LOCUS       NZ_CP157121            10552 bp    DNA     circular CON 31-MAY-2024
DEFINITION  Escherichia coli strain UTAK-25 plasmid pUTAK-25-3-pCol, complete
            sequence.
ACCESSION   NZ_CP157121
VERSION     NZ_CP157121.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41050889
            Assembly: GCF_039910405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Insights into the Plasticity and Virulence of Escherichia coli
            Serogroup O45
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2024) South Texas Center For Emerging Infectious
            Disease, University of Texas at San Antonio, One UTSA Circle, San
            Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 287x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039910405.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 14:13:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,743
            CDSs (total)                      :: 5,612
            Genes (coding)                    :: 5,151
            CDSs (with protein)               :: 5,151
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 461
            CDSs (without protein)            :: 461
            Pseudo Genes (ambiguous residues) :: 0 of 461
            Pseudo Genes (frameshifted)       :: 284 of 461
            Pseudo Genes (incomplete)         :: 170 of 461
            Pseudo Genes (internal stop)      :: 65 of 461
            Pseudo Genes (multiple problems)  :: 52 of 461
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP157121.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="UTAK-25"
                     /serotype="O45"
                     /isolation_source="culture"
                     /host="Bos taurus"
                     /db_xref="taxon:562"
                     /plasmid="pUTAK-25-3-pCol"
                     /geo_loc_name="USA"
                     /collection_date="2013"
     gene            complement(<1..1242)
                     /locus_tag="ABH537_RS27825"
                     /old_locus_tag="ABH537_27835"
                     /pseudo=""
     CDS             complement(<1..1242)
                     /locus_tag="ABH537_RS27825"
                     /old_locus_tag="ABH537_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1232..1555)
                     /locus_tag="ABH537_RS27830"
                     /old_locus_tag="ABH537_27840"
     CDS             complement(1232..1555)
                     /locus_tag="ABH537_RS27830"
                     /old_locus_tag="ABH537_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047089054.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1621..1812
                     /locus_tag="ABH537_RS27835"
                     /old_locus_tag="ABH537_27845"
     CDS             1621..1812
                     /locus_tag="ABH537_RS27835"
                     /old_locus_tag="ABH537_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094905248.1"
                     /translation="MTKQEKTALNMARFIRSQTLMLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1964..2182)
                     /locus_tag="ABH537_RS27840"
                     /old_locus_tag="ABH537_27850"
     CDS             complement(1964..2182)
                     /locus_tag="ABH537_RS27840"
                     /old_locus_tag="ABH537_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094905250.1"
                     /translation="MFFPALSPDSVDNRITAFECADTARRSRTTERSESVSEEAEERRK
                     LHFLLTHLCGISHPAWCTFHTICTDAA"
                     /name="hypothetical protein"
     gene            2483..2614
                     /locus_tag="ABH537_RS27845"
                     /old_locus_tag="ABH537_27855"
     CDS             2483..2614
                     /locus_tag="ABH537_RS27845"
                     /old_locus_tag="ABH537_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_249820084.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKGCTRYY"
                     /name="replication initiation protein"
     gene            2979..4037
                     /locus_tag="ABH537_RS27850"
                     /old_locus_tag="ABH537_27860"
     CDS             2979..4037
