LOCUS       NZ_CP151375             8401 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R15.1832 plasmid pR15.1832_8k, complete
            sequence.
ACCESSION   NZ_CP151375
VERSION     NZ_CP151375.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40122663
            Assembly: GCF_038367355.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038367355.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:29:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,063
            Genes (coding)                    :: 4,437
            CDSs (with protein)               :: 4,437
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 626
            CDSs (without protein)            :: 626
            Pseudo Genes (ambiguous residues) :: 0 of 626
            Pseudo Genes (frameshifted)       :: 168 of 626
            Pseudo Genes (incomplete)         :: 435 of 626
            Pseudo Genes (internal stop)      :: 108 of 626
            Pseudo Genes (multiple problems)  :: 82 of 626
            Pseudo Genes (short protein)      :: 3 of 626
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151375.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R15.1832"
                     /db_xref="taxon:624"
                     /plasmid="pR15.1832_8k"
                     /collected_by="Taiwan CDC"
     gene            459..1274
                     /gene="sul2"
                     /locus_tag="AADX60_RS25780"
                     /old_locus_tag="AADX60_25780"
     ARG             460..1271
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1335..2138
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX60_RS25785"
                     /old_locus_tag="AADX60_25785"
     ARG             1336..2135
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2138..2974
                     /locus_tag="AADX60_RS25790"
                     /old_locus_tag="AADX60_25790"
     CDS             2138..2974
                     /locus_tag="AADX60_RS25790"
                     /old_locus_tag="AADX60_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            3280..3522
                     /locus_tag="AADX60_RS25795"
                     /old_locus_tag="AADX60_25795"
     CDS             3280..3522
                     /locus_tag="AADX60_RS25795"
                     /old_locus_tag="AADX60_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(3554..4204)
                     /gene="tetR(A)"
                     /locus_tag="AADX60_RS25800"
                     /old_locus_tag="AADX60_25800"
     CDS             complement(3554..4204)
                     /gene="tetR(A)"
                     /locus_tag="AADX60_RS25800"
                     /old_locus_tag="AADX60_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            4310..5509
                     /gene="tet(A)"
                     /locus_tag="AADX60_RS25805"
                     /old_locus_tag="AADX60_25805"
     ARG             4311..5506
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<5476..5664)
                     /locus_tag="AADX60_RS25810"
                     /old_locus_tag="AADX60_25810"
                     /pseudo=""
     CDS             complement(<5476..5664)
                     /locus_tag="AADX60_RS25810"
                     /old_locus_tag="AADX60_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5708..6097)
                     /locus_tag="AADX60_RS25815"
                     /old_locus_tag="AADX60_25815"
     CDS             complement(5708..6097)
                     /locus_tag="AADX60_RS25815"
                     /old_locus_tag="AADX60_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            6315..6755
                     /locus_tag="AADX60_RS25820"
                     /old_locus_tag="AADX60_25820"
     CDS             6315..6755
                     /locus_tag="AADX60_RS25820"
                     /old_locus_tag="AADX60_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(7522..7671)
                     /locus_tag="AADX60_RS25825"
                     /old_locus_tag="AADX60_25825"
     CDS             complement(7522..7671)
                     /locus_tag="AADX60_RS25825"
                     /old_locus_tag="AADX60_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             2139..2971
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7707..7880)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          7804..8351
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.848"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tgcggctaca ctgaaagaac agttttggta atggcggtct cgtgaaccct gttaccgggt
       61 taagcagttc cccaactgac ttaaccttcg aacaaaccgc ctccccaggc ggttttttcg
      121 tttacagagc aagagattac gattcattga aaaggatctc aagaagatcc tttgtagatt
      181 actatcaatt aatcttccga acatgacacc agtcactgtt gattacggtt aaaccgctcc
      241 gtaacaggtt tccctcgtat tggtgacttg cagcaccaaa gaagcaaaaa agataaatat
      301 atctacttat tgagagatga tatatttatt tccaaaatgc tacagataca tcatcatgaa
      361 acggatcacc gcaaacaggt tactcgcctg gggattccct ttcgacccga gcatccgtat
