LOCUS       NZ_CP152302             5114 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Shigella sonnei strain R16.0823 plasmid pR16.0823_5.1k, complete
            sequence.
ACCESSION   NZ_CP152302
VERSION     NZ_CP152302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123227
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: flye v. v2.9.2
            Genome Representation        :: Full
            Expected Final Version       :: No
            Genome Coverage              :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_039684345.1-RS_2024_05_20
            Annotation Date              :: 05/20/2024 11:58:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152302.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R16.0823"
                     /db_xref="taxon:624"
                     /plasmid="pR16.0823_5.1k"
                     /collected_by="Taiwan CDC"
     gene            1..936
                     /locus_tag="V8Z64_RS00005"
                     /old_locus_tag="V8Z64_25185"
     CDS             1..936
                     /locus_tag="V8Z64_RS00005"
                     /old_locus_tag="V8Z64_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024235833.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASVKA
                     LKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLRASE
                     RRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     gene            complement(1130..1576)
                     /locus_tag="V8Z64_RS00010"
                     /old_locus_tag="V8Z64_25190"
     CDS             complement(1130..1576)
                     /locus_tag="V8Z64_RS00010"
                     /old_locus_tag="V8Z64_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_024235834.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            1748..3298
                     /locus_tag="V8Z64_RS00015"
                     /old_locus_tag="V8Z64_25195"
     CDS             1748..3298
                     /locus_tag="V8Z64_RS00015"
                     /old_locus_tag="V8Z64_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_039063720.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            complement(3494..4618)
                     /locus_tag="V8Z64_RS00020"
                     /old_locus_tag="V8Z64_25200"
     CDS             complement(3494..4618)
                     /locus_tag="V8Z64_RS00020"
                     /old_locus_tag="V8Z64_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252660759.1"
                     /translation="MLEIKMGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDID
                     DSMTVGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAE
                     YSIAQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISP
                     YLDRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKK
                     NASPLITKNFPGFYERVPPCENADECIKKTNIYFDDNFLNIYKRSQDRAPQIISDLKKH
                     NIEIGEKLNDCRKKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLL
                     QYAYINYSKNQIPRDFVTSIKNSIYYKIEDCTKF"
                     /name="hypothetical protein"
     gene            4652..4918
                     /locus_tag="V8Z64_RS00025"
     CDS             4652..4918
                     /locus_tag="V8Z64_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033869189.1"
