LOCUS       NZ_CP157144            10109 bp    DNA     circular CON 31-MAY-2024
DEFINITION  Escherichia coli strain UTAK-11 plasmid pUTAK-11-4, complete
            sequence.
ACCESSION   NZ_CP157144
VERSION     NZ_CP157144.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41050893
            Assembly: GCF_039911255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Insights into the Plasticity and Virulence of Escherichia coli
            Serogroup O45
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2024) South Texas Center For Emerging Infectious
            Disease, University of Texas at San Antonio, One UTSA Circle, San
            Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 399x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039911255.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 14:24:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,840
            CDSs (total)                      :: 5,709
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 102
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 464
            CDSs (without protein)            :: 464
            Pseudo Genes (ambiguous residues) :: 0 of 464
            Pseudo Genes (frameshifted)       :: 284 of 464
            Pseudo Genes (incomplete)         :: 170 of 464
            Pseudo Genes (internal stop)      :: 68 of 464
            Pseudo Genes (multiple problems)  :: 52 of 464
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP157144.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="UTAK-11"
                     /serotype="O45"
                     /isolation_source="culture"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pUTAK-11-4"
                     /geo_loc_name="USA"
                     /collection_date="2013"
     gene            complement(join(9625..10109,1..27))
                     /locus_tag="ABH538_RS28715"
                     /old_locus_tag="ABH538_28725"
                     /pseudo=""
     CDS             complement(join(9625..10109,1..27))
                     /locus_tag="ABH538_RS28715"
                     /old_locus_tag="ABH538_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(<65..715)
                     /locus_tag="ABH538_RS28720"
                     /old_locus_tag="ABH538_28730"
                     /pseudo=""
     CDS             complement(<65..715)
                     /locus_tag="ABH538_RS28720"
                     /old_locus_tag="ABH538_28730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(705..1028)
                     /locus_tag="ABH538_RS28725"
                     /old_locus_tag="ABH538_28735"
     CDS             complement(705..1028)
                     /locus_tag="ABH538_RS28725"
                     /old_locus_tag="ABH538_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047089054.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1094..1285
                     /locus_tag="ABH538_RS28730"
                     /old_locus_tag="ABH538_28740"
     CDS             1094..1285
                     /locus_tag="ABH538_RS28730"
                     /old_locus_tag="ABH538_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094905248.1"
                     /translation="MTKQEKTALNMARFIRSQTLMLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1437..1655)
                     /locus_tag="ABH538_RS28735"
                     /old_locus_tag="ABH538_28745"
     CDS             complement(1437..1655)
                     /locus_tag="ABH538_RS28735"
                     /old_locus_tag="ABH538_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094905250.1"
                     /translation="MFFPALSPDSVDNRITAFECADTARRSRTTERSESVSEEAEERRK
                     LHFLLTHLCGISHPAWCTFHTICTDAA"
                     /name="hypothetical protein"
     gene            1956..2087
                     /locus_tag="ABH538_RS28740"
                     /old_locus_tag="ABH538_28750"
     CDS             1956..2087
                     /locus_tag="ABH538_RS28740"
                     /old_locus_tag="ABH538_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_249820084.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKGCTRYY"
