LOCUS       NZ_CP152077             3179 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Pseudomonas aeruginosa strain NCTR 501 plasmid pPsRE-2, complete
            sequence.
ACCESSION   NZ_CP152077
VERSION     NZ_CP152077.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19514382
            Assembly: GCF_019084125.3
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Kim,S.G., Wang,S.X., Sims,L. and Summage-West,C.V.
  TITLE     Complete genome sequence of Pseudomonas aeruginosa isolated from the
            inner ear of a mouse diagnosed with Otitis Media
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Kim,S.G., Wang,S.X., Sims,L. and Summage-West,C.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2024) Micro Surveillance, NCTR/FDA, 3900 NCTR Dr.,
            Jefferson, AR 72079, USA
REFERENCE   3  (bases 1 to 3179)
  AUTHORS   Kim,S.G., Wang,S.X., Sims,L. and Summage-West,C.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2024) Micro Surveillance, NCTR/FDA, 3900 NCTR Dr.,
            Jefferson, AR 72079, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2024
            Assembly Method                   :: Unicycler v. 03-2024
            Assembly Name                     :: PAeru_501_Uni_041824
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 353.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019084125.3-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 05:04:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,966
            CDSs (total)                      :: 5,885
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 30 of 49
            Pseudo Genes (internal stop)      :: 7 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152077.1.
            Bacteria and source DNA available from Sung G. Kim, NCTR/FDA, 3900
            NCTR Rd., Jefferson, AR 72079.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NCTR 501"
                     /isolation_source="inner ear"
                     /host="mouse"
                     /db_xref="taxon:287"
                     /plasmid="pPsRE-2"
                     /geo_loc_name="USA"
                     /collection_date="2016-06-15"
     gene            757..1866
                     /gene="wzz(fepE)"
                     /locus_tag="KNP07_RS29820"
                     /old_locus_tag="KNP07_29825"
     CDS             757..1866
                     /gene="wzz(fepE)"
                     /locus_tag="KNP07_RS29820"
                     /old_locus_tag="KNP07_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_005065596.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFALF
                     IKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            complement(2111..2245)
                     /locus_tag="KNP07_RS29825"
                     /old_locus_tag="KNP07_29830"
     CDS             complement(2111..2245)
                     /locus_tag="KNP07_RS29825"
                     /old_locus_tag="KNP07_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071606675.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY"
                     /name="replication initiation protein"
     gene            complement(2891..3055)
                     /locus_tag="KNP07_RS29830"
                     /old_locus_tag="KNP07_29835"
     CDS             complement(2891..3055)
                     /locus_tag="KNP07_RS29830"
                     /old_locus_tag="KNP07_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000090200.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          169..426
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          2725..2811
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(2174..2700)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
ORIGIN
        1 catcctgtcc tgtttcttta ggacgtatca tggcacagca tatcaactga taaataatcc
       61 ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc gtcgagtgtt tcttaatcaa
