LOCUS       NZ_CP154348            11970 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain JXHV-KP2 plasmid p2-5, complete
            sequence.
ACCESSION   NZ_CP154348
VERSION     NZ_CP154348.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41075352
            Assembly: GCF_039545785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Qiu,J. and Liu,Y.
  TITLE     The UvrD helicase regulated the copy number of the virulence plasmid
            and contributed to the heterogeneity of ST11-CR-HvKP virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Qiu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2024) Department of Clinica Laboratory,Medical
            Center of Burn Plastic and Wound Repair, The First Affiliated
            Hospital of Nanchang University,Nanchang University, Yong wai zheng
            jie No.17, Nanchang 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29.0x
            Sequencing Technology             :: PacBio Sequel2 hifi
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039545785.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 14:16:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,996
            CDSs (total)                      :: 5,874
            Genes (coding)                    :: 5,705
            CDSs (with protein)               :: 5,705
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 66 of 169
            Pseudo Genes (incomplete)         :: 113 of 169
            Pseudo Genes (internal stop)      :: 22 of 169
            Pseudo Genes (multiple problems)  :: 32 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP154348.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JXHV-KP2"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2-5"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2020"
     gene            complement(59..1546)
                     /gene="ltrA"
                     /locus_tag="AAHF09_RS29915"
                     /old_locus_tag="AAHF09_29915"
     CDS             complement(59..1546)
                     /gene="ltrA"
                     /locus_tag="AAHF09_RS29915"
                     /old_locus_tag="AAHF09_29915"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2191..2436
                     /locus_tag="AAHF09_RS29920"
                     /old_locus_tag="AAHF09_29920"
     CDS             2191..2436
                     /locus_tag="AAHF09_RS29920"
                     /old_locus_tag="AAHF09_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2710..3081
                     /gene="tolA"
                     /locus_tag="AAHF09_RS29925"
                     /old_locus_tag="AAHF09_29925"
     CDS             2710..3081
                     /gene="tolA"
                     /locus_tag="AAHF09_RS29925"
                     /old_locus_tag="AAHF09_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3078..3443
                     /locus_tag="AAHF09_RS29930"
                     /old_locus_tag="AAHF09_29930"
     CDS             3078..3443
                     /locus_tag="AAHF09_RS29930"
                     /old_locus_tag="AAHF09_29930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3618..3896
                     /locus_tag="AAHF09_RS29935"
                     /old_locus_tag="AAHF09_29935"
     CDS             3618..3896
                     /locus_tag="AAHF09_RS29935"
                     /old_locus_tag="AAHF09_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            3884..4198
                     /locus_tag="AAHF09_RS29940"
                     /old_locus_tag="AAHF09_29940"
     CDS             3884..4198
