LOCUS       NZ_CP157118             9328 bp    DNA     circular CON 31-MAY-2024
DEFINITION  Escherichia coli strain UTME-8 plasmid pUTME-8-3-pCol, complete
            sequence.
ACCESSION   NZ_CP157118
VERSION     NZ_CP157118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41050888
            Assembly: GCF_039909535.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Insights into the Plasticity and Virulence of Escherichia coli
            Serogroup O45
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Kalalah,A., Bosilevac,J.M. and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2024) South Texas Center For Emerging Infectious
            Disease, University of Texas at San Antonio, One UTSA Circle, San
            Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 643x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039909535.1-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 14:43:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,968
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 72 of 191
            Pseudo Genes (incomplete)         :: 126 of 191
            Pseudo Genes (internal stop)      :: 34 of 191
            Pseudo Genes (multiple problems)  :: 37 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP157118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="UTME-8"
                     /serotype="O45"
                     /isolation_source="culture"
                     /host="Sus scrofa domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pUTME-8-3-pCol"
                     /geo_loc_name="USA"
                     /collection_date="2020"
     gene            complement(join(8265..9328,1..493))
                     /locus_tag="ABH548_RS24795"
                     /old_locus_tag="ABH548_24770"
     CDS             complement(join(8265..9328,1..493))
                     /locus_tag="ABH548_RS24795"
                     /old_locus_tag="ABH548_24770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_224630669.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAVEEKKPFISSPLKSVAGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHSLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDSLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGCSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(839..1054)
                     /locus_tag="ABH548_RS24800"
                     /old_locus_tag="ABH548_24775"
     CDS             complement(839..1054)
                     /locus_tag="ABH548_RS24800"
                     /old_locus_tag="ABH548_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1058..1543)
                     /locus_tag="ABH548_RS24805"
                     /old_locus_tag="ABH548_24780"
     CDS             complement(1058..1543)
                     /locus_tag="ABH548_RS24805"
                     /old_locus_tag="ABH548_24780"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1578..2282)
                     /locus_tag="ABH548_RS24810"
                     /old_locus_tag="ABH548_24785"
                     /pseudo=""
     CDS             complement(<1578..2282)
                     /locus_tag="ABH548_RS24810"
                     /old_locus_tag="ABH548_24785"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2221..2544)
                     /locus_tag="ABH548_RS24815"
                     /old_locus_tag="ABH548_24790"
     CDS             complement(2221..2544)
                     /locus_tag="ABH548_RS24815"
                     /old_locus_tag="ABH548_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059937.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3601..5157)
