LOCUS       NZ_CP154302            11970 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP156 plasmid pKP156-4, complete
            sequence.
ACCESSION   NZ_CP154302
VERSION     NZ_CP154302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41076128
            Assembly: GCF_039519935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Xu,C.
  TITLE     Antimicrobial Susceptibility, Virulence, and Genomic Features of a
            Carbapenem-resistant and Hypervirulent Serotype K25, ST11 Klebsiella
            pneumoniae in Ningxia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Xu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2024) School of Clinical Medicine or Laboratory
            Medicine Department, Ningxia Medical University, 692 Shengli Street,
            Xingqing District, Yin Chuan, Ningxia 750000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. v0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039519935.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 08:15:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,995
            CDSs (total)                      :: 5,873
            Genes (coding)                    :: 5,685
            CDSs (with protein)               :: 5,685
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 64 of 188
            Pseudo Genes (incomplete)         :: 133 of 188
            Pseudo Genes (internal stop)      :: 25 of 188
            Pseudo Genes (multiple problems)  :: 34 of 188
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP154302.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP156"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="pKP156-4"
                     /geo_loc_name="China: Ningxia"
                     /collection_date="2022-09-29"
     gene            complement(1259..1408)
                     /locus_tag="AAHG17_RS27405"
                     /old_locus_tag="AAHG17_27410"
     CDS             complement(1259..1408)
                     /locus_tag="AAHG17_RS27405"
                     /old_locus_tag="AAHG17_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1493..1750)
                     /locus_tag="AAHG17_RS27410"
                     /old_locus_tag="AAHG17_27415"
     CDS             complement(1493..1750)
                     /locus_tag="AAHG17_RS27410"
                     /old_locus_tag="AAHG17_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1760..3445)
                     /locus_tag="AAHG17_RS27415"
                     /old_locus_tag="AAHG17_27420"
     CDS             complement(1760..3445)
                     /locus_tag="AAHG17_RS27415"
                     /old_locus_tag="AAHG17_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(3550..5037)
                     /gene="ltrA"
                     /locus_tag="AAHG17_RS27420"
                     /old_locus_tag="AAHG17_27425"
     CDS             complement(3550..5037)
                     /gene="ltrA"
                     /locus_tag="AAHG17_RS27420"
                     /old_locus_tag="AAHG17_27425"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            5682..5927
                     /locus_tag="AAHG17_RS27425"
                     /old_locus_tag="AAHG17_27430"
     CDS             5682..5927
                     /locus_tag="AAHG17_RS27425"
                     /old_locus_tag="AAHG17_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6201..6572
                     /gene="tolA"
                     /locus_tag="AAHG17_RS27430"
                     /old_locus_tag="AAHG17_27435"
     CDS             6201..6572
                     /gene="tolA"
                     /locus_tag="AAHG17_RS27430"
                     /old_locus_tag="AAHG17_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6569..6934
                     /locus_tag="AAHG17_RS27435"
                     /old_locus_tag="AAHG17_27440"
     CDS             6569..6934
                     /locus_tag="AAHG17_RS27435"
                     /old_locus_tag="AAHG17_27440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7109..7387
                     /locus_tag="AAHG17_RS27440"
                     /old_locus_tag="AAHG17_27445"
     CDS             7109..7387
                     /locus_tag="AAHG17_RS27440"
                     /old_locus_tag="AAHG17_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7375..7689
