LOCUS       NZ_CP151394             8401 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R15.0423 plasmid pR15.0423_8k, complete
            sequence.
ACCESSION   NZ_CP151394
VERSION     NZ_CP151394.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39735790
            Assembly: GCF_038367755.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038367755.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:40:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,363
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 720
            CDSs (without protein)            :: 720
            Pseudo Genes (ambiguous residues) :: 0 of 720
            Pseudo Genes (frameshifted)       :: 202 of 720
            Pseudo Genes (incomplete)         :: 517 of 720
            Pseudo Genes (internal stop)      :: 124 of 720
            Pseudo Genes (multiple problems)  :: 116 of 720
            Pseudo Genes (short protein)      :: 3 of 720
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151394.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R15.0423"
                     /db_xref="taxon:624"
                     /plasmid="pR15.0423_8k"
                     /collected_by="Taiwan CDC"
     gene            complement(1..159)
                     /locus_tag="AADX55_RS26610"
                     /old_locus_tag="AADX55_26635"
     CDS             complement(1..159)
                     /locus_tag="AADX55_RS26610"
                     /old_locus_tag="AADX55_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            730..879
                     /locus_tag="AADX55_RS26615"
                     /old_locus_tag="AADX55_26640"
     CDS             730..879
                     /locus_tag="AADX55_RS26615"
                     /old_locus_tag="AADX55_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(1646..2086)
                     /locus_tag="AADX55_RS26620"
                     /old_locus_tag="AADX55_26645"
     CDS             complement(1646..2086)
                     /locus_tag="AADX55_RS26620"
                     /old_locus_tag="AADX55_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            2304..2693
                     /locus_tag="AADX55_RS26625"
                     /old_locus_tag="AADX55_26650"
     CDS             2304..2693
                     /locus_tag="AADX55_RS26625"
                     /old_locus_tag="AADX55_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2737..>2925
                     /locus_tag="AADX55_RS26630"
                     /old_locus_tag="AADX55_26655"
                     /pseudo=""
     CDS             2737..>2925
                     /locus_tag="AADX55_RS26630"
                     /old_locus_tag="AADX55_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2892..4091)
                     /gene="tet(A)"
                     /locus_tag="AADX55_RS26635"
                     /old_locus_tag="AADX55_26660"
     ARG             complement(2896..4091)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4197..4847
                     /gene="tetR(A)"
                     /locus_tag="AADX55_RS26640"
                     /old_locus_tag="AADX55_26665"
     CDS             4197..4847
                     /gene="tetR(A)"
                     /locus_tag="AADX55_RS26640"
                     /old_locus_tag="AADX55_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(4879..5121)
                     /locus_tag="AADX55_RS26645"
                     /old_locus_tag="AADX55_26670"
     CDS             complement(4879..5121)
                     /locus_tag="AADX55_RS26645"
                     /old_locus_tag="AADX55_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5427..6263)
                     /locus_tag="AADX55_RS26650"
                     /old_locus_tag="AADX55_26675"
     CDS             complement(5427..6263)
