LOCUS       NZ_CP151386             8401 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R15.0763 plasmid pR15.0763_8k, complete
            sequence.
ACCESSION   NZ_CP151386
VERSION     NZ_CP151386.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39735791
            Assembly: GCF_038368245.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038368245.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:34:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,050
            CDSs (total)                      :: 4,922
            Genes (coding)                    :: 4,282
            CDSs (with protein)               :: 4,282
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 640
            CDSs (without protein)            :: 640
            Pseudo Genes (ambiguous residues) :: 0 of 640
            Pseudo Genes (frameshifted)       :: 168 of 640
            Pseudo Genes (incomplete)         :: 445 of 640
            Pseudo Genes (internal stop)      :: 113 of 640
            Pseudo Genes (multiple problems)  :: 84 of 640
            Pseudo Genes (short protein)      :: 3 of 640
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151386.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R15.0763"
                     /db_xref="taxon:624"
                     /plasmid="pR15.0763_8k"
                     /collected_by="Taiwan CDC"
     gene            1..441
                     /locus_tag="AADX53_RS25115"
                     /old_locus_tag="AADX53_25100"
     CDS             1..441
                     /locus_tag="AADX53_RS25115"
                     /old_locus_tag="AADX53_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1208..1357)
                     /locus_tag="AADX53_RS25120"
                     /old_locus_tag="AADX53_25105"
     CDS             complement(1208..1357)
                     /locus_tag="AADX53_RS25120"
                     /old_locus_tag="AADX53_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1928..2086
                     /locus_tag="AADX53_RS25125"
                     /old_locus_tag="AADX53_25110"
     CDS             1928..2086
                     /locus_tag="AADX53_RS25125"
                     /old_locus_tag="AADX53_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2546..3361
                     /gene="sul2"
                     /locus_tag="AADX53_RS25130"
                     /old_locus_tag="AADX53_25115"
     ARG             2547..3358
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3422..4225
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX53_RS25135"
                     /old_locus_tag="AADX53_25120"
     ARG             3423..4222
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            4225..5061
                     /locus_tag="AADX53_RS25140"
                     /old_locus_tag="AADX53_25125"
     CDS             4225..5061
                     /locus_tag="AADX53_RS25140"
                     /old_locus_tag="AADX53_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5367..5609
                     /locus_tag="AADX53_RS25145"
                     /old_locus_tag="AADX53_25130"
     CDS             5367..5609
                     /locus_tag="AADX53_RS25145"
                     /old_locus_tag="AADX53_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5641..6291)
                     /gene="tetR(A)"
                     /locus_tag="AADX53_RS25150"
                     /old_locus_tag="AADX53_25135"
     CDS             complement(5641..6291)
                     /gene="tetR(A)"
                     /locus_tag="AADX53_RS25150"
                     /old_locus_tag="AADX53_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            6397..7596
                     /gene="tet(A)"
                     /locus_tag="AADX53_RS25155"
                     /old_locus_tag="AADX53_25140"
     ARG             6398..7593
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7563..7751)
                     /locus_tag="AADX53_RS25160"
                     /old_locus_tag="AADX53_25145"
                     /pseudo=""
     CDS             complement(<7563..7751)
                     /locus_tag="AADX53_RS25160"
                     /old_locus_tag="AADX53_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7795..8184)
                     /locus_tag="AADX53_RS25165"
                     /old_locus_tag="AADX53_25150"
     CDS             complement(7795..8184)
                     /locus_tag="AADX53_RS25165"
                     /old_locus_tag="AADX53_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             4226..5058
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1393..1566)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1490..2095
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta
       61 tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag
      121 ttcgagacca gtgaactgat tcgggcctat ggcgagctgc gcacttctga gacaccaaaa
      181 aggcacaagg agggacactc caaatctgtc gttagccagg cggaaaatgc tcagctagac
      241 gtcattcagc agaaactgga cgctctgcag cagactgtta ccctaatgct tgaagacaag
      301 atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga gggaacaact acaggccgaa
      361 attagccgtc tggctgacgc gctggcacag gaaaaaaagc ggggattctg gtcgcggctg
      421 tttcgctccg gttcagagta acagtgcagg tgactctgat ttaccataac atctgttatc
      481 agcaccgcct gagaggccct cagggcgttt ctgacgggaa accggtaaaa caggcaacaa
      541 accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc
      601 gcacaagtgc atagcgcgga aattcacacc attcagccgg ggtaagtcct gtttccggcg
      661 ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg
      721 ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg tacaaatggc
      781 acagaggatg aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg tataagtgat
      841 gtaaaagtga tgtataagtg atgtaaaagt gatgtatgaa ctgaaggagc gatgtaattc
      901 tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag gaacgctttt
      961 tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt atttttcaac
     1021 ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca tgaattaaaa
     1081 gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa tccactgtaa
     1141 ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac gcgggcaagc
     1201 gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg gcatgcaggt
     1261 tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga agctgcgtat
     1321 cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca gtagctgaga
     1381 ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta gcggagtgta
     1441 tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat gtggcaggag
     1501 aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt ccgcttcctc
     1561 gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg cttacggacg
     1621 gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag ataaggccac
     1681 ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa tctgacgctc
     1741 aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag
     1801 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     1861 gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc gctccaagct
     1921 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg gtaactatca
     1981 acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca ttagtaacag
     2041 gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc ggctacactg
     2101 aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa gcagttcccc
