LOCUS       NZ_CP152092            12378 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Enterococcus faecium strain EF9953 plasmid pEF9953.4, complete
            sequence.
ACCESSION   NZ_CP152092
VERSION     NZ_CP152092.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41030363
            Assembly: GCF_038665115.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Wei,J.
  TITLE     Complete Genome Sequence of Enterococcus faecium strain EF9953
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12378)
  AUTHORS   Wei,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2024) Zhuhai People's Hospital, Jinan University,
            79 Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; FALCON v. 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.43x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038665115.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 23:48:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,307
            CDSs (total)                      :: 3,212
            Genes (coding)                    :: 3,049
            CDSs (with protein)               :: 3,049
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 50 of 163
            Pseudo Genes (incomplete)         :: 109 of 163
            Pseudo Genes (internal stop)      :: 36 of 163
            Pseudo Genes (multiple problems)  :: 29 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152092.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="EF9953"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEF9953.4"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2022-02"
                     /collected_by="Jie Wei"
     gene            complement(join(12348..12378,1..122))
                     /locus_tag="AAGS98_RS16435"
                     /old_locus_tag="AAGS98_16435"
     CDS             complement(join(12348..12378,1..122))
                     /locus_tag="AAGS98_RS16435"
                     /old_locus_tag="AAGS98_16435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_342606828.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKRQFV
                     LRLTN"
                     /name="RepB family plasmid replication initiator protein"
     gene            759..845
                     /locus_tag="AAGS98_RS16440"
                     /old_locus_tag="AAGS98_16440"
     CDS             759..845
                     /locus_tag="AAGS98_RS16440"
                     /old_locus_tag="AAGS98_16440"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(966..1655)
                     /locus_tag="AAGS98_RS16445"
                     /old_locus_tag="AAGS98_16445"
     CDS             complement(966..1655)
                     /locus_tag="AAGS98_RS16445"
                     /old_locus_tag="AAGS98_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1662..1940)
                     /locus_tag="AAGS98_RS16450"
                     /old_locus_tag="AAGS98_16450"
     CDS             complement(1662..1940)
                     /locus_tag="AAGS98_RS16450"
                     /old_locus_tag="AAGS98_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(1900..2814)
                     /locus_tag="AAGS98_RS16455"
                     /old_locus_tag="AAGS98_16455"
     CDS             complement(1900..2814)
                     /locus_tag="AAGS98_RS16455"
                     /old_locus_tag="AAGS98_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2796..3176)
                     /locus_tag="AAGS98_RS16460"
                     /old_locus_tag="AAGS98_16460"
