LOCUS       NZ_CP152448            11970 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Klebsiella pneumoniae strain JXLV-KP19 plasmid p19-1, complete
            sequence.
ACCESSION   NZ_CP152448
VERSION     NZ_CP152448.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41075591
            Assembly: GCF_038979805.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Qiu,J. and Liu,Y.
  TITLE     The UvrD helicase regulated the copy number of the virulence plasmid
            and contributed to the heterogeneity of ST11-CR-HvKP virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Qiu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2024) Department of Clinica Laboratory,Medical
            Center of Burn Plastic and Wound Repair, The First Affiliated
            Hospital of Nanchang University,Nanchang University, Yong wai zheng
            jie No.17, Nanchang 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: PacBio Sequel2 hifi
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038979805.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 04:32:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,945
            CDSs (total)                      :: 5,823
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 64 of 167
            Pseudo Genes (incomplete)         :: 112 of 167
            Pseudo Genes (internal stop)      :: 22 of 167
            Pseudo Genes (multiple problems)  :: 31 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152448.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JXLV-KP19"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p19-1"
                     /geo_loc_name="China:Nanchang"
                     /collection_date="2020"
     gene            1476..3440
                     /locus_tag="AAHF15_RS27115"
                     /old_locus_tag="AAHF15_27115"
     CDS             1476..3440
                     /locus_tag="AAHF15_RS27115"
                     /old_locus_tag="AAHF15_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3440..4171
                     /gene="mobC"
                     /locus_tag="AAHF15_RS27120"
                     /old_locus_tag="AAHF15_27120"
     CDS             3440..4171
                     /gene="mobC"
                     /locus_tag="AAHF15_RS27120"
                     /old_locus_tag="AAHF15_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4178..4708
                     /locus_tag="AAHF15_RS27125"
                     /old_locus_tag="AAHF15_27125"
     CDS             4178..4708
                     /locus_tag="AAHF15_RS27125"
                     /old_locus_tag="AAHF15_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4735..4914)
                     /locus_tag="AAHF15_RS27130"
                     /old_locus_tag="AAHF15_27130"
     CDS             complement(4735..4914)
                     /locus_tag="AAHF15_RS27130"
                     /old_locus_tag="AAHF15_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4940..5368)
                     /locus_tag="AAHF15_RS27135"
                     /old_locus_tag="AAHF15_27135"
     CDS             complement(4940..5368)
                     /locus_tag="AAHF15_RS27135"
                     /old_locus_tag="AAHF15_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5532..5846)
                     /locus_tag="AAHF15_RS27140"
                     /old_locus_tag="AAHF15_27140"
     CDS             complement(5532..5846)
                     /locus_tag="AAHF15_RS27140"
                     /old_locus_tag="AAHF15_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5834..6112)
                     /locus_tag="AAHF15_RS27145"
                     /old_locus_tag="AAHF15_27145"
     CDS             complement(5834..6112)
                     /locus_tag="AAHF15_RS27145"
                     /old_locus_tag="AAHF15_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6287..6652)
                     /locus_tag="AAHF15_RS27150"
                     /old_locus_tag="AAHF15_27150"
     CDS             complement(6287..6652)
                     /locus_tag="AAHF15_RS27150"
                     /old_locus_tag="AAHF15_27150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6649..7020)
                     /gene="tolA"
                     /locus_tag="AAHF15_RS27155"
                     /old_locus_tag="AAHF15_27155"
     CDS             complement(6649..7020)
                     /gene="tolA"
                     /locus_tag="AAHF15_RS27155"
                     /old_locus_tag="AAHF15_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7294..7539)
                     /locus_tag="AAHF15_RS27160"
                     /old_locus_tag="AAHF15_27160"
     CDS             complement(7294..7539)
                     /locus_tag="AAHF15_RS27160"
                     /old_locus_tag="AAHF15_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8184..9671
                     /gene="ltrA"
                     /locus_tag="AAHF15_RS27165"
                     /old_locus_tag="AAHF15_27165"
     CDS             8184..9671
                     /gene="ltrA"
                     /locus_tag="AAHF15_RS27165"
                     /old_locus_tag="AAHF15_27165"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9776..11461
                     /locus_tag="AAHF15_RS27170"
                     /old_locus_tag="AAHF15_27170"
     CDS             9776..11461
                     /locus_tag="AAHF15_RS27170"
                     /old_locus_tag="AAHF15_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            11471..11728
                     /locus_tag="AAHF15_RS27175"
                     /old_locus_tag="AAHF15_27175"
     CDS             11471..11728
                     /locus_tag="AAHF15_RS27175"
                     /old_locus_tag="AAHF15_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11813..11962
                     /locus_tag="AAHF15_RS27180"
                     /old_locus_tag="AAHF15_27180"
     CDS             11813..11962
                     /locus_tag="AAHF15_RS27180"
                     /old_locus_tag="AAHF15_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4776..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(96..674)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3441..4168
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct tttgccctgt aaacgaaaaa
       61 accacctggg gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgtgg
      121 taactggctt tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg
      181 cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta
      241 ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg gttggactca agtgaacagt
      301 taccggataa ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg
      361 agcgaacgac ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact
      421 tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc
      481 gcaagaggga gcgacctacc ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc
