LOCUS       NZ_CP154431            10061 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain Kp459 plasmid p459-3, complete
            sequence.
ACCESSION   NZ_CP154431
VERSION     NZ_CP154431.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41106964
            Assembly: GCF_039515985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Hong,H. and Liu,Y.
  TITLE     cefiderocol resistance in Carbapenem-Resistant Klebsiella pneumoniae
            in China before its approval
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Hong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2024) Department of Clinical Microbiology, First
            Affiliated Hospital of Nanchang University, Nanchang University,
            Nanchang, Yong wai zheng jie No.17, Nanchang 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 288.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039515985.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 08:08:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,588
            CDSs (total)                      :: 5,463
            Genes (coding)                    :: 5,269
            CDSs (with protein)               :: 5,269
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 76 of 194
            Pseudo Genes (incomplete)         :: 128 of 194
            Pseudo Genes (internal stop)      :: 30 of 194
            Pseudo Genes (multiple problems)  :: 31 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP154431.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp459"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p459-3"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2021"
     gene            complement(join(9123..10061,1..1026))
                     /locus_tag="AAIB09_RS27875"
                     /old_locus_tag="AAIB09_27875"
     CDS             complement(join(9123..10061,1..1026))
                     /locus_tag="AAIB09_RS27875"
                     /old_locus_tag="AAIB09_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2510..2659)
                     /locus_tag="AAIB09_RS27880"
                     /old_locus_tag="AAIB09_27880"
     CDS             complement(2510..2659)
                     /locus_tag="AAIB09_RS27880"
                     /old_locus_tag="AAIB09_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2744..3001)
                     /locus_tag="AAIB09_RS27885"
                     /old_locus_tag="AAIB09_27885"
     CDS             complement(2744..3001)
                     /locus_tag="AAIB09_RS27885"
                     /old_locus_tag="AAIB09_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3011..4696)
                     /locus_tag="AAIB09_RS27890"
                     /old_locus_tag="AAIB09_27890"
     CDS             complement(3011..4696)
                     /locus_tag="AAIB09_RS27890"
                     /old_locus_tag="AAIB09_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5023..5268
                     /locus_tag="AAIB09_RS27895"
                     /old_locus_tag="AAIB09_27895"
     CDS             5023..5268
                     /locus_tag="AAIB09_RS27895"
                     /old_locus_tag="AAIB09_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5542..5913
                     /gene="tolA"
                     /locus_tag="AAIB09_RS27900"
                     /old_locus_tag="AAIB09_27900"
     CDS             5542..5913
                     /gene="tolA"
                     /locus_tag="AAIB09_RS27900"
                     /old_locus_tag="AAIB09_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5910..6275
                     /locus_tag="AAIB09_RS27905"
                     /old_locus_tag="AAIB09_27905"
     CDS             5910..6275
                     /locus_tag="AAIB09_RS27905"
                     /old_locus_tag="AAIB09_27905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6450..6728
