LOCUS       NZ_CP152076             4042 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Pseudomonas aeruginosa strain NCTR 501 plasmid pPsRE-1, complete
            sequence.
ACCESSION   NZ_CP152076
VERSION     NZ_CP152076.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19514382
            Assembly: GCF_019084125.3
KEYWORDS    RefSeq.
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Kim,S.G., Wang,S.X., Sims,L. and Summage-West,C.V.
  TITLE     Complete genome sequence of Pseudomonas aeruginosa isolated from the
            inner ear of a mouse diagnosed with Otitis Media
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Kim,S.G., Wang,S.X., Sims,L. and Summage-West,C.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2024) Micro Surveillance, NCTR/FDA, 3900 NCTR Dr.,
            Jefferson, AR 72079, USA
REFERENCE   3  (bases 1 to 4042)
  AUTHORS   Kim,S.G., Wang,S.X., Sims,L. and Summage-West,C.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2024) Micro Surveillance, NCTR/FDA, 3900 NCTR Dr.,
            Jefferson, AR 72079, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2024
            Assembly Method                   :: Unicycler v. 03-2024
            Assembly Name                     :: PAeru_501_Uni_041824
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 353.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019084125.3-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 05:04:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,966
            CDSs (total)                      :: 5,885
            Genes (coding)                    :: 5,836
            CDSs (with protein)               :: 5,836
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 30 of 49
            Pseudo Genes (internal stop)      :: 7 of 49
            Pseudo Genes (multiple problems)  :: 8 of 49
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP152076.1.
            Bacteria and source DNA available from Sung G. Kim, NCTR/FDA, 3900
            NCTR Rd., Jefferson, AR 72079.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="NCTR 501"
                     /isolation_source="inner ear"
                     /host="mouse"
                     /db_xref="taxon:287"
                     /plasmid="pPsRE-1"
                     /geo_loc_name="USA"
                     /collection_date="2016-06-15"
     gene            complement(<107..637)
                     /locus_tag="KNP07_RS29790"
                     /old_locus_tag="KNP07_29795"
                     /pseudo=""
     CDS             complement(<107..637)
                     /locus_tag="KNP07_RS29790"
                     /old_locus_tag="KNP07_29795"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(579..902)
                     /locus_tag="KNP07_RS29795"
                     /old_locus_tag="KNP07_29800"
     CDS             complement(579..902)
                     /locus_tag="KNP07_RS29795"
                     /old_locus_tag="KNP07_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956004.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            967..1158
                     /locus_tag="KNP07_RS29800"
                     /old_locus_tag="KNP07_29805"
     CDS             967..1158
                     /locus_tag="KNP07_RS29800"
                     /old_locus_tag="KNP07_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165994.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1760..1918
                     /locus_tag="KNP07_RS29805"
                     /old_locus_tag="KNP07_29810"
     CDS             1760..1918
                     /locus_tag="KNP07_RS29805"
                     /old_locus_tag="KNP07_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005093880.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            complement(3173..3406)
                     /locus_tag="KNP07_RS29810"
                     /old_locus_tag="KNP07_29815"
     CDS             complement(3173..3406)
                     /locus_tag="KNP07_RS29810"
                     /old_locus_tag="KNP07_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137443.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3413..3943)
                     /locus_tag="KNP07_RS29815"
                     /old_locus_tag="KNP07_29820"
     CDS             complement(3413..3943)
                     /locus_tag="KNP07_RS29815"
                     /old_locus_tag="KNP07_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067502.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1178..1264)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          1275..1832
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.853"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..589)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.959"
                     /coverage="99.0"
                     /evalue="6.16e-126"
     Typing          complement(3070..4040)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.914"
                     /coverage="99.0"
                     /evalue="1.1200000000000002e-179"
ORIGIN
