LOCUS       NZ_CP152452             5596 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Klebsiella pneumoniae strain JXLV-KP19 plasmid p19-5, complete
            sequence.
ACCESSION   NZ_CP152452
VERSION     NZ_CP152452.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41075591
            Assembly: GCF_038979805.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qiu,J. and Liu,Y.
  TITLE     The UvrD helicase regulated the copy number of the virulence plasmid
            and contributed to the heterogeneity of ST11-CR-HvKP virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Qiu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2024) Department of Clinica Laboratory,Medical
            Center of Burn Plastic and Wound Repair, The First Affiliated
            Hospital of Nanchang University,Nanchang University, Yong wai zheng
            jie No.17, Nanchang 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio Sequel2 hifi
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038979805.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 04:32:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,945
            CDSs (total)                      :: 5,823
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 64 of 167
            Pseudo Genes (incomplete)         :: 112 of 167
            Pseudo Genes (internal stop)      :: 22 of 167
            Pseudo Genes (multiple problems)  :: 31 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152452.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JXLV-KP19"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p19-5"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2020"
     gene            complement(join(5504..5596,1..258))
                     /locus_tag="AAHF15_RS29675"
                     /old_locus_tag="AAHF15_29675"
     CDS             complement(join(5504..5596,1..258))
                     /locus_tag="AAHF15_RS29675"
                     /old_locus_tag="AAHF15_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(324..869)
                     /locus_tag="AAHF15_RS29680"
                     /old_locus_tag="AAHF15_29680"
     CDS             complement(324..869)
                     /locus_tag="AAHF15_RS29680"
                     /old_locus_tag="AAHF15_29680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(895..1221)
                     /locus_tag="AAHF15_RS29685"
                     /old_locus_tag="AAHF15_29685"
     CDS             complement(895..1221)
                     /locus_tag="AAHF15_RS29685"
                     /old_locus_tag="AAHF15_29685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1579..1737)
                     /locus_tag="AAHF15_RS29690"
                     /old_locus_tag="AAHF15_29690"
     CDS             complement(1579..1737)
                     /locus_tag="AAHF15_RS29690"
                     /old_locus_tag="AAHF15_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2377..2553
                     /locus_tag="AAHF15_RS29695"
                     /old_locus_tag="AAHF15_29695"
     CDS             2377..2553
                     /locus_tag="AAHF15_RS29695"
                     /old_locus_tag="AAHF15_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2543..2887
                     /locus_tag="AAHF15_RS29700"
                     /old_locus_tag="AAHF15_29700"
     CDS             2543..2887
                     /locus_tag="AAHF15_RS29700"
                     /old_locus_tag="AAHF15_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3096..>3530
                     /locus_tag="AAHF15_RS29705"
                     /old_locus_tag="AAHF15_29705"
                     /pseudo=""
     CDS             3096..>3530
                     /locus_tag="AAHF15_RS29705"
                     /old_locus_tag="AAHF15_29705"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3565..4074
                     /locus_tag="AAHF15_RS29710"
                     /old_locus_tag="AAHF15_29710"
     CDS             3565..4074
                     /locus_tag="AAHF15_RS29710"
                     /old_locus_tag="AAHF15_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4081..4293