                     /locus_tag="ABH548_RS24820"
                     /old_locus_tag="ABH548_24795"
     CDS             complement(3601..5157)
                     /locus_tag="ABH548_RS24820"
                     /old_locus_tag="ABH548_24795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_224630669.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAVEEKKPFISSPLKSVAGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHSLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDSLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGCSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(5503..5718)
                     /locus_tag="ABH548_RS24825"
                     /old_locus_tag="ABH548_24800"
     CDS             complement(5503..5718)
                     /locus_tag="ABH548_RS24825"
                     /old_locus_tag="ABH548_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5722..6207)
                     /locus_tag="ABH548_RS24830"
                     /old_locus_tag="ABH548_24805"
     CDS             complement(5722..6207)
                     /locus_tag="ABH548_RS24830"
                     /old_locus_tag="ABH548_24805"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<6242..6946)
                     /locus_tag="ABH548_RS24835"
                     /old_locus_tag="ABH548_24810"
                     /pseudo=""
     CDS             complement(<6242..6946)
                     /locus_tag="ABH548_RS24835"
                     /old_locus_tag="ABH548_24810"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6885..7208)
                     /locus_tag="ABH548_RS24840"
                     /old_locus_tag="ABH548_24815"
     CDS             complement(6885..7208)
                     /locus_tag="ABH548_RS24840"
                     /old_locus_tag="ABH548_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059937.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2658..2830)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          complement(7322..7494)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          2755..3306
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          7419..7970
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(736..2231)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.982"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2228..2544)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.97e-60"
     Typing          complement(5400..6895)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.982"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6892..7208)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.97e-60"
ORIGIN
        1 ccttatcata aataggatgt gacaacaaaa taatcaaatt cccagcaact gattttaacg
       61 gtgaagatat gaatggtttc ttttcctcaa ccgccaattt gctggcactt gaattcaagc
      121 gggtaccaac taaatcaaga gattctggcg aagtcgccag aacaacagca tcattactaa
      181 caacaagaac tgaattaaac ataccagact gcaatcttaa cctatccgcc tcatctttaa
      241 gtgagtcaat atcattagtt gattttattt tttttgctcc atatgctaat tcaccttgag
      301 ccatttcaat ataaccactc attacatctg acattttcat agcgtacgat gaattagcat
      361 aaagcaaatc tttttctata ttttcgcgct gaaaaaccat aataacggca atcagaaaaa
      421 tagaagttaa caaaatccca ccaacggaca aggttatgac tatcggacga aggtgaggct
      481 ttttaattct cataaaaata cactccatat aataaaaaat gccgcattta ccataacatc
      541 ccttatcagc accgccccgg acgggttcgg aacaaatgcg gcgtcagccc ggtaaaaccg
      601 gcagcaaact ctccgctaaa ccgcgcattc tgcgccagat aaatttcacc cggctgcatg
      661 gctatgcagt cgggtgaatg gctccggctt tcagcgcaga tgtccgccga aagccccgtt
      721 ttatcaggtt atcacagtga agggctgtta tacgtccgct gtcgctgaga tcgctccagc