                     /locus_tag="AADX55_RS26650"
                     /old_locus_tag="AADX55_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(6263..7066)
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX55_RS26655"
                     /old_locus_tag="AADX55_26680"
     ARG             complement(6267..7066)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7127..7942)
                     /gene="sul2"
                     /locus_tag="AADX55_RS26660"
                     /old_locus_tag="AADX55_26685"
     ARG             complement(7131..7942)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5431..6263)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          522..695
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(51..598)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.848"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tcaggcctct acttcaagac ctcttaatct atcttctcgt gcactcctgt tactaatggc
       61 tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
      121 caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
      181 tgccttcacg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggaat
      241 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa
      301 cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttt
      361 gcgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatctct
      421 tctttgctaa gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc cgtaagccgg
      481 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
      541 tctcctgtat cgcatattct gctgacgcgc cggtgctgcc ttttttctcc tgccacatga
      601 agcacttcac tgattttcgc aatcgtggca acatagtaag ccagtataca ctccgctagc
      661 gctacatgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc agctactgga
      721 tatcacccca tgccatctga atatgatgaa caactggatt cgactgatac gcagcttccc
      781 ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct gcatgcccct
      841 gtggattttg ggtcgcctgt cggccgggaa gcgttatagc tttttcgctt gcccgcgtac
      901 gtggtaatac tccggtatta ccttttattt ctttattttc atgtaattac agtggatttt
      961 taatacccgg gtattactcg tgaaaacgaa atattccttt tttttctttt aattcatgca
     1021 gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg aaaaataatc
     1081 aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa gcgttcctca
     1141 ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat tacatcgctc
     1201 cttcagttca tacatcactt ttacatcact tatacatcac ttatacatca cttatacatc
     1261 acttttacat cattccttca gctcatacgt cactttcatc ctctgtgcca tttgtacatc
     1321 acccccttcc tgaacgtgct gacagcgggt tttccggctg cgccggcaac gcttcacggc
     1381 cacgcaataa attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga
     1441 cttaccccgg ctgaatggtg tgaatttccg cgctatgcac ttgtgcgcat actcatgcat
     1501 gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt
     1561 accggtttcc cgtcagaaac gccctgaggg cctctcaggc ggtgctgata acagatgtta
     1621 tggtaaatca gagtcacctg cactgttact ctgaaccgga gcgaaacagc cgcgaccaga
     1681 atccccgctt tttttcctgt gccagcgcgt cagccagacg gctaatttcg gcctgtagtt
     1741 gttccctctc ctcatttcgc aggcggtttt ctgcttcccg cgccatcttg tcttcaagca
     1801 ttagggtaac agtctgctgc agagcgtcca gtttctgctg aatgacgtct agctgagcat
     1861 tttccgcctg gctaacgaca gatttggagt gtccctcctt gtgccttttt ggtgtctcag
     1921 aagtgcgcag ctcgccatag gcccgaatca gttcactggt ctcgaactcc cggcgatcat
     1981 tttttcctgt gcgatagctg acaaggcctt tagccatgtc acgatataat tgcgagcgcg
     2041 actttcctgt taatttcagt gtctcttgaa ctgtgtgcca tgccataaaa gtgacagtgt
     2101 ctcctgtgtg taccgtattg taacagcaga acatattact gttttaacac aaaaggtctg
     2161 aggctgcccg tgaggcgtga aaaatacaca aaaaacctgt tgacggtgtg gttttgacgg
     2221 gcgtagtatt tcgaatactg tatataaaca cattgttata aatacagttt ttgctgtttt
     2281 taaaagtcaa agaggataat tttatgagta cagagttaaa tagcggtact gtcggtggtt
     2341 ttggtcacat gggaccaaat gacccctaca atgatggtca tagcggctct ggaagttcgg