                     /locus_tag="AAHF15_RS29715"
                     /old_locus_tag="AAHF15_29715"
     CDS             4081..4293
                     /locus_tag="AAHF15_RS29715"
                     /old_locus_tag="AAHF15_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4960..5184
                     /locus_tag="AAHF15_RS29720"
                     /old_locus_tag="AAHF15_29720"
     CDS             4960..5184
                     /locus_tag="AAHF15_RS29720"
                     /old_locus_tag="AAHF15_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            5168..5434
                     /locus_tag="AAHF15_RS29725"
                     /old_locus_tag="AAHF15_29725"
     CDS             5168..5434
                     /locus_tag="AAHF15_RS29725"
                     /old_locus_tag="AAHF15_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          2212..2291
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1629..2026)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2878..4409
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cataataaaa gacattatgg cctcagagtt atcatcagaa gcagctctct gcattactac
       61 ttcgattccc ttttctttga atctataagt atccagttgc ctattaaact tagaaagacc
      121 tacaaccata cttagtttgt tatctttaac agtaagagat gcttcctgag taccattaca
      181 tcggtagtaa gcggtgtaac cgtcagcaaa agatcgaccg gggaatataa atgctaggaa
      241 cgcaattaac caatacatag ttgagaatct tggtattaca catttcacta tcctctcttt
      301 catttttcgt tcaccatact ttgttatctg atttttaatt tttctatttt ataaaccaag
      361 tctctaccaa aaaggcgaaa attatcctca aataataata attgtttaag aacatccatg
      421 aaagcagctg cttcaccctc agttatggga gctactctat ataacatata acctgagtta
      481 agggtttctt ttatactttc ttcaatgctc attataaata ttagtggatt atcatttttc
      541 ttcccccatg ggtttctaat taatccttcg atgtactcag gtcctttgcg tcctattgcc
      601 tgcgcaatat tttttatttc atctgcgtga gggctttgtt gtacagcttt gtttatgcct
      661 gcttgagtga ttttcagaga ctctacaccg tgtatgttgt atgagataaa atgtaataaa
      721 ttaagatgca atgcaattaa tccatttttg tttacatctg agtatttgga taaaaaacgt
      781 aatgaagtgt taactctccc ttgaagccta acaattggga gtgctgcctt gtatatttcc
      841 ttctccgtta aataccgttt aaaaaacata attctccgcc tccgtatagc gtgcctattt
      901 tatttcgtcg taaaacttag ctctgaaata ttcctttaga accatcatgt cattaataat
      961 tgaatctatc tcatccattt tttcttggta ttcttttaat ggtgaaggga gcggtaactc
     1021 aatatttgaa aagtgattgg cgagataggg atcaaggttt ctcatccatt caggcttttc
     1081 gccaatgcgt tcttcaactg aaagtgaacc tgataatctc tggttaataa gatcacttag
     1141 actcattttg gttttattcg aaagccgttg taaggattgc tttaatccta tatctagcat
     1201 gatatttgtt ctttctctca ttaccccccc ttgctattgc gttttttgtt gcgcacaaag
     1261 ctaccattgc gcattaagaa acgcaataaa aaaggaggga gggagtggtt tgtacatgat
     1321 gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg tgggatggat ggagctccgg
     1381 ggataacaga actgacgatc atttaaagga tcttcttgag atcttttttt tctgcgcgta
     1441 atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc
     1501 agttggggaa ctgcttaacc gggtaactgg gtatacgaga ccgcagatac caaaactgtt
     1561 ctttcagtgt agccgcagtt aggcacccac ttcaagacct ctcaatatct ctctcgtata
     1621 tatccagtta ccaatggctg ctgccagtgg cgtttttgcg tgtccgtccg ggttggactc
     1681 aagatgatag ttaccggaaa gggcgcagca gtcgggctga acggggggtt cgtgcataca
     1741 gcccagcttg gagcgaacga cctaaaccga gccgagatac cagcagcgtg agctatgaga
     1801 aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt atccggtaaa cggcagggtc
     1861 ggaacaggag agcgcacgag ggagccgccg gagagaaatc accggcatct ttaagtcctg
     1921 tcgggtttcg tcctcctctg acttgagcgt ccatttctgt gatgctcgtc aggggaggcg
     1981 gagcctatgg aaaaacggca acgccgcatc cttttctccg gccctttctg atttgagcgc
     2041 ctttgcttta tcccgttatc cgtgagtcgg tcacgcccgc cgcagccgaa cggcaggagc
     2101 gcagctaccg agtgagcgag gaggcgtcat ttcatctgtc cgtgcatggt attgcggacg
     2161 ctcctgagcc acatatcaca cctgccacat gaggcacttt catgaatcgt gctctcatgc
     2221 caacatagta agccagtata cacagcgtaa acgctgtgac tggttcaggg ctgcgccccg
     2281 aaacccgcta acaccactga cgcgctggcg cttgtccaac gccggagcga cccggaaaac