                     /locus_tag="AAHF09_RS29940"
                     /old_locus_tag="AAHF09_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4362..4790
                     /locus_tag="AAHF09_RS29945"
                     /old_locus_tag="AAHF09_29945"
     CDS             4362..4790
                     /locus_tag="AAHF09_RS29945"
                     /old_locus_tag="AAHF09_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4816..4995
                     /locus_tag="AAHF09_RS29950"
                     /old_locus_tag="AAHF09_29950"
     CDS             4816..4995
                     /locus_tag="AAHF09_RS29950"
                     /old_locus_tag="AAHF09_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5022..5552)
                     /locus_tag="AAHF09_RS29955"
                     /old_locus_tag="AAHF09_29955"
     CDS             complement(5022..5552)
                     /locus_tag="AAHF09_RS29955"
                     /old_locus_tag="AAHF09_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5559..6290)
                     /gene="mobC"
                     /locus_tag="AAHF09_RS29960"
                     /old_locus_tag="AAHF09_29960"
     CDS             complement(5559..6290)
                     /gene="mobC"
                     /locus_tag="AAHF09_RS29960"
                     /old_locus_tag="AAHF09_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6290..8254)
                     /locus_tag="AAHF09_RS29965"
                     /old_locus_tag="AAHF09_29965"
     CDS             complement(6290..8254)
                     /locus_tag="AAHF09_RS29965"
                     /old_locus_tag="AAHF09_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9738..9887)
                     /locus_tag="AAHF09_RS29970"
                     /old_locus_tag="AAHF09_29970"
     CDS             complement(9738..9887)
                     /locus_tag="AAHF09_RS29970"
                     /old_locus_tag="AAHF09_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(9972..10229)
                     /locus_tag="AAHF09_RS29975"
                     /old_locus_tag="AAHF09_29975"
     CDS             complement(9972..10229)
                     /locus_tag="AAHF09_RS29975"
                     /old_locus_tag="AAHF09_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(10239..11924)
                     /locus_tag="AAHF09_RS29980"
                     /old_locus_tag="AAHF09_29980"
     CDS             complement(10239..11924)
                     /locus_tag="AAHF09_RS29980"
                     /old_locus_tag="AAHF09_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             5239..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9057..9635
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5563..6290)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gtcgcggtag ggatacccgt taccggatac cccccgcaca gatcccggcg tgcgcgattt
       61 acgcaccggg ctcctgcctc gggtgtctgg cggtgaaccg ctccacaggc catggatgaa
      121 gaacccgaac ccttggtagc catgcggctg ccagtttgtt tgctttcgtc caggtcgtat
      181 catccttctg gctcctgcgc ctgagcgccc ggcgccagag gtttgttacg tgtgtcctga
      241 acttctgcat ggtggggaag ttgcccggta ccgagtgata gttcaggtat ccctgaacca
      301 ctctcctgag ccattttccc tgttcgggga ttgagtaatg ccagcgcctt cgcagaccgt
      361 ctttgatggc tttcagagtt gccgtcatcc gatcccggcg ggtctttcgt atcagcatga
      421 acctgccgtt gcgatctttc ccgctgatgt gcgtgaaccc gaggaagttg aacgtttctg
      481 gtttgccttt tcccctgatg gcacggtttt cggcagcgaa gcggccgaac tccatcagac
      541 gggttttctc cgggtgaacc gtgagtccga actccctcag tctgcgctgc atggctatac
