LOCUS       NZ_CP155716            10133 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 578 plasmid p578-3, complete sequence.
ACCESSION   NZ_CP155716
VERSION     NZ_CP155716.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41258466
            Assembly: GCF_039723445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Kuo,P.Y.
  TITLE     urinary tract infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   Kuo,P.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2024) College of Life Sciences, National Yang Ming
            Chiao Tung University, Linong Street, Taipei 112, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039723445.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 13:43:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,753
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,475
            CDSs (with protein)               :: 5,475
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 66 of 157
            Pseudo Genes (incomplete)         :: 97 of 157
            Pseudo Genes (internal stop)      :: 35 of 157
            Pseudo Genes (multiple problems)  :: 35 of 157
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP155716.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="578"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p578-3"
                     /geo_loc_name="Taiwan"
                     /lat_lon="23.00254450 N 120.21938242 E"
                     /collection_date="2009-09-24"
                     /collected_by="NCKUH"
     gene            1..264
                     /locus_tag="ABDC58_RS01005"
                     /old_locus_tag="ABDC58_01005"
     CDS             1..264
                     /locus_tag="ABDC58_RS01005"
                     /old_locus_tag="ABDC58_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            254..553
                     /locus_tag="ABDC58_RS01010"
                     /old_locus_tag="ABDC58_01010"
     CDS             254..553
                     /locus_tag="ABDC58_RS01010"
                     /old_locus_tag="ABDC58_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(615..992)
                     /locus_tag="ABDC58_RS01015"
                     /old_locus_tag="ABDC58_01015"
     CDS             complement(615..992)
                     /locus_tag="ABDC58_RS01015"
                     /old_locus_tag="ABDC58_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            1062..1241
                     /locus_tag="ABDC58_RS01020"
                     /old_locus_tag="ABDC58_01020"
     CDS             1062..1241
                     /locus_tag="ABDC58_RS01020"
                     /old_locus_tag="ABDC58_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1269..1799)
                     /locus_tag="ABDC58_RS01025"
                     /old_locus_tag="ABDC58_01025"
     CDS             complement(1269..1799)
                     /locus_tag="ABDC58_RS01025"
                     /old_locus_tag="ABDC58_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1806..2537)
                     /locus_tag="ABDC58_RS01030"
                     /old_locus_tag="ABDC58_01030"
     CDS             complement(1806..2537)
                     /locus_tag="ABDC58_RS01030"
                     /old_locus_tag="ABDC58_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(2537..4498)
                     /locus_tag="ABDC58_RS01035"
                     /old_locus_tag="ABDC58_01035"
     CDS             complement(2537..4498)
                     /locus_tag="ABDC58_RS01035"
                     /old_locus_tag="ABDC58_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6069..6218)
                     /locus_tag="ABDC58_RS01040"