                     /name="replication initiation protein"
     gene            2452..3510
                     /locus_tag="ABH538_RS28745"
                     /old_locus_tag="ABH538_28755"
     CDS             2452..3510
                     /locus_tag="ABH538_RS28745"
                     /old_locus_tag="ABH538_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_094905242.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVIDLCPQAN
                     SSSMLLGGMVNGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSNYVINVSTYNSR
                     IPDNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWDNGDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHLNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKKSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKNVAGRTIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(3540..3794)
                     /locus_tag="ABH538_RS28750"
                     /old_locus_tag="ABH538_28760"
     CDS             complement(3540..3794)
                     /locus_tag="ABH538_RS28750"
                     /old_locus_tag="ABH538_28760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023259653.1"
                     /translation="MDSNNSQKNLQDKHADLLDKTYTLGWLDGYSEGYDDAIEDCQNAL
                     NKLENENELLNEIIENKDNVISEMASSVGRALQTVKKNK"
                     /name="hypothetical protein"
     gene            complement(3784..3969)
                     /locus_tag="ABH538_RS28755"
                     /old_locus_tag="ABH538_28765"
     CDS             complement(3784..3969)
                     /locus_tag="ABH538_RS28755"
                     /old_locus_tag="ABH538_28765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074518866.1"
                     /translation="MTEDNVKNISDYRKNNDAVDANSSAISGKSAPAKLSEKLRNTVPS
                     GLRAQMRQQMRNKHGF"
                     /name="hypothetical protein"
     gene            complement(4327..4560)
                     /locus_tag="ABH538_RS28760"
                     /old_locus_tag="ABH538_28770"
     CDS             complement(4327..4560)
                     /locus_tag="ABH538_RS28760"
                     /old_locus_tag="ABH538_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4567..5085)
                     /locus_tag="ABH538_RS28765"
                     /old_locus_tag="ABH538_28775"
     CDS             complement(4567..5085)
                     /locus_tag="ABH538_RS28765"
                     /old_locus_tag="ABH538_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140902897.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVLLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5123..5773)
                     /locus_tag="ABH538_RS28770"
                     /old_locus_tag="ABH538_28780"
                     /pseudo=""
     CDS             complement(<5123..5773)
                     /locus_tag="ABH538_RS28770"
                     /old_locus_tag="ABH538_28780"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5763..6086)
                     /locus_tag="ABH538_RS28775"
                     /old_locus_tag="ABH538_28785"
     CDS             complement(5763..6086)
                     /locus_tag="ABH538_RS28775"
                     /old_locus_tag="ABH538_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047089054.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6152..6343
                     /locus_tag="ABH538_RS28780"
                     /old_locus_tag="ABH538_28790"
     CDS             6152..6343
                     /locus_tag="ABH538_RS28780"
                     /old_locus_tag="ABH538_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094905248.1"
                     /translation="MTKQEKTALNMARFIRSQTLMLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6495..6713)
                     /locus_tag="ABH538_RS28785"
                     /old_locus_tag="ABH538_28795"
     CDS             complement(6495..6713)
                     /locus_tag="ABH538_RS28785"