                     /translation="MESIHTDKPTYYVCAQRLTQFEKISHQFTFFWMRKFAVLTCSLFK
                     TSETGHESLSCIVMQPPENNSGAFFWSGLLFPCLFAFFATESD"
                     /name="hypothetical protein"
     Typing          2..936
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1749..3295
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacctc accgtcccta tcacacggat
       61 gagctcgctt tcggtcttcg tatctccggc aaagggcgtg cgctgcttgc tcgctacatc
      121 cagcaaaatc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgtaggagct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgtaaa aaatcccgag
      241 aacgggcacg ctcacctgct ctacgcgctc aatcttgccg tgagaaccgc gcctgatgcg
      301 tcggttaagg cgctgaaata cgccgctgca attgaacgtg cgctgtgtga gaaacttgac
      361 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggcaggtg
      421 atggagtggc gcgaggaagc ctacgccctc gatgaactgg ctgattatct cgatttgaga
      481 gcctcagagc gtcgtagcgt cgataaacat tacgggctgg ggcgaaactg ccatctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcggcagg gctggccagc attctcacag
      601 tggcttgatg ctgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tctccgcctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa gtttcgcaca gtatgtggct gatacgcaca caccagaaat acaggccaag
      781 agaggccgga aaggtggcat cgctaaaggc gaagcctacg atgacaagcg tttcatggcg
      841 ctatgtatgc tggagaatgg atattctcag aaagctattg cggcgatgct gaatgtacat
      901 cgtaatacga tccgtaattg ggcaatgcac aaatagccta tatcagataa cagcgccttt
      961 tcggcgtctt tttgagcagc gggctttccg gcggttttct cctttcagcc cagcaatggt
     1021 gcggcttacc gtatgattga ggtggtcgtg ctcgccgcag tctcatgacc gagcgtagcg
     1081 agcgaatgag cgaggaagca ccagggaact ggaacgtgaa cggaggctct tacgcactgt
     1141 ctttggtgag tagctctttc ccgcgttttt cccattcctg ccattttgga tggttgtgcg
     1201 gtagctctgc gattcctgcg agtgcgccta ccagttcggc agcactaaac tttggctttc
     1261 cggttttgct gtccaccagt cctgccacaa tcatgaggcc tgcggccttg tagagttcat
     1321 gatcccgcgc ctttcgttcc gcccttgcgg ctgccttccg ttctgccgct atgattgccg
     1381 ttactctggc ttttgctgcg gctgctttct ctgcggtttt ttctgcccgt atcagtgcgg
     1441 acaacgtttt ttcatccgtc gttgttcggt tttttttctc tgccagtaac acaagtaatc
     1501 tctgctgctc tgatggtgat ttcagtccgt tgatgtaggc aatttttgca cttaaccact
     1561 cttcacgatt tgtcattctt acctctgcac ttttgccgtt aatgtgagtt gttattatgc
     1621 ataatatcac cttgtttgcc tgcgtgtgag accgtaaaat catccatgat taattcaggg
     1681 atgcgcactt agtagtttct ctccttcgtc gagaaactgt gcgctcctgc ggagggctaa
     1741 aaggcagatg gcaagctacc attgcactgt caaagctgga gcaaaaggca gtgccttaaa
     1801 acatgctgat tacatttcca gatccggcga atataagagt tacaaatcgc gggaggatct
     1861 ggaattttcg tcatccggta acatgccatc atgggcgaag aaaaatcccg cagagctatg
     1921 gaaagccgca gatgaatttg aacgaaaaaa cgggactgca taccgtgaaa ttgagatcgc
     1981 gttaccgcgt gaactgacac gcgaacaacg gatagaactt gttgaagatt tcgtgcaaaa
     2041 ggaacttggc gatcgccatg cctaccagta cgcgatccac aatccgccag gtgcaatcga
     2101 cggaaaggaa cagccacacg cgcatatcat gttttgcgag cggattaatg atggtattga
     2161 acgcgatcca cagcaatttt ttaaacgtgc caacagcaaa tcaccagagc ggggaggcgc
     2221 aaaaaaagcg agtattcccc agacagccgg agagcgaaaa gccgcccttg ttgcgctgcg
     2281 ttcccgctgg gcagatgtgc agaatgagca ccttgcgcga cacggtcacg agtcgcgcgt
     2341 agatcatcgt tctctgaaag aacagggtat caatcgcacg ccggaagttc atcttggccc
     2401 cgttcaggcc gccagtctga atggggagca gattgttgca atccaggaac ggaggaatgc