     2401 gaggtagttt taacggtggt ggtaatggtg gccatgataa tagtagtggt tcatcttata
     2461 ctgcatcctt tggtctcatc actggccgac acccgataac tgtcccaggt acgaagaata
     2521 ttaattctgt cgatctttct gctgtcatta ataacattat acatcatgaa ggtgcacgca
     2581 ataacatgta cctagatact gcgaacaatg taagagtggg agtgacgggc actggctggc
     2641 aatgtctagc aacggcaggc atttcggctg agggtaaaag aactttccgc taagcgatag
     2701 actgtatgta aacacagtat tgcaaggacg cggaacatgc ctcatgtggc ggccaggacg
     2761 gccagccggg atcgggatac tggtcgttac cagagccacc gacccgagca aacccttctc
     2821 tatcagatcg ttgacgagta ttacccggca ttcgctgcgc ttatggcaga gcagggaaag
     2881 gaattgccgg gctatgtgca acgggaattt gaagaatttc tccaaagccc gcgacgcagc
     2941 gccggcaggc agagcaagta gagggcagcg cctgcaatcc atgcccaccc gttccacgtt
     3001 gttatagaag ccgcatagat cgccgtgaag aggaggggtc cgacgatcga ggtcaggctg
     3061 gtgagcgccg ccagtgagcc ttgcagctgc ccctgacgtt cctcatccac ctgcctggac
     3121 aacattgctt gcagcgccgg cattccgatg ccacccgaag caagcaggac catgatcggg
     3181 aacgccatcc atccccgtgt cgcgaaggca agcaggatgt agcctgtgcc gtcggcaatc
     3241 attccgagca tgagtgcccg cctttcgccg agccgggcgg ctacagggcc ggtgatcatt
     3301 gcctgggcga gtgaatgcag aatgccaaat gcggcaagcg aaatgccgat cgtggtcgcg
     3361 tcccagtgaa agcgatcctc gccgaaaatg acccaaagcg cggccggcac ctgtccgaca
     3421 agttgcatga tgaagaagac cgccatcagg gcggcgacga cggtcatgcc ccgggcccac
     3481 cggaacgaag cgagcgggtt gagagcctcc cggcgtaacg gccggcgttc gcctttgtgc
     3541 gactccggca aaaggaaaca gcccgtcagg aaattgaggc cgttcaaggc tgccgcggcg
     3601 aagaacggag cgtgggggga gaaaccgccc atcagcccac cgagcacagg tcccgcgacc
     3661 atcccgaacc cgaaacaggc gctcatgaag ccgaagtgcc gcgcgcgctc atcgccatca
     3721 gtgatatcgg caatataagc gccggctacc gccccagtcg ccccggtgat gccggccacg
     3781 atccgcccga tatagagaac ccaaaggaaa ggcgccgtcg ccatgatggc gtagtcgaca
     3841 gcagcgccgg ccagcgagac gagcaagacc ggccgccgcc cgaaacgatc cgacagcgcg
     3901 cccagcacag gtgcgcaggc aaattgcatc aacgcataca gcgccagcag aatgccatag
     3961 tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag caccggcata
     4021 atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat caggggtctg
     4081 ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac gcgcggattc
     4141 tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg tcaagcatga
     4201 caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg
     4261 tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag ccggcgcttt
     4321 actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg
     4381 agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt ctgatcggga
     4441 atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg
     4501 gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg
     4561 aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc tacttcactg
     4621 ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc ggcggcaccg
     4681 ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc gacgaagccg
     4741 gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg gcgaaaagga
     4801 ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca ggaccgctgc
     4861 cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc cccctttcca
     4921 ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc ctagcgtcca
     4981 agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcctgct gcggcgtccg
     5041 ctcgtgggcc gtggcgcggg tccgcgcgcc ggcctcgtgc gcctggcgct cgcgggcgag
     5101 gtccagggcg gccgtcttca cgttctgcct tgcgcagatg agatagatct agcgtggact
     5161 caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg ggcaccgcag
     5221 ggaaattctc gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt
     5281 ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca ggggtcgttt gcgggaaggg
     5341 gcggaatcct acgctaaggc tttggccagc gatattctcc ggtgagattg atgtgttccc