        1 gcgcctgaat cacatcctga cggcctttta tctccccgac atgataaaga gcgtctatcc
       61 accgtgtaat gctctcagca agcgcctgtt tcgtttccgg caggttatca gggagagtca
      121 gcgtccggcg gttctccggg gcgttcgggt catgcagccc gtaatggtga ttcaccagcg
      181 tctgccaggc atcaattctc ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc
      241 cggtctgtag ctccatgttc ggaatgacaa aattcagctc aagccgcccc ttgtcctggt
      301 gctccaccca cagaatgctg tactgatttt tttcgagacc gggcatcagt acacgctcaa
      361 agctcgccat cactttttca cgccctcccg gcggcagctc cttctccgcg aacgagagaa
      421 caccggacgt gtatttcttc gcaaatggcg tggcatcgat gagttcccgg acttcttccg
      481 gattaccctg aagcaccgtt gccccttccc ggttacgctc ccttcccagc aggtaatcaa
      541 ccggaccact gccaccgcct tttcccctgg catgaaactt aactatcatc ccgcgtcccc
      601 tgttccctga cggccagacg cagccggcgc agctcatccc cgatggccat cagtgcggcc
      661 accacctgaa cccggtcacc ggaagaccac tgcccgctgt tcaccttcct cgctgcctga
      721 ttcaggttat tcccgatggc ggccagctga cgcagtaacg gcggtgccag tgtcggcagc
      781 ctcccggaac gggcaaccgg ctcacccagg cagacccgcc gcatccatac cgccagctgt
      841 ttaccctcac agcgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
      901 atcctctcgc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag
      961 gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     1021 acattaacgc tgctggagaa gctcaacgaa ctggacgcgg atgaacaggc agacatctgt
     1081 gaatcgcttt acgaccacgc cgatgagctt taccgcagtt gcctcgctcg cttcggggat
     1141 gacggtgaaa acctctgacg ggcttgtcag cgggtgttag cgggtgtcgg ggcgcagcca
     1201 tgacccagtc acgtagcgat agcggagtgt atactggctt aaccatgcgg catcagtgcg
     1261 gattgtatga aaagtgcgcc ataccgggtg tgaaatgccg cacagatgcg taaggagaaa
     1321 atgcacgtcc aggcgctttt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     1381 actgcggcga gcggtatcta ctcactcaaa aacgaaatgc tgttatccac agaatcaggg
     1441 gataacgccg gaaagaacat gtgagcaaaa gaccaggaac aggaagaagg ccacgccgat
     1501 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaaccgac gctcaagtca
     1561 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1621 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1681 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt
     1741 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1801 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttaacgccac tggcagcagc
     1861 cattggtaac tggacaaggt ggattgagat attaagagtt cttgaagtgg tggcctaact
     1921 gcggctacac tagaaggaca gcatttggta tctgtgctcc accaagccag ttaccttcgg
     1981 aagaaagagt tggtagctct agattccggc aaacaaacca ccgttggtag cggtggtttt
     2041 tttgtttgca ggcagcagat tacgcgccgc aaaaaaggat ctcaagaaga tcttgaatca
     2101 ctctatgaaa attttgtttt atagccgtat ttgttgatgc tataagccag agaccttcta
     2161 tgagaataga catatcacat caaacacggc ataccccgcc aaacatgctt cctcgggaac
     2221 aaaattgtgt agcaatggca ttatctgcgt gctttagaca acaactaaac cctgtggtaa
     2281 acagcttatt aaaagagaga ataatacact cccctaaaga attggaacat gacaacgcag
     2341 taataagagt cctgcaaaaa ctacaaattc aagaagtttg taacagcacc ctttgggaga
     2401 cagccaaaca acaactactt caaaaatcgg acggaagata ttttgccatc aattctaaac
     2461 acttagcctt tccaggccca ggagaatctc acgccttctg ttgcatcaaa tacaaaaatg
     2521 caattggcat taacggaaac aatgccgaaa cccaatcgac ccactaccaa ccctatccct
     2581 atgataaagt cagtatatgg ggaccattcc ctcacaattt aacgtaatat cttaattgta
     2641 cgcaccgctg aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa
     2701 ccacccgaaa aactgccgcg atcgcacccg gtaaatttta accacatgca tagctatgca
     2761 gccatgtgaa tcacgcagga attgccagcc agagacagct gaaacggatt ttattcatgc
     2821 tttcggaagc gaagaagacg gggacctgag cgggaaaaca gaagttcagg ggaatgagcg
     2881 cgatctggca atagaggcaa aacacagcga caaaagacac accagaatcg cgcccgtatg
     2941 cgttttaact cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc
     3001 cgagccaacc attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc
     3061 tctgattacc atcccagcga agggccatcc agtgtccgtt cctgtatttc cggctggcgt
     3121 tcccgttcca ggaataacgc atgttcgcgc tcctgtatca accgttcctc tcttatctcc
     3181 agttctcgct gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc agcacctgct
     3241 gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca
     3301 ttttctgcaa ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt
     3361 gcagctgctc taatgcgttc agcaaatggc tttccagctc cgtcatgctc tgtcactcct
     3421 gtaccagcct tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca
     3481 tcccctcggg caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg
     3541 tcttcgcgtt cagcttctcc agactctcgt tctgcttcct gatttcttcc aggttcgcct
     3601 gtatcatccc gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca
     3661 tccccagtaa catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca
     3721 tcatcgcctc tttcacccct tcccgatgtt ttttcagtga ttcctctgtc gaagctgtga
     3781 acagggctat agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt
     3841 cttcgagctt cctgaactcg tcgctcagca ttttctctgt agattcggct ctctctttca
     3901 gctttttctc gaactccgcg cccgtctgta aaagattgct catagaatgc tcctttcagt
     3961 ctgatattct tcccgccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc
     4021 acttcaagcc ccgcctctgt ga
//