                     /locus_tag="ABH537_RS27850"
                     /old_locus_tag="ABH537_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_094905242.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVIDLCPQAN
                     SSSMLLGGMVNGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSNYVINVSTYNSR
                     IPDNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWDNGDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHLNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKKSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKNVAGRTIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(4067..4321)
                     /locus_tag="ABH537_RS27855"
                     /old_locus_tag="ABH537_27865"
     CDS             complement(4067..4321)
                     /locus_tag="ABH537_RS27855"
                     /old_locus_tag="ABH537_27865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023259653.1"
                     /translation="MDSNNSQKNLQDKHADLLDKTYTLGWLDGYSEGYDDAIEDCQNAL
                     NKLENENELLNEIIENKDNVISEMASSVGRALQTVKKNK"
                     /name="hypothetical protein"
     gene            complement(4311..4496)
                     /locus_tag="ABH537_RS27860"
                     /old_locus_tag="ABH537_27870"
     CDS             complement(4311..4496)
                     /locus_tag="ABH537_RS27860"
                     /old_locus_tag="ABH537_27870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074518866.1"
                     /translation="MTEDNVKNISDYRKNNDAVDANSSAISGKSAPAKLSEKLRNTVPS
                     GLRAQMRQQMRNKHGF"
                     /name="hypothetical protein"
     gene            complement(4854..5087)
                     /locus_tag="ABH537_RS27865"
                     /old_locus_tag="ABH537_27875"
     CDS             complement(4854..5087)
                     /locus_tag="ABH537_RS27865"
                     /old_locus_tag="ABH537_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5094..5612)
                     /locus_tag="ABH537_RS27870"
                     /old_locus_tag="ABH537_27880"
     CDS             complement(5094..5612)
                     /locus_tag="ABH537_RS27870"
                     /old_locus_tag="ABH537_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140902897.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVLLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5650..6300)
                     /locus_tag="ABH537_RS27875"
                     /old_locus_tag="ABH537_27885"
                     /pseudo=""
     CDS             complement(<5650..6300)
                     /locus_tag="ABH537_RS27875"
                     /old_locus_tag="ABH537_27885"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6290..6613)
                     /locus_tag="ABH537_RS27880"
                     /old_locus_tag="ABH537_27890"
     CDS             complement(6290..6613)
                     /locus_tag="ABH537_RS27880"
                     /old_locus_tag="ABH537_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047089054.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6679..6870
                     /locus_tag="ABH537_RS27885"
                     /old_locus_tag="ABH537_27895"
     CDS             6679..6870
                     /locus_tag="ABH537_RS27885"
                     /old_locus_tag="ABH537_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094905248.1"
                     /translation="MTKQEKTALNMARFIRSQTLMLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7022..7240)
                     /locus_tag="ABH537_RS27890"
                     /old_locus_tag="ABH537_27900"
     CDS             complement(7022..7240)
                     /locus_tag="ABH537_RS27890"
                     /old_locus_tag="ABH537_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094905250.1"
                     /translation="MFFPALSPDSVDNRITAFECADTARRSRTTERSESVSEEAEERRK
                     LHFLLTHLCGISHPAWCTFHTICTDAA"
                     /name="hypothetical protein"
     gene            7541..7672
                     /locus_tag="ABH537_RS27895"
                     /old_locus_tag="ABH537_27905"