     2161 aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt acagagcaag
     2221 agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact atcaattaat
     2281 cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta acaggtttcc
     2341 ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc tacttattga
     2401 gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg gatcaccgca
     2461 aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc
     2521 gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa
     2581 cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg cagccattgc
     2641 gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc cggcatccag
     2701 caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct
     2761 ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac ccgcgacgca
     2821 agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt ttccagacgc
     2881 tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc attcggtgca
     2941 agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc acattgcggc
     3001 gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca accgccttgt
     3061 ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc tctcggtgct
     3121 ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg
     3181 caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg ccgcgacact
     3241 cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac acgagccgcg
     3301 ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta
     3361 actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga aggaacctcc
     3421 attgaatcga actaatattt tttttggtga atcgcattct gactggttgc ctgtcagagg
     3481 cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg cgaaaatcgc
     3541 acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt ggctcaaagg
     3601 tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt
     3661 ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg atttgctcaa
     3721 agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg ttgatcaatg
     3781 tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa
     3841 tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt
     3901 ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg atatggttgt
     3961 ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc ttcaatgcac
     4021 gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt tggcactcat
     4081 gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg ccttcgctgt
     4141 cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg
     4201 attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc tcattggcac
     4261 gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa agtttcattg
     4321 ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa
     4381 ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg gttgctcggt
     4441 cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc tcacatcgtt
     4501 gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc gaagctgtat
     4561 gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg ctttgcagct
     4621 ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg
     4681 gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct acatggcgat
     4741 ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga tcccgtcggt
     4801 ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc tgacagagac
     4861 gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc tcgtgcgctg
     4921 gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct ttccgcagct
     4981 tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc gatcaagcag
     5041 gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg aacacatcaa
     5101 tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa
     5161 cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag ggcgcaggct
     5221 cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg cggtgcccct
     5281 caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt ccacgctaga
     5341 tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc gcccgcgagc
     5401 gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc
     5461 aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     5521 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     5581 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     5641 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     5701 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     5761 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     5821 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     5881 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     5941 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     6001 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     6061 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     6121 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     6181 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     6241 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     6301 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     6361 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc
     6421 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     6481 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     6541 ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     6601 cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc
     6661 atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc
     6721 accggggcga ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag
     6781 cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct
     6841 gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc
     6901 ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa
     6961 cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc
     7021 atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg
     7081 ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc
     7141 ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc
     7201 cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc
     7261 acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc
     7321 ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg
     7381 gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg
     7441 atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg
     7501 tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg
     7561 ctttggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt tccctgctct
     7621 gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag aagggtttgc
     7681 tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt cctggccgcc
     7741 acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg
     7801 gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc
     7861 cgtcactccc actcttacat tgttcgcagt atctaggtac atgttattgc gtgcaccttc
     7921 atgatgtata atgttattaa tgacagcaga aagatcgaca gaattaatat tcttcgtacc
     7981 tgggacagtt atcgggtgtc ggccagtgat gagaccaaag gatgcagtat aagatgaacc
     8041 actactatta tcatggccac cattaccacc accgttaaaa ctacctcccg aacttccaga
     8101 gccgctatga ccatcattgt aggggtcatt tggtcccatg tgaccaaaac caccgacagt
     8161 accgctattt aactctgtac tcataaaatt atcctctttg acttttaaaa acagcaaaaa
     8221 ctgtatttat aacaatgtgt ttatatacag tattcgaaat actacgcccg tcaaaaccac
     8281 accgtcaaca ggttttttgt gtatttttca cgcctcacgg gcagcctcag accttttgtg
     8341 ttaaaacagt aatatgttct gctgttacaa tacggtacac acaggagaca ctgtcacttt
     8401 t
//
