LOCUS       NZ_CP154594             1934 bp    DNA     circular CON 09-MAY-2024
DEFINITION  Citrobacter freundii strain NFYY361 plasmid pNFYY361-7, complete
            sequence.
ACCESSION   NZ_CP154594
VERSION     NZ_CP154594.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41129895
            Assembly: GCF_039554995.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Chen,J., Li,X. and Rui,Y.
  TITLE     Citrobacter freundii strain NFYY361
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Chen,J., Li,X. and Rui,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) Laboratory Medicine Center, Nanfang
            Hospital, Southern Medical University, North Guangzhou Avenue,
            Guangzhou 1838th, GuangZhou 510515, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. V0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 323.0x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039554995.1-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 13:35:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,648
            Genes (coding)                    :: 5,491
            CDSs (with protein)               :: 5,491
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 41 of 157
            Pseudo Genes (incomplete)         :: 109 of 157
            Pseudo Genes (internal stop)      :: 29 of 157
            Pseudo Genes (multiple problems)  :: 22 of 157
            Pseudo Genes (short protein)      :: 1 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP154594.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="NFYY361"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="pNFYY361-7"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.19 N 113.33 E"
                     /collection_date="2023"
                     /collected_by="Jing Chen"
     gene            1..303
                     /locus_tag="AAIM57_RS28835"
                     /old_locus_tag="AAIM57_28835"
     CDS             1..303
                     /locus_tag="AAIM57_RS28835"
                     /old_locus_tag="AAIM57_28835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            290..1276
                     /locus_tag="AAIM57_RS28840"
                     /old_locus_tag="AAIM57_28840"
     CDS             290..1276
                     /locus_tag="AAIM57_RS28840"
                     /old_locus_tag="AAIM57_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     Typing          complement(1003..1191)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 atggctggtt cgatccgcgc gcgatccttg tatttatact taagggataa ctctggcgat
       61 aaaaaatggc atcaagcctt gcgtaccaag gcttcggggc ttatttgcgt tgtagaaggt
      121 tttggaagta ttctgttggg agtggtcgcc ttaaaagcga cccccgcaac gacggccatc
      181 gttcgggggt gtgaccacct gcagaaattc gattcgaagc gacaaccaac aggtgatacg
      241 gcaatgataa cacgtcaaat gagccatcgc ccagcatggg acggtgtgca tgcaaaaccc
      301 taaactccag aacctcacgg attactcccc ctcagacgcg ccctgggacg ctcacaaaag
      361 cgtttctgac gacgtaggtg ggatttacct tctcgcagcc gaatatgagc gctacggggc
      421 gcgtatggct tcctgtggcg gtttactgag gtttggttgg tctaccctca aagaaactgg
      481 agaaactcgc cttaggctac gtgaggcgca tttctgtcgt gtccggcact gtcctgtctg
      541 ccagtggagg cgatccctga tgtggcaagc ccgtttttat cagtccctgc cgcgtattgt
      601 tgcggattat ccagatgcac gctggatgtt cctgaccctc acagtgcgaa actgcgcgat
      661 cggggaattg ggtgagacac ttaaccgaat gaacatggcc ttccagcgac ttaaggatcg
      721 caaagagttt cgacctgttc agggctggat ccgcaccacg gaagttacac gtggaagtga
      781 cggtagtgcg cacccgcact ttcacactct gatgatggtt cctccctcaa tgttcacgcg
      841 agattacgtt aaacatgacc gctgggtaga gctttggcga gagtgtttgc gtgtgaatta
      901 cgatccgaat gttgatgttc gagcagtaaa accccgcaag cctaaggacg gagaatcatt
      961 ggcttctgca actgcggaac ttgtacgggg cgcagtggct gaaaccctga aatattccac
     1021 gaagccggct gacatggttg ctgatcctga atggttcctc gaattgacta agcagacaca
     1081 taagcgccga tttgtggcta ccggtggcgc actcaaagac atactgaaac tcgatcagga
     1141 aactgatgcc gatatggtaa tcggggatga tattcccgaa ggcgatgatg atggttcacg
     1201 cattgctttt gagtggaaaa ccgaatccaa aaagtaccgt cgctcaccgt caaaagataa
     1261 agctgaatca gactgacagg gtgtcggggc gcagccctga ccacgacgtt tttgagagga
     1321 cgccgcttgt cggcggtact ccaaaaacac atcttgtcct attggttcta ctccgatgca
     1381 ggatagggct aacgccgcaa agtgacgtca ctcaattact gagttcggtt tccgaactca
     1441 gttgggacaa gatgtgtttt tggtacaccg ccatcacgat gtcggtgcca aaaacgattc
     1501 gtcagggctg cgccccgaca ccctgccaac acctcacgca gtcagattaa aatctgccag
     1561 cgctcgctgt ttccgaaatc gtcaaactct aaatcgtggg gtcccgctgg cttgccagtg
     1621 ggttccgatt tagtgattga cgacattgct gattgaaaaa ttggctgagg tggttggatt
     1681 cacgaagtga ggccaaccac ctggaagacg gtttttaatt gtctggtacc ggatacaacc
     1741 agaacgaatg gagttggttc tgtcgtgatg cgaaaaagta tgggttcgca aatcacagac
     1801 taaatagatt tacccaattc cattcgagtg aggttgttat gttttttgag gttcttaatc
     1861 atgttgatag tgcgttgagc gttgctgttt cgctcctggc gttgtggcag tttttggtgg
     1921 cgggtgggcg ttga
//
