LOCUS       NZ_CP156721             4802 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Escherichia coli strain EC596 plasmid pEC596-4, complete sequence.
ACCESSION   NZ_CP156721
VERSION     NZ_CP156721.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41374118
            Assembly: GCF_039851985.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Huang,J.
  TITLE     To the best of our knowledge, this is the first report of ST877,
            with blaNDM-5 and tet(X4)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2024) Department of Clinical Laboratory, Jinhua
            Hospital of Zhejiang University, Mingyue Street 351, Jinhua 321000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039851985.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 14:56:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,600
            CDSs (total)                      :: 4,484
            Genes (coding)                    :: 4,298
            CDSs (with protein)               :: 4,298
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 45 of 186
            Pseudo Genes (incomplete)         :: 131 of 186
            Pseudo Genes (internal stop)      :: 35 of 186
            Pseudo Genes (multiple problems)  :: 24 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP156721.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC596"
                     /isolation_source="drainage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC596-4"
                     /geo_loc_name="China:Jinhua"
                     /lat_lon="29.08 N 119.65 E"
                     /collection_date="2021-08-15"
                     /collected_by="Jinhua Hospital of Zhejiang
                     UniversityPP01-1"
     gene            complement(180..611)
                     /locus_tag="ABEF00_RS22975"
     CDS             complement(180..611)
                     /locus_tag="ABEF00_RS22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024249395.1"
                     /translation="MARVSISEAARLACVSRPTIYKLIKSGELSYTSVVKHGKAVKVID
                     TTELIRVFGSLSFDNERDHAVVKFDDRSTGVNSDFLQNLQHQVALLQAENAGLKDAVNA
                     RDEHINSLRQAMQLLEHKQGTADPQHVRAKSWWQFWKFR"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(611..802)
                     /locus_tag="ABEF00_RS22980"
     CDS             complement(611..802)
                     /locus_tag="ABEF00_RS22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021543809.1"
                     /translation="MTKPQKTALNMAKFIKGQTLLLLEKLNELELDAEADLCEKLHEDA
                     ELLCRTLTIRLDGHQGDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1223..1336
                     /locus_tag="ABEF00_RS22985"
     CDS             1223..1336
                     /locus_tag="ABEF00_RS22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077694381.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDLSPLAAATSN"
                     /name="replication initiation protein"
     gene            1572..2960
                     /locus_tag="ABEF00_RS22990"
     CDS             1572..2960
                     /locus_tag="ABEF00_RS22990"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_024475243.1"
                     /translation="MKIKVENLGFISSGEIDIKDLTIIFGKNNVGKTYLSYATYGILSR
                     LSSLSEGSTIVSKSDFNKLKADGSISIDTSKVASKEIIQKVNKDFTKSLSSFFNAKEDS
                     FKDANIEIEPNFTDSDLLKLSFSSNLNLDGTGVYSFNKSKNSSEIVISFVKLPEEATNK
                     SVGFAFGEDTIASILSSITSNLILKNIFKGLSRPFVITSERTGISLFYKEMDSHRSSLL
                     NHWITSKSSMSTPINKKLSAYSTPISDNINIIRQYESLTKRKSFLYSKRSKRSNRLMTE
                     TLESMLSGSFKHANGETIFTKKEDDKDVSIPMSISSSSVKSLFLLDLYIKHVAQYGDFL
                     VIDEPELNLHPENQVRMAELIVRLINLGIKIIMTTHSDYIIKEINNRIMLSNISKKENL
