttgctgta ccagggtatg aaaagtctca gggttccacc
     2221 ccagaatacg ccatcaacaa gtcctgtcac accgccaaat aacatgcaaa aaattgcgga
     2281 tgaccgtaat ccggggtgca gatcaatgac tgagacaagt ataaacttca tgcaaaaagt
     2341 aattacaatc agtcccaaag tcagcggtgt cccggccctg ataatcatgc ccggattatc
     2401 tgaatttctc agcgggggct gtgagcgcca caacctgtat ccaagagcgg tgcctacgag
     2461 cagtcctgcc gtcatcattg taaggcttac gccagcaagt tttgtctcag tgataacacc
     2521 atatgctccc catacaagga aaagtatcgg gagaaaaaac aaacgcccgg ttgtcatctc
     2581 ccggtcataa agagcagcaa aaccgcgtcg tagtaaaaaa gccagcagga tccataccca
     2641 gacgggggta tttcgtaaaa agtctgcaat atccataagt attcccatta tcgccgcagc
     2701 atccgcctca gtcgtttctg ttgccatctg ctgatcagcc cgcgcaaaac gaacgttctc
     2761 atgccacgaa taaccagcag cgcacaccag atccccgcaa cccaaaatga ccatgcgctg
     2821 gccggggccg taaagtggtt aagaacaagc aacagcgcgc agacgacgac agccgtaatt
     2881 actgatcggt aaaaacggcc ttcctcagct attttgctct tcgcttcggc aattttctgg
     2941 tcaagagcgt catcgccatg cgcatcaggg ccggaaagat ccgccacgct cacctcaaat
     3001 acagcagcca gggcactcag tgtttccagg cttggctgat cgccgttctc gatacgctga
     3061 accgtgcgca cgctcatccc ggacagttca gcaagctgct cctgggacca ggcacgcgca
     3121 agacgcagcg acctgaattt gttggtatca ctcatttcct gtctccgaat ggaagatggt
     3181 cagcacacag tgtta
//