                     /old_locus_tag="ABDC58_01040"
     CDS             complement(6069..6218)
                     /locus_tag="ABDC58_RS01040"
                     /old_locus_tag="ABDC58_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_020956931.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6303..6560)
                     /locus_tag="ABDC58_RS01045"
                     /old_locus_tag="ABDC58_01045"
     CDS             complement(6303..6560)
                     /locus_tag="ABDC58_RS01045"
                     /old_locus_tag="ABDC58_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_041937905.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKPWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(6570..8258)
                     /locus_tag="ABDC58_RS01050"
                     /old_locus_tag="ABDC58_01050"
     CDS             complement(6570..8258)
                     /locus_tag="ABDC58_RS01050"
                     /old_locus_tag="ABDC58_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_020956933.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKVY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(8843..9361)
                     /locus_tag="ABDC58_RS01055"
                     /old_locus_tag="ABDC58_01055"
     CDS             complement(8843..9361)
                     /locus_tag="ABDC58_RS01055"
                     /old_locus_tag="ABDC58_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956924.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     BGC             1570..10133
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5388..5966
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1810..2537)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.14e-141"
ORIGIN
        1 atgagcaaca cgacacacta cgaaaacgcc aactttcttc gggaactggc tgaaagcctg
       61 ccccgcatac tgccggaagg tggcccggac aaagctgcgc tgttgcagcg cctggctaac
      121 gaagaactgg cccaggcaga gtatgaagat caggttcgcg ccaaagtgac cgccgcccgt
      181 gctgacacgc gtccgggcat gaccacggaa caactccgcc agcgcctgca tggccgttat
      241 caggaactgc gcgatgccgt atgacgtgga atggaagcag ggagccatcg aagatgtaac
      301 gacactgttt gactatatcg cggaaaattc atcgctgtgg gatgcgcaga acgttaccga
      361 ccgggttctc gctgctgcgg acaaactggc ggacttcccg cgcctctacg aagtggactc
      421 acgttatggc gaagacgttc gccggatcag cctgatggga caacatgtcc tctatgaagt
      481 ggacgacccc gcgcagaaag tccgggttct ggccgtggtc ggccagcgcc agaatcccca
      541 taccgttcgc taataaccgg tgacaggggc gcaattcatt gcgtgcccgt cgccggttgg
      601 acaagcgcag cgcgtcagcg ttttccccaa tgccaccacc ggcgcggcgt ttcggccggt
      661 gtctctacgg tctccggttt gtgttccagc agtctcatgg cctgcatttc ctgccgcaac
      721 ccggccacct cctccctgag tgcttttatc tccgcgatca acgcttcaga ggccgttttt
      781 tcagtctcat ctggcagtct caacccgtcc cgctcgtgag tctcaccctg tcttaactca
      841 ccgtacaccc ggataagctc gctggtatcc acaacccgac cgccatcagc ctccgtccgg
      901 tatgatacgc ggcctttcgc catatcccgg tacagggaac tccgggcttt ccccgtcagt
      961 tccgtcgcct gtctcaccgt cacccacgcc attgtctcac cccgctattt ttgagacagt
     1021 atacccgact ggcgcacacc atgcccggaa gggaaaaaat catgacgaag caggaaaaaa
     1081 ccgccctcaa catggcaaaa ttcatcaggg atcagtcgct gctgttgctg gaaaagctga
     1141 acgagctgga catggacgac ccggccgaca tgtgcgaacg acttcacgag gatgcggaaa
     1201 aactctggct ggtaatgcgg ggtaagctcg gggaagaata acagcaccgg catggccggt
     1261 gctggcgttt attttttgcc gaacggccac caccggcggg cgggtttatc ctccggcaat
     1321 acgggcaccg gctcgttaaa ataccggctg ttatactctc gcatcagctc cgctttcagc
     1381 ttctcgttca ccgcactctc accgtgccgc tcactcacca cgtcaatcat cgcgccgatc
     1441 tgctccgggg tgtacttccc tgcgtccagt acctgcacgc cgttctcgat ggcgaactgc