                     YDLSFRDEDVLRQDRVSAFTICDDRKIKEIQVDKYGINQEIFDDVISSLNERSDLIYDA
                     LCQE"
                     /name="AAA family ATPase"
     gene            2974..3570
                     /locus_tag="ABEF00_RS22995"
     CDS             2974..3570
                     /locus_tag="ABEF00_RS22995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024249394.1"
                     /translation="MISAIKKVIDDRVKIEIIKTANDHELVLKEESDGSDIEELRIINV
                     PEESFAFTLDYTSTSGSLKNIRLFKKLSCYVSSKNEDGINKSCDLVIVTKRDDGVKVVV
                     LDLKSKRTSGKRPTIQIENSILFLNYIFSLIDFYYKEEIGIAIDDIKYIKALVTTGPTQ
                     KTTVSKRPSKSSDVKKIPVKAMHKKATIRYSSIAA"
                     /name="hypothetical protein"
     gene            complement(3614..3745)
                     /locus_tag="ABEF00_RS23000"
     CDS             complement(3614..3745)
                     /locus_tag="ABEF00_RS23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306295021.1"
                     /translation="MQRAENDPDAFFWFSLDKASLFAVFAAESRSARFCGARNETLW"
                     /name="hypothetical protein"
     Typing          985..1340
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="82.0"
                     /evalue="1.79e-151"
     Typing          4368..4623
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.538"
                     /coverage="99.0"
                     /evalue="6.900000000000001e-74"
ORIGIN
        1 atatgcattt gatagtgttg aatttgcaag aatcggcggc gattttacgg tcgacaaaag
       61 agcaatgcag gaatatcgac tccaactcaa tgctcttaat gtgggaaaat ttccgttttc
      121 caaattaccc caaactgagt ggcagaccga acgctatggc tgggatgcgt ggtaatggct
      181 caacggaatt tccaaaactg ccaccaggac tttgctcgga catgctgcgg atcagcagtg
      241 ccctgcttat gctccagtag ctgcatggcc tgacgaagtg aattgatatg ttcgtcccgg
      301 gcattcactg catctttcag cccggcgttt tctgcctgca aaagtgctac ctgatgctgt
      361 aaattctgta aaaaatcact gttaacacct gtcgagcggt cgtcaaattt tacaacagca
      421 tggtcacgtt cattgtcaaa agaaagagaa ccaaaaacac gaataagctc tgtagtatca
      481 ataactttta cagctttccc atgctttaca acagaggtgt aacttaactc accagattta
      541 attaacttgt aaatagtcgg acgactgaca caagcaaggc gtgccgcttc gctaatagat
      601 accctggcca ttatagatcc ccctgatgac catccagacg aatagtcaga gtgcgacaga
      661 gtagttccgc atcctcatgg agcttttcgc aaaggtccgc ctcagcatcg agctcgagtt
      721 cattaagttt ttccagcaaa agcagggtct gccccttaat aaacttcgcc atattaagcg
      781 cagttttctg tggtttcgtc atcacagcgt cagtgctcca tgttaactct ggttcttttc
      841 cgcttcctcg accgttcgct cgctacgctc ggtcacttgt ctgcggcgag cggacggggc
      901 ttacggagaa gcaggtaaga gagtagatga tggacgtaag gaaagggcag ccaaaaagca
      961 tgagaaggcc acgccccctg ggcgttttac cataggctcc gcccccctga cgagcatcac
     1021 agaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
     1081 tttccccctg gtggctccct cgtgcgctct cctgttcccg cctttcggtt taccggtgtc
     1141 attccgcagt tatggccgcg tttatctcat tccacgcctg acactcagtt ccgggtaggc
     1201 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt
     1261 atccggtaac tatcatcttg agtccaaccc ggaaaggcac gacttatcgc cactggcagc
     1321 agccactagt aactgattta gaggagttag tcttgaagtt atgcgccggt taaggctaaa
     1381 ctggaagaac aagttttggt gactgtgctc ctccaagcca gttacctcgg ttcaaaaagc
     1441 gccaactctt tgaaccctcg aaaaaccgcc cgccaaggcg gttttttcgt ttcgatgaag
     1501 attatccttc aataagctca ctcgtcaaga taacattcca cactggggat acattagccg
     1561 cagaggcaaa gatgaaaatt aaagtagaaa atttaggatt tatctcttca ggtgaaatag
     1621 acataaaaga ccttacgatt atctttggta aaaataacgt aggaaaaact tatcttagtt
     1681 atgccactta cggaatatta agccgattga gtagtttatc tgaaggttca actatcgtat
     1741 caaaatcaga ctttaataaa ctaaaagcag atggctctat atcaatcgat acatctaaag
     1801 ttgcatcgaa agagataatc caaaaagtta ataaagattt cacaaagtca ctctcttcat