     CDS             complement(2796..3176)
                     /locus_tag="AAGS98_RS16460"
                     /old_locus_tag="AAGS98_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3626..3913)
                     /locus_tag="AAGS98_RS16465"
                     /old_locus_tag="AAGS98_16465"
     CDS             complement(3626..3913)
                     /locus_tag="AAGS98_RS16465"
                     /old_locus_tag="AAGS98_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            complement(3933..4157)
                     /locus_tag="AAGS98_RS16470"
                     /old_locus_tag="AAGS98_16470"
     CDS             complement(3933..4157)
                     /locus_tag="AAGS98_RS16470"
                     /old_locus_tag="AAGS98_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            complement(4627..4797)
                     /locus_tag="AAGS98_RS16475"
                     /old_locus_tag="AAGS98_16475"
     CDS             complement(4627..4797)
                     /locus_tag="AAGS98_RS16475"
                     /old_locus_tag="AAGS98_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            complement(4812..5345)
                     /locus_tag="AAGS98_RS16480"
                     /old_locus_tag="AAGS98_16480"
     CDS             complement(4812..5345)
                     /locus_tag="AAGS98_RS16480"
                     /old_locus_tag="AAGS98_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(5366..6310)
                     /locus_tag="AAGS98_RS16485"
                     /old_locus_tag="AAGS98_16485"
     CDS             complement(5366..6310)
                     /locus_tag="AAGS98_RS16485"
                     /old_locus_tag="AAGS98_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            6947..7033
                     /locus_tag="AAGS98_RS16490"
                     /old_locus_tag="AAGS98_16490"
     CDS             6947..7033
                     /locus_tag="AAGS98_RS16490"
                     /old_locus_tag="AAGS98_16490"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(7154..7843)
                     /locus_tag="AAGS98_RS16495"
                     /old_locus_tag="AAGS98_16495"
     CDS             complement(7154..7843)
                     /locus_tag="AAGS98_RS16495"
                     /old_locus_tag="AAGS98_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(7850..8128)
                     /locus_tag="AAGS98_RS16500"
                     /old_locus_tag="AAGS98_16500"
     CDS             complement(7850..8128)
                     /locus_tag="AAGS98_RS16500"
                     /old_locus_tag="AAGS98_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(8088..9002)
                     /locus_tag="AAGS98_RS16505"
                     /old_locus_tag="AAGS98_16505"
     CDS             complement(8088..9002)
                     /locus_tag="AAGS98_RS16505"
                     /old_locus_tag="AAGS98_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8984..9364)
                     /locus_tag="AAGS98_RS16510"
                     /old_locus_tag="AAGS98_16510"
     CDS             complement(8984..9364)
                     /locus_tag="AAGS98_RS16510"
                     /old_locus_tag="AAGS98_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(9814..10101)
                     /locus_tag="AAGS98_RS16515"
                     /old_locus_tag="AAGS98_16515"
     CDS             complement(9814..10101)
                     /locus_tag="AAGS98_RS16515"
                     /old_locus_tag="AAGS98_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            complement(10121..10345)