                     /locus_tag="AAHG17_RS27445"
                     /old_locus_tag="AAHG17_27450"
     CDS             7375..7689
                     /locus_tag="AAHG17_RS27445"
                     /old_locus_tag="AAHG17_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7853..8281
                     /locus_tag="AAHG17_RS27450"
                     /old_locus_tag="AAHG17_27455"
     CDS             7853..8281
                     /locus_tag="AAHG17_RS27450"
                     /old_locus_tag="AAHG17_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8307..8486
                     /locus_tag="AAHG17_RS27455"
                     /old_locus_tag="AAHG17_27460"
     CDS             8307..8486
                     /locus_tag="AAHG17_RS27455"
                     /old_locus_tag="AAHG17_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8513..9043)
                     /locus_tag="AAHG17_RS27460"
                     /old_locus_tag="AAHG17_27465"
     CDS             complement(8513..9043)
                     /locus_tag="AAHG17_RS27460"
                     /old_locus_tag="AAHG17_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9050..9781)
                     /gene="mobC"
                     /locus_tag="AAHG17_RS27465"
                     /old_locus_tag="AAHG17_27470"
     CDS             complement(9050..9781)
                     /gene="mobC"
                     /locus_tag="AAHG17_RS27465"
                     /old_locus_tag="AAHG17_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(9781..11745)
                     /locus_tag="AAHG17_RS27470"
                     /old_locus_tag="AAHG17_27475"
     CDS             complement(9781..11745)
                     /locus_tag="AAHG17_RS27470"
                     /old_locus_tag="AAHG17_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8445
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          578..1156
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9054..9781)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tgcggtcttt tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac
       61 ccgcaaaatc aggagcagct ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg
      121 tgggtggtca ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca
      181 gccggacaga gccagaaaga ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa
      241 gcaaggtctt ttcccctgaa gtcagccacg ccccagcggg gatgacttgc atttctccgc
      301 aaggagaaaa aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg
      361 ttcacctatc ctgataattg atcgtcaggg aatagaaaga cttaatcagg ggacaatatc
      421 ccacatcagc gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc
      481 actcggtcgc tacgctccgg gcgtgagact gcggcgggcg ctgcggacac atacaaagtt
      541 acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacgg cagggccgcg
      601 ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa cagacgcttt
      661 ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc
      721 cgacaggact taaagatccc caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt
      781 tccgaccctg ccgtttaccg gatacctgtc cgccgttctc ccttgcggga agtgtggcgc
      841 tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg
      901 ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact
      961 tgagtccaac ccgggaaagc acgcaaaacg ccactggcag cagccattgg taactgggag
     1021 ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg
     1081 gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta ccacggttaa
     1141 gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt tttttcgttt
     1201 acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1261 actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt ccgccctgaa
     1321 catcccggat ataatttgcc tgacatgcgg ccagaagaaa gccagcaaca atcaatataa
     1381 aaagaaaaat agtctttacc tttttcatat actttccctg tatttaaaga ctccggcatt
     1441 catccctgaa caccggaagt cagtatttca aatcatcctt gataattttt tgttaccagt
     1501 taccgtcccg ataatcaaaa gctatataac aattatccac atcaggtttt atttgatttt
     1561 taaaaaatgg ctgtagtaaa ggaacccatt ctttcttcaa atcaaactca ccattattaa
     1621 tattatcctt taaaggcatt cccaagcttt caatgacaga accatcgtca ccaaagtctt
     1681 tagaatactc ttccccttta aactcttcag ttttcttatc aaaccatgtc aaacgtaatt
     1741 taagccccat atttacctct caaagatatt ttttaatgtt ccgttttgga tctggccctt