     CDS             7541..7672
                     /locus_tag="ABH537_RS27895"
                     /old_locus_tag="ABH537_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_249820084.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKGCTRYY"
                     /name="replication initiation protein"
     gene            8037..9095
                     /locus_tag="ABH537_RS27900"
                     /old_locus_tag="ABH537_27910"
     CDS             8037..9095
                     /locus_tag="ABH537_RS27900"
                     /old_locus_tag="ABH537_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_094905242.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVIDLCPQAN
                     SSSMLLGGMVNGENTLNQIHSATQRKTISGYIEDRIRSPYIPTNTGSNYVINVSTYNSR
                     IPDNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWDNGDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHLNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKKSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKNVAGRTIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(9125..9379)
                     /locus_tag="ABH537_RS27905"
                     /old_locus_tag="ABH537_27915"
     CDS             complement(9125..9379)
                     /locus_tag="ABH537_RS27905"
                     /old_locus_tag="ABH537_27915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023259653.1"
                     /translation="MDSNNSQKNLQDKHADLLDKTYTLGWLDGYSEGYDDAIEDCQNAL
                     NKLENENELLNEIIENKDNVISEMASSVGRALQTVKKNK"
                     /name="hypothetical protein"
     gene            complement(9369..9554)
                     /locus_tag="ABH537_RS27910"
                     /old_locus_tag="ABH537_27920"
     CDS             complement(9369..9554)
                     /locus_tag="ABH537_RS27910"
                     /old_locus_tag="ABH537_27920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074518866.1"
                     /translation="MTEDNVKNISDYRKNNDAVDANSSAISGKSAPAKLSEKLRNTVPS
                     GLRAQMRQQMRNKHGF"
                     /name="hypothetical protein"
     gene            complement(9912..10145)
                     /locus_tag="ABH537_RS27915"
                     /old_locus_tag="ABH537_27925"
     CDS             complement(9912..10145)
                     /locus_tag="ABH537_RS27915"
                     /old_locus_tag="ABH537_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_011117658.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(10152..>10550)
                     /locus_tag="ABH537_RS27920"
                     /old_locus_tag="ABH537_27930"
                     /pseudo=""
     CDS             complement(10152..>10550)
                     /locus_tag="ABH537_RS27920"
                     /old_locus_tag="ABH537_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     Typing          complement(1898..1983)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(6956..7041)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          2009..2555
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.175"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7067..7613
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.175"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..1242)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.961"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4751..6300)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9809..10551)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="100.0"
                     /evalue="4.99e-120"
ORIGIN
        1 ttcagcaaat gtgtttccag ctctgtcatt ctgtgtcact ccttcagatg tacccactct
       61 ttcccctgaa agggaatcac ctctgctgat ttcccgtacg gaaggaccag aaatttcctg
      121 ttcccgtcct gcacgaactc cacgccccat gtcttcgcgt tcagtttctg caatgtctct
      181 tcctgcttcc tgatatcttc caggttcgcc tgtatcctcc cgccaagata ccagagcgtc
      241 ccgccactcg cggtaaacag gagcaagact atccccagta acatcatgcc cgtattccct
      301 gccagcttta acacgtcctt cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt
      361 ttttccagcg atttctctgt cgaggctgtg aacagggcta tagcgtctct gattttcgtc
