LOCUS       NZ_CP152300             6015 bp    DNA     circular CON 20-MAY-2024
DEFINITION  Shigella sonnei strain R16.0823 plasmid pR16.0823_6k, complete
            sequence.
ACCESSION   NZ_CP152300
VERSION     NZ_CP152300.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123227
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: flye v. v2.9.2
            Genome Representation        :: Full
            Expected Final Version       :: No
            Genome Coverage              :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_039684325.1-RS_2024_05_20
            Annotation Date              :: 05/20/2024 11:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R16.0823"
                     /db_xref="taxon:624"
                     /plasmid="pR16.0823_6k"
                     /collected_by="Taiwan CDC"
     gene            1..936
                     /locus_tag="V8Z64_RS00005"
                     /old_locus_tag="V8Z64_25130"
     CDS             1..936
                     /locus_tag="V8Z64_RS00005"
                     /old_locus_tag="V8Z64_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271369.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSTSA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(1130..1528)
                     /locus_tag="V8Z64_RS00010"
                     /old_locus_tag="V8Z64_25135"
     CDS             complement(1130..1528)
                     /locus_tag="V8Z64_RS00010"
                     /old_locus_tag="V8Z64_25135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1704..2630
                     /locus_tag="V8Z64_RS00015"
                     /old_locus_tag="V8Z64_25140"
     CDS             1704..2630
                     /locus_tag="V8Z64_RS00015"
                     /old_locus_tag="V8Z64_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2633..3166
                     /locus_tag="V8Z64_RS00020"
                     /old_locus_tag="V8Z64_25145"
     CDS             2633..3166
                     /locus_tag="V8Z64_RS00020"
                     /old_locus_tag="V8Z64_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021546188.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            3387..5024
                     /locus_tag="V8Z64_RS00025"
                     /old_locus_tag="V8Z64_25150"
     CDS             3387..5024
                     /locus_tag="V8Z64_RS00025"
                     /old_locus_tag="V8Z64_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_039063724.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVSV
                     TDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKGTKDYGHDY
                     HPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHGELEGYRASD
                     GQHLGSFDPKTGKQLKGPDPKRSIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5034..5291
                     /locus_tag="V8Z64_RS00030"
                     /old_locus_tag="V8Z64_25155"
     CDS             5034..5291
                     /locus_tag="V8Z64_RS00030"
                     /old_locus_tag="V8Z64_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_001419702.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
                     /name="cloacin immunity family protein"
     gene            5375..5518
                     /locus_tag="V8Z64_RS00035"
                     /old_locus_tag="V8Z64_25160"
     CDS             5375..5518
                     /locus_tag="V8Z64_RS00035"
                     /old_locus_tag="V8Z64_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001419703.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6015
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..936
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.338"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1705..2627