      421 gagactcatg ctcgattatt attattatag aagcccccat gaataaatcg ctcatcattt
      481 tcggcatcgt caacataacc tcggacagtt tctccgatgg aggccggtat ctggcgccag
      541 acgcagccat tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg atcgacctcg
      601 gtccggcatc cagcaatccc gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta
      661 tcgcgccggt gctggacgcg ctcaaggcag atggcattcc cgtctcgctc gacagttatc
      721 aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg
      781 gttttccaga cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta
      841 tgcattcggt gcaagacggg caggcagatc ggcgcgaggc acccgctggc gacatcatgg
      901 atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac
      961 gcaaccgcct tgtccttgat cccggcatgg ggttttttct gggggctgct cccgaaacct
     1021 cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc
     1081 tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg
     1141 gggccgcgac actcgctgca gagcttgccg ccgccgcagg tggagctgac ttcatccgca
     1201 cacacgagcc gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg
     1261 caagaattcg ttaactgcac attcgggata tttctctata ttcgcgcttc atcagaaaac
     1321 tgaaggaacc tccattgaat cgaactaata ttttttttgg tgaatcgcat tctgactggt
     1381 tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg acgtggtgac gggcatgcct
     1441 tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca
     1501 tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat caactggcag gaggaacagg
     1561 agggtgcatg cttggtgata acggcaattc cgggagtacc ggcggctgat ctgtctggag
     1621 cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat
     1681 cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg
     1741 tggtgtcccg caatgccgtc aatcccgact tcttaccgga cgaggacaag agtacgccgc
     1801 agctcgatct tttggctcgt gtcgaacgag agctaccggt gcggctcgac caagagcgca
     1861 ccgatatggt tgtttgccat ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa
     1921 ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg aacagcagat cgctatgccg
     1981 atttggcact catgattgct aacgccgaag agaactgggc agcgccagat gaagcagagc
     2041 gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg
     2101 ccttctatct gcgattggac cctctgactt ggggttgatg ttcatgccgc ctgtttttcc
     2161 tgctcattgg cacgtttcgc aacctgttct cattgcggac accttttcca gcctcgtttg
     2221 gaaagtttca ttgccagacg ggactcctgc aatcgtcaag ggattgaaac ctatagaaga
     2281 cattgctgat gaactgcgcg gggccgacta tctggtatgg cgcaatggga ggggagcagt
     2341 ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg agcgaatgct
     2401 ctctcacatc gttgccgagc acggcgacta ccaggcgacc gaaattgcag cggaactaat
     2461 ggcgaagctg tatgccgcat ctgaggaacc cctgccttct gcccttctcc cgatccggga
     2521 tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga
     2581 ctacgtccac gcggcgatta tagccgatca aatgatgagc aatgcctcgg aactgcgtgg
     2641 gctacatggc gatctgcatc atgaaaacat catgttctcc agtcgcggct ggctggtgat
     2701 agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc
     2761 ggctgacaga gacgaccttt gtctcgatcc tagacgcatt gcacagatgg cggacgcatt
     2821 ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg cttatgggtg
     2881 cctttccgca gcttggaacg cggatggaga agaggagcaa cgcgatctag ctatcgcggc
     2941 cgcgatcaag caggtgcgac agacgtcata ctagatatca agcgacttct cctatcccct
     3001 gggaacacat caatctcacc ggagaatatc gctggccaaa gccttagcgt aggattccgc
     3061 cccttcccgc aaacgacccc tggaagcccc gtagcgacgc ggagaggggc gagacaagcc
     3121 aagggcgcag gctcgatgcg cagcacgaca tagccggttc tcgcaaggac gagaatttcc
     3181 ctgcggtgcc cctcaagtgt caatgaaagt ttccaacgcg agccattcgc gagagccttg
     3241 agtccacgct agatctatct catctgcgca aggcagaacg tgaagacggc cgccctggac
     3301 ctcgcccgcg agcgccaggc gcacgaggcc ggcgcgcgga cccgcgccac ggcccacgag
     3361 cggacgccgc agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct
     3421 tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg
     3481 tggaaagggg gaggggatgt tgtctacatg gctctgctgt agtgagtggg ttgcgctccg
     3541 gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc aacgttcctg acaacgagcc
     3601 tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct gcgtccggac
     3661 cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa
     3721 cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc acggccccaa
     3781 cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa aaacccgcct
     3841 cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc
     3901 cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat
     3961 tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc gtatgattct
     4021 ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt
     4081 aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta
     4141 cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc tgcaactttg
     4201 tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat cagtgataaa
     4261 gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagacg tgaaacccaa
     4321 cagacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca tcggcctgat