      781 tccagcgctt tctgatgctg caccacgcgc tcctgctccc ggtgcagctc gacggcattc
      841 agtcgctcga caaccggcgc agctgctcgc tcaaactctg cacctgcctg ctcaagcccg
      901 tgacgcgctc gctcagcgct gcgttctccc gttgcataag accagacatc gtccgccatt
      961 ccgcgaaggc gctctcccac tcgtccagcc ttttcgagta gtcctgctgt agctgctcta
     1021 atgcgctcag caactgtttt tccagctccg tcatgtgcta tttccccgcc agtatcatcc
     1081 agctcgaatc ctctccgaac cgtcccgctt ccgggttcac cttcacgcag cggcgtctct
     1141 gctcgccgca ggtcgagagc tgcacgccgc tgttcctctc tgacagcatg gcctgcgtgc
     1201 tcttctgctc ccggatggtc gtgtagttgt cgagtatctg ctgcccctgc caccacagaa
     1261 tgcccgcgct cgaggcgatc agcagcgcgg agaccaggac gatggtcagc cacgtctggc
     1321 tgaccatgcg caccatgcct ttcgtgcgct ggctcatggc tgaggacagt ttccggtcgt
     1381 ggtcgaggat ggcggcgctg attctcttcg cgctttcgtt cagttccgct tttacagatt
     1441 tctcgtgctc gctgaatgcg gctttcagca tctcgccggt attctgctgc tgcgctttcg
     1501 atttctgctc taagtccttc gccagcgtta aaagactgtt cataaatggc tcccttcagt
     1561 cggatgtttc gccccccgtc cgggtcggca atgctgatac tgctttttgt ggtgcggacc
     1621 acctcaaaac ctgcgctttc cagcgcctca gtgacatcct gacgggtttt cagctccccg
     1681 gatgaggcaa gagcaagcag accgcgtgta atcgcttctg cagcctcctg cttcgcttta
     1741 ggcagtgcag acggtgtgac cagtgcccgc cggttctccg gtgcgttcgg gtcgtgcagc
     1801 cccagtctgc cgttcacgat ggtctgccag gcatcgatgc gtggacggtc ggcgcgatcg
     1861 taatacggct ggagccgtct gccggtcagc agctccgtgt tcgggatcag aaaattcagc
     1921 tcaagccgcc ccttgtcacg atgctccacc cacaggatgc tgtactggtc tttaccaagt
     1981 ccgggcatca gaacccgctc aaagcttgcc atcagctttt cccgctggcc ggaaggtaaa
     2041 tccgcttccg caaacgacag taccccggag gtgtatttct taacgtaagg cgacgcgtca
     2101 atcagctccc ggacttcttc cggcttcccc tgcaggacgg tggcgccttc acgctgccgg
     2161 tctttaccca gcagatagtc caccggcccg gcgccaccgc cgcgaccgcg tggatgaaat
     2221 ttaacgatca tcgtccgttc ctttctcaag aacggcgtgc cgcagccgct caagtccggc
     2281 atcgatggcc atcagcgccg caacaatctg caccctgtca tggccggtac cgccgccggc
     2341 gttaacacgg cgggctatct ggttcaggtt attgcccatg ccggcaagct ggcgcagcag
     2401 cgccggcgag attgagggaa gctttccgga acgtgccggc ttttcgtcca ggcaggtctg
     2461 ccgcatccac gccgccagct gcctgccgtc acagcgctca agcagccgcc ggtgctcgcc
     2521 ctccgtcacc cacatggtga gcatcttgtt ccgtttctct gacaaaccgg cctcctgata
     2581 acgccgggac gggcgggaaa ggctttcccg gtcggcgcgg ggttggacaa gccgcaggcg
     2641 cgtcaggctt ttggcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     2701 gtgtatactg gcttactatg ttggcacgga tgcgagtgtc agtgaagtgc ttcatgtggc
     2761 aggagaaaaa tgcagcagcg gagcgtcagc agaatatgtg atacaggata tattccgctt
     2821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta ccgacttaca
     2881 ggcggggcgg aaatttcctg ggagatgcca ggaagaagct taacagggaa gcgataaggc
     2941 cacggcgaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     3001 ctcaaatcag tggtggcgaa acccgacagg acttaaagat accaggcgtt tccccctggt
     3061 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccgatgtcaa tccgctgtta
     3121 tggccgcgtt tatctcattc cacgcctgac actcggttcc gggaaggcag ttcgctccaa
     3181 gctggactgt atgcacgaac cccccgttca gcccgactac cacgttgtcc cggtaactat
     3241 catcttgagt ccaacccgga acagacacga aaaatcgcca gtggcggtag ccattagtaa
     3301 gaggattcac ggagcggagt agtagagtgt tctacagagt tcttgaagtg gtggcctgac
     3361 tgcggctaca ctggaaggac agttttggta cctgcgctcc gtgaaagcca gttaccttcg
     3421 gaagaaagag ttggtagctc tgaattccgg caaacgaacc accgttggta gcggtggttt
     3481 ttttgtttac agggtatgag attacgacga catcgaaggg ataacaagag gatccgcttc
     3541 ttgcccactc ttcgccacaa aaaaaacgac acactagcgc gtaaagcatc atcaaaaaac