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc tccgccccta tcacacggat
       61 gatctcgctt tcggccttcg tatctccggt aaagggcgcg cgcttcttgc acggtatatc
      121 cagcagaacc aacctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgacct gaacgccccc gcaccgaaca tcaccataaa aaatcccgtg
      241 aacggtcacg cccacctgct ctacgcactc aatatcgccg tgagaaccgc gcctgatgct
      301 tcggttaagg cgctgaaata cgccgccgcg attgaacgtg cgttgtgtga aaaactgggc
      361 gcagatgtaa actacagcgg actgatttgc aaaaatccgt tccatctgga atggcaggtg
      421 atggagtggc gtgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 acttcagcgc gccgtagcat cgataaacat tacggaatgg ggagaaactg ccatctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tcacctcctg aatgtcgggc tattggcaag agtattgcga aatacacgca caggaacttc
      721 acggcggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat acaggccaag
      781 agaggcagga aaggtggcat cgctaaaggc gaagcctacg atgacaagcg tttcatggcg
      841 ctatgtatgc tggagaatgg atattctcag aaagctattg cggcgatgtt ggaggtttct
      901 actcgaacca ttcgaaactg gaaaagcgga aaatagccta tatcagataa cagcgccttt
      961 ctggcgtttt tttgagcctt aggtcttttg ccgtttttcc ccgttcagcc ctgcaatggt
     1021 gctgttttcc gcatgattgt gctggtagtg ctcgccgcag tctcatgacc gagcgtagcg
     1081 agcgaatgag cgaggaagca ccagggaaca ggaacacgga tggaggcact tacgcgtctg
     1141 ggtttgggga tgtctccaga tcgtacttaa tttccttaag tgcctgtaat atcctgtctt
     1201 ttttatagca tagctcgcct gtttttgtgt ctaccagtcc tgcttcgatc agtagcccag
     1261 ccgcgagaaa gagtcggtga ttgcgttctc gtcgttgttt ttttccctcg attcgttttt
     1321 ggaccgcctg ttttcgttcg ctctcctgtg cctgtaagtc gtttttttcc gctgttgcca
     1381 gtacgtggag gagtttgcgt tcctgttctg tgagtcctgc ttcgttgtcg agcaggtcaa
     1441 taatctgtct ctgtcgtgcg gtctgttctc cgagcttcct gatgaatccg atccgtttct
     1501 tcagccattc gatgttgctg atgcgcatgt tcgtggtgct ccctggtgtt ttttgctgtg
     1561 tgttgtggtc gtgcagtata tcgcttgcgg tcggtgggtg cagggggtat cttccgtgct
     1621 aattcgtaaa attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg
     1681 cgctcctgcg gagggctgaa accgtggcaa cctatcacct cagcgttaaa tttggtggca
     1741 aggggcaggc tgcaaaccac gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc
     1801 aggatttgga atattccgca cacggaaaca tgccggaatg ggcgcgagat aatccctcac
     1861 atttctggca ggcggctgac cagtttgaac gtgcgaacgg ttcgacctat cgggaacttg
     1921 aaattgcatt accccgtgaa ctgacagcgg aacagcgcct tgaactggtg caggattttg
     1981 tacgtcagga agctggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga
     2041 gcattgacgg cggcgaacag cctcatgcgc atatcatgat gagccagcgt gtgaatgatg
     2101 gcattgaacg tacaccggaa cagtatttcc ggcgctataa cgccaggtat cccgagcggg
     2161 gaggtgcgaa aaaggattcc ggaagcctga ctctcactca gcaaaaagag cagttgcggg
     2221 aattgcgaaa acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa
     2281 ggggcttcat tgactgccgg acgctgaaag aacagggcat tgaacgacgt cccgaagtgc
     2341 atctgggttt tgaagctgcc ggacggcttg atgatgccca gcgtcatgac atcatgcaaa
     2401 aacggtctga accggactat tcccgccatc ttgaacaggc gcgaacggtc gggcatgaca
     2461 tcagggaatg gctggacact ccggttaagc aacatgaact ggtcagaagg ctgcaacatg
     2521 atgttgcgcg ggagcatggc tttgacatga gagacagagt gagcagacag aaccgaactg
     2581 aacctgaacg ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag
     2641 cacagaagaa aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat
     2701 ggagcaacag aaagctgttg ctgattctct ggctgtggca agggaaacac ttcgcggata
     2761 tcgggatatt tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa acgaagtgca
     2821 gaggctggat gttgcagggc tgataggtga gcgcatcagt caggattttc agcggctgga
     2881 agagtcagtc gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat
     2941 tcacagcaat atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg
     3001 tggggtgtca gtggcctttc tggttatgtt cagtgtgctc ggatggggga tgaaaacaat