     1501 tcaaccttgc ggtaaagata atcacacagc ttcattccgg acggtttatc acgttcccac
     1561 gccggatttt tgccggtgaa gtaatacgtg gctttgtcat cgctgttcag ccgttcgtga
     1621 tacatgccga taaacttcat catcgccaga tgctgggcct gatcttcaaa ccggaataca
     1681 tatgccccgg tctgccaggc catgaccgcc atgtcttcca gatgctcgct ggttggcata
     1741 atgcagcgct cgccgtcttt gtcttccaca tcctcaataa aaaccatgta cagcgccata
     1801 atccctcact ctgttgtcat aaatttaaag cacgcgaaca gcctctcccg gtccagttcc
     1861 gccgttttgc gggcttcgcc ggtctgcggg ttcacgattt catccaccgc cagaatcgcc
     1921 cgctgtaccc ggacaagcgt cggccggtcg gggcagatgt accagatttc atcgatgccg
     1981 ttcgccttac ggttgaacag atacccggac accacttcct gataccggcg acggctcttg
     2041 acggtgcgct ccacctcaaa cgccaccgtt ttaccgtcca gctctgccag cgcatccggc
     2101 accttcatac ctttaagact caggcggtgc agggtgcgcc agccggtggc ccctctggct
     2161 tccatatcaa gacgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc
     2221 cgcgagggct gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa cgagaacgcc
     2281 acgccgtgcg gggtcagccc ccacacgctg acctgacgac cgtcaaccgg gtatttcgcc
     2341 cgggccagca gtcctttcgc caccaggctg tccagaaagc ggtaaaccgg ctgcaccgcc
     2401 ccgatcccca gcagacgggc tacggtttca gccgtcgagc agagttcgtc ccgcagccac
     2461 cgcaggacta gccgggtttt ctccgcgttc cgcgcctgtc gcttcgcgtg gctgacgttg
     2521 tattgctcca gagccatcag tacagccccg caaaatcatc gtcacccggg cgctgccgca
     2581 ctgcctcagc ctggtactcc gccagcgggt ccgttttcgc cagcaccggg aaggttttca
     2641 gcggaatttc ccggcgatca acccgcagcg ggctgctgaa cgccagccgc gccacgccca
     2701 gcccggtcat gaccgcgaac ccgtccggca ggtgttgcag ggtgttcacg tcgaacagcg
     2761 gccgcgcttc cttctggatg aagctgtccc cgctgatgtg ctccacgttc cccgcctccc
     2821 ggctggcccg gcggcgctcc acgtccaccc ggatttcccc ggtctgtccg gccgcccact
     2881 gcgcggactc ctggctggcc gcgcgataga accagcggat tggcgtgtta tccagtaccg
     2941 tggttttcac aaaatcagcg ggcagatcct gcccgcactg gccgaagtcg cccagcgact
     3001 ggtgagccag cagcaggtta cagtcccggc tgcgcagcgt ccccagcgcg ttgagtacgt
     3061 acttcgagag cagatatttg atttcatcga gcatcacgct gacgtgcggc cacgcgcgga
     3121 actcgtcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcattttc tgcacccgga
     3181 tcaccgcctc gtcgtccatc gatccgatga cgtacagaca gccgccgccg ttgataatcc
     3241 ccgccacatc aataccggaa tccgtctgca ggacgctcag gtccgcgacg ttctccagct
     3301 tggtgatcag ccccttcacg tcctttttca gcgcctccgg cagcgcatac gccgccgcca
     3361 gcacctgctg aatgttcgcg ccgtgcggga actgctccgc gataagcttc gccgctttct
     3421 gctcgctgat gcggtagtgg tcggccacat cgccggtttc cgccaggtta aacccggcaa
     3481 ccagcagatt tttcagtgcg tactggtcaa ggtcgcggaa caggttcagc tgcgccggtt
     3541 tccccggccg caggtccagc agggtgaagt ccgggcagtg cgcccgaaag acgtgtggcg
     3601 cccatgcgtc gcctttcggg tcgaagtaca ccaccgcatc gccaaaatcg cgcacgcagc
     3661 ggatcagcgc gttggtggcc atcacgctct tgccgctccc gggtaggccg aggatctgaa
     3721 tattggtctt gcgccacgcc tcgcgcggaa cccgcaccgg ctggccgtcc tcgtccttac
     3781 ccaggaacac cacctgctgc tgccgggcct gctccgtgga aaaatcggcc agcgcgttga
     3841 ccggcaccgg ctccccgaaa tgcaggtggc gcacgtcagt catttcgccg gtgcgctggc
     3901 tgctgcgccg gctctcctga ttcagttttg cattgaccgc ctcgtcagcg gccgcaaaat
     3961 gaccgtcaag cagcaggaat gccagtagcc gcgccagaag tatcccgacg accagaccac
     4021 cggcacacca ggctatcccg aacagattag cgggttgcag caggtaataa tcggcattcg
     4081 cgtagtagcc cggacgcaac accgtttctc cccagctctc gataaacacg gcgacaacca
     4141 gcccccccag cagaaccaga ccggcagcgc cggtcagcca gtacgcgtag ttccagacat
     4201 gtcgacgagc cctgaccatc cgcccgggag aatccagcga ggccagcacc gaaaatggcg
     4261 cagccctgtc attatcgcgc ttatagcgat cggcatactc ccgccgctcc ttatggctca
     4321 ggcccacaaa acgcatccgt ccggcccaga gttcagccag accgaaaaac aggccgataa