      421 tcgtttgagg tcacagcctc gctgacagac tcgccgagct tcctgaactc gtcgttcagc
      481 attttctctg tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt
      541 aaaagattgc tcataaaacg ctcctttcag cctgatattc ttcccgccgt tcgggtctgc
      601 aatgctgatg ctgcttcgcg tcaccctgac aacctcaact cccacatctg tgagtgccag
      661 aatcacatcc tgacggtctt ttatctctcc ggcacggtaa agcacatcaa gcccccgtgt
      721 aacgctctca gcaagcgcct gtttcgtttc aggcaggtta tcagggagtg tcagcgtcct
      781 gcggttctcc ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca
      841 ggcatcaatt ctaggcctgt ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg
      901 tagctccatg ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac
      961 ccacaggatg ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc
     1021 catcactttt tcacgtcctc ccgctggcag ttccttctcc gcgaacgaca gaacaccgga
     1081 cgtgtatttc ttcgcaaatg gcgtggcata gatgagttcc cggacttctt ccggattacc
     1141 ctgaagcacc gttgcccctt cccggttacg ctcccttccc agcaggtaat caaccggacc
     1201 actgccacca ccttttcccc tggcatgaaa cttaactatc atcccgcgcc ccctgttccc
     1261 tgacggccag acgcagccgg cgcagctcct ccccgatggc catcagtgcg gccaccacct
     1321 gaacccggtc accggaagac cactgcccgc tgttcacctt acgggctgtc tgattcaggt
     1381 tatttccgat ggcggccagc tgacgcagta acggcggtgc cagtgtcggc agcctcccgg
     1441 aacgggcaac cggctcaccc aggcagaccc gccgcatcca taccgccagc tgtttaccct
     1501 cacagcgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     1561 cgcgtttcat cggtatcatt accccccatg aacagaaatc ccccttacac ggaggcatca
     1621 gtgactaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     1681 atgctgctgg agaagctcaa cgaactggac gcggatgaac aggctgatat ctgtgaatcg
     1741 ctccacgacc acgccgatga gctttaccgc agttgcctcg cacgcttcgg ggatgacggt
     1801 gaaaacctct gacacatgca gctcccggag acggtcacag cttgcctgtg agcggatgcc
     1861 gggagcagac aagcccgtca gggcgcgtca gcgggtttta gcgggtgtcg gggcgcagcc
     1921 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagtgc
     1981 agattgtatg aaaagtgcac catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa
     2041 aatgcaactt ccgacgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2101 ggctgcggcg agcggtgtct gcgcactcaa aagcggtaat acggttatcc acagaatcag
     2161 gggataacgc cggaaagaac atgtgagcaa aaagacgaag ggctagagaa aagccgcgta
     2221 gatggcgttt tttcataggc tccgcccccc tgacgagcat cacaaaaacc gacgctcaag
     2281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2401 ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt
     2461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttaacgc cactggcagc
     2581 agccactggt aagggatgta caagatatta ctaacgcaga gttcttgaag ttgaagccta
     2641 actacagcta aactgaaagg acagatttgg tggcagtggt cttgtacacc ctgttaccac
     2701 ggttcagaag ttccccaact tactgatcct tcgaaaaacc acctccccag gtggtttttt
     2761 cgtttacagt gcagaagatt acatcccacg aataaggatc tcaagaaatc ctttcaatca
     2821 cgttcctact atgctgaaga ggtcatgcca atacggacac cctcatctaa ccataccgct
     2881 cgaaacacag tcagttagct gaaagaaact aacagtagaa caataatctc tagtactctg
     2941 acttaaagct aacaacaaaa ctatattagg agtgatagat ggcgatctat gccgtatgga
     3001 ataacaaagg cggagtgggg aaaagttatc ttacctttca actagcctct gagtacgcca
     3061 gacagaaccc gacaaaaaag gttctggtga ttgatctatg cccacaagct aactcatcct
     3121 caatgttact tggcgggatg gttaatggcg aaaatacgct aaatcagatt cattctgcaa
     3181 cacaaagaaa gactatttct ggatatattg aggataggat cagaagtcca tatattccga
     3241 caaatacagg ttctaactac gtaatcaacg tatctacata caatagcaga atcccagata
     3301 atctatatct tgttactggc gatgagcaat tagaacttca agcatcaaga gttgttggag
     3361 catcattccc tggacctcat gacgcatgga gaactgttca tctttggatt aacgatctaa
     3421 ttagtgacat ttgtaactca tgggataatg gcgacagctg cgttttcatc gactgtaatc
     3481 ccagcttctc tatttatacc gagttagcat taaccgcagc tgaacgctta ttaattccat
     3541 tctctgctga tggttcatca aaaagagctg ttaaagcagt tctttcttta gtttatggcg
     3601 agcgacgtca tctgaacgaa ccacagtcag atttctattt aaacagcaat cgttattcta
     3661 tgtcattacc acaaatatac atgtatgttg gaaacagatt aactcaaatg aacaactcat
     3721 cagctagcgc attcaaaact gtggtaacag aaatcggcga tgagatttgg aaagtgtggc
     3781 aaacaaaacc aagtttattc tgcattcatc caaatggaag ctcaacgccg aataataaaa