                     /old_locus_tag="ABH538_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094905250.1"
                     /translation="MFFPALSPDSVDNRITAFECADTARRSRTTERSESVSEEAEERRK
                     LHFLLTHLCGISHPAWCTFHTICTDAA"
                     /name="hypothetical protein"
     gene            7014..7145
                     /locus_tag="ABH538_RS28790"
                     /old_locus_tag="ABH538_28800"
     CDS             7014..7145
                     /locus_tag="ABH538_RS28790"
                     /old_locus_tag="ABH538_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_249820084.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKGCTRYY"
                     /name="replication initiation protein"
     gene            7510..8568
                     /locus_tag="ABH538_RS28795"
                     /old_locus_tag="ABH538_28805"
     CDS             7510..8568
                     /locus_tag="ABH538_RS28795"
                     /old_locus_tag="ABH538_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_094905242.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVIDLCPQAN
                     SSSMLLGGMVNGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSNYVINVSTYNSR
                     IPDNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWDNGDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHLNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKKSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKNVAGRTIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(8598..8852)
                     /locus_tag="ABH538_RS28800"
                     /old_locus_tag="ABH538_28810"
     CDS             complement(8598..8852)
                     /locus_tag="ABH538_RS28800"
                     /old_locus_tag="ABH538_28810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023259653.1"
                     /translation="MDSNNSQKNLQDKHADLLDKTYTLGWLDGYSEGYDDAIEDCQNAL
                     NKLENENELLNEIIENKDNVISEMASSVGRALQTVKKNK"
                     /name="hypothetical protein"
     gene            complement(8842..9027)
                     /locus_tag="ABH538_RS28805"
                     /old_locus_tag="ABH538_28815"
     CDS             complement(8842..9027)
                     /locus_tag="ABH538_RS28805"
                     /old_locus_tag="ABH538_28815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074518866.1"
                     /translation="MTEDNVKNISDYRKNNDAVDANSSAISGKSAPAKLSEKLRNTVPS
                     GLRAQMRQQMRNKHGF"
                     /name="hypothetical protein"
     gene            complement(9385..9618)
                     /locus_tag="ABH538_RS28810"
                     /old_locus_tag="ABH538_28820"
     CDS             complement(9385..9618)
                     /locus_tag="ABH538_RS28810"
                     /old_locus_tag="ABH538_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1371..1456)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(6429..6514)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          1482..2028
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.175"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6540..7086
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.175"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..715)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.958"
                     /coverage="100.0"
                     /evalue="4.54e-146"
     Typing          complement(4224..5773)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9282..10108)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.58"
                     /coverage="100.0"
                     /evalue="4.15e-137"
ORIGIN
        1 cgcgcccgtc tgtaaaagat tgctcataaa acgctccttt cagcctgata ttcttcccgc
       61 cgttcgggtc tgcaatgctg atgctgcttc gcgtcaccct gacaacctca actcccacat
      121 ctgtgagtgc cagaatcaca tcctgacggt cttttatctc tccggcacgg taaagcacat
      181 caagcccccg tgtaacgctc tcagcaagcg cctgtttcgt ttcaggcagg ttatcaggga
      241 gtgtcagcgt cctgcggttc tccggggcgt tcgggtcatg cagcccgtaa tggtgattca
      301 ccagcgtctg ccaggcatca attctaggcc tgtctgcgcg gtcgtagtac ggctggaggc