     3601 tcaaatagcg ctatatacaa tcactttatt acgcccctcc cttttggctt gataaagagc
     3661 atcgtcagca tttttcagca aaaaatcgat cctaccaaga ggatcatctt gtgttgccac
     3721 accagcagat atggtgaccc gcaagttatt agggaagaca gtactttcaa taacactcct
     3781 aatacgctcc gcaacacact tagctgttac cacagatgta ttcggcagga aaacaacaaa
     3841 ctcttcgcca ccaaaccgac aaacaacatc ttcactacgg caacaggaat taataatatg
     3901 agccaaagaa acaagcacct catctccgac cccatgcccg aaactgtcat ttatctcttt
     3961 aaaaaaatca acatcaatta caataactga acaccctgaa tcccgcttat gattctgtat
     4021 tttacttaaa aaccccaatc tattattcac gccggtaagg ggatctttaa gcgccacctc
     4081 atttagagaa tcaactcttt ccatcattac cttaatatac gtaaaaagcg cctttttaag
     4141 cctatcagct tcggcatacc aagcattgat agctttaata taaactagcg aagctccgat
     4201 atctttagca ttggtagata tagcaagctt ttctaaagga agtgaaatac tggaggcaat
     4261 gaaatatgaa ataagtgaaa acacaataat tattataatt agcagaacaa gtacactttt
     4321 agcataagca ataagaatag tagtcacact gtcagcacga ctataaacca acacattcca
     4381 agcggtattt ttcatactag cataaccaag aagatatcta tgccccgaag aaaaaaatgc
     4441 cccctcacct ctatcagacg cagttaactt tgatttcaaa tcatcagaca taaccatagg
     4501 ccgatcaaca gcacttttat cgttattaaa gatcacattc ccatcatcac taacaatgct
     4561 aatttcagtg ttgttcttga aaaaatgcct gctcaaaaca tcgccaaata aactatgttt
     4621 ttttagatat atagacccac cgacataccc tatgtaattg ccacccttat cataaatagg
     4681 atgtgacaac aaaataatca aattcccagc aactgatttt aacggtgaag atatgaatgg
     4741 tttcttttcc tcaaccgcca atttgctggc acttgaattc aagcgggtac caactaaatc
     4801 aagagattct ggcgaagtcg ccagaacaac agcatcatta ctaacaacaa gaactgaatt
     4861 aaacatacca gactgcaatc ttaacctatc cgcctcatct ttaagtgagt caatatcatt
     4921 agttgatttt attttttttg ctccatatgc taattcacct tgagccattt caatataacc
     4981 actcattaca tctgacattt tcatagcgta cgatgaatta gcataaagca aatctttttc
     5041 tatattttcg cgctgaaaaa ccataataac ggcaatcaga aaaatagaag ttaacaaaat
     5101 cccaccaacg gacaaggtta tgactatcgg acgaaggtga ggctttttaa ttctcataaa
     5161 aatacactcc atataataaa aaatgccgca tttaccataa catcccttat cagcaccgcc
     5221 ccggacgggt tcggaacaaa tgcggcgtca gcccggtaaa accggcagca aactctccgc
     5281 taaaccgcgc attctgcgcc agataaattt cacccggctg catggctatg cagtcgggtg
     5341 aatggctccg gctttcagcg cagatgtccg ccgaaagccc cgttttatca ggttatcaca
     5401 gtgaagggct gttatacgtc cgctgtcgct gagatcgctc cagctccagc gctttctgat
     5461 gctgcaccac gcgctcctgc tcccggtgca gctcgacggc attcagtcgc tcgacaaccg
     5521 gcgcagctgc tcgctcaaac tctgcacctg cctgctcaag cccgtgacgc gctcgctcag
     5581 cgctgcgttc tcccgttgca taagaccaga catcgtccgc cattccgcga aggcgctctc
     5641 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     5701 tttttccagc tccgtcatgt gctatttccc cgccagtatc atccagctcg aatcctctcc
     5761 gaaccgtccc gcttccgggt tcaccttcac gcagcggcgt ctctgctcgc cgcaggtcga
     5821 gagctgcacg ccgctgttcc tctctgacag catggcctgc gtgctcttct gctcccggat
     5881 ggtcgtgtag ttgtcgagta tctgctgccc ctgccaccac agaatgcccg cgctcgaggc
     5941 gatcagcagc gcggagacca ggacgatggt cagccacgtc tggctgacca tgcgcaccat
     6001 gcctttcgtg cgctggctca tggctgagga cagtttccgg tcgtggtcga ggatggcggc
     6061 gctgattctc ttcgcgcttt cgttcagttc cgcttttaca gatttctcgt gctcgctgaa
     6121 tgcggctttc agcatctcgc cggtattctg ctgctgcgct ttcgatttct gctctaagtc
     6181 cttcgccagc gttaaaagac tgttcataaa tggctccctt cagtcggatg tttcgccccc
     6241 cgtccgggtc ggcaatgctg atactgcttt ttgtggtgcg gaccacctca aaacctgcgc
     6301 tttccagcgc ctcagtgaca tcctgacggg ttttcagctc cccggatgag gcaagagcaa
     6361 gcagaccgcg tgtaatcgct tctgcagcct cctgcttcgc tttaggcagt gcagacggtg
     6421 tgaccagtgc ccgccggttc tccggtgcgt tcgggtcgtg cagccccagt ctgccgttca