      601 ggaagcgccg ggcatcgtat cgtttgtcga acccgatgac gatgtcatcg gcgtatctga
      661 ccattaccac attgcctgtg gcatagcgac gtcgccactg atgcgcccac agatcgaaga
      721 cgtagtggag gtatatgttt gccagcagcg gtgagatgac cgcaccctgt ggggtgcctt
      781 cctccgttgc tcgccattga ccctcctccg acgtcccggc tgtgagccac ttacgtatga
      841 gcctgattac cctccggtcg ccgatccgat gctctgtgaa cctgatcagc cattcgtggc
      901 tcaccctgtc gaagaactga ctgatgtcgg catccagtac ccagtttacg ttagtgcgta
      961 ccagccctgt ggccagtgcg tccagtgcat cgtgctggct tcgcccgggt ctgaacccgt
     1021 atgagaaccc cataaagtcg ttttcataga ctgcgttcag gattttcacc agcgcatact
     1081 ggacgatctt gtcctccagc gaggcgatgc cgagcgggcg ttgttttcca tccgcttttg
     1141 ggatgtagtg acgcctgccg ggctgcgccc tgtagctgcc ctgatgtagc ctccggtgca
     1201 gatctgttat gttgttcttc atgtttccgg cgtagtccat ccacctgatg ccatccactc
     1261 cggcggccgc tttcctgctc agggagagga atgcggcttc cagtgcttcg actgtcagca
     1321 ggtggaacaa tgctgtaaac cgttctttct tccgctgctt cgcagcttcc cgcacgcgtg
     1381 acagcctctg tgacatgctt tcccggctct gtgtccggcg catgtgtggc tgttccgcgt
     1441 tccccttggc cccgctcctt cgctccactg actccgctcc tttcgggttg ttcgcctgct
     1501 tcgccgctac tatgagcgag tccgacttct cctctccgta catcaccggc tatgactcct
     1561 cgtcttcccg gtgcgggcca tctccgacac tggcagatgg tcagagggga gatctcccgg
     1621 ttcccgcgta gagatcgtat tgacatgcca gggtctcaga ccccgccggg tccatgtggc
     1681 actcgcagtg tcgcacccta tgatgttgcc ttccgttaac agtacaacgt cggcacccgg
     1741 taatttaata tacatttcgt ggctcaatgg ctggcctgtc aacacccctg tcaacgcttc
     1801 gccccatacc tcgcggtatg caacgcatga ctcggggacc ttgtggattg ctggtccttc
     1861 aatggtcggg gactttcacc ccttgatctc taaccggtct cccggcgcac actgtacata
     1921 tacacagtat aattattacc ttactcctcc cgttttcagg tgtcaatata tgagattagt
     1981 tctcatcttc aggcacaaaa aagcgcccgg agccacctgt aagcagatcg cgtcttttcc
     2041 cgaaaatttc tcatggcaac aaattttatt atttaagaca ttcatcacac ttacatttca
     2101 gatggtatag acacatctga aagctaactg gcatgaggag aggacctgcg gttaaactgt
     2161 tcgggaaacc tgagacgccc ggaggccact atgacgcacc tgaaactgac tcccgctgaa
     2221 gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg ccatgggcct gaaagggaac
     2281 aaaacgctgc caaaggcggt aaaacaacag gtgcgggaaa gggtgcttcg tgccattcag
     2341 gacgaacgtg aagccaggcg ggcgcaggaa atgcgtcggg agaaatacga ggctacggaa
     2401 accaccttca cctggcagcc acagcgccgc cggtaagggg tatttaccat aacgtcgcat
     2461 tgcccgcacc gctcagacgg tctgagggcg tttccggcgg ctacgggata aaacaggcaa
     2521 caaaccaccc ggaaaaccgc cggaaacgcg cccggtaaat tgtaacccgc tgcatgccta
     2581 tgcacgcaac tgcatggcgg gccgtttggt gtaaaaacga tacgataagc cgtgtttatg
     2641 taaatgaacg tggccgtaaa gagtgacaaa tatacatgta acatttttta ctgacaggtc
     2701 atcaacccga tgcgtttaat accctttatc ctgctggcat tgtgcagccc cttactcctg
     2761 acggcctgta agcattccac cacccagccg ccgaaaattt atactctgga taagtcatct
     2821 aaaatcagta gctacgctgc ggccatcaag accgctgttg aaatgcagat gtttgaacca
     2881 gaggcctatg cgggaaaaat gtgcaccgtg cggatatcca tacaacgtga tggcactctg
     2941 aacagcgcca ttgctgaagg aggagatccg aaactctgta aagcagcaat atcagccgtt
     3001 acccacgcca aaattccgcc ggcaccggat gaaaatacct ggcaaatatt caaaaacgcc
     3061 cctctggatt tcagaccatg aaagctttta ctctgacagt gctggcggcc atgttcctca
     3121 ctccggtaca tgcgatacca ctaaaacccg taaccacact gcagccaacc tacccggtac
     3181 aggccgcagc aaaacgcatc gaaggtcagg tgaccgccac gtttgatatt ctggacgatg
     3241 gacgagtgga caacgtgtcg cttaccggct gtcacctttt ttttcgggaa acgctgacgg
     3301 caatgaggca ctggctatat cccgcagggc acccggcaat gcgacaggtt ataaattttg
     3361 aattcagtct gaaaaatgac ggcttacagg atccgcgacc agtcaccacc caaaagctca