     4381 ccagaaaagc cccggccaga ccgtaatact cccggtacag ccagctgacc cacgggtaaa
     4441 tctgccgggc cagctccggg atatgccact gccaggcaag cgaatccgta ttcaacatgc
     4501 aacctctctc ctacagacga cggatttacc ggcgtgatgc gcagcccatc tgcgcaagct
     4561 gacgacatgc cccaaggcat ggcggcggcg ttcacgtccg tggtgaaagc gttcagcgct
     4621 cgccacggag gccagcctct ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa
     4681 cgagcacgcc cacagcgtgc gagaggaaaa ggcggcaccc cagtgctgtc ttttccggtg
     4741 ttccggcccc cgtcagagta agcgacagcg agcggctgac cacccataaa atcaggagca
     4801 gctttgctgc ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg tcaggcgtga
     4861 gcctgacggg ggccgggtga atttctggcg tgaccggcgt ccagccggac agagccagaa
     4921 agagggggaa agaccgtgcg tcggtggcgt ccagccagcg taagcagggg atttccccct
     4981 gaagtcagcc acgccccagc ggggatgact tggggaaaga ccgtgcgtcg gtggcgtcca
     5041 gccagcgtaa gcaggggatt tccccctgaa gtcagccacg ccccagcggg gatgacttgc
     5101 atttctccgc aaggagaaaa aagcaacaga gataggttaa cttaaaatgt tcagcgaaaa
     5161 accgacatgg ttcacctatt cggataattg atcgtcaggg aatagaaaga cttaatcagg
     5221 ggacaatatc ccacatcagc gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc
     5281 ctcctcgctc actcggtcgc tacgctccgg gcgtgagact gcggcgggcg ctgcggacac
     5341 atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacgg
     5401 cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa
     5461 cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac
     5521 gggcgaaacc cgacaggact taaagatccc caccgtttcc ggtaggtcgc tccctcttgc
     5581 gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgcctttctc ccttgcggga
     5641 agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct
     5701 ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta
     5761 actgttcact tgagtccaac ccggaaaagc acgcaaaacg ccactggcag cagccattgg
     5821 taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgca
     5881 ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta
     5941 ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt
     6001 tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6061 gatcttttct actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt
     6121 ccaccctgaa catcccggat atagtttgcc tgacatgcga ccagaagaaa gcctaaaaaa
     6181 atcaatataa aaagaaaaac aacctttacc tttttcatat accttccctg tatttaaaaa
     6241 ccccggcatt catccctgaa caccggaagt ctgtatttca aatcatcctt gataattttt
     6301 tgttaccagt taccgtcccg ataatcaaat gaaatttgat accagtactt atcgacttca
     6361 attttatttt taaaatatgg ctgcaacaac ggaacccatg gtttctcaac atcaaacccg
     6421 ccattgttaa tattatcctt taaaggcatt ccaaggcttt caatgacaga accatcatca
     6481 ccaaagtctt tagaatactc ctcaccaata aattcctcag tcttcttatc aaaccaggtt
     6541 aaatttaatt taagccccat acttacctct caaagatatt ttttaatgtt tcgtcctttc
     6601 ggatccggac ctttaatttg cttacccgtt tttggatcaa atgccccgag gtgttcgcca
     6661 tcactggcac gatacccttc aagctcaccg tgctgggagt cccattcata aaccttacgg
     6721 cctttatcac caatccaacg gtcacgtcta ccaccaccac cttgtttagg tgttttttta
     6781 ggtgcttttt ttaattcccc caaccccttg atttcttcgg tcttcggagc gggatgataa
     6841 tcatgaccgt aatctttaac tcccttgcga ggcttgttct tttcctcatt taacttattt
     6901 tctgcatcct tcgctttttt ctccttttgt ttccggctct ccatcgcccc ggtaagtgca
     6961 gccttatctc tggataagga ttcattcatt gcgtttaaag atgcctgagc cgcttttaat
     7021 ttctcattct gattctgaat atccttatca agatagcgaa cctgatcctg ccacccggag
     7081 ccatattcat gaggcgggtt ataagtcaca aaatcatctt tatttgcctc tgcctctttt
     7141 acctttttat cggcttcctg aacagcagcc cttgcaccgg ggagcgcctt ctctgtcgat
     7201 gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg catccagctc agctttcgct