      361 gttttccggt ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt
      421 cctggtgctc cacccacagg atgctgtact gatttttttc gagaccgggc atcagtacac
      481 gctcaaagct cgccatcact ttttcacgtc ctcccgctgg cagttccttc tccgcgaacg
      541 acagaacacc ggacgtgtat ttcttcgcaa atggcgtggc atagatgagt tcccggactt
      601 cttccggatt accctgaagc accgttgccc cttcccggtt acgctccctt cccagcaggt
      661 aatcaaccgg accactgcca ccaccttttc ccctggcatg aaacttaact atcatcccgc
      721 gccccctgtt ccctgacggc cagacgcagc cggcgcagct cctccccgat ggccatcagt
      781 gcggccacca cctgaacccg gtcaccggaa gaccactgcc cgctgttcac cttacgggct
      841 gtctgattca ggttatttcc gatggcggcc agctgacgca gtaacggcgg tgccagtgtc
      901 ggcagcctcc cggaacgggc aaccggctca cccaggcaga cccgccgcat ccataccgcc
      961 agctgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
     1021 gtgagcatcc tctcgcgttt catcggtatc attacccccc atgaacagaa atccccctta
     1081 cacggaggca tcagtgacta aacaggaaaa aaccgccctt aacatggccc gctttatcag
     1141 aagccagaca ttaatgctgc tggagaagct caacgaactg gacgcggatg aacaggctga
     1201 tatctgtgaa tcgctccacg accacgccga tgagctttac cgcagttgcc tcgcacgctt
     1261 cggggatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgcct
     1321 gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtt ttagcgggtg
     1381 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     1441 gcggcatcag tgcagattgt atgaaaagtg caccatgccg ggtgtgaaat gccgcacaga
     1501 tgcgtaagga gaaaatgcaa cttccgacgc tcttccgctt cctcgctcac tgactcgctg
     1561 cgctcggtcg ttcggctgcg gcgagcggtg tctgcgcact caaaagcggt aatacggtta
     1621 tccacagaat caggggataa cgccggaaag aacatgtgag caaaaagacg aagggctaga
     1681 gaaaagccgc gtagatggcg ttttttcata ggctccgccc ccctgacgag catcacaaaa
     1741 accgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1801 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1861 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca
     1921 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1981 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttaa
     2041 cgccactggc agcagccact ggtaagggat gtacaagata ttactaacgc agagttcttg
     2101 aagttgaagc ctaactacag ctaaactgaa aggacagatt tggtggcagt ggtcttgtac
     2161 accctgttac cacggttcag aagttcccca acttactgat ccttcgaaaa accacctccc
     2221 caggtggttt tttcgtttac agtgcagaag attacatccc acgaataagg atctcaagaa
     2281 atcctttcaa tcacgttcct actatgctga agaggtcatg ccaatacgga caccctcatc
     2341 taaccatacc gctcgaaaca cagtcagtta gctgaaagaa actaacagta gaacaataat
     2401 ctctagtact ctgacttaaa gctaacaaca aaactatatt aggagtgata gatggcgatc
     2461 tatgccgtat ggaataacaa aggcggagtg gggaaaagtt atcttacctt tcaactagcc
     2521 tctgagtacg ccagacagaa cccgacaaaa aaggttctgg tgattgatct atgcccacaa
     2581 gctaactcat cctcaatgtt acttggcggg atggttaatg gcgaaaatac gctaaatcag
     2641 attcattctg caacacaaag aaagactatt tctggatata ttgaggatag gatcagaagt
     2701 ccatatattc cgacaaatac aggttctaac tacgtaatca acgtatctac atacaatagc
     2761 agaatcccag ataatctata tcttgttact ggcgatgagc aattagaact tcaagcatca
     2821 agagttgttg gagcatcatt ccctggacct catgacgcat ggagaactgt tcatctttgg
     2881 attaacgatc taattagtga catttgtaac tcatgggata atggcgacag ctgcgttttc
     2941 atcgactgta atcccagctt ctctatttat accgagttag cattaaccgc agctgaacgc
     3001 ttattaattc cattctctgc tgatggttca tcaaaaagag ctgttaaagc agttctttct
     3061 ttagtttatg gcgagcgacg tcatctgaac gaaccacagt cagatttcta tttaaacagc
     3121 aatcgttatt ctatgtcatt accacaaata tacatgtatg ttggaaacag attaactcaa
     3181 atgaacaact catcagctag cgcattcaaa actgtggtaa cagaaatcgg cgatgagatt
     3241 tggaaagtgt ggcaaacaaa accaagttta ttctgcattc atccaaatgg aagctcaacg
     3301 ccgaataata aaaaatcctt taggaacatg ttccagtatg aagtaaatga tgccaataca
     3361 gcatctgtcg tatcaggagc acttggtatt ccaattgcca cactaaccgc aggaaataag
     3421 aatgttgcgg gaagaacaat aaaggtaaat cagacacagc ttgataagca agtgcccaac
     3481 attgtcgctc ttgccggtat gattgagtaa aaaaaaggcc ctttctcaag ggcctttttt
     3541 tatttatttt tctttaccgt ttgcaacgcc ctgcccacac tagatgccat ttcactgatt
     3601 acattatctt tgttttcaat aatttcattg agcaattcgt tttcattttc cagtttattc