     4381 tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg tcaccgccca
     4441 ctatggcatt ctgctggcgc tgtatgcgtt gatgcaattt gcctgcgcac ctgtgctggg
     4501 cgcgctgtcg gatcgtttcg ggcggcggcc ggtcttgctc gtctcgctgg ccggcgctgc
     4561 tgtcgactac gccatcatgg cgacggcgcc tttcctttgg gttctctata tcgggcggat
     4621 cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg ccgatatcac
     4681 tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat
     4741 ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg ctccgttctt
     4801 cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt tgccggagtc
     4861 gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg cttcgttccg
     4921 gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact
     4981 tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct ttcactggga
     5041 cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac tcgcccaggc
     5101 aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca tgctcggaat
     5161 gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat ggatggcgtt
     5221 cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt
     5281 gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac
     5341 cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg cttctataac
     5401 aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct gcctgccggc
     5461 gctgcgtcgc gggctttgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc
     5521 ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata
     5581 gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc
     5641 cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt
     5701 ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt
     5761 gccagccagt gcccgtcact cccactctta cattgttcgc agtatctagg tacatgttat
     5821 tgcgtgcacc ttcatgatgt ataatgttat taatgacagc agaaagatcg acagaattaa
     5881 tattcttcgt acctgggaca gttatcgggt gtcggccagt gatgagacca aaggatgcag
     5941 tataagatga accactacta ttatcatggc caccattacc accaccgtta aaactacctc
     6001 ccgaacttcc agagccgcta tgaccatcat tgtaggggtc atttggtccc atgtgaccaa
     6061 aaccaccgac agtaccgcta tttaactctg tactcataaa attatcctct ttgactttta
     6121 aaaacagcaa aaactgtatt tataacaatg tgtttatata cagtattcga aatactacgc
     6181 ccgtcaaaac cacaccgtca acaggttttt tgtgtatttt tcacgcctca cgggcagcct
     6241 cagacctttt gtgttaaaac agtaatatgt tctgctgtta caatacggta cacacaggag
     6301 acactgtcac ttttatggca tggcacacag ttcaagagac actgaaatta acaggaaagt
     6361 cgcgctcgca attatatcgt gacatggcta aaggccttgt cagctatcgc acaggaaaaa
     6421 atgatcgccg ggagttcgag accagtgaac tgattcgggc ctatggcgag ctgcgcactt
     6481 ctgagacacc aaaaaggcac aaggagggac actccaaatc tgtcgttagc caggcggaaa
     6541 atgctcagct agacgtcatt cagcagaaac tggacgctct gcagcagact gttaccctaa
     6601 tgcttgaaga caagatggcg cgggaagcag aaaaccgcct gcgaaatgag gagagggaac
     6661 aactacaggc cgaaattagc cgtctggctg acgcgctggc acaggaaaaa aagcggggat
     6721 tctggtcgcg gctgtttcgc tccggttcag agtaacagtg caggtgactc tgatttacca
     6781 taacatctgt tatcagcacc gcctgagagg ccctcagggc gtttctgacg ggaaaccggt
     6841 aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttacggc
     6901 atgcatgagt atgcgcacaa gtgcatagcg cggaaattca caccattcag ccggggtaag
     6961 tcctgtttcc ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg
     7021 gccgtgaagc gttgccggcg cagccggaaa acccgctgtc agcacgttca ggaagggggt
     7081 gatgtacaaa tggcacagag gatgaaagtg acgtatgagc tgaaggaatg atgtaaaagt
     7141 gatgtataag tgatgtataa gtgatgtata agtgatgtaa aagtgatgta tgaactgaag
     7201 gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg aacgatacag
     7261 tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc cgccatggtt
     7321 gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt tcatcgcaac
     7381 tgcatgaatt aaaagaaaaa aaaggaatat ttcgttttca cgagtaatac ccgggtatta
     7441 aaaatccact gtaattacat gaaaataaag aaataaaagg taataccgga gtattaccac
     7501 gtacgcgggc aagcgaaaaa gctataacgc ttcccggccg acaggcgacc caaaatccac
     7561 aggggcatgc aggttctctt cagtaacctg cgccattaaa tcaaaaaggt catattcgcc
     7621 gggaagctgc gtatcagtcg aatccagttg ttcatcatat tcagatggca tggggtgata
     7681 tccagtagct gagaggttgt tagcgggttt cggggcgcag ccctgaacca gtcatgtagc
     7741 gctagcggag tgtatactgg cttactatgt tgccacgatt gcgaaaatca gtgaagtgct
     7801 tcatgtggca ggagaaaaaa ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga
     7861 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta
     7921 ccggcttacg gacggggacg gaaatttcct ggaagatacc aggaagacac ttagcaaaga
     7981 agagataagg ccacggcgaa gccgtttttc cataggctcc gcccccctga caagcatcgc
     8041 aaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg
     8101 tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     8161 attccgctgt tatggccgcg gtttctcatt ccacgcctga cactcagttc cgtgaaggca
     8221 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg
     8281 tccggtaact atcaacttga gtccaacccg gaaagacacg acaaaacgcc actggcagca
     8341 gccattagta acaggagtgc acgagaagat agattaagag gtcttgaagt agaggcctga
     8401 c
//
