LOCUS       NZ_CP152083             2311 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Klebsiella pneumoniae strain PR_K2044 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP152083
VERSION     NZ_CP152083.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN41028361
            Assembly: GCF_038674545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Shi,L., Li,Z., Xiang,L., Tang,S., Lai,H., Xiong,X. and Zhou,Y.
  TITLE     The single amino acid substitution in WcaJ(S332L) promotes
            Hypervirulent Klebsiella pneumoniae resistant to bacteriophage and
            Virulence Attenuated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Shi,L., Li,Z., Xiang,L., Tang,S., Lai,H., Xiong,X. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2024) College of Basic Medical Sciences, Southwest
            Medical University, Zhongshan Road, Luzhou 646099, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v.2.
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038674545.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 23:51:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 4,932
            Genes (coding)                    :: 4,832
            CDSs (with protein)               :: 4,832
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 53 of 100
            Pseudo Genes (incomplete)         :: 44 of 100
            Pseudo Genes (internal stop)      :: 22 of 100
            Pseudo Genes (multiple problems)  :: 16 of 100
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152083.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="PR_K2044"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="China: Luzhou"
                     /lat_lon="28.89 N 105.44 E"
                     /collection_date="2023-03"
                     /collected_by="Pathogenic microorganism platform of
                     Southwest Medical University"
                     /note="after co-culturing phage vB_LZ2044 with the host
                     HvKP strain NTUH-K2044"
     gene            249..647
                     /locus_tag="AAGS55_RS25275"
                     /old_locus_tag="AAGS55_25275"
     CDS             249..647
                     /locus_tag="AAGS55_RS25275"
                     /old_locus_tag="AAGS55_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_258550859.1"
                     /translation="MKKDSFSGRWDEKALEMGWTAIPNALFFMQRPLNLSPTNFNVLLN
                     LFIHWWEAGTWPYPSQKGLASRMGVSVRTIQRALDEMTEMGFIKKTTTMRNHPRYKGRN
                     IYELTPVIHMIEIMAPELKKTIGKKKEI"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ctttgctgag catcattatg aagtactgtt aatttggaat aatgctgtat tgcttcggcc
       61 gtcccttgtg ggcggccttt tttttagctg caaaaagatc ataggttgta ggatcccgac
      121 aagcaatacg ccacaatgtc atactatatt aatattaata tagatatata ccggacagct
      181 gtgtttaaat gcgccttatc tcatagagat aaggcgcatt taaacacagc tgtccggtga
      241 tgtcacatat gaaaaaagat agtttcagtg gtcgttggga tgaaaaagca ctagagatgg
      301 gttggactgc catcccaaac gcgcttttct tcatgcagcg gcctttaaat ctttcgccaa
      361 caaattttaa cgtgctactt aatctcttca tacattggtg ggaagcaggt acatggcctt
      421 atccatctca gaaagggcta gcgtctcgca tgggggtctc tgtcagaacg atccaacgtg
      481 ctttggacga aatgactgag atgggattta taaaaaaaac aacgacgatg aggaatcacc
      541 ctagatacaa gggaagaaat atctatgaac taacccctgt catccacatg attgaaatca
      601 tggcaccaga gttaaaaaaa acgattggca aaaagaaaga gatttaacca cttggtgttt
      661 aaaacaagcg cgtatttacc ataacatccc ttgcccgcac cccctgagag cccctcagtg
      721 cgattctggc gcgggcgggc catttccggc acctttccct gtccatatcg ttaaaaatcg
      781 cgactgatga ttttggcgca cctgcatggc tatgcacgca ggtgaatggc aggcggttac
      841 agcataagcg cgcggtgaac gccccaaaag gggcactcta atcacgcaaa actgctttgc
      901 cagataaaga tcaccgcggc ggcgatgcta agcagtgagc cgatgatacc cagcaccacg
      961 ttaacatggc ttaaaaattc aaagtccatg taaacctcca ctaaaaagga cgatacgtga
     1021 tggccagttg actatcacga cagtatttag tgggaaaaaa ttaagcctct gacatgaacc
     1081 gctgagctga cagcacagct atggaaacgg tgaacgggcg cgaaattttt tcgcgttcgt
     1141 gaggcgttgg cggggtgtcg gggcgcagcc atgacgaagt gttttgggga tcgccaggct
     1201 aaggcggtac tccaaaaaca catcttgtcc cgttggtttt gtcgccggcg cgaagcggcg
     1261 gtaatgacaa aacatttcct gtccgcatca gttagtgcaa aaacgtaagt ggtgaaagat
     1321 tgcagtacag agcgacagga gcctgaggaa gcaccggaga aattacgctt cctcggtcat
     1381 tcgctcacta cgttcggtca tgactctgcg gcgagcgtta cgggctcaat cgaagggcag
     1441 tggaagtatg agatgtgaag tgtgaggagt tgctgcggaa atgccgtttt tccataggct
     1501 tcgccctcct gatgagcttc tcagaaatca ggcgatctcc ggtgctcaaa tcagaagagg
     1561 cgaaacccga caggacttaa agatccccac cgtttccgat gggtcgctcc ctcgtgcgct
     1621 ctcctgttcc caccctgagg tttacccgtt gcagaccgcc gtaaggtggc gttttctcat
     1681 tcctacccgt gcaactgagt tccttgtagg tggttcgctc caagctgggc tgtatgcacg
     1741 aactccccgt tcaacccgac tgccgcgtcc tcaaggtaac tgtaccctcg aatccaaccc
     1801 tttcagacac gataaatcgc cagtggcggc agccattggt aacagagatg tgcgagagat
     1861 attctgagaa ctcttgaagt agaagccaga ctgcggctac actggaggaa cagatttggt
     1921 gacatgtctc gcacaagact gttaccacgg ttcaaaaact tgccggttga tgaaccatcg
     1981 aaaaaccacc ttgccgggtg gttttttcgt tttcagggta cgcctgaaca gcaaatgccg
     2041 tcaagacacc tgccctccac catgaaaaat aaacaaaaat gtttatttcc gctcaaaaaa
     2101 tgagcagaaa atcatcatta ttgattgtaa ccattcgggt atacagaaaa gtgggatatg
     2161 attgctggga tgcctgaaaa gaatggcagt tgcgggagag gcccacctct tcgctgcaac
     2221 gaagaagcat ggcctctctg tgaatactca accgcagacc aggaggcgaa aatgctagaa
     2281 gatactcttc aagcgctcat cgcgctgctc a
//