     3661 aaggcatttt gacaatcttc aatggcatca tcataccctt ctgaataccc atccaaccag
     3721 ccgagagtat aagttttatc cagaagatca gcatgcttat cttgaaggtt tttctgactg
     3781 ttattagaat ccatgtttat tcctcatttg ttggcgcatt tgggccctca gtccagaagg
     3841 aactgtattt ctaagtttct cactcagctt agctggagcg cttttacctg aaatagcact
     3901 actattagca tcaacagcat cattattctt tctgtagtcg ctaatattct taacgttatc
     3961 ttcagtcata gaacattaca cctattgctg taaacagccc aatatacgct ggagaattga
     4021 acataacacc ccttgtacgc accgctaaaa tgcgttcatc gcgatcacgg cagcagacag
     4081 gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcgcctgata aattttaacc
     4141 gtatgaatac ctatgcaacc agatggtaca ggccacatta ccccctctta atacactgaa
     4201 gctgccattt ttcattgttt caccatccca gcgaagggcc atccagcgtg cgttcctgta
     4261 tttccggctg acgctcccgt tccagggata acgcatattc gcgctcctgt atcaaccgtt
     4321 cctctcttat ctccagttct cgctgtataa ctggctcaag cgttctgtct gctcgctcaa
     4381 gtgctgcacc tgctgactca actgcatgac ccgctcgttc agcatcgcgt tgtcccgttg
     4441 cgtacgcgaa aacatcttct gcaattccac gaaggcgctc tcccattcgc tcagccgctg
     4501 catatagtcc tgttgcagct gctctaaggc gttcagcaaa tgtgtttcca gctctgtcat
     4561 tctgtgtcac tccttcagat gtacccactc tttcccctga aagggaatca cctctgctga
     4621 tttcccgtac ggaaggacca gaaatttcct gttcccgtcc tgcacgaact ccacgcccca
     4681 tgtcttcgcg ttcagtttct gcaatgtctc ttcctgcttc ctgatatctt ccaggttcgc
     4741 ctgtatcctc ccgccaagat accagagcgt cccgccactc gcggtaaaca ggagcaagac
     4801 tatccccagt aacatcatgc ccgtattccc tgccagcttt aacacgtcct tcctgtgctg
     4861 catcatcgcc tctttcaccc cttcccggtg tttttccagc gatttctctg tcgaggctgt
     4921 gaacagggct atagcgtctc tgattttcgt ctcgtttgag gtcacagcct cgctgacaga
     4981 ctcgccgagc ttcctgaact cgtcgttcag cattttctct gtagattcgg ctctctcttt
     5041 cagctttttc tcgaactccg cgcccgtctg taaaagattg ctcataaaac gctcctttca
     5101 gcctgatatt cttcccgccg ttcgggtctg caatgctgat gctgcttcgc gtcaccctga
     5161 caacctcaac tcccacatct gtgagtgcca gaatcacatc ctgacggtct tttatctctc
     5221 cggcacggta aagcacatca agcccccgtg taacgctctc agcaagcgcc tgtttcgttt
     5281 caggcaggtt atcagggagt gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca
     5341 gcccgtaatg gtgattcacc agcgtctgcc aggcatcaat tctaggcctg tctgcgcggt
     5401 cgtagtacgg ctggaggcgt tttccggtct gtagctccat gttcggaatg acaaaattca
     5461 gctcaagccg tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga
     5521 gaccgggcat cagtacacgc tcaaagctcg ccatcacttt ttcacgtcct cccgctggca
     5581 gttccttctc cgcgaacgac agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat
     5641 agatgagttc ccggacttct tccggattac cctgaagcac cgttgcccct tcccggttac
     5701 gctcccttcc cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa
     5761 acttaactat catcccgcgc cccctgttcc ctgacggcca gacgcagccg gcgcagctcc
     5821 tccccgatgg ccatcagtgc ggccaccacc tgaacccggt caccggaaga ccactgcccg
     5881 ctgttcacct tacgggctgt ctgattcagg ttatttccga tggcggccag ctgacgcagt
     5941 aacggcggtg ccagtgtcgg cagcctcccg gaacgggcaa ccggctcacc caggcagacc
     6001 cgccgcatcc ataccgccag ctgtttaccc tcacagcgtt ccagtaaccg ggcatgttca
     6061 tcatcagtaa cccgtatcgt gagcatcctc tcgcgtttca tcggtatcat taccccccat
     6121 gaacagaaat cccccttaca cggaggcatc agtgactaaa caggaaaaaa ccgcccttaa
     6181 catggcccgc tttatcagaa gccagacatt aatgctgctg gagaagctca acgaactgga
     6241 cgcggatgaa caggctgata tctgtgaatc gctccacgac cacgccgatg agctttaccg
     6301 cagttgcctc gcacgcttcg gggatgacgg tgaaaacctc tgacacatgc agctcccgga
     6361 gacggtcaca gcttgcctgt gagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     6421 agcgggtttt agcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     6481 gtatactggc ttaactatgc ggcatcagtg cagattgtat gaaaagtgca ccatgccggg
     6541 tgtgaaatgc cgcacagatg cgtaaggaga aaatgcaact tccgacgctc ttccgcttcc
     6601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtgtc tgcgcactca
     6661 aaagcggtaa tacggttatc cacagaatca ggggataacg ccggaaagaa catgtgagca
     6721 aaaagacgaa gggctagaga aaagccgcgt agatggcgtt ttttcatagg ctccgccccc