     1861 tttttaacgc aaaagaagat agttttaaag atgccaatat agaaatagaa ccaaatttca
     1921 cagatagcga tttattaaaa cttagttttt cttccaattt aaatctggat gggacaggag
     1981 tctactcgtt taataagagt aagaactctt ctgagattgt tatttcattt gtaaaactac
     2041 ctgaagaggc tacaaataag tcggtaggtt ttgcttttgg tgaagatacg atagcaagca
     2101 tactatcatc aataacaagc aacttaattc taaagaacat attcaaagga ttgtctaggc
     2161 ctttcgtaat aacatcagaa agaacaggta tttctttatt ttataaggag atggattcgc
     2221 atagaagttc attacttaat cattggataa cttcaaaatc aagcatgtct acgccaataa
     2281 ataaaaaact atctgcgtac tcaaccccta taagcgataa tataaatatc attaggcaat
     2341 acgaaagcct aaccaaaaga aagagttttt tatattcgaa aaggtctaaa agatctaatc
     2401 gtttaatgac cgagacctta gaaagcatgc tttccggttc atttaagcac gccaatggcg
     2461 aaactatttt cacaaaaaaa gaagatgaca aagatgtaag cattccaatg tctatatcgt
     2521 catcttcagt taaatcattg tttttacttg atttatacat aaaacatgtt gctcaatatg
     2581 gcgatttcct tgtgatcgat gagccagaat taaacttgca cccagaaaat caagtgagaa
     2641 tggctgaatt gatagtaaga ctgataaact tagggataaa aataatcatg acaacacata
     2701 gtgattatat tattaaggaa atcaacaata gaataatgct aagcaatatt agcaaaaaag
     2761 aaaatcttta tgatttatca tttagagatg aagatgtttt gcgccaagat agagttagcg
     2821 catttaccat ttgtgatgac agaaaaataa aagaaataca agtagacaag tatgggataa
     2881 atcaggagat atttgatgat gtaatttcat ctctcaatga gagaagtgat cttatatacg
     2941 acgcactatg tcaggaataa tcggatattg tatatgatta gcgcaataaa gaaagttata
     3001 gacgataggg ttaaaataga aataataaaa acggcaaatg accatgagct agtgttaaaa
     3061 gaagaaagtg atggtagtga tattgaagaa ctcaggataa taaatgttcc tgaggagtca
     3121 tttgcattta ctttggatta tacatcaact agtggctccc ttaaaaatat tcgtttgttc
     3181 aaaaaactat cttgttatgt cagttcaaaa aatgaagatg ggataaataa atcctgcgat
     3241 ctggtcatag ttacaaagag agatgacgga gtaaaggttg tagttctaga tttaaaatca
     3301 aaaaggacat ctggaaaaag accaactatt cagatagaaa actctatact ttttttaaac
     3361 tatatttttt ctttgattga tttttattat aaggaagaaa ttggaattgc tattgatgat
     3421 attaaatata taaaggcatt agtcacaaca gggccaactc aaaaaacaac tgtgtctaaa
     3481 agaccgtcaa aatctagtga cgtaaagaaa attcctgtta aggcaatgca taaaaaagca
     3541 acgattcgat atagcagcat agccgcataa acctatggaa agtgagggca acctcacttt
     3601 ccaatcatct tatttaccat aacgtctcat tacgcgcacc gcagaaacgc gctgaacgcg
     3661 attctgccgc aaacaccgca aaaagagatg ctttatccag tgaaaaccag aaaaatgcgt
     3721 caggatcgtt ttcagctcgc tgcatagcta tgcatgagag tgaatggcga acggtttggg
     3781 tgcatacagt gcccatgagg cacattttca gacattttca gttcgtgaat tccaagcatt
     3841 ttttacagga cacccctagg ggcgctgcta ggggtgtcct gtaaaaagat gcgacgcgcc
     3901 cctagggacg accctacatt aattaactgt tttttataaa gattgtcagt actgacccta
     3961 agtagggtcg tcatagcatc gccatagggt cgtcaacgac gtgcttttgc ataaatctga
     4021 aaaagaaaat acatttttaa aacaataaca tcatgattta aaaagaaaat cagcaaaaag
     4081 ttagttttgc gtggggtgtg ggcatttcag gcatgagaac agactcgcgt ttttccgggg
     4141 tatctgcgga atttttgatt aaaaaaacat cgacgcagag cgaaatttta tgcaacagcg
     4201 gctgtactgc acagggatta tttatcagtt gatatgctgt gtcatgatac tacctgaaga
     4261 aacaggacag gatgtttaat tgtaataccg gcacacacaa cgtattgcca cttggtcaga
     4321 gtttccccca caacatcagt gcgaacgtgc ttaatgctcg ctacggctct ctcatgttgc
     4381 cggtgattcc gttgttgttc tttcaggaat gagtcatcag atagccccag aaactcgctc
     4441 agaagcactc agacgcgtga aacgggtttt atatgcccgt cagggcatgg aaggcgcgaa
     4501 taacgcttac agagcgtttc aggaggtcag gcgcgccgat acctaactag gggatttcat
     4561 gtcgctggga accgagtcat cccctgcaaa gccaattcgc acggaacagt aagccactcg
     4621 gttggtagat ggtcgtagac cgtcagtgct acagcaatgg gggtctcccc agacggtttc
     4681 ctgttgcgct caaggctctg ccggaaccgt agcggcaagg ataaaaaacc ctgcgcaatt
     4741 cccgcagggg aaggtgaaaa taactaccta tctcttacgt ttgagttttg aactcagact
     4801 ga
//