     3841 aatcctttag gaacatgttc cagtatgaag taaatgatgc caatacagca tctgtcgtat
     3901 caggagcact tggtattcca attgccacac taaccgcagg aaataagaat gttgcgggaa
     3961 gaacaataaa ggtaaatcag acacagcttg ataagcaagt gcccaacatt gtcgctcttg
     4021 ccggtatgat tgagtaaaaa aaaggccctt tctcaagggc ctttttttat ttatttttct
     4081 ttaccgtttg caacgccctg cccacactag atgccatttc actgattaca ttatctttgt
     4141 tttcaataat ttcattgagc aattcgtttt cattttccag tttattcaag gcattttgac
     4201 aatcttcaat ggcatcatca tacccttctg aatacccatc caaccagccg agagtataag
     4261 ttttatccag aagatcagca tgcttatctt gaaggttttt ctgactgtta ttagaatcca
     4321 tgtttattcc tcatttgttg gcgcatttgg gccctcagtc cagaaggaac tgtatttcta
     4381 agtttctcac tcagcttagc tggagcgctt ttacctgaaa tagcactact attagcatca
     4441 acagcatcat tattctttct gtagtcgcta atattcttaa cgttatcttc agtcatagaa
     4501 cattacacct attgctgtaa acagcccaat atacgctgga gaattgaaca taacacccct
     4561 tgtacgcacc gctaaaatgc gttcatcgcg atcacggcag cagacaggta aaaatggcaa
     4621 caaaccaccc gaaaaactgc cgcgatcgcg cctgataaat tttaaccgta tgaataccta
     4681 tgcaaccaga tggtacaggc cacattaccc cctcttaata cactgaagct gccatttttc
     4741 attgtttcac catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggctgacg
     4801 ctcccgttcc agggataacg catattcgcg ctcctgtatc aaccgttcct ctcttatctc
     4861 cagttctcgc tgtataactg gctcaagcgt tctgtctgct cgctcaagtg ctgcacctgc
     4921 tgactcaact gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt acgcgaaaac
     4981 atcttctgca attccacgaa ggcgctctcc cattcgctca gccgctgcat atagtcctgt
     5041 tgcagctgct ctaaggcgtt cagcaaatgt gtttccagct ctgtcattct gtgtcactcc
     5101 ttcagatgta cccactcttt cccctgaaag ggaatcacct ctgctgattt cccgtacgga
     5161 aggaccagaa atttcctgtt cccgtcctgc acgaactcca cgccccatgt cttcgcgttc
     5221 agtttctgca atgtctcttc ctgcttcctg atatcttcca ggttcgcctg tatcctcccg
     5281 ccaagatacc agagcgtccc gccactcgcg gtaaacagga gcaagactat ccccagtaac
     5341 atcatgcccg tattccctgc cagctttaac acgtccttcc tgtgctgcat catcgcctct
     5401 ttcacccctt cccggtgttt ttccagcgat ttctctgtcg aggctgtgaa cagggctata
     5461 gcgtctctga ttttcgtctc gtttgaggtc acagcctcgc tgacagactc gccgagcttc
     5521 ctgaactcgt cgttcagcat tttctctgta gattcggctc tctctttcag ctttttctcg
     5581 aactccgcgc ccgtctgtaa aagattgctc ataaaacgct cctttcagcc tgatattctt
     5641 cccgccgttc gggtctgcaa tgctgatgct gcttcgcgtc accctgacaa cctcaactcc
     5701 cacatctgtg agtgccagaa tcacatcctg acggtctttt atctctccgg cacggtaaag
     5761 cacatcaagc ccccgtgtaa cgctctcagc aagcgcctgt ttcgtttcag gcaggttatc
     5821 agggagtgtc agcgtcctgc ggttctccgg ggcgttcggg tcatgcagcc cgtaatggtg
     5881 attcaccagc gtctgccagg catcaattct aggcctgtct gcgcggtcgt agtacggctg
     5941 gaggcgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct caagccgtcc
     6001 cttgtcctgg tgctccaccc acaggatgct gtactgattt ttttcgagac cgggcatcag
     6061 tacacgctca aagctcgcca tcactttttc acgtcctccc gctggcagtt ccttctccgc
     6121 gaacgacaga acaccggacg tgtatttctt cgcaaatggc gtggcataga tgagttcccg
     6181 gacttcttcc ggattaccct gaagcaccgt tgccccttcc cggttacgct cccttcccag
     6241 caggtaatca accggaccac tgccaccacc ttttcccctg gcatgaaact taactatcat
     6301 cccgcgcccc ctgttccctg acggccagac gcagccggcg cagctcctcc ccgatggcca
     6361 tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg ttcaccttac
     6421 gggctgtctg attcaggtta tttccgatgg cggccagctg acgcagtaac ggcggtgcca
     6481 gtgtcggcag cctcccggaa cgggcaaccg gctcacccag gcagacccgc cgcatccata
     6541 ccgccagctg tttaccctca cagcgttcca gtaaccgggc atgttcatca tcagtaaccc
     6601 gtatcgtgag catcctctcg cgtttcatcg gtatcattac cccccatgaa cagaaatccc
     6661 ccttacacgg aggcatcagt gactaaacag gaaaaaaccg cccttaacat ggcccgcttt
     6721 atcagaagcc agacattaat gctgctggag aagctcaacg aactggacgc ggatgaacag
     6781 gctgatatct gtgaatcgct ccacgaccac gccgatgagc tttaccgcag ttgcctcgca
     6841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     6901 tgcctgtgag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggttttagc
     6961 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     7021 actatgcggc atcagtgcag attgtatgaa aagtgcacca tgccgggtgt gaaatgccgc