                     /locus_tag="AAGS98_RS16520"
                     /old_locus_tag="AAGS98_16520"
     CDS             complement(10121..10345)
                     /locus_tag="AAGS98_RS16520"
                     /old_locus_tag="AAGS98_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            complement(10815..10985)
                     /locus_tag="AAGS98_RS16525"
                     /old_locus_tag="AAGS98_16525"
     CDS             complement(10815..10985)
                     /locus_tag="AAGS98_RS16525"
                     /old_locus_tag="AAGS98_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            complement(11000..11533)
                     /locus_tag="AAGS98_RS16530"
                     /old_locus_tag="AAGS98_16530"
     CDS             complement(11000..11533)
                     /locus_tag="AAGS98_RS16530"
                     /old_locus_tag="AAGS98_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(11554..12225)
                     /locus_tag="AAGS98_RS16535"
                     /old_locus_tag="AAGS98_16535"
     CDS             complement(11554..12225)
                     /locus_tag="AAGS98_RS16535"
                     /old_locus_tag="AAGS98_16535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_342606827.1"
                     /translation="MFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELT
                     AIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKE
                     YEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLF
                     Q"
                     /name="RepB family plasmid replication initiator protein"
     BGC             1..12378
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5367..6310)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11555..12378)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1904..2814)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8092..9002)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttatcccctt tttcacgcat tctagacacg atggaaaaaa acaaattcat ttcaaccggt
       61 gtaaattttc ttaaaggaat tgtatttaat tcatggtggt attttacaat ctcattagcc
      121 atataatcac ctcaatccaa ttatgaatga cattttttaa atgtcaagac attcatattt
      181 gacgttcaaa cagacaaaat gtccttcata aacagacaaa atgtccttca taaacagaca
      241 aaatgtcctt cataaacaga caaaatgtcc ttcataacat tctgtatcca ttggtagagt
      301 aaggacacag cttacggtaa agaggtttaa aaagaggttt aaaaagagat ataaaaagag
      361 gggggctaat tttcccgcct aaaaaccact tcttttttct ttaaacaggt tcgcgcattt
      421 ttcaaaactg ccaatccaaa accttgcttc ctacggtcgc caaaaaggtg tgatcgctac
      481 gctctcacat tgtactgcgc agtcgcctaa aggctccttt gctaaaaaca tgttggtaaa
      541 gaggcctttg agcggttctg aatgcttatc taagccgttt taagacagtc ttggataaaa
      601 atccttatct agctttaaag cgccttacag gcggtcaggg caagccctaa caaccctaaa
      661 ataagcgata cttaaaaaca ccacacaagt ttaatttttt aagatataat aacttgtcgc
      721 ctatccgaca gtcttgcgac tggaaggagg tgtatctaat gtttgaattg ttcatttgcc
      781 cccttcttat agaagtttgt gtctctttat tcagttattg gttgaataac cgaaacaaga
      841 agtaaaatca ataaaggcga catcaacctg aaaaaaagcc ccagagaatc cgacctctct
      901 ggggctttcg tgtatctaat gtttgatcat ttgcatgttt agtttaacat gattgccgat
      961 caaattcaat tttgctgttg ctgatctaac cattccttga cactttttag ataatttaat
     1021 gccgtttctt ttttcgttgg attttgtatg ttcgttgctt gctgaagcag tgctttataa
     1081 cgttcgtagg taactgattg ttgccgttct tttcgttcct gttcactagt tgaaaaaggg
     1141 attgtatcac tgaataaatt ataacaagct tcttctgtct gcgtatcttt aaaatattgc