     2341 ttttccgtgc cgtccggcgt ttgcgaagga gggataatgc agaagccgaa gaagaagccg
     2401 ctcgtcgtaa aaaaaagatc ctggcatccg gcagtgatgg caaatctgat gcgtcaggca
     2461 caggaggaag attaccgccg ggagcagatg gcagagcttg agcgttcagc ccggtcacat
     2521 tttgcggata tcggaggaga ggatggcggt taagcgtacc cgttttttag gtatccgggt
     2581 gaccgatggg gagtaccagc agctgctgga gcgctgcaat ggaaggcaac tggcggtgtg
     2641 gatgcgggaa acctgtctcg atacgcgccc ggcgcggtcg ttgcggctgc cgtccatcga
     2701 tccggttttg ctgcgccagc ttgccgggat ggggaacaac ctcaaccaga tcgcccggaa
     2761 aattaacggc ggccagtggt caggcgctga ccgggttcag gtggtggccg cgctgatggc
     2821 cattgatgcc gaactcgagc ggctgcggca tgccgtgctg gaaaaggggg cagacgatga
     2881 tcgttaagtt tcatccgcga gggcgaggcg gcggtgccgg gccggtggat tatctgctgg
     2941 ggaaagaccg ccagcgcgac ggggccatcg tcctgcaggg aaagccggag gaggtccggg
     3001 agcttatcga cgcctcgccc tacgccaaaa agtacacctc cggcgtcctg tcttttgctg
     3061 aacaggattt accgcccggc cagcgtgaaa agctgatggc gagcttcgag cgggttctga
     3121 tgcccggact cgataaagac cagtacagcg tgctgtgggt tgaacaccgg gacaaggggc
     3181 ggctggaact gaattttctg gtaccgaaca cggaactgct gaccggcagg cgtctccagc
     3241 cgtactacga ccgggcagac cgtccgcgca tcgatgcgtg gcagaccgtg gtgaacgggc
     3301 gactggggct gcacgacccg aacgcaccgg agaaccggcg cgcgctggtg acgccatctt
     3361 cgttacccaa aacgaagcag gaagccgcag aggctattac gcggggctta ctggccctcg
     3421 cctcgtcagg ggagcttaaa acgcgtcagg acgtcactga ggcgctggaa agcgcaggtt
     3481 ttgaggtcgt gcgcaccaca aagagcagca tcagcattgc cgacccggac ggagggcgaa
     3541 acatccgact taagggagcc atctatgaac agtcttttaa cgctggcgaa ggacttagag
     3601 cagaaatcga aagcgcagca gcagagtacc ggcgaaatgc tgaaagccgc attcagcgag
     3661 cacgagaagt ctgtcagagc ggaactgagc gaaagcggga agagaatcag cgccgccatc
     3721 cgcgcccacg agcagggtat gagcgcggcc atgcagtcga accgcctgag cgtgatgcgt
     3781 atggtcgggc ggacatggct gaccatcacg atggtgtcag gactgctgtt cgccagcctg
     3841 agcggggtgt tgtggtatca ggggagcttg atagcgtcaa atctggccga aatcgaccgg
     3901 cagaacgcag cgctgtcgaa gctgaacgcg aagacctggg gcgtgacgta cctggaagac
     3961 agcaacggac gttttctggt gctgccgaag gggacggccg tagacaggac gcagagctgg
     4021 acggtcggga acgggcggtc gaagcagaac gcgctgagac tggtgaagga gtaagtgccg
     4081 atgaccgagc tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg
     4141 aaaaggctgg acgagtggga gagcgccttc ggggaatggc ggaaaatgtc agggcttatg
     4201 caacgggaga acgcggcgct gagcgagcgc gtgacgcgct tgagtcagca ggtggaacgc
     4261 ttgagcgggc aactgcagcg tttgagccgg tagtgcagcg tcatgagatg gccgtggccg
     4321 cagaacgggc gcacgagcaa cggcagcacg aaaaggaact gacggaagcg cgttcccggc
     4381 agcgaagcta tgacggtccg tcgctgggct gaaatgcagg aaaacagggt tctcatcggg
     4441 catttacgct gaaaccggat gtcattcacc tggctgcatg gctatgcagc caggtaaaat
     4501 ttatctgtcg cgaacggggc ggtttatcgg gtggtttgtt gccggtttta cctgactgcc
     4561 accccgttcg cggcgaaccc gtccggggcg gtgcgggcaa cgggtgttat gtttaatcag
     4621 cgtgaacggc agatacaaaa aagccccgga gggcttttaa ctactgactt atttttttga
     4681 agatttgctg ggtttcaatt tgtcagtatt tcccggcgtt tttttattgt cacgctggcg
     4741 tttatcaggt tcccctgttg aatcggcaat tcgtttcgga cccggcttaa gacccataat
     4801 cgttatcctt tcaatagtta agaggaaagc cctcaagcta gatatattga ccgcgttacg
     4861 gattatcaag aggggctaac aagattattt tttgttcttt taggttgatt gtggtactgt
     4921 tgatatacat gtatagcaaa tggtgttcgg gaggtttata tgcttgctat ccggttatct
     4981 gatgagattg agtcccgtct ggactcgctg gcgaagcaaa ccggcagaac aaagacgttt
     5041 tatgcgcggg aagcaatact ggcgcatctg gaagacctgg aggattatta tctttcagca
     5101 gaaactgctg cacgcgttcg ccgtggtgat gaagcagtgc attcgtctga agacgtgagg
     5161 aagtcacttg gtctggacga ttaactattc cgatcgggcg ctcaaatcgt tacgcaagat
     5221 ggacaaacag aacgcacgac ggattgtgga ttttatgagt ttacgcattg cagttgctgc
     5281 cgatcctcgc cagtcaggga agccgctcaa aggtgagctg ggcgagttct ggcgctatcg
     5341 ggtgggagat tatcgcgttc tgtgtgagat ccgagatgac gagcttgtta tccttgccgc
     5401 cacgattgga catcgccgcg aagtttacga ctgaagcccg ctatgcgggc ttttttgatg
     5461 ccgacaacag gtattatggt aaatactcga ctaactacgt gttctactgt ttgtcggttt
     5521 tagttaacca gcaggttgtc ttaacgataa tgtagccgtt atttttaact aataaggtca
     5581 tattgttaac gtcagg
//