     3061 tctcgacagg aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga
     3121 taaactggtg gagaaacctg tcacgggtaa gagccggaaa aactgacgcg cttcgcttgt
     3181 ccaacgcctc acgacccgcg aaaggatttc gcgttccgtt caccgttttc atgagtgatt
     3241 cctccgaact gccaaaaaat atccccccct gacttgacag aaaaaataat ggcgagtact
     3301 ttttggtctg tacataaaac cagtggtttt atgtacagta tcaactatat aatcaattgt
     3361 tttaacgctt aaaagaggga atttttatga gcggtggaga tggacgcgac cacaacagtg
     3421 gcgcacataa cacaggtggt aacattaatg gcggccctac ggggcttggt ggaaatggtg
     3481 gggcttctga cggttcagga tggagttcgg aaaataaccc atggggtggc ggttccggta
     3541 gtggtgttca ctggggaggt ggctccggca atggtaatgg cggggggaat ggtaattccg
     3601 gtggtggcag caattcatcc gtagcagcag tggcatttgg ttttcctgct ttggcaactc
     3661 ctggtgccgg aacgctgggt atcgccgtct ctggtgaggc tttatctgcg gcaatagcgg
     3721 atatctttgc agccctgaaa ggcccgttta aattcagcgc atggggtatt gcgctttacg
     3781 gcattctgcc atctgaaata gcaaaagatg acccgaatat gatgtcaaag attgtgacgt
     3841 cattaccggc agaaacagtg acgaatgttc aggtcagtac acttccgctg gaccaggcaa
     3901 ctgtcagcgt gacgaaacgt gtgacagatg ttgtgaagga cacacgacag catattgccg
     3961 tagttgcagg tgtgcctatg agtgtgcctg ttgtgaatgc caaaccaaca cgtactccag
     4021 gtgtatttca cgcatcattt cctggtgtcc cttctctgac agtcagcact gtcaaaggcc
     4081 ttccggtgtc aacaaccctt ccccgtggta ttacggagga taaaggccgg actgccgttc
     4141 ctgcgggatt tacctttggt ggtggttcac atgaagcggt gattcgcttt ccgaaagaaa
     4201 gtgggcagaa gccggtttat gtatcagtga cagatgttct taccccagca caggtaaaac
     4261 aacgtcagga tgaggaaaaa cgcctccagc aggaatggaa tgacgcacat ccggtggaag
     4321 tggctgaacg caattatgaa caggcccgcg cagaactgaa tcaggctaat aaagatgttg
     4381 ccagaaacca ggaacgacag gctaaagctg ttcaggttta taattctcgt aaaagtgaac
     4441 ttgatgcagc gaataaaact cttgctgatg caaaggctga aataaaacaa ttcgagcgat
     4501 ttgcccgaga accaatggct gctggtcaca gaatgtggca aatggcaggg cttaaggccc
     4561 agcgggcaca gacggatgta aataataaga aggctgcatt tgatgctgcc gcaaaagaga
     4621 agtcagcagc agatgctgca ttgagttctg cgatggaaag caggaagaag aaagaagata
     4681 agaaaaggag tgctgaaaat aaattaaacg aagagaaaaa taaacgccga aaaggcacta
     4741 aagattatgg ccatgattat catccagctc ctaaaactga ggatattaaa ggtttggggg
     4801 aattaaaaga ggggaggcca aaaactccaa agcaaggtgg tggaggtaag cgtgccagat
     4861 ggtatggaga taaggggcgt aaaatttatg agtgggactc tcagcatggt gagcttgagg
     4921 gctatcgagc cagtgatggt cagcatcttg gttcatttga ccctaaaacc ggtaagcagt
     4981 tgaaaggtcc agatccgaaa cgaagtatta aaaagtatct ttaaggtgat gttatgggac
     5041 ttaaattaca cattcattgg tttgataaga agaatgagga atttaaaggt ggtgagtatt
     5101 caaaggattt cggtgatgat ggttcggtta ttgaacgtct tggaatgcct ttaaaggata
     5161 atattaataa cggttggttt gatgttgaaa agagatgggt gccaatatta cagccatact
     5221 ttaaaaatgt tattgatatt agtaagttcg attactttgt ttctttcgat tatcgtgatg
     5281 gtgactggta attaaactat cagggacgag taataaagtg tttgattcag gtgttcaagg
     5341 atgaattcct gaatccatat atacagggaa tcgtatgaag aaaataacag ggattatttt
     5401 gttgcttctt gcagccatta ttcttgctgc atgtcaggca aactatatcc gtgatgttca
     5461 gggcgggact gtatcaccgt catcctctgc tgaactgacc ggagtggaaa cgcagtaacc
     5521 tgcaatcctc atttgacaag gacaaagcgt gtcaggctga ttctgacgcg ctttttttgt
     5581 tgaagtgcca caaaaattcc atgtgggaga tgggatctaa aatctccgtg cagaaccttc
     5641 catccggagg gagaaaactt ttcgttttga gccgttcggt gttcagaacg cacgaaaccg
     5701 atcgcacatc gctttcgtgc atagttatgc aacgccctga aaacgattct gacgcgtttt
     5761 tctggtttgt cctggtgttt tcttgtcttt ttgcgttttt tgcgccagaa agcgattgag
     5821 ggcgttttaa ggggtacgta caacgggagt tatggtaaat ggatcgggtt ttcgggaagg
     5881 atcgacagga tttgccgttg ggtgtagtgt aagcgactga aaaacaaacg cccctgaaat
     5941 tcgtgtctct ccgccaagat tgtcacgaga atacaggggc gaggtcggtc gataacctgg
     6001 aaggaatcgt aacac
//