     3421 atgatgatgg ttgccgccag taacaggtcc tttaacccgc aggctgcaat agtcctggat
     3481 gatgagtgag cctgtagcag ccctgattag tggaagcgaa gctgaaaaat gggcagcaaa
     3541 agccgtattt ctggcaaata gcgccgccac ggattaaagt agtcatgtgt aatacatatg
     3601 actacaaagg ggcttttatg tcatcagcaa catccatcag gctcgatgaa gaacttaagg
     3661 accggctgaa aacgctggcc gatgaccggc accgctcagc gcacgcgcta atgctggagg
     3721 caatcaccga atacatcgac cgggaagaga agcgcagcca gtacctgcgg gacggacagg
     3781 ccgcatggca gcactatcag gaaacgggtc tgcaccttac tgcggaagaa gcggaagcct
     3841 ggataagtac ctggggaact gagaacgagc aggatgctcc gccatgccac aggtaacgat
     3901 ttccgcgctg gcacagcgcg atttacaacg ccttcaggac tttctcaaaa ccaaaaatcg
     3961 gctggcagcc agaaaaggag gtgaggtgat cgtccgggct atccagcaac tgaaaacatt
     4021 gccagacatt ggccgcccgg tgccgtttct gccgctggaa tataaggaac tggtgatcgg
     4081 gtttggcgac agtggctatg tgatgctcta ccgccacgac cgggaaatgg accagattgt
     4141 gatcgtcacc gtcaggcatc agaaagaatc cgggtatccg ggggcagaca gcctctaata
     4201 accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg gtttgacaag cgcagcgcgt
     4261 cagaaaacca catcctgaac ggaaaaacgg gtaaaattca gcgatacatt taccgtgaga
     4321 aattacacac ctttcacacc cgataaaatc aggggtatac agtggcgaaa acattgctca
     4381 acctttcaca ggcagcacaa gccgcgggca ttaccagaag aacgctatac aatcatgtaa
     4441 aacagggaaa ggttaccgtg tcacgtgacg ggaaaaacaa ccctgtcgtg gatgtgtcag
     4501 aactgatccg tgtatacgga aatgtgaata tccctgagaa acaaattccc ggcatttcac
     4561 accgtgaaaa tacacaaaaa aatttcccac aggaacagct tctggcaatg caaaaggaac
     4621 tggccgatct ccggcaggct gtcaccctga tgctggaaga taaaacatcc cgggaagaag
     4681 aacgccgcca gcacgatgat gagcgccgga aactccaggc cgaagtggac agattgacaa
     4741 cagaactgac acagaagaaa aaacgcttct ggtccggctg gtttagctga gaacttaccg
     4801 aagggaatgg agaacatgac gaagcaggaa aaaactgccc tcaatatggc taaattcatt
     4861 aaggatcagt cgctgctgtt gctggaaaag ctgaacgagc tggacatgga taccccggcc
     4921 gacatgtgcg aacggcttca cgaggatgcg gaaacactct ggcaggtgat gcgggataag
     4981 ctcggggaag aataacagca ccggccatgc cggtgctggc gttatttttt gccgaacggc
     5041 caccaccggc gggcgggttt gtcctccggc agttcaggca ccggcacgtt aaaatagcgg
     5101 ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc
     5161 cgctcgctga ccacctcaat catcgcgtta atctgctccg gtgagcactt cccggcatca
     5221 accacctgca caccgttctc gatggcgaac cgctccacct ttcgatagag gtaatcacac
     5281 agcttcattc ctgtgggttt gtcccgttcc cattgcggat tttcaccggt gaaaaagtac
     5341 gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc
     5401 agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc
     5461 accatgtctt ccagatgctc gctggttggc ataatgcagc gctcgccgtc tttgtcttcc
     5521 acgtcctcaa taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta
     5581 aagcacgcga acagcctctc ccggtccagc tccaccgttt tgcgggcctc gccggtctgt
     5641 ggattcacga tttcatccac cgccagaatc gcccgctgta cccggacaag cgtcggccgg
     5701 tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa cagatacccg
     5761 gacaccactt cctgataccg cctgcggctc ttgacggtgc gctctacctc aaatgccacc
     5821 gttctgccgt ccagctccgc cagcgcgtcc ggcactttca tccctttcag ggccatacgg
     5881 tgcaggtacc gccagccggt ggccccccgg ccctccatgg agaggcgcag ggactgcacg
     5941 gccagccggt gcggcagctg cgccgccgac acccttgacg gctgaaacgg aataacgtcc
     6001 gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag cccccacacg
     6061 ctgacctgac ggccgtcaac cgggtatttc gcccggacca gcagcccttt cgccaccaga
     6121 ctgtccagga agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg cgcaaccagc
     6181 tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt tttctccgcg
     6241 ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat cagtaaagcc