      541 gtactgtcag attcatggtt gagcctcacg gctcccacag atgcaccgaa aaagcgtctg
      601 tttatgtgaa ctctggcagg agggcggagc ctatggaaaa acgccaccgg cgcggccctg
      661 ccgttttgcc tcacatgtta gtcccctgct tatccacgga atctgtgggt aactttgtat
      721 gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt gagcgaggag
      781 gcgcaatttc cccggcacct gcaagacggt caccggacgc gctgatgtgg gatattgtcc
      841 cctgattaag tctttctatt ccctgacgat caattatcag gataggtgaa ccatgtcggt
      901 ttttcgctga acattttacg ttaacctatc tctgttgctt ttttctcctt gcggagaaat
      961 gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa aagaccttgc ttacgctggc
     1021 tggacgccac cgacgcacgg tcttttcccc tctttctggc tctgtccggc tggacgccgg
     1081 ccacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca
     1141 cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc
     1201 gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc
     1261 gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg
     1321 catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg
     1381 accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac
     1441 gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct
     1501 ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc
     1561 gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggctg
     1621 aactctgggc cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg
     1681 ccgatcgcta taagcgcgat aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg
     1741 attcacccgg gcggatggtc agggcacggc gacatgtctg gaactacgcg tactggctga
     1801 ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct
     1861 ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc agattattac ctgctgcagc
     1921 cggctaacct gttcgggata gcctggtgtg ccggagggct tgtcgccggc gtccttctgg
     1981 cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac gaggcggtga
     2041 acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa atgacagacg
     2101 tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg
     2161 agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc
     2221 tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg
     2281 gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg
     2341 tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc
     2401 cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg
     2461 atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg
     2521 gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc
     2581 agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg
     2641 ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg
     2701 tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt
     2761 acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc
     2821 gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca
     2881 tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc
     2941 gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg
     3001 ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct
     3061 ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga
     3121 tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca
     3181 gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac
     3241 acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca
     3301 gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa
     3361 cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg
     3421 attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca
     3481 ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc
     3541 cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt
     3601 cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg
     3661 tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga
     3721 gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca
     3781 ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg
     3841 gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga
     3901 cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga
     3961 agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat
     4021 ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat
     4081 cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt
     4141 cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat
     4201 tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga
     4261 agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc
     4321 ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga
     4381 aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg
     4441 aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt
     4501 gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt
     4561 cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga
     4621 atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg
     4681 ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc
     4741 ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc
     4801 gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga
     4861 ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc
     4921 cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg
     4981 tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct
     5041 ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga
     5101 gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat
     5161 tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac
     5221 ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct
     5281 ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct
     5341 gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg
     5401 tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt
     5461 ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc
     5521 gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg
     5581 gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac atagccactg
     5641 tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac cgggcggcca
     5701 atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc tccttttctg
     5761 gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc
     5821 agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca gttccccagg
     5881 tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc tgatagtgct
     5941 gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt
     6001 cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt
     6061 tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac ataaaagccc
     6121 ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct atttgccaga
     6181 aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc tgctacaggc
     6241 tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac tggcggcaac
     6301 catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg tcatttttca
     6361 gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga tatagccagt
     6421 gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt
     6481 ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg atgcgttttg
     6541 ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt ggtatcgcat
     6601 gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc atggtctgaa
     6661 atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg gcggaatttt
     6721 ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc cttcagcaat
     6781 ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca tttttcccgc
     6841 ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg cagcgtagct
     6901 actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg tggaatgctt
     6961 acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta ttaaacgcat
     7021 cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct ttacggccac
     7081 gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc cgccatgcag
     7141 ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg cggttttccg
     7201 ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac cgtctgagcg
     7261 gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg gctgccaggt
     7321 gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc
     7381 acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg
     7441 cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg cgtccgccag
     7501 gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag
     7561 gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc
     7621 tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg ttgccatgag
     7681 aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt tttgtgcctg
     7741 aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag gtaataatta
     7801 tactgtgtat atgtacagtg tgcgccggga gaccggttag agatcaaggg gtgaaagtcc
     7861 ccgaccattg aaggaccagc aatccacaag gtccccgagt catgcgttgc ataccgcgag
     7921 gtatggggcg aagcgttgac aggggtgttg acaggccagc cattgagcca cgaaatgtat
     7981 attaaattac cgggtgccga cgttgtactg ttaacggaag gcaacatcat agggtgcgac
     8041 actgcgagtg ccacatggac ccggcggggt ctgagaccct ggcatgtcaa tacgatctct
     8101 acgcgggaac cgggagatct cccctctgac catctgccag tgtcggagat ggcccgcacc
     8161 gggaagacga ggagtcatag ccggtgatgt acggagagga gaagtcggac tcgctcatag
     8221 tagcggcgaa gcaggcgaac aacccgaaag gagcggagtc agtggagcga aggagcgggg
     8281 ccaaggggaa cgcggaacag ccacacatgc gccggacaca gagccgggaa agcatgtcac
     8341 agaggctgtc acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg tttacagcat
     8401 tgttccacct gctgacagtc gaagcactgg aagccgcatt cctctccctg agcaggaaag
     8461 cggccgccgg agtggatggc atcaggtgga tggactacgc cggaaacatg aagaacaaca
     8521 taacagatct gcaccggagg ctacatcagg gcagctacag ggcgcagccc ggcaggcgtc
     8581 actacatccc aaaagcggat ggaaaacaac gcccgctcgg catcgcctcg ctggaggaca
     8641 agatcgtcca gtatgcgctg gtgaaaatcc tgaacgcagt ctatgaaaac gactttatgg
     8701 ggttctcata cgggttcaga cccgggcgaa gccagcacga tgcactggac gcactggcca
     8761 cagggctggt acgcactaac gtaaactggg tactggatgc cgacatcagt cagttcttcg
     8821 acagggtgag ccacgaatgg ctgatcaggt tcacagagca tcggatcggc gaccggaggg
     8881 taatcaggct catacgtaag tggctcacag ccgggacgtc ggaggagggt caatggcgag
     8941 caacggagga aggcacccca cagggtgcgg tcatctcacc gctgctggca aacatatacc
     9001 tccactacgt cttcgatctg tgggcgcatc agtggcgacg tcgctatgcc acaggcaatg
     9061 tggtaatggt cagatacgcc gatgacatcg tcatcgggtt cgacaaacga tacgatgccc
     9121 ggcgcttccg tatagccatg cagcgcagac tgagggagtt cggactcacg gttcacccgg
     9181 agaaaacccg tctgatggag ttcggccgct tcgctgccga aaaccgtgcc atcaggggaa
     9241 aaggcaaacc agaaacgttc aacttcctcg ggttcacgca catcagcggg aaagatcgca
     9301 acggcaggtt catgctgata cgaaagaccc gccgggatcg gatgacggca actctgaaag
     9361 ccatcaaaga cggtctgcga aggcgctggc attactcaat ccccgaacag ggaaaatggc
     9421 tcaggagagt ggttcaggga tacctgaact atcactcggt accgggcaac ttccccacca
     9481 tgcagaagtt caggacacac gtaacaaacc tctggcgccg ggcgctcagg cgcaggagcc
     9541 agaaggatga tacgacctgg acgaaagcaa acaaactggc agccgcatgg ctaccaaggg
     9601 ttcgggttct tcatccatgg cctgtggagc ggttcaccgc cagacacccg aggcaggagc
     9661 ccggtgcgta aatcgcgcac gccgggatct gtgcgggggg tatccggtaa cgggtatccc
     9721 taccgcgaca tgttggcttt aatttattgt tttaaatgtc aaagaggaaa acggtatgag
     9781 tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag
     9841 tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc
     9901 aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa
     9961 tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa
    10021 tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc
    10081 tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg atgcgttgtc
    10141 ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg gtctgtgggg
    10201 gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga aaatgatgtc
    10261 aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa gtactttacc
    10321 actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga aggatgagcg
    10381 gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg atgcgaaacc
    10441 gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag
    10501 cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg
    10561 tgattcacgt ccggctggtt ttacagccgg tggtaactcc cgtgaggccg ttattcgttt
    10621 cccgaaagag accggacaga agccggttta tgtgtcggtg acagatgttc ttaccccggc
    10681 acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg acgccgctca
    10741 tccggaagag gggctgaaaa gagactatga taaagcgaaa gctgagctgg atgccgaaga
    10801 taaaaatatt gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc tccccggtgc
    10861 aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga
    10921 ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta
    10981 tcttgataag gatattcaga atcagaatga gaaattaaaa gcggctcagg catctttaaa
    11041 cgcaatgaat gaatccttat ccagagataa ggctgcactt accggggcga tggagagccg
    11101 gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg aaaagaacaa
    11161 gcctcgcaag gggactaaag actacggcca tgattatcat ccagtaccta aaacagaaga
    11221 cataaaggga cttggagagt taaagcgagg tgatcctaaa acacctaaac aaggtggtgg
    11281 tggtaagcgg gcccgatggt atggcgataa aaaacgtaaa atttatgaat gggattccca
    11341 gcacggtgag cttgaagggt atcgtgccag tgatggcgaa cacctaggcg catttgatcc
    11401 caaaacgggt aagcaggtta aagggccaga tccaaaacgg aacattaaaa aatatctttg
    11461 agaggtaaat atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt
    11521 taaaggggaa gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg
    11581 aatgccttta aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc
    11641 tttactacag ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc
    11701 ttttgattat cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg
    11761 acttccggtg ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa
    11821 ggtaaagact atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca
    11881 ggcaaattat atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact
    11941 gacggggatc gcggttcagt agaaaagatc
//