                     /locus_tag="AAIB09_RS27910"
                     /old_locus_tag="AAIB09_27910"
     CDS             6450..6728
                     /locus_tag="AAIB09_RS27910"
                     /old_locus_tag="AAIB09_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6716..7030
                     /locus_tag="AAIB09_RS27915"
                     /old_locus_tag="AAIB09_27915"
     CDS             6716..7030
                     /locus_tag="AAIB09_RS27915"
                     /old_locus_tag="AAIB09_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7194..7622
                     /locus_tag="AAIB09_RS27920"
                     /old_locus_tag="AAIB09_27920"
     CDS             7194..7622
                     /locus_tag="AAIB09_RS27920"
                     /old_locus_tag="AAIB09_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7648..7827
                     /locus_tag="AAIB09_RS27925"
                     /old_locus_tag="AAIB09_27925"
     CDS             7648..7827
                     /locus_tag="AAIB09_RS27925"
                     /old_locus_tag="AAIB09_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7854..8063)
                     /locus_tag="AAIB09_RS27930"
                     /old_locus_tag="AAIB09_27930"
     CDS             complement(7854..8063)
                     /locus_tag="AAIB09_RS27930"
                     /old_locus_tag="AAIB09_27930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_269707292.1"
                     /translation="MQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSR
                     YFNVPVPELPEDKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8050..8385)
                     /locus_tag="AAIB09_RS27935"
                     /old_locus_tag="AAIB09_27935"
     CDS             complement(8050..8385)
                     /locus_tag="AAIB09_RS27935"
                     /old_locus_tag="AAIB09_27935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_343256634.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     EKRCAGG"
                     /name="hypothetical protein"
     gene            complement(8392..9123)
                     /gene="mobC"
                     /locus_tag="AAIB09_RS27940"
                     /old_locus_tag="AAIB09_27940"
     CDS             complement(8392..9123)
                     /gene="mobC"
                     /locus_tag="AAIB09_RS27940"
                     /old_locus_tag="AAIB09_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9696
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1829..2407
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8396..9123)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cccggcaacc agcagattct tcagggcgta ctggtcgaga tcgcggaaca gattcagctg
       61 cgccggtttc cccggccgca gatccagcag ggtgaagtcc gggcagtgcg cccggaagac
      121 gtgcggcgcc cacgcgtccc ctttggggtc gaagtacacc acagcgtccc cgaaatcgcg
      181 tacgcagcga atgagcgcgt tggtggccat cgcgctcttg ccgctcccgg gcaggccgag
      241 gatctgaata ttggtcttgc gccaggtatc acgagggacc agcaccggct ggccctgctc
      301 atccttaccc agaaacaccg cctgctgttt ccgggcctgc tccgtggaaa aatcggccag
      361 cgcgttaacc ggtaccggct ccccgaaatg caggtggcgg acgtctgtca tttctccggt
      421 ccgctggctg ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc
      481 cgcaaaatga ccatcaagca gcaggaacgc cagcagccgc gccagaagga cgccggcgac
      541 aagccctccg gcacaccagg ctatcccgaa caggttagcc ggctgcagca ggtaataatc
      601 tgcgttcgca tagaggtccg ggcgcaggac ggtttctccc cagctctcga taaacacggc
      661 gacaaccagc ccccccagaa gggccagacc ggcagcgccg gtcagccagt acgcgtagtt
      721 ccagacatgt cgccgtgccc tgaccatccg cccgggtgaa tccagcgagg ccagcaccga
      781 aaatggcaca gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgcgcctt
      841 atggctcagc cccacaaaac gcattcggcc ggcccagagt tcagccagac cgaaaaacag
      901 gccgataacc agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca
      961 cgggtaaatc tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt
     1021 aaacatgcaa tctctctcct tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct
     1081 gcacaagccg cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg
     1141 ttcagctttc accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg
     1201 gaggaacaac gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt
     1261 ttccggtgtt ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat
     1321 caggagcagc tttgctgctt cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc
     1381 aggcgtgagc ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacag
     1441 agccagaaag aggggaaaag accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct
     1501 tttcccctga agtcagccac gccccagcgg ggatgacttg catttctccg caaggagaaa
     1561 aaagcaacag agataggtta acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat
     1621 cctgataatt gatcgtcagg gaatagaaag acttaatcag gggacaatat cccacatcag
     1681 cgcgtccggt gaccgtcttg caggtgccgg ggaaattgcg cctcctcgct cactcggtcg
     1741 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
     1801 ttccgtggat aagcagggga ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg
     1861 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt tttcggtgca
     1921 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
     1981 ttaaagatcc ccaccgtttc cggtaggtcg ctccctcttg cgctctcctg ttccgaccct
     2041 gccgtttacc ggatacctgt ccgccgttct cccttgcggg aagtgtggcg ctttctcata
     2101 gctcacacac tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca
     2161 agaactcccc gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa
     2221 cccgggaaag cacgcaaaac gccactggca gcagccattg gtaactggga gttcgcagag
     2281 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
     2341 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
     2401 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
     2461 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     2521 cgatccccgt cagttcagag gaggacgatg gtgccaccgt tccgccctga acatcccgga
     2581 tataatttgc ctgacatgcg gccagaagaa agccagcaac aatcaatata aaaagaaaaa
     2641 tagtctttac ctttttcata tactttccct gtatttaaag actccggcat tcatccctga
     2701 acaccggaag tcagtatttc aaatcatcct tgataatttt ttgttaccag ttaccgtccc
     2761 gataatcaaa agctatataa caattatcca catcaggttt tatttgattt ttaaaaaatg
     2821 gctgtagtaa aggaacccat tctttcttca aatcaaactc accattatta atattatcct
     2881 ttaaaggcat tcccaagctt tcaatgacag aaccatcgtc accaaagtct ttagaatact
     2941 cttccccttt aaactcttca gttttcttat caaaccatgt caaacgtaat ttaagcccca
     3001 tatttacctc tcaaagatat tttttaatgt tccgttttgg atctggccct ttaacctgct
     3061 tacccgtttt gggatcaaat gcgcctaggt gttcgccatc actggcacga tacccttcaa
     3121 gctcaccgtg ctgggaatcc cattcataaa ttttacgttt tttatcgcca taccatcggg
     3181 cccgcttacc accaccacct tgtttaggtg ttttaggatc acctcgcttt aactctccaa
     3241 gtccctttat gtcttctgtt ttaggtactg gatgataatc atggccgtag tctttagtcc
     3301 ccttgcgagg cttgttcttt tcctcattta acttattttc tgcatccttc gcttttttct
     3361 ccttttgttt ccggctctcc atcgccccgg taagtgcagc cttatctctg gataaggatt
     3421 cattcattgc gtttaaagat gcctgagccg cttttaattt ctcattctga ttctgaatat
     3481 ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga ggcgggttat
     3541 aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg gcttcctgaa
     3601 cagcagccct tgcaccgggg agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg
     3661 caatattttt atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc
     3721 cctcttccgg atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct
     3781 gttttacctg tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg
     3841 tctctttcgg gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg
     3901 gacgtgaatc acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac
     3961 ctttcggtac gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg
     4021 gacgttttgt cggtttcgca tccaccacag ggacactcat tggccgacct gcgacaaccg
     4081 caatatgctg ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg
     4141 cctggtccag tggtaaagta cttgccggag tctccgtcac cgcatcggcc ggtaatgacg
     4201 tcataatttt tgacatcatt ttcggatcat cttttgcaat ctcagaaggc agcacaccgt
     4261 agatcgcaat cccccacaga ccaaacttaa acggcccctt cagggcagcc agcacatcag
     4321 caacggcggc cgacaacgca tcgccggaaa ctgataaagc cagtccttca gcccccggag
     4381 tagccagggc aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc
     4441 taccgctatg attgccgctg ttaccaccat taccaccggg tttactcccg ccgttaccaa
     4501 actcccccgg attatagtta tgaatatctc cattcggcgt atgcgtcgta ccccagcccg
     4561 gcccgctgtt tggatccgta ccaccacctg atgaagggcc acctttacca gacgtaccgt
     4621 tcacattccc actggcctga ccaccattat gtcccagacc tgaattaccc ggacctcgac
     4681 cgtctccacc actcataccg ttttcctctt tgacatttaa aacaataaat taaagccaac
     4741 atactgtaca tatacacagt ataattatta ccttactcct cccgttttca ggtgtcaata
     4801 tatgagatta gttctcatct tcaggcacaa aaaagcgccc ggagccacct gtaagcagat
     4861 cgcgtctttt cccgaaaatt tctcatggca acaaatttta ttatttaaga cattcatcac
     4921 acttacattt cagatggtat agacacatct gaaagctaac tggcatgagg agaggacctg
     4981 cggttaaact gttcgggaaa cctgagacgc ccggaggcca ctatgacgca cctgaaactg
     5041 actcccgctg aagaaaagcg cctggcggac gcctttgtcg ctcaggcact ggccatgggc
     5101 ctgaaaggga acaaaacgct gccaaaggcg gtaaaacaac aggtgcggga aagggtgctt
     5161 cgtgccattc aggacgaacg tgaagccagg cgggcgcagg aaatgcgtcg ggagaaatac
     5221 gaggctacgg aaaccacctt cacctggcag ccacagcgcc gccggtaagg ggtatttacc
     5281 ataacgtcgc attgcccgca ccgctcagac ggtctgaggg cgtttccggc ggctacggga
     5341 taaaacaggc aacaaaccac ccggaaaacc gccggaaacg cgcccggtaa attgtaaccc
     5401 gctgcatgcc tatgcacgca actgcatggc gggccgtttg gtgtaaaaac gatacgataa
     5461 gccgtgttta tgtaaatgaa cgtggccgta aagagtgaca aatatacatg taacattttt
     5521 tactgacagg tcatcaaccc gatgcgttta atacccttta tcctgctggc attgtgcagc
     5581 cccttactcc tgacggcctg taagcattcc accacccagc cgccgaaaat ttatactctg
     5641 gataagtcat ctaaaatcag tagctacgct gcggccatca agaccgctgt tgaaatgcag
     5701 atgtttgaac cagaggccta tgcgggaaaa atgtgcaccg tgcggatatc catacaacgt
     5761 gatggcactc tgaacagcgc cattgctgaa ggaggagatc cgaaactctg taaagcagca
     5821 atatcagccg ttacccacgc caaaattccg ccggcaccgg atgaaaatac ctggcaaata
     5881 ttcaaaaacg cccctctgga tttcagacca tgaaagcttt tactctgaca gtgctggcgg
     5941 ccatgttcct cactccggta catgcgatac cactaaaacc cgtaaccaca ctgcagccaa
     6001 cctacccggt acaggccgca gcaaaacgca tcgaaggtca ggtgaccgcc acgtttgata
     6061 ttctggacga tggacgagtg gacaacgtgt cgcttaccgg ctgtcacctt ttttttcggg
     6121 aaacgctgac ggcaatgagg cactggctat atcccgcagg gcacccggca atgcgacagg
     6181 ttataaattt tgaattcagt ctgaaaaatg acggcttaca ggatccgcga ccagtcacca
     6241 cccaaaagct caatgatgat ggttgccgcc agtaacaggt cctttaaccc gcaggctgca
     6301 atagtcctgg atgatgagtg agcctgtagc agccctgatt agtggaagcg aagctgaaaa
     6361 atgggcagca aaagccgtat ttctggcaaa tagcgccgcc acggattaaa gtagtcatgt
     6421 gtaatacata tgactacaaa ggggctttta tgtcatcagc aacatccatc aggctcgatg
     6481 aagaacttaa ggaccggctg aaaacgctgg ccgatgaccg gcaccgctca gcgcacgcgc
     6541 taatgctgga ggcaatcacc gaatacatcg accgggaaga gaagcgcagc cagtacctgc
     6601 gggacggaca ggccgcatgg cagcactatc aggaaacggg tctgcacctt actgcggaag
     6661 aagcggaagc ctggataagt acctggggaa ctgagaacga gcaggatgct ccgccatgcc
     6721 acaggtaacg atttccgcgc tggcacagcg cgatttacaa cgccttcagg actttctcaa
     6781 aaccaaaaat cggctggcag ccagaaaagg aggtgaggtg atcgtccggg ctatccagca
     6841 actgaaaaca ttgccagaca ttggccgccc ggtgccgttt ctgccgctgg aatataagga
     6901 actggtgatc gggtttggcg acagtggcta tgtgatgctc taccgccacg accgggaaat
     6961 ggaccagatt gtgatcgtca ccgtcaggca tcagaaagaa tccgggtatc cgggggcaga