     1801 taacctgctt acccgttttg ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat
     1861 acccttcaag ctcaccgtgc tgggaatccc attcataaat tttacgtttt ttatcgccat
     1921 accatcgggc ccgcttacca ccaccacctt gtttaggtgt tttaggatca cctcgcttta
     1981 actctccaag tccctttatg tcttctgttt taggtactgg atgataatca tggccgtagt
     2041 ctttagtccc cttgcgaggc ttgttctttt cctcatttaa cttattttct gcatccttcg
     2101 cttttttctc cttttgtttc cggctctcca tcgccccggt aagtgcagcc ttatctctgg
     2161 ataaggattc attcattgcg tttaaagatg cctgagccgc ttttaatttc tcattctgat
     2221 tctgaatatc cttatcaaga tagcgaacct gatcctgcca cccggagcca tattcatgag
     2281 gcgggttata agtcacaaaa tcatctttat ttgcctctgc ctcttttacc tttttatcgg
     2341 cttcctgaac agcagccctt gcaccgggga gcgccttctc tgtcgatgca atgcggctgt
     2401 ttaaggtcgc aatattttta tcttcggcat ccagctcagc tttcgcttta tcatagtctc
     2461 ttttcagccc ctcttccgga tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct
     2521 cctgacgctg ttttacctgt gccggggtaa gaacatctgt caccgacaca taaaccggct
     2581 tctgtccggt ctctttcggg aaacgaataa cggcctcacg ggagttacca ccggctgtaa
     2641 aaccagccgg acgtgaatca cctttttcag caacaatgcc ttttggcggg gctttcgctg
     2701 tcggaacacc tttcggtacg ctcacctgca gagacgggag acccggaatc gacacactga
     2761 atacccccgg acgttttgtc ggtttcgcat ccaccacagg gacactcatt ggccgacctg
     2821 cgacaaccgc aatatgctgc cgctcatcct tcaccacatc cacaacccgt tgtctgacac
     2881 gaaccgtcgc ctggtccagt ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg
     2941 gtaatgacgt cataattttt gacatcattt tcggatcatc ttttgcaatc tcagaaggca
     3001 gcacaccgta gatcgcaatc ccccacagac caaacttaaa cggccccttc agggcagcca
     3061 gcacatcagc aacggcggcc gacaacgcat cgccggaaac tgataaagcc agtccttcag
     3121 cccccggagt agccagggca ggcagaccga aggccatcgc agtggccgaa gactgtccgc
     3181 caccagagct accgctatga ttgccgctgt taccaccatt accaccgggt ttactcccgc
     3241 cgttaccaaa ctcccccgga ttatagttat gaatatctcc attcggcgta tgcgtcgtac
     3301 cccagcccgg cccgctgttt ggatccgtac caccacctga tgaagggcca cctttaccag
     3361 acgtaccgtt cacattccca ctggcctgac caccattatg tcccagacct gaattacccg
     3421 gacctcgacc gtctccacca ctcataccgt tttcctcttt gacatttaaa acaataaatt
     3481 aaagccaaca tgtcgcggta gggatacccg ttaccggata ccccccgcac agatcccggc
     3541 gtgcgcgatt tacgcaccgg gctcctgcct cgggtgtctg gcggtgaacc gctccacagg
     3601 ccatggatga agaacccgaa cccttggtag ccatgcggct gccagtttgt ttgctttcgt
     3661 ccaggtcgta tcatccttct ggctcctgcg cctgagcgcc cggcgccaga ggtttgttac
     3721 gtgtgtcctg aacttctgca tggtggggaa gttgcccggt accgagtgat agttcaggta
     3781 tccctgaacc actctcctga gccattttcc ctgttcgggg attgagtaat gccagcgcct
     3841 tcgcagaccg tctttgatgg ctttcagagt tgccgtcatc cgatcccggc gggtctttcg
     3901 tatcagcatg aacctgccgt tgcgatcttt cccgctgatg tgcgtgaacc cgaggaagtt
     3961 gaacgtttct ggtttgcctt ttcccctgat ggcacggttt tcggcagcga agcggccgaa
     4021 ctccatcaga cgggttttct ccgggtgaac cgtgagtccg aactccctca gtctgcgctg
     4081 catggctata cggaagcgcc gggcatcgta tcgtttgtcg aacccgatga cgatgtcatc
     4141 ggcgtatctg accattacca cattgcctgt ggcatagcga cgtcgccact gatgcgccca
     4201 cagatcgaag acgtagtgga ggtatatgtt tgccagcagc ggtgagatga ccgcaccctg
     4261 tggggtgcct tcctccgttg ctcgccattg accctcctcc gacgtcccgg ctgtgagcca
     4321 cttacgtatg agcctgatta ccctccggtc gccgatccga tgctctgtga acctgatcag
     4381 ccattcgtgg ctcaccctgt cgaagaactg actgatgtcg gcatccagta cccagtttac
     4441 gttagtgcgt accagccctg tggccagtgc gtccagtgca tcgtgctggc ttcgcccggg
     4501 tctgaacccg tatgagaacc ccataaagtc gttttcatag actgcgttca ggattttcac
     4561 cagcgcatac tggacgatct tgtcctccag cgaggcgatg ccgagcgggc gttgttttcc
     4621 atccgctttt gggatgtagt gacgcctgcc gggctgcgcc ctgtagctgc cctgatgtag
     4681 cctccggtgc agatctgtta tgttgttctt catgtttccg gcgtagtcca tccacctgat
     4741 gccatccact ccggcggccg ctttcctgct cagggagagg aatgcggctt ccagtgcttc
     4801 gactgtcagc aggtggaaca atgctgtaaa ccgttctttc ttccgctgct tcgcagcttc
     4861 ccgcacgcgt gacagcctct gtgacatgct ttcccggctc tgtgtccggc gcatgtgtgg
     4921 ctgttccgcg ttccccttgg ccccgctcct tcgctccact gactccgctc ctttcgggtt
     4981 gttcgcctgc ttcgccgcta ctatgagcga gtccgacttc tcctctccgt acatcaccgg
     5041 ctatgactcc tcgtcttccc ggtgcgggcc atctccgaca ctggcagatg gtcagagggg
     5101 agatctcccg gttcccgcgt agagatcgta ttgacatgcc agggtctcag accccgccgg
     5161 gtccatgtgg cactcgcagt gtcgcaccct atgatgttgc cttccgttaa cagtacaacg