     7261 ttatcatagt ctcttttcag cccctcttcc ggatgagcgg cgtcccatgc ctgctggcga
     7321 cgcttttctt cctcctgacg ctgttttacc tgtgccgggg taagaacatc tgtcaccgac
     7381 acataaaccg gcttctgtcc tgtctctttc gggaaacgaa taacggcctc acgggagtta
     7441 ccaccggctg taaaaccagc cggacgtgaa tcaccttttt cagcaacaat gccttttggc
     7501 ggggctttcg ctgtcggaac acctttcggt acgctcacct gcagagacgg gagacccgga
     7561 atcgacacac tgaatacccc cggacgtttt gtcggtttcg catccaccac agggacactc
     7621 attggccgac ctgcgacaac cgcaatatgc tgccgctcat ccttcaccac atccacaacc
     7681 cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag tacttgccgg agtctccgtc
     7741 accgcatcgg ccggtaatga cgtcataatt tttgacatca ttttcggatc atcttttgca
     7801 atctcagaag gcagcacacc gtagatcgca atcccccaca gaccaaactt aaacggcccc
     7861 ttcagggcag ccagcacatc agcaacggcg gccgacaacg catcgccgga aactgataaa
     7921 gccagtcctt cagcccccgg agtagccagg gcaggcagac cgaaagccat cgcagtggcc
     7981 gaagactgtc cgccaccaga gctaccgcta tgattgccgc tgttaccacc attaccaccg
     8041 ggtttactcc cgccgttacc aaactccccc ggattatagt tatgaatatc tccattcggc
     8101 gtatgcgtcg taccccagcc cggcccgctg tttggatccg taccaccacc tgatgaaggg
     8161 ccacctttac cagacgtacc gttcacattc ccactggcct gaccaccatt atgtcccaga
     8221 cctgaattac ccggacctcg accgtctcca ccactcatat cgttttcctc tttgacattt
     8281 aaaacaataa attaaaacca atatactgta tatatacaca gtgtaattgt taccttactc
     8341 ctccagtttt caggtgtcaa tatatgagat tagttctcat cttcaggcac aaaaaagcgc
     8401 ccggagccgc ctgtaagcag atcgcgtctt ttcccgaaaa tttctcatgg caacaaattt
     8461 tattatttga gatattcatc acacttactt ttatattggt atagatgtac ctgaaagcga
     8521 aatggcatga ggagagtacc tgcggttaaa ctgttcagga atcggagaca acccggaggc
     8581 caccatgacg cacctgaaga aaagcgcctg gcggacgcct ttgtcgctca ggcactggcc
     8641 atgggcctga aaggggttcg aggtccaacc gtacgaaaat gtacggtaag agtgaaattc
     8701 tcgtccgaaa gaaggaaaag caaaactggt aacgccaccg ccggtagaag ggaactatct
     8761 gcaccgcgat tagctgacag atcaagagct ggcggcatcc agaaaacctt aaaaataaag
     8821 ccccttaggg gctttactgg tatcaacttg cagggtgtgc gcctggcggc ggtgtaccgt
     8881 tcgactgggc aacattgcgt aacagcgcca gtatgccgtc ttcaccaaac tccttgtaca
     8941 accggctcat ttccttctga gcattggtgc gcttctggct gttcaggtta cgttcgcact
     9001 ctttcatcat cttttccggc tctgatttca gccttgcaag atcgtcctca tcgatacaga
     9061 aagccgcctg ctttaccgca ccacctacat cagcctttgt cgaaatcgtt ctggccgtaa
     9121 ctggcagata tttttgctta agcgaatggg ccacggctga cgcacggtta acacatagat
     9181 cggccgtgat gttatagccg gaagtggtca ctacgttgcc gtcacagagc gccgcaaaag
     9241 tacacaggct ggttttattt agtttctgga ttaactccag cataacggta ttaccggcga
     9301 agtgattctg gagcttatgg cgagcaattt caggaggata cagatcagtt actttttgca
     9361 tttatagcac ctgtttacag aggataaata acaggtgacg gtgtccggat gagcattcgc
     9421 acatcaaaaa actgtgaaaa atgcctggca agtcggtacc aagttactac ataatgcagc
     9481 accacttgac aaacgcccta atcgggcgtt tgttgatcag gtaagattca gttttcacga
     9541 actgagtcac cggcagctat cagaatcctt agtttctcag gccgaagatc tgatagctgc
     9601 ctcgtttttc actgacaacc tcacgatcat cagaatccct tattctacct tattccataa
     9661 gcgcacacat agcttttttg cagatctttc gcgcttgatt tacaaggaaa ttacctgtct
     9721 ggtcgatgaa attagcaaca gcgctatcag atgacgctaa tgttaaactc atgtttaaac
     9781 atacagcgaa aagttgacgc aaaaatcgtc agctgaatgc ctcataacca gccatttacc
     9841 ataacatccg ttgcccgcac cccctgagag cctctgaggg catttctgac gggaaaccga
     9901 taaaacagac aacaaaccac ccgcaacacc cccggaaacg cgccggataa attgtacctg
     9961 aacgcatggc tatgcacgta actgcatggc atgggatttg acgtaaaatg gatgccgtaa
    10021 gccctttttt accgactttc tgcttcatca gcctgactga cacgagcata ttctttcagg
    10081 ccgcctcctg ctaaactttt tcatattgcc gcattaaccc gggaggctcc gca
//