     6781 ctgacgagca tcacaaaaac cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6841 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6901 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6961 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7021 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7081 cggtaagaca cgacttaacg ccactggcag cagccactgg taagggatgt acaagatatt
     7141 actaacgcag agttcttgaa gttgaagcct aactacagct aaactgaaag gacagatttg
     7201 gtggcagtgg tcttgtacac cctgttacca cggttcagaa gttccccaac ttactgatcc
     7261 ttcgaaaaac cacctcccca ggtggttttt tcgtttacag tgcagaagat tacatcccac
     7321 gaataaggat ctcaagaaat cctttcaatc acgttcctac tatgctgaag aggtcatgcc
     7381 aatacggaca ccctcatcta accataccgc tcgaaacaca gtcagttagc tgaaagaaac
     7441 taacagtaga acaataatct ctagtactct gacttaaagc taacaacaaa actatattag
     7501 gagtgataga tggcgatcta tgccgtatgg aataacaaag gcggagtggg gaaaagttat
     7561 cttacctttc aactagcctc tgagtacgcc agacagaacc cgacaaaaaa ggttctggtg
     7621 attgatctat gcccacaagc taactcatcc tcaatgttac ttggcgggat ggttaatggc
     7681 gaaaatacgc taaatcagat tcattctgca acacaaagaa agactatttc tggatatatt
     7741 gaggatagga tcagaagtcc atatattccg acaaatacag gttctaacta cgtaatcaac
     7801 gtatctacat acaatagcag aatcccagat aatctatatc ttgttactgg cgatgagcaa
     7861 ttagaacttc aagcatcaag agttgttgga gcatcattcc ctggacctca tgacgcatgg
     7921 agaactgttc atctttggat taacgatcta attagtgaca tttgtaactc atgggataat
     7981 ggcgacagct gcgttttcat cgactgtaat cccagcttct ctatttatac cgagttagca
     8041 ttaaccgcag ctgaacgctt attaattcca ttctctgctg atggttcatc aaaaagagct
     8101 gttaaagcag ttctttcttt agtttatggc gagcgacgtc atctgaacga accacagtca
     8161 gatttctatt taaacagcaa tcgttattct atgtcattac cacaaatata catgtatgtt
     8221 ggaaacagat taactcaaat gaacaactca tcagctagcg cattcaaaac tgtggtaaca
     8281 gaaatcggcg atgagatttg gaaagtgtgg caaacaaaac caagtttatt ctgcattcat
     8341 ccaaatggaa gctcaacgcc gaataataaa aaatccttta ggaacatgtt ccagtatgaa
     8401 gtaaatgatg ccaatacagc atctgtcgta tcaggagcac ttggtattcc aattgccaca
     8461 ctaaccgcag gaaataagaa tgttgcggga agaacaataa aggtaaatca gacacagctt
     8521 gataagcaag tgcccaacat tgtcgctctt gccggtatga ttgagtaaaa aaaaggccct
     8581 ttctcaaggg ccttttttta tttatttttc tttaccgttt gcaacgccct gcccacacta
     8641 gatgccattt cactgattac attatctttg ttttcaataa tttcattgag caattcgttt
     8701 tcattttcca gtttattcaa ggcattttga caatcttcaa tggcatcatc atacccttct
     8761 gaatacccat ccaaccagcc gagagtataa gttttatcca gaagatcagc atgcttatct
     8821 tgaaggtttt tctgactgtt attagaatcc atgtttattc ctcatttgtt ggcgcatttg
     8881 ggccctcagt ccagaaggaa ctgtatttct aagtttctca ctcagcttag ctggagcgct
     8941 tttacctgaa atagcactac tattagcatc aacagcatca ttattctttc tgtagtcgct
     9001 aatattctta acgttatctt cagtcataga acattacacc tattgctgta aacagcccaa
     9061 tatacgctgg agaattgaac ataacacccc ttgtacgcac cgctaaaatg cgttcatcgc
     9121 gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc
     9181 gcctgataaa ttttaaccgt atgaatacct atgcaaccag atggtacagg ccacattacc
     9241 ccctcttaat acactgaagc tgccattttt cattgtttca ccatcccagc gaagggccat
     9301 ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac gcatattcgc
     9361 gctcctgtat caaccgttcc tctcttatct ccagttctcg ctgtataact ggctcaagcg
     9421 ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc gctcgttcag
     9481 catcgcgttg tcccgttgcg tacgcgaaaa catcttctgc aattccacga aggcgctctc
     9541 ccattcgctc agccgctgca tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg
     9601 tgtttccagc tctgtcattc tgtgtcactc cttcagatgt acccactctt tcccctgaaa
     9661 gggaatcacc tctgctgatt tcccgtacgg aaggaccaga aatttcctgt tcccgtcctg
     9721 cacgaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct
     9781 gatatcttcc aggttcgcct gtatcctccc gccaagatac cagagcgtcc cgccactcgc
     9841 ggtaaacagg agcaagacta tccccagtaa catcatgccc gtattccctg ccagctttaa
     9901 cacgtccttc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt tttccagcga
     9961 tttctctgtc gaggctgtga acagggctat agcgtctctg attttcgtct cgtttgaggt
    10021 cacagcctcg ctgacagact cgccgagctt cctgaactcg tcgttcagca ttttctctgt
    10081 agattcggct ctctctttca gctttttct
//