     1201 acgtcttttt tggttcgctc atagttgtct tgaacctctt tttgctgttt ttctggcaag
     1261 ttttgataaa gacgagtgag tttttccatg tttttatccg tcaaacgttt ttcttgattc
     1321 acaaaaatat cgttcatttc ctcataacaa gtggtaaact cttccatttt ttgtgttaac
     1381 acttgctcct ttttcttttt atcacatgct gcttcatact tttgagtgat atcccctaat
     1441 aaaatacttc ttgaaatacg tttatttccc tctaaaacgc cgaataaacc acttaaaacg
     1501 attgataatg taaatataac ccctggcaat aaaaacctct taaatcgaat tttagagcct
     1561 tttaatgaat atcttgtttg ataagcctta tagctaagcc aaataattcc taaaatactg
     1621 gcaaaaatga gtagataggg taaatagaat gccatttcta tttattctcc ccataccagc
     1681 caaaaggcta acagtgtcat gattaagcaa cataaggggg caagaatatc cattgatcgc
     1741 tgcataatta cttttttctg ttctttattt cgctggttta atagcgcatg gtggaatttt
     1801 ttgacttgtg aatcaattgt agtttgtatt ctttctttgg tataaggtgc aagaaaagca
     1861 cgttgtttct ttatttcatt ttctatgtct tgtaacgttt tagaacgata attcttgttg
     1921 gtagtctgct cctctttcaa cgtgttggtc atggtttctt cgagtcggtc taatttccgg
     1981 ttgttcagtc gtaagagttc gttgttctct tcgtacttct tttgcagttt cgcgaatgct
     2041 agctcgagct cttcgtagct cgttggttcg ttgttgattg taggctcgtt ctcgtctgtc
     2101 cagctcatgt aaaatcgtct ccttttcgta atcctttcct agtttgttgg atcgtacttt
     2161 tttctttttg tggatcaatt cgtaagtaaa atttttccca cgattcaagc aatgaatgcc
     2221 gaatgtttta agagaatcac aaaattggtt atacgttcga gtttttttgt ttttcattgt
     2281 ttgatcaatc gcaaaccgaa tttcgtcttt ccaaacaggt tcattttttt cttttaattt
     2341 gatttcagac atggttcgtt tttcgggttt cttccctcgt tcagtgattg ttaagtcatg
     2401 ttcaagacaa atctcgtcat ttaggtcata acatttgttt aaaaatgctt gaccatgggc
     2461 ttgaaatttg ttcccagttt ccagattgac ggagttaatg acaatgtggt tatgaatatg
     2521 gtctttgtct gtatgtgtgt aaatcgcgat ttggtggtca ggggctattt ttttcgcata
     2581 ctctgcccct agttgattgg ctttttcagc ggtaatttct cctggtcgaa aagactggat
     2641 caataagtgt gcttgtacac gatcgtcttt tccatacagc atgcgaattt gtttcatttc
     2701 gcttttggca taatcaatgt cacagtttat ccctgttttg acggttgctc gtggttcggc
     2761 gtaattgatc gctcggctac aagaagtggt tcggcttact ttaactgttg ccatatcttt
     2821 tgtagctcct tttgcatgat ttttaggttt tcggataagc gttgggcgtc gccaggatca
     2881 acgtcgtgtt ggtgtgtgtt gcaccacttg gcaatttgat tgatattccc gccagctttg
     2941 gctaaatcgc gcgcgattgc ctgagcaatg tctggcgcca ctttcggtgt caccagacgc
     3001 gcgttttgtg ccttggcttt cacgaaagcc ggcacactca tttgcaaagc ttcagctgac
     3061 tgcttcagct ttgcaaattc ggattcgctc actcgaaaac tgatttgttt tggctcggat
     3121 cgataggtat attttttaga atgattgcta gccaaatatt gattttgttc gctcataagt
     3181 cccacccttt cagtacgtcc tacggacgta tcgttcgcaa taccattgta cctaaaaagg
     3241 tacaataagt aaagctcact aaagctcact aaagggcttg gcaagctcta tatttgctag
     3301 ccaaatacct acgtatttgg tgcaaataac cgcttgtcaa aggggcgcaa gctccccctt
     3361 gaatccccct cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt
     3421 ttgtaaagtt tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga
     3481 gctacatttt tttcgttgtt caaagattct tatgccattt actattaata aatctataaa
     3541 tatgataatt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt
     3601 cccattttca ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat
     3661 atacttgaga ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa
     3721 atttatgttt taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa
     3781 cactttttcc ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat
     3841 agtcatctat agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt
     3901 tagtaaaatc cattataata acctccctac cactaccttc taggtgccca tggaccatga
     3961 ttaacccaac cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc
     4021 caacattttt ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt
     4081 attcctgtac cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt
     4141 aatactttct ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt
     4201 aaataatctt agtaatttta ttatataaca ttttattata taacaattta ttatataaca
     4261 atgattaacg tcataaaaag tagttttgaa ttcataatcg tttaaataaa attataagat
     4321 cagttattta aacagctagt agacataatg tgtatactac caagtaacgc ctgtaccaag
     4381 ggatccaggc gctcactttg tgattttcga ttggtctaag tcaaaagctc tttttaaggc
     4441 ttggttttct gcggttgttt ctttctaatt ctttgtgaat attgatcggt cattttttaa
     4501 atacttgtta tttttgtttg tgtttgttat gctttttaca tatctcatat tcatttatta
     4561 ttcttttcta aggttaataa taggataatc tctttcagaa agactgctcg tacgagcagt
     4621 cttttcttat ttatcaagtt tttctacaaa tacttcctca ttttgactgt tgtagtaatt
     4681 aggattgagt tttagttccc cttttttatt tcctttgttg gccactgata ataacattgg
     4741 gctaaagctt aatattccta gtaggataga accgaatagc aagtgttttt ttgtcataag
     4801 tatttccttc ttcatttcca tagctgccac cattttttcc aattctttgt ttgttcgtcc
     4861 attgtttctg ttgcttcaga aagagtcgtt gaactattct ctgaagactc ttccgtataa
     4921 gtcaactgca gttgctcctg aagtttttcg atttgttggt tggcttgcag ggtgagttgt
     4981 tgttgctggt cgagtagttt ttgtatatga gaattatttt gctctttgag ggcaagttgt
     5041 tctcgtaaaa attcattttc ttcacgcaac tgatctagca acttgtttgc aaccacgttt
     5101 ttgtttggta tataagcatt tttatcgttg ttttgttcat gtttatgcaa cttatctagc
     5161 aacctttgat tgtctaagtt gcgagcaagt tgcaatactc cagaacgcaa tacaacctta
     5221 taaccacgct catttttagc tatcatggaa gaatctaatt tttggatatg tttcgatatt
     5281 gcttgcctac tcatcttaaa aagatctgac aattccttta ttgtaaaggt ttcttgtggt
     5341 tgcatactgg ttgcctccta ggttgctact ggaataaatt atctaaatca gataataaac
     5401 tttttttctc tttttccgaa agtgcggact ctgtttgttt ttcaagagct tttttctctt
     5461 tttctagttg tttgatttgt tggaggagtt caagttctcg tttcttctgt ttgatttttt
     5521 cgttaattgt tgtagcgggt tcttcgtatt ctttttgttt atcagctaaa gccaatgctt
     5581 tattttggtc ttctttggtt ggtgcatgtt tgttaaagtc gtctgctttt ttgatctcag
     5641 gtttgaacgt aaattgatag ccaataacag gttttccccg tccttttccg tatttctttt
     5701 tgattgttag tcctttgaag attgcagtta gttcttgacg tataggtttt ataactcgtt
     5761 tatctatatc aggttgctta taacttttag gaatatctaa taattcaaga aagtcttctt
     5821 tggagaaata tgcgaaacct ttggttctgt attgctttaa cagacgaaac atagtctttg
     5881 catagccact ttgtagctcg ttgaattgtt gtaaagaata gcgtacccac tcgtctaaat
     5941 tatttaacaa aggcagtgct ttttcgtaaa ttcgtatatc tacgtaagga ttttctacgt
     6001 ccccgttgat tttaaattct gtaaacatta caaacatatc gtaagaaagc ccagactcac
     6061 ttctacgacc aaagcgtaaa gataagattt tttcataagt tgcttttaaa tcttctacaa
     6121 atcgtacatt tgcagttgct ttgtagttac ttaaatcttt tagttggtca aacgtaaaac
     6181 gaactgtctt atcccctttt tcacgcattc tagacacgat ggaaaaaaac aaattcattt
     6241 caaccggtgt aaattttctt aaaggaattg tatttaattc atggtggtat tttacaatct
     6301 cattagccat ataatcacct caatccaatt atgaatgaca ttttttaaat gtcaagacat
     6361 tcatatttga cgttcaaaca gacaaaatgt ccttcataaa cagacaaaat gtccttcata
     6421 aacagacaaa atgtccttca taaacagaca aaatgtcctt cataacattc tgtatccatt
     6481 ggtagagtaa ggacacagct tacggtaaag aggtttaaaa agaggtttaa aaagagatat
     6541 aaaaagaggg gggctaattt tcccgcctaa aaaccacttc ttttttcttt aaacaggttc
     6601 gcgcattttt caaaactgcc aatccaaaac cttgcttcct acggtcgcca aaaaggtgtg
     6661 atcgctacgc tctcacattg tactgcgcag tcgcctaaag gctcctttgc taaaaacatg
     6721 ttggtaaaga ggcctttgag cggttctgaa tgcttatcta agccgtttta agacagtctt