     6481 cgatggtctg ccaggcatcg atgcgtggac ggtcggcgcg atcgtaatac ggctggagcc
     6541 gtctgccggt cagcagctcc gtgttcggga tcagaaaatt cagctcaagc cgccccttgt
     6601 cacgatgctc cacccacagg atgctgtact ggtctttacc aagtccgggc atcagaaccc
     6661 gctcaaagct tgccatcagc ttttcccgct ggccggaagg taaatccgct tccgcaaacg
     6721 acagtacccc ggaggtgtat ttcttaacgt aaggcgacgc gtcaatcagc tcccggactt
     6781 cttccggctt cccctgcagg acggtggcgc cttcacgctg ccggtcttta cccagcagat
     6841 agtccaccgg cccggcgcca ccgccgcgac cgcgtggatg aaatttaacg atcatcgtcc
     6901 gttcctttct caagaacggc gtgccgcagc cgctcaagtc cggcatcgat ggccatcagc
     6961 gccgcaacaa tctgcaccct gtcatggccg gtaccgccgc cggcgttaac acggcgggct
     7021 atctggttca ggttattgcc catgccggca agctggcgca gcagcgccgg cgagattgag
     7081 ggaagctttc cggaacgtgc cggcttttcg tccaggcagg tctgccgcat ccacgccgcc
     7141 agctgcctgc cgtcacagcg ctcaagcagc cgccggtgct cgccctccgt cacccacatg
     7201 gtgagcatct tgttccgttt ctctgacaaa ccggcctcct gataacgccg ggacgggcgg
     7261 gaaaggcttt cccggtcggc gcggggttgg acaagccgca ggcgcgtcag gcttttggcg
     7321 ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttac
     7381 tatgttggca cggatgcgag tgtcagtgaa gtgcttcatg tggcaggaga aaaatgcagc
     7441 agcggagcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     7501 gctgcgctcg gtcgttcggc tgcggcgagc ggtaccgact tacaggcggg gcggaaattt
     7561 cctgggagat gccaggaaga agcttaacag ggaagcgata aggccacggc gaagccgttt
     7621 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     7681 cgaaacccga caggacttaa agataccagg cgtttccccc tggtggctcc ctcgtgcgct
     7741 ctcctgttcc tgcctttcgg tttaccgatg tcaatccgct gttatggccg cgtttatctc
     7801 attccacgcc tgacactcgg ttccgggaag gcagttcgct ccaagctgga ctgtatgcac
     7861 gaaccccccg ttcagcccga ctaccacgtt gtcccggtaa ctatcatctt gagtccaacc
     7921 cggaacagac acgaaaaatc gccagtggcg gtagccatta gtaagaggat tcacggagcg
     7981 gagtagtaga gtgttctaca gagttcttga agtggtggcc tgactgcggc tacactggaa
     8041 ggacagtttt ggtacctgcg ctccgtgaaa gccagttacc ttcggaagaa agagttggta
     8101 gctctgaatt ccggcaaacg aaccaccgtt ggtagcggtg gtttttttgt ttacagggta
     8161 tgagattacg acgacatcga agggataaca agaggatccg cttcttgccc actcttcgcc
     8221 acaaaaaaaa cgacacacta gcgcgtaaag catcatcaaa aaactcaaat agcgctatat
     8281 acaatcactt tattacgccc ctcccttttg gcttgataaa gagcatcgtc agcatttttc
     8341 agcaaaaaat cgatcctacc aagaggatca tcttgtgttg ccacaccagc agatatggtg
     8401 acccgcaagt tattagggaa gacagtactt tcaataacac tcctaatacg ctccgcaaca
     8461 cacttagctg ttaccacaga tgtattcggc aggaaaacaa caaactcttc gccaccaaac
     8521 cgacaaacaa catcttcact acggcaacag gaattaataa tatgagccaa agaaacaagc
     8581 acctcatctc cgaccccatg cccgaaactg tcatttatct ctttaaaaaa atcaacatca
     8641 attacaataa ctgaacaccc tgaatcccgc ttatgattct gtattttact taaaaacccc
     8701 aatctattat tcacgccggt aaggggatct ttaagcgcca cctcatttag agaatcaact
     8761 ctttccatca ttaccttaat atacgtaaaa agcgcctttt taagcctatc agcttcggca
     8821 taccaagcat tgatagcttt aatataaact agcgaagctc cgatatcttt agcattggta
     8881 gatatagcaa gcttttctaa aggaagtgaa atactggagg caatgaaata tgaaataagt
     8941 gaaaacacaa taattattat aattagcaga acaagtacac ttttagcata agcaataaga
     9001 atagtagtca cactgtcagc acgactataa accaacacat tccaagcggt atttttcata
     9061 ctagcataac caagaagata tctatgcccc gaagaaaaaa atgccccctc acctctatca
     9121 gacgcagtta actttgattt caaatcatca gacataacca taggccgatc aacagcactt
     9181 ttatcgttat taaagatcac attcccatca tcactaacaa tgctaatttc agtgttgttc
     9241 ttgaaaaaat gcctgctcaa aacatcgcca aataaactat gtttttttag atatatagac
     9301 ccaccgacat accctatgta attgccac
//