      121 taattccgct gatactccgg aaaaacgcga gtctgttctc atgcctgaaa tgcccacacc
      181 ccacgcaaaa acaagttttt gctgattttt ctttataaat agagagttat gacaaattag
      241 ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc ggcgcggtgt tgtagctgcg
      301 ccaacaccgc tttttagggg tggtactgac tattttcata aaaaaaacat tatcttatat
      361 taggggtgct gctagcggcg cggtgtgttt ttttacagga cgcccctggg ggcgctgcta
      421 ggggtgtctg ttcagatatg tagcaatgag tcagggattc acgaactgaa aatgtccgaa
      481 aatgtgtctc atgggcactg tatgcgccca aaccgatcgt cattcacttt catgcatagc
      541 tatgcagtga gctgaaagcg atcctgacgc atttttccgg tttaccccgg ggaaaacatc
      601 tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt tttagcggtg cgcgcaataa
      661 aatgttatgt taaatgtctg ctgaatggtt tttaggtttt ttcctaacat atgatagaat
      721 aaaaaaacca tatgttaata tattgtgttt ttttcaatga aaatctcaaa ggtctctgac
      781 gaacattttt ctaattacaa catgttgccg aatggtaatg aagaaattga cctttttggc
      841 ttgattagcg ttttatgggc agcaaaaata aaaattgttg gggtgattct attttttgct
      901 tgtattggct ttcttgtttc ttttgttttg ccacaaaaat ggactagttc tgctgtggtt
      961 actcctgctg agtctgttca atggagtgaa ttaaataagg agctttccaa gcttcatgtt
     1021 ctgggagtgg attttgatat caatcgtgat agcgcatttg ctctgtttat taagaaattt
     1081 cagtccgtta attcgttgaa tgagtatatg agatcatctc cttatgttat ggagattatt
     1141 aaaaagaata acattagcgc tctcgaactg catcgtgcaa ttgttgagtt gagtaaaaat
     1201 atgaagtcga ttgatgataa tgccagtaag aaaaacgaca agtcatcatt gtatgtatca
     1261 tggactctga gttttactgc tccaacaagt aaagaagctc acgatgtgtt gtctgggtat
     1321 attaattatg tttcttccct tgttgtaagg gatttgatgg aagatataag aaataaacta
     1381 gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg atgagataaa aattagaaac
     1441 cagctgaatg cagatattcg acgcctcaat tattcactgg aggttgctaa tgcggctgga
     1501 ataaaaaaac ctgtatacag caatggtcag attatgaagg atgacccaga ttttcctgtg
     1561 gctctcggtt ctgatggtat agcaactaaa ttgaacatca aaaaatcaat caaggatgtt
     1621 tcggaattga gtggggagtt gcgaaatcgt caatatgttg tgaatcaatt ggttgtggcg
     1681 aaagtggggg atgttgattt tatgccattt cagtatcaat tgagcccaac gttaccggtc
     1741 agaaaggatg ggccaagtaa tgccataatt gtaattcttt cttctgtcat tggagggatg
     1801 cttgcatgtg gctatatact tttacgtcat gccatggagg ccagaaagat gagtgtgatt
     1861 gagtaaaagt tgtgctgatg catcatttta taagcaactt ttataaaaag ttcaaaggat
     1921 cttcttgaga tcattccctt acagccgtag tcttctactc tagaaaacga aaaaaccacc
     1981 tggggaggtg gtttttcgaa ggttcagtaa gttggggaac ttctgaaccg tggtaacagt
     2041 cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta gcctcagtaa ggctgccact
     2101 tcaagaactc tcaatacatc tctcgcacat cctgtttacc agtggccgct gccaatggcg
     2161 ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2221 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaactgcc tacccggaac
     2281 tgagtgtcag gcgtggaatg agacgaacgc ggccataaca gcggaatgac accggtaaac
     2341 cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt
     2401 taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2461 gggggcggag cctatggaaa aacgccaccg gcgcggcctt ttttcggggc ctcttttttg
     2521 ctcacatgtt ttttctggtg ttatcccctg attctgtgga taaccgtata tccgtctttg
     2581 cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2641 aagcggaaga gcgccggaag ctgcattttc tccttacgta tctgtgcagc atttcacacc
     2701 tcatatgtcg ctgttctctc accatctgct ctgccgcctc ataattaagc cagtatacac
     2761 tccgttatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa aaccccctga
     2821 cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg ggaaagccgt gaccgcctcc
     2881 gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa gcgaatctgg atgctgttct
     2941 ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc cgcctgttcg tcggcatcca
     3001 gagcatccag tttttcaagc agtgtcaggc tctggctttt gatacatcgc gccatattca
     3061 gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc gtcagttccg ggtgtcgggg
     3121 gggggaccct gagcaggtgg taattgtaag acgttgcggg cgacggtatt acaattgca
//