     7021 cagcctctaa taaccgatga cgggggcgca attcattgcg tggccgtcgc cggtttgaca
     7081 agcgcagcgc gtcagaaaac cacatcctga acggaaaaac gggtaaaatt cagcgataca
     7141 tttaccgtga gaaattacac acctttcaca cccgataaaa tcaggggtat acagtggcga
     7201 aaacattgct caacctttca caggcagcac aagccgcggg cattaccaga agaacgctat
     7261 acaatcatgt aaaacaggga aaggttaccg tgtcacgtga cgggaaaaac aaccctgtcg
     7321 tggatgtgtc agaactgatc cgtgtatacg gaaatgtgaa tatccctgag aaacaaattc
     7381 ccggcatttc acaccgtgaa aatacacaaa aaaatttccc acaggaacag cttctggcaa
     7441 tgcaaaagga actggccgat ctccggcagg ctgtcaccct gatgctggaa gataaaacat
     7501 cccgggaaga agaacgccgc cagcacgatg atgagcgccg gaaactccag gccgaagtgg
     7561 acagattgac aacagaactg acacagaaga aaaaacgctt ctggtccggc tggtttagct
     7621 gagaacttac cgaagggaat ggagaacatg acgaagcagg aaaaaactgc cctcaatatg
     7681 gctaaattca ttaaggatca gtcgctgctg ttgctggaaa agctgaacga gctggacatg
     7741 gataccccgg ccgacatgtg cgaacggctt cacgaggatg cggaaacact ctggcaggtg
     7801 atgcgggata agctcgggga agaataacag caccggccat gccggtgctg gcgttatttt
     7861 ttgccgaacg gccaccaccg gcgggcgggt ttgtcctccg gcagttcagg caccggcacg
     7921 ttaaaatagc ggctgttata ttcccgcatc agctccgctt tcagcttctc gttcaccgcg
     7981 ctttccccgt gccgctcgct gaccacctca atcatcgcgt taatctgctc cggtgagcac
     8041 ttcccggcat caaccacctg cacaccgttt ctcgatggcg aaccgctcca cctttcgata
     8101 gaggtaatca cacagcttca ttcctgtggg tttgtcccgt tcccattgcg gattttcacc
     8161 ggtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga
     8221 tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg
     8281 ccaggcgagc accaccatgt cttccagatg ctcgctggtt ggcataatgc agcgctcgcc
     8341 gtctttgtct tccacgtcct caataaatac catgtacagc gccataatcc ctcactccgt
     8401 tgtcataaat ttaaagcacg cgaacagcct ctcccggtcc agctccaccg ttttgcgggc
     8461 ctcgccggtc tgtggattca cgatttcatc caccgccaga atcgcccgct gtacccggac
     8521 aagcgtcggc cggtcagggc agatgtacca gatttcatcg atgccgttcg cccggcggtt
     8581 gaacagatac ccggacacca cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac
     8641 ctcaaatgcc accgttctgc cgtccagctc cgccagcgcg tccggcactt tcatcccttt
     8701 cagggccata cggtgcaggt accgccagcc ggtggccccc cggccctcca tggagaggcg
     8761 cagggactgc acggccagcc ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa
     8821 cggaataacg tccgtcagcg gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt
     8881 cagcccccac acgctgacct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc
     8941 tttcgccacc agactgtcca ggaagcggta aaccggctgt accgccgcaa tccccagccg
     9001 tcgcgcaacc agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg
     9061 tgttttctcc gcgttccgcg cctgccgctt cgcgtggctg acgttgtact gctccagtgt
     9121 catcagtaaa gccccgcaaa atcatcatcc gcttttggtc cgccgccgtc ggccggaggc
     9181 aggtactccg ccagcgggtc cgttttcgcc agcaccggga aggttttcag cgaaatttcc
     9241 cggcgctcaa cccgcagcgg gctgctgaac gccagccgcg ccacgcccag cccggtcatc
     9301 accgcgaacc cgtcaggcag gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc
     9361 ttctggatga agctgtcgcc gctgatgtgc tccacgttcc ccgcctcccg gctggcccgg
     9421 cggcgctcca cgtccacccg gatctccccg gtctggcccg ccgcccactg cgcggactcc
     9481 tggctggccg cgcgatagaa ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca
     9541 aaatcagcgg gcagatcctg cccgcactga ccgaagtcgc ccagcgactg gtgcgcgagc
     9601 agcaggttgc agtcccggct gcgcagcgtc cccagcgcgt tcagcacgta cttcgacagc
     9661 agatatttaa tctcatcgag catgatgctc gcgtgcggcc acctccggaa ctcatcccgc
     9721 gcaatgatga tttgcgcgca gcgggcaaac agcatcttct gcacccggat caccgcctca
     9781 tcgtccatcg agccgatgac gtacaggcag ccgccgccgt tgattatccc cgccacatcg
     9841 ataccgctgt ccgtctgcag gacgctcagg tcggccacgt tctccagctt ggtgatcagc
     9901 cccttcacgt cctttttcag ggcctccggc aacgcgtacg ccgccgccag cacctgctga
     9961 atgtttgccc cctgcgggaa ctgctccgca atcagcttcg ccgctttctg ctcgctgatg
    10021 cggtagtggt ccgccacgtc gccggtctca ctcaggttaa a
//