     2461 cgagcgggaa ctcaagaccg ccagagatgc cgcaaatgcc attcagcagg aacaggagca
     2521 aaagcaaaaa atcaaggcag ttgagcctgt tcgttcagcc cgttcaccgg agttattgct
     2581 tcagtaccgc aaggttatga aaacggtcat tcagggagag gcaaggcttg ctcgtttagg
     2641 ggatgccaat cccaatgcgc tcaaagaaca taagctgctt cagaatgcga aagctaaaaa
     2701 agactctctt tccgaatgga gtagacgaat atatgaaggg gcacggtatt tagacaaact
     2761 ggggcgtaat gttgtttccg cacaaaggga gctgcgggag ctacaggaac aacgcaatgc
     2821 tttgaatggg attcgtggcc tgtttcgggg ggctgacaaa agggagattg atgcgcgtat
     2881 tctggagcaa aaaagtgtgc tggaaaccgc tgaacatgaa aggaatgaat tcaggaacaa
     2941 gctacagcaa gccgaatccg agtgggacaa agaaaacgct gcttttaagc gtaccgaggg
     3001 atataagtac gttggggagc tggacaggta cagagaacgc gagattctgg cggcggcatc
     3061 tttagagaac accaggcaaa aggttgctga agaggtatcc gttgcccgta gccagatgtt
     3121 atctctggag cctgaactca gtatatctgg agatgaaaaa gctcaaatgc gacatgagtt
     3181 actggcagaa atggcgcagg aacggcagca acaggaggaa aaagcactgt gtatacagcg
     3241 atcctgggcc aggggggtat cacgtagtaa cgaacgtgat caagatatgg agcgttgaaa
     3301 atgtagcaat atcgggactg cgccccgaac cccgccaacg tttcacggaa cgcgaaaaaa
     3361 ttccgcgtcc cgtgaaacgt tttctgaatg agaaaaattg tcgttttgat ttactcacag
     3421 ttcagattaa tgaagttaac aggtgttaaa tatttgcagg tggttatatg tgtaaccacc
     3481 tgtaagggaa ggactaaaac ttagtgcaat cttctatttt ataataaata ctatttttta
     3541 ttgatgttac aaagtcccga ggtatttgat ttttgctgta gttaatgtat gcatattgca
     3601 atagttgttt atgaaagttt tcagctctat tctttccctg aaaattatat gtcaacaccc
     3661 catctaaata atcaactcct ccggtatctg ttacaaacca gtaataggtt aatttgacat
     3721 ctgagatatt tagttttttc ctacaatcat ttaacttttc acctatttct atattgtgtt
     3781 tttttaaatc agaaattatt tgaggtgctc tatcttggct tcttttgtag atatttagaa
     3841 agttatcatc aaagtatata tttgtttttt ttatgcattc atctgcgttc tcacaggggg
     3901 ggacacgttc gtagaaaccg gggaagtttt tagttattaa tggtgatgca tttttcttaa
     3961 acgattgata tactttatat atatgctttt cgcatgattc ttttgcatac tcttcactta
     4021 catcacgcca actgtaacta tcggcagatt gaatgtattc gtacccacca caaatttcat
     4081 ttagcgcatt gtcattatgg tcctttagat atagcattct gtctaaataa gggctaattg
     4141 tgagtttgct atatatcatt ggatcttcat taaaagcact gtttagcttt cttatatgat
     4201 ctattatgac ctcttttgtt ttttccacca gaggcgtttt ctgtttgtat tcctctagtg
     4261 attcattggt tgattttatg ctgttgttat tttgtgctat agaatattca gccttatctt
     4321 caggttcttg cataatggct gaatttatta tttttaggcc actttcacta atatcacagg
     4381 tatatgtgac gatggttctt cctcgatcat cctctttttc actccatgtg ccattgatgc
     4441 aatcactcct tgtatcgaag gctttaccaa cagtcattga gtcgtcaatg tcgtaatata
     4501 cgaaagactt taccttgctt attccatcag agtcgcatcc tgaaataaaa aatgacgaca
     4561 ataaaggaag gatatgttta aagttaatat tcatttttcc catttttatt tctaacaata
     4621 acgaggaaat attaatacag gcgatatgtt aatggaatcc atacataccg ataaaccgac
     4681 ttactatgtt tgtgctcaac gtcttacgca gttcgaaaag atttcgcacc agttcacctt
     4741 tttctggatg agaaagtttg ctgttttgac ctgttcgctg ttcaaaacga gtgaaaccgg
     4801 acatgaatcg ctttcgtgca tagttatgca gccccctgaa aacaattctg gcgcgttttt
     4861 ttggtctggc ctgttgtttc cctgtctttt tgcgtttttt gcgacagaaa gcgactgagg
     4921 gcgttttaag gggggcgcac aacgggagtt atggtaaatg gatcgggttt gcgggaagac
     4981 tcgacaggtt ttgtcgttgg gggtagtgta agcgactgaa aaacaaacgc ccctgaaatt
     5041 cgtgtctatc cggcaagact ttcacgaaga tacaggggcg atgtcggtcg ataacctgga
     5101 aggaatcgta acac
//