     5401 aggggatagg agaagtcgct tgatatctag tatgacgtct gtcgcacctg cttgatcgcg
     5461 gccgcgatag ctagatcgcg ttgctcctct tctccatccg cgttccaagc tgcggaaagg
     5521 cacccataag cgtacgcctg gtcgagcagg cgacgcggat cgacgtccag cgcacgagag
     5581 aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac aaaggtcgtc tctgtcagcc
     5641 ggatcgtaga acatattggc ggcgccaaag cccacttcac cgaccagacc gacgggatct
     5701 atcaccagcc agccgcgact ggagaacatg atgttttcat gatgcagatc gccatgtagc
     5761 ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta taatcgccgc gtggacgtag
     5821 tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct gaaacaaagc tgcaaagcga
     5881 tcccggatcg ggagaagggc agaaggcagg ggttcctcag atgcggcata cagcttcgcc
     5941 attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag
     6001 agcattcgct ccccggcata ttcgagcaac atcagattgt tctcacgacc gagcaaccgg
     6061 actgctcccc tcccattgcg ccataccaga tagtcggccc cgcgcagttc atcagcaatg
     6121 tcttctatag gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc
     6181 caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca
     6241 ggaaaaacag gcggcatgaa catcaacccc aagtcagagg gtccaatcgc agatagaagg
     6301 caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc
     6361 gctctgcttc atctggcgct gcccagttct cttcggcgtt agcaatcatg agtgccaaat
     6421 cggcatagcg atctgctgtt ccgagccgcc caaggtcgat cagacccgtg cattgaagag
     6481 ttttagggtc caccatgaag ttcggcatgc agggatcacc atggcaaaca accatatcgg
     6541 tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa agatcgagct
     6601 gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg cgggacacca
     6661 catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaacggacat tgatcaaccg
     6721 ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg agcaaatccg
     6781 ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag catgcaccct
     6841 cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct ttgagccaaa
     6901 tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg attttcgcga
     6961 aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca
     7021 accagtcaga atgcgattca ccaaaaaaaa tattagttcg attcaatgga ggttccttca
     7081 gttttctgat gaagcgcgaa tatagagaaa tatcccgaat gtgcagttaa cgaattcttg
     7141 cggtttcttt cagcgccgcc aataccgcca gcccgtcgcg caaggggcgc ggctcgtgtg
     7201 tgcggatgaa gtcagctcca cctgcggcgg cggcaagctc tgcagcgagt gtcgcggccc
     7261 cgacatcccc cggaccacgg cctgtgagcg cgcgcagaaa ggatttgcgc gaaacagaca
     7321 gaagcaccgg caaatcgaag cgcagccgca attcatcgaa ccgcgccagc accgagagcg
     7381 aggtttcggg agcagccccc agaaaaaacc ccatgccggg atcaaggaca aggcggttgc
     7441 gtttgatacc ggcacccgtc agcgccgcga tgcgcgcgtc aaagaacgcc gcaatgtgat
     7501 ccatgatgtc gccagcgggt gcctcgcgcc gatctgcctg cccgtcttgc accgaatgca
     7561 taacgacgag tttggcagat gatttcgcca attgcggata gaacgcagcg tctggaaaac
     7621 cgcgaatatc attgagatag gccacaccac gcgacaaggc ataggcttgc gtcgcgggtt
     7681 gataactgtc gagcgagacg ggaatgccat ctgccttgag cgcgtccagc accggcgcga
     7741 tacgcgcgat ttctgtgtcg gacgaaacag gcgcggcgtc gggattgctg gatgccggac
     7801 cgaggtcgat cacatctgcc ccctcggcca tcagcttacg cgcctgcgca atggctgcgt
     7861 ctggcgccag ataccggcct ccatcggaga aactgtccga ggttatgttg acgatgccga
     7921 aaatgatgag cgatttattc atgggggctt ctataataat aataatcgag catgagtctc
     7981 atacggatgc tcgggtcgaa agggaatccc caggcgagta acctgtttgc ggtgatccgt
     8041 ttcatgatga tgtatctgta gcattttgga aataaatata tcatctctca ataagtagat
     8101 atatttatct tttttgcttc tttggtgctg caagtcacca atacgaggga aacctgttac
     8161 ggagcggttt aaccgtaatc aacagtgact ggtgtcatgt tcggaagatt aattgatagt
     8221 aatctacaaa ggatcttctt gagatccttt tcaatgaatc gtaatctctt gctctgtaaa
     8281 cgaaaaaacc gcctggggag gcggtttgtt cgaaggttaa gtcagttggg gaactgctta
     8341 acccggtaac agggttcacg agaccgccat taccaaaact gttctttcag tgtagccgca
     8401 g
//