     6301 ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc cggaggcagg tactccgcca
     6361 gcgggtccgt tttcgccagc accgggaagg ttttcagcga aatttcccgg cgctcaaccc
     6421 gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc gcgaacccgt
     6481 caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc
     6541 tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg cgctccacgt
     6601 ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg ctggccgcgc
     6661 gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa tcagcgggca
     6721 gatcctgccc gcactgaccg aagtcgccca gcgactggtg cgcgagcagc aggttgcagt
     6781 cccggctgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcaga tatttaatct
     6841 catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca atgatgattt
     6901 gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg tccatcgagc
     6961 cgatgacgta caggcagccg ccgccgttga ttatccccgc cacatcgata ccgctgtccg
     7021 tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc ttcacgtcct
     7081 ttttcagggc ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg tttgccccct
     7141 gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg
     7201 ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc agggcgtact
     7261 ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg
     7321 tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct ttggggtcga
     7381 agtacaccac agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatcg
     7441 cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc caggtatcac
     7501 gagggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc tgctgtttcc
     7561 gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc ccgaaatgca
     7621 ggtggcggac gtctgtcatt tctccggtcc gctggctgct gcgccggctc tcctgattca
     7681 gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc atcaagcagc aggaacgcca
     7741 gcagccgcgc cagaaggacg ccggcgacaa gccctccggc acaccaggct atcccgaaca
     7801 ggttagccgg ctgcagcagg taataatctg cgttcgcata gaggtccggg cgcaggacgg
     7861 tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg
     7921 cagcgccggt cagccagtac gcgtagttcc agacatgtcg ccgtgccctg accatccgcc
     7981 cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt ccggttatta tcgcgcttat
     8041 agcgatcggc atactcccgc cgcgccttat ggctcagccc cacaaaacgc attcggccgg
     8101 cccagagttc agccagaccg aaaaacaggc cgataaccag aaaaaaaccg gccagactgt
     8161 aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc tccgggatat
     8221 gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc agacgacggg
     8281 tttaccggcg tgatgcgcag cccccgctgc acaagccgcg gacatgcccc aaggcatggc
     8341 ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc agcctctttc
     8401 cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca gcgtgtgaga
     8461 ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc
     8521 atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg tcaggtgcgt
     8581 ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc gggtgaattt
     8641 ctggcgtggc cggcgtccag ccggacagag ccagaaagag gggaaaagac cgtgcgtcgg
     8701 tggcgtccag ccagcgtaag caaggtcttt tcccctgaag tcagccacgc cccagcgggg
     8761 atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac gtaaaatgtt
     8821 cagcgaaaaa ccgacatggt tcacctatcc tgataattga tcgtcaggga atagaaagac
     8881 ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca ggtgccgggg
     8941 aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc
     9001 tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag
     9061 gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt
     9121 tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca accatgaatc
     9181 tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg gtaggtcgct
     9241 ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc gccgttctcc
     9301 cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc tcggtgtagg
     9361 tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac tgctgcgcct
     9421 tatccggtaa ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc cactggcagc
     9481 agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg
     9541 aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa
     9601 agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc
     9661 ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga
     9721 agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt
     9781 gccaccgttc cgccctgaac atcccggata taatttgcct gacatgcggc cagaagaaag
     9841 ccagcaacaa tcaatataaa aagaaaaata gtctttacct ttttcatata ctttccctgt
     9901 atttaaagac tccggcattc atccctgaac accggaagtc agtatttcaa atcatccttg
     9961 ataatttttt gttaccagtt accgtcccga taatcaaaag ctatataaca attatccaca
    10021 tcaggtttta tttgattttt aaaaaatggc tgtagtaaag gaacccattc tttcttcaaa
    10081 tcaaactcac cattattaat attatccttt aaaggcattc ccaagctttc aatgacagaa
    10141 ccatcgtcac caaagtcttt agaatactct tcccctttaa actcttcagt tttcttatca
    10201 aaccatgtca aacgtaattt aagccccata tttacctctc aaagatattt tttaatgttc
    10261 cgttttggat ctggcccttt aacctgctta cccgttttgg gatcaaatgc gcctaggtgt
    10321 tcgccatcac tggcacgata cccttcaagc tcaccgtgct gggaatccca ttcataaatt
    10381 ttacgttttt tatcgccata ccatcgggcc cgcttaccac caccaccttg tttaggtgtt
    10441 ttaggatcac ctcgctttaa ctctccaagt ccctttatgt cttctgtttt aggtactgga
    10501 tgataatcat ggccgtagtc tttagtcccc ttgcgaggct tgttcttttc ctcatttaac
    10561 ttattttctg catccttcgc ttttttctcc ttttgtttcc ggctctccat cgccccggta
    10621 agtgcagcct tatctctgga taaggattca ttcattgcgt ttaaagatgc ctgagccgct
    10681 tttaatttct cattctgatt ctgaatatcc ttatcaagat agcgaacctg atcctgccac
    10741 ccggagccat attcatgagg cgggttataa gtcacaaaat catctttatt tgcctctgcc
    10801 tcttttacct ttttatcggc ttcctgaaca gcagcccttg caccggggag cgccttctct
    10861 gtcgatgcaa tgcggctgtt taaggtcgca atatttttat cttcggcatc cagctcagct
    10921 ttcgctttat catagtctct tttcagcccc tcttccggat gagcggcgtc ccatgcctgc
    10981 tggcgacgct tttcttcctc ctgacgctgt tttacctgtg ccggggtaag aacatctgtc
    11041 accgacacat aaaccggctt ctgtccggtc tctttcggga aacgaataac ggcctcacgg
    11101 gagttaccac cggctgtaaa accagccgga cgtgaatcac ctttttcagc aacaatgcct
    11161 tttggcgggg ctttcgctgt cggaacacct ttcggtacgc tcacctgcag agacgggaga
    11221 cccggaatcg acacactgaa tacccccgga cgttttgtcg gtttcgcatc caccacaggg
    11281 acactcattg gccgacctgc gacaaccgca atatgctgcc gctcatcctt caccacatcc
    11341 acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc
    11401 tccgtcaccg catcggccgg taatgacgtc ataatttttg acatcatttt cggatcatct
    11461 tttgcaatct cagaaggcag cacaccgtag atcgcaatcc cccacagacc aaacttaaac
    11521 ggccccttca gggcagccag cacatcagca acggcggccg acaacgcatc gccggaaact
    11581 gataaagcca gtccttcagc ccccggagta gccagggcag gcagaccgaa ggccatcgca
    11641 gtggccgaag actgtccgcc accagagcta ccgctatgat tgccgctgtt accaccatta
    11701 ccaccgggtt tactcccgcc gttaccaaac tcccccggat tatagttatg aatatctcca
    11761 ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc accacctgat
    11821 gaagggccac ctttaccaga cgtaccgttc acattcccac tggcctgacc accattatgt
    11881 cccagacctg aattacccgg acctcgaccg tctccaccac tcataccgtt ttcctctttg
    11941 acatttaaaa caataaatta aagccaacat
//