     7081 acagatgcgt aaggagaaaa tgcaacttcc gacgctcttc cgcttcctcg ctcactgact
     7141 cgctgcgctc ggtcgttcgg ctgcggcgag cggtgtctgc gcactcaaaa gcggtaatac
     7201 ggttatccac agaatcaggg gataacgccg gaaagaacat gtgagcaaaa agacgaaggg
     7261 ctagagaaaa gccgcgtaga tggcgttttt tcataggctc cgcccccctg acgagcatca
     7321 caaaaaccga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     7501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7621 cttaacgcca ctggcagcag ccactggtaa gggatgtaca agatattact aacgcagagt
     7681 tcttgaagtt gaagcctaac tacagctaaa ctgaaaggac agatttggtg gcagtggtct
     7741 tgtacaccct gttaccacgg ttcagaagtt ccccaactta ctgatccttc gaaaaaccac
     7801 ctccccaggt ggttttttcg tttacagtgc agaagattac atcccacgaa taaggatctc
     7861 aagaaatcct ttcaatcacg ttcctactat gctgaagagg tcatgccaat acggacaccc
     7921 tcatctaacc ataccgctcg aaacacagtc agttagctga aagaaactaa cagtagaaca
     7981 ataatctcta gtactctgac ttaaagctaa caacaaaact atattaggag tgatagatgg
     8041 cgatctatgc cgtatggaat aacaaaggcg gagtggggaa aagttatctt acctttcaac
     8101 tagcctctga gtacgccaga cagaacccga caaaaaaggt tctggtgatt gatctatgcc
     8161 cacaagctaa ctcatcctca atgttacttg gcgggatggt taatggcgaa aatacgctaa
     8221 atcagattca ttctgcaaca caaagaaaga ctatttctgg atatattgag gataggatca
     8281 gaagtccata tattccgaca aatacaggtt ctaactacgt aatcaacgta tctacataca
     8341 atagcagaat cccagataat ctatatcttg ttactggcga tgagcaatta gaacttcaag
     8401 catcaagagt tgttggagca tcattccctg gacctcatga cgcatggaga actgttcatc
     8461 tttggattaa cgatctaatt agtgacattt gtaactcatg ggataatggc gacagctgcg
     8521 ttttcatcga ctgtaatccc agcttctcta tttataccga gttagcatta accgcagctg
     8581 aacgcttatt aattccattc tctgctgatg gttcatcaaa aagagctgtt aaagcagttc
     8641 tttctttagt ttatggcgag cgacgtcatc tgaacgaacc acagtcagat ttctatttaa
     8701 acagcaatcg ttattctatg tcattaccac aaatatacat gtatgttgga aacagattaa
     8761 ctcaaatgaa caactcatca gctagcgcat tcaaaactgt ggtaacagaa atcggcgatg
     8821 agatttggaa agtgtggcaa acaaaaccaa gtttattctg cattcatcca aatggaagct
     8881 caacgccgaa taataaaaaa tcctttagga acatgttcca gtatgaagta aatgatgcca
     8941 atacagcatc tgtcgtatca ggagcacttg gtattccaat tgccacacta accgcaggaa
     9001 ataagaatgt tgcgggaaga acaataaagg taaatcagac acagcttgat aagcaagtgc
     9061 ccaacattgt cgctcttgcc ggtatgattg agtaaaaaaa aggccctttc tcaagggcct
     9121 ttttttattt atttttcttt accgtttgca acgccctgcc cacactagat gccatttcac
     9181 tgattacatt atctttgttt tcaataattt cattgagcaa ttcgttttca ttttccagtt
     9241 tattcaaggc attttgacaa tcttcaatgg catcatcata cccttctgaa tacccatcca
     9301 accagccgag agtataagtt ttatccagaa gatcagcatg cttatcttga aggtttttct
     9361 gactgttatt agaatccatg tttattcctc atttgttggc gcatttgggc cctcagtcca
     9421 gaaggaactg tatttctaag tttctcactc agcttagctg gagcgctttt acctgaaata
     9481 gcactactat tagcatcaac agcatcatta ttctttctgt agtcgctaat attcttaacg
     9541 ttatcttcag tcatagaaca ttacacctat tgctgtaaac agcccaatat acgctggaga
     9601 attgaacata acaccccttg tacgcaccgc taaaatgcgt tcatcgcgat cacggcagca
     9661 gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcgcc tgataaattt
     9721 taaccgtatg aatacctatg caaccagatg gtacaggcca cattaccccc tcttaataca
     9781 ctgaagctgc catttttcat tgtttcacca tcccagcgaa gggccatcca gcgtgcgttc
     9841 ctgtatttcc ggctgacgct cccgttccag ggataacgca tattcgcgct cctgtatcaa
     9901 ccgttcctct cttatctcca gttctcgctg tataactggc tcaagcgttc tgtctgctcg
     9961 ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc
    10021 cgttgcgtac gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgctcagc
    10081 cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct
    10141 gtcattctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg aatcacctct
    10201 gctgatttcc cgtacggaag gaccagaaat ttcctgttcc cgtcctgcac gaactccacg
    10261 ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat atcttccagg
    10321 ttcgcctgta tcctcccgcc aagataccag agcgtcccgc cactcgcggt aaacaggagc
    10381 aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac gtccttcctg
    10441 tgctgcatca tcgcctcttt caccccttcc cggtgttttt ccagcgattt ctctgtcgag
    10501 gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgaggtcac ag
//