     6781 ggataaaaat ccttatctag ctttaaagcg ccttacaggc ggtcagggca agccctaaca
     6841 accctaaaat aagcgatact taaaaacacc acacaagttt aattttttaa gatataataa
     6901 cttgtcgcct atccgacagt cttgcgactg gaaggaggtg tatctaatgt ttgaattgtt
     6961 catttgcccc cttcttatag aagtttgtgt ctctttattc agttattggt tgaataaccg
     7021 aaacaagaag taaaatcaat aaaggcgaca tcaacctgaa aaaaagcccc agagaatccg
     7081 acctctctgg ggctttcgtg tatctaatgt ttgatcattt gcatgtttag tttaacatga
     7141 ttgccgatca aattcaattt tgctgttgct gatctaacca ttccttgaca ctttttagat
     7201 aatttaatgc cgtttctttt ttcgttggat tttgtatgtt cgttgcttgc tgaagcagtg
     7261 ctttataacg ttcgtaggta actgattgtt gccgttcttt tcgttcctgt tcactagttg
     7321 aaaaagggat tgtatcactg aataaattat aacaagcttc ttctgtctgc gtatctttaa
     7381 aatattgcac gtcttttttg gttcgctcat agttgtcttg aacctctttt tgctgttttt
     7441 ctggcaagtt ttgataaaga cgagtgagtt tttccatgtt tttatccgtc aaacgttttt
     7501 cttgattcac aaaaatatcg ttcatttcct cataacaagt ggtaaactct tccatttttt
     7561 gtgttaacac ttgctccttt ttctttttat cacatgctgc ttcatacttt tgagtgatat
     7621 cccctaataa aatacttctt gaaatacgtt tatttccctc taaaacgccg aataaaccac
     7681 ttaaaacgat tgataatgta aatataaccc ctggcaataa aaacctctta aatcgaattt
     7741 tagagccttt taatgaatat cttgtttgat aagccttata gctaagccaa ataattccta
     7801 aaatactggc aaaaatgagt agatagggta aatagaatgc catttctatt tattctcccc
     7861 ataccagcca aaaggctaac agtgtcatga ttaagcaaca taagggggca agaatatcca
     7921 ttgatcgctg cataattact tttttctgtt ctttatttcg ctggtttaat agcgcatggt
     7981 ggaatttttt gacttgtgaa tcaattgtag tttgtattct ttctttggta taaggtgcaa
     8041 gaaaagcacg ttgtttcttt atttcatttt ctatgtcttg taacgtttta gaacgataat
     8101 tcttgttggt agtctgctcc tctttcaacg tgttggtcat ggtttcttcg agtcggtcta
     8161 atttccggtt gttcagtcgt aagagttcgt tgttctcttc gtacttcttt tgcagtttcg
     8221 cgaatgctag ctcgagctct tcgtagctcg ttggttcgtt gttgattgta ggctcgttct
     8281 cgtctgtcca gctcatgtaa aatcgtctcc ttttcgtaat cctttcctag tttgttggat
     8341 cgtacttttt tctttttgtg gatcaattcg taagtaaaat ttttcccacg attcaagcaa
     8401 tgaatgccga atgttttaag agaatcacaa aattggttat acgttcgagt ttttttgttt
     8461 ttcattgttt gatcaatcgc aaaccgaatt tcgtctttcc aaacaggttc atttttttct
     8521 tttaatttga tttcagacat ggttcgtttt tcgggtttct tccctcgttc agtgattgtt
     8581 aagtcatgtt caagacaaat ctcgtcattt aggtcataac atttgtttaa aaatgcttga
     8641 ccatgggctt gaaatttgtt cccagtttcc agattgacgg agttaatgac aatgtggtta
     8701 tgaatatggt ctttgtctgt atgtgtgtaa atcgcgattt ggtggtcagg ggctattttt
     8761 ttcgcatact ctgcccctag ttgattggct ttttcagcgg taatttctcc tggtcgaaaa
     8821 gactggatca ataagtgtgc ttgtacacga tcgtcttttc catacagcat gcgaatttgt
     8881 ttcatttcgc ttttggcata atcaatgtca cagtttatcc ctgttttgac ggttgctcgt
     8941 ggttcggcgt aattgatcgc tcggctacaa gaagtggttc ggcttacttt aactgttgcc
     9001 atatcttttg tagctccttt tgcatgattt ttaggttttc ggataagcgt tgggcgtcgc
     9061 caggatcaac gtcgtgttgg tgtgtgttgc accacttggc aatttgattg atattcccgc
     9121 cagctttggc taaatcgcgc gcgattgcct gagcaatgtc tggcgccact ttcggtgtca
     9181 ccagacgcgc gttttgtgcc ttggctttca cgaaagccgg cacactcatt tgcaaagctt
     9241 cagctgactg cttcagcttt gcaaattcgg attcgctcac tcgaaaactg atttgttttg
     9301 gctcggatcg ataggtatat tttttagaat gattgctagc caaatattga ttttgttcgc
     9361 tcataagtcc caccctttca gtacgtccta cggacgtatc gttcgcaata ccattgtacc
     9421 taaaaaggta caataagtaa agctcactaa agctcactaa agggcttggc aagctctata
     9481 tttgctagcc aaatacctac gtatttggtg caaataaccg cttgtcaaag gggcgcaagc
     9541 tcccccttga atccccctcc cccccactct ccacaataca aaaaagacta gcaaatgcta