     5221 tcggcacccg gtaatttaat atacatttcg tggctcaatg gctggcctgt caacacccct
     5281 gtcaacgctt cgccccatac ctcgcggtat gcaacgcatg actcggggac cttgtggatt
     5341 gctggtcctt caatggtcgg ggactttcac cccttgatct ctaaccggtc tcccggcaca
     5401 cactgtacat atacacagta taattattac cttactcctc ccgttttcag gtgtcaatat
     5461 atgagattag ttctcatctt caggcacaaa aaagcgcccg gagccacctg taagcagatc
     5521 gcgtcttttc ccgaaaattt ctcatggcaa caaattttat tatttaagac attcatcaca
     5581 cttacatttc agatggtata gacacatctg aaagctaact ggcatgagga gaggacctgc
     5641 ggttaaactg ttcgggaaac ctgagacgcc cggaggccac tatgacgcac ctgaaactga
     5701 ctcccgctga agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg gccatgggcc
     5761 tgaaagggaa caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc
     5821 gtgccattca ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg
     5881 aggctacgga aaccaccttc acctggcagc cacagcgccg ccggtaaggg gtatttacca
     5941 taacgtcgca ttgcccgcac cgctcagacg gtctgagggc gtttccggcg gctacgggat
     6001 aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa ttgtaacccg
     6061 ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg atacgataag
     6121 ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa atatacatgt aacatttttt
     6181 actgacaggt catcaacccg atgcgtttaa taccctttat cctgctggca ttgtgcagcc
     6241 ccttactcct gacggcctgt aagcattcca ccacccagcc gccgaaaatt tatactctgg
     6301 ataagtcatc taaaatcagt agctacgctg cggccatcaa gaccgctgtt gaaatgcaga
     6361 tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg
     6421 atggcactct gaacagcgcc attgctgaag gaggagatcc gaaactctgt aaagcagcaa
     6481 tatcagccgt tacccacgcc aaaattccgc cggcaccgga tgaaaatacc tggcaaatat
     6541 tcaaaaacgc ccctctggat ttcagaccat gaaagctttt actctgacag tgctggcggc
     6601 catgttcctc actccggtac atgcgatacc actaaaaccc gtaaccacac tgcagccaac
     6661 ctacccggta caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat
     6721 tctggacgat ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga
     6781 aacgctgacg gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt
     6841 tataaatttt gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac
     6901 ccaaaagctc aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa
     6961 tagtcctgga tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa
     7021 tgggcagcaa aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg
     7081 taatacatat gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga
     7141 agaacttaag gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct
     7201 aatgctggag gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg
     7261 ggacggacag gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga
     7321 agcggaagcc tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca
     7381 caggtaacga tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa
     7441 accaaaaatc ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa
     7501 ctgaaaacat tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa
     7561 ctggtgatcg ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg
     7621 gaccagattg tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac
     7681 agcctctaat aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa
     7741 gcgcagcgcg tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat
     7801 ttaccgtgag aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa
     7861 aacattgctc aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata
     7921 caatcatgta aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt
     7981 ggatgtgtca gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc
     8041 cggcatttca caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat
     8101 gcaaaaggaa ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc
     8161 ccgggaagaa gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga
     8221 cagattgaca acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg
     8281 agaacttacc gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg
     8341 ctaaattcat taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg
     8401 ataccccggc cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga
     8461 tgcgggataa gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt
     8521 tgccgaacgg ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt
     8581 taaaatagcg gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc
     8641 tttccccgtg ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact
     8701 tcccggcatc aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga
     8761 ggtaatcaca cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg
     8821 tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt
     8881 tcatcatcgc cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc
     8941 aggcgagcac caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt
     9001 ctttgtcttc cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg
     9061 tcataaattt aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct
     9121 cgccggtctg tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa
     9181 gcgtcggccg gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga
     9241 acagataccc ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct
     9301 caaatgccac cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca
     9361 gggccatacg gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca
     9421 gggactgcac ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg
     9481 gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca
     9541 gcccccacac gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt
     9601 tcgccaccag actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc
     9661 gcgcaaccag ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg
     9721 ttttctccgc gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc tccagtgtca
     9781 tcagtaaagc cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg ccggaggcag
     9841 gtactccgcc agcgggtccg ttttcgccag caccgggaag gttttcagcg aaatttcccg
     9901 gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac
     9961 cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt
    10021 ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc tggcccggcg
    10081 gcgctccacg tccacccgga tctccccggt ctggcccgcc gcccactgcg cggactcctg
    10141 gctggccgcg cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa
    10201 atcagcgggc agatcctgcc cgcactgacc gaagtcgccc agcgactggt gcgcgagcag
    10261 caggttgcag tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag
    10321 atatttaatc tcatcgagca tgatgctcgc gtgcggccac ctccggaact catcccgcgc
    10381 aatgatgatt tgcgcgcagc gggcaaacag catcttctgc acccggatca ccgcctcatc
    10441 gtccatcgag ccgatgacgt acaggcagcc gccgccgttg attatccccg ccacatcgat
    10501 accgctgtcc gtctgcagga cgctcaggtc ggccacgttc tccagcttgg tgatcagccc
    10561 cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc gccgccagca cctgctgaat
    10621 gtttgccccc tgcgggaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg
    10681 gtagtggtcc gccacgtcgc cggtctcact caggttaaac ccggcaacca gcagattctt
    10741 cagggcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag
    10801 atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc
    10861 tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt
    10921 ggtggccatc gcgctcttgc cgctcccggg caggccgagg atctgaatat tggtcttgcg
    10981 ccaggtatca cgagggacca gcaccggctg gccctgctca tccttaccca gaaacaccgc
    11041 ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg gtaccggctc
    11101 cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc cgctggctgc tgcgccggct
    11161 ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac catcaagcag
    11221 caggaacgcc agcagccgcg ccagaaggac gccggcgaca agccctccgg cacaccaggc
    11281 tatcccgaac aggttagccg gctgcagcag gtaataatct gcgttcgcat agaggtccgg
    11341 gcgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc cccccagaag
    11401 ggccagaccg gcagcgccgg tcagccagta cgcgtagttc cagacatgtc gccgtgccct
    11461 gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag tccggttatt
    11521 atcgcgctta tagcgatcgg catactcccg ccgcgcctta tggctcagcc ccacaaaacg
    11581 cattcggccg gcccagagtt cagccagacc gaaaaacagg ccgataacca gaaaaaaacc
    11641 ggccagactg taatactccc ggtacagcca gctgacccac gggtaaatct gccgggccag
    11701 ctccgggata tgccactgcc aggcaagcga atccgtatta aacatgcaat ctctctcctt
    11761 cagacgacgg gtttaccggc gtgatgcgca gcccccgctg cacaagccgc ggacatgccc
    11821 caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca ccgcggaggc
    11881 cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg agcacgcccc
    11941 agcgtgtgag aggaaaaggc ggcaccccag
//