     9601 gccacttttt gtaaagtttc gcttgtttca ccttcggtga aattgttgca aatcaataat
     9661 taggtagagc tacatttttt tcgttgttca aagattctta tgccatttac tattaataaa
     9721 tctataaata tgataatttt tcggctctat aatttatggg actgatgaat cagaattgat
     9781 tcttcagtcc cattttcatt ttattccaaa gaattaactc caaataccaa tagaagccca
     9841 tcctctatat acttgagatt ccttactaac aatttcttgt aattctatta aagatttagg
     9901 taatccaaat ttatgtttta aaatatatct tccaagaaaa ctacttaatt tcatactaac
     9961 ttcaccaaca ctttttccct cactgatttc tttagaataa agaaagagct tttcttttaa
    10021 gtcaggatag tcatctatag ttgcttcatt gtatacttta ctaattgcat ttaaaagttt
    10081 ttcttcttta gtaaaatcca ttataataac ctccctacca ctaccttcta ggtgcccatg
    10141 gaccatgatt aacccaacca ttaacaataa tttttccaat ttctccttta gcttgattcc
    10201 aatctaccca acatttttct ttgttacaat aaagaccatt tccatagtaa gtagctgcat
    10261 caacttttat tcctgtaccg atgccagcta gacaagttcc taatattcct agaataacac
    10321 aatgttttaa tactttcttt ttcatgattg tttttccttt tcgttttttt aaaattgctt
    10381 gttatattaa ataatcttag taattttatt atataacatt ttattatata acaatttatt
    10441 atataacaat gattaacgtc ataaaaagta gttttgaatt cataatcgtt taaataaaat
    10501 tataagatca gttatttaaa cagctagtag acataatgtg tatactacca agtaacgcct
    10561 gtaccaaggg atccaggcgc tcactttgtg attttcgatt ggtctaagtc aaaagctctt
    10621 tttaaggctt ggttttctgc ggttgtttct ttctaattct ttgtgaatat tgatcggtca
    10681 ttttttaaat acttgttatt tttgtttgtg tttgttatgc tttttacata tctcatattc
    10741 atttattatt cttttctaag gttaataata ggataatctc tttcagaaag actgctcgta
    10801 cgagcagtct tttcttattt atcaagtttt tctacaaata cttcctcatt ttgactgttg
    10861 tagtaattag gattgagttt tagttcccct tttttatttc ctttgttggc cactgataat
    10921 aacattgggc taaagcttaa tattcctagt aggatagaac cgaatagcaa gtgttttttt
    10981 gtcataagta tttccttctt catttccata gctgccacca ttttttccaa ttctttgttt
    11041 gttcgtccat tgtttctgtt gcttcagaaa gagtcgttga actattctct gaagactctt
    11101 ccgtataagt caactgcagt tgctcctgaa gtttttcgat ttgttggttg gcttgcaggg
    11161 tgagttgttg ttgctggtcg agtagttttt gtatatgaga attattttgc tctttgaggg
    11221 caagttgttc tcgtaaaaat tcattttctt cacgcaactg atctagcaac ttgtttgcaa
    11281 ccacgttttt gtttggtata taagcatttt tatcgttgtt ttgttcatgt ttatgcaact
    11341 tatctagcaa cctttgattg tctaagttgc gagcaagttg caatactcca gaacgcaata
    11401 caaccttata accacgctca tttttagcta tcatggaaga atctaatttt tggatatgtt
    11461 tcgatattgc ttgcctactc atcttaaaaa gatctgacaa ttcctttatt gtaaaggttt
    11521 cttgtggttg catactggtt gcctcctagg ttgctactgg aataaattat ctaaatcaga
    11581 taataaactt tttttctctt tttccgaaag tgcggactct gtttgttttt caagagcttt
    11641 tttctctttt tctagttgtt tgatttgttg gaggagttca agttctcgtt tcttctgttt
    11701 gattttttcg ttaattgttg tagcgggttc ttcgtattct ttttgtttat cagctaaagc
    11761 caatgcttta ttttggtctt ctttggttgg tgcatgtttg ttaaagtcgt ctgctttttt
    11821 gatctcaggt ttgaacgtaa attgatagcc aataacaggt tttccccgtc cttttccgta
    11881 tttctttttg attgttagtc ctttgaagat tgcagttagt tcttgacgta taggttttat
    11941 aactcgttta tctatatcag gttgcttata acttttagga atatctaata attcaagaaa
    12001 gtcttctttg gagaaatatg cgaaaccttt ggttctgtat tgctttaaca gacgaaacat
    12061 agtctttgca tagccacttt gtagctcgtt gaattgttgt aaagaatagc gtacccactc
    12121 gtctaaatta tttaacaaag gcagtgcttt ttcgtaaatt cgtatatcta cgtaaggatt
    12181 ttctacgtcc ccgttgattt taaattctgt aaacattaca aacatatcgt aagaaagccc
    12241 agactcactt ctacgaccaa agcgtaaaga taagattttt tcataagttg cttttaaatc
    12301 ttctacaaat cgtacatttg cagttgcttt gtagttactt aaatctttta gttggtcaaa
    12361 cgtaaaacga actgtctt
//
