LOCUS       NZ_CP151366             8379 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R16.0937 plasmid pR16.0937_8k, complete
            sequence.
ACCESSION   NZ_CP151366
VERSION     NZ_CP151366.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40122664
            Assembly: GCF_038367185.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038367185.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:38:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,030
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,266
            CDSs (with protein)               :: 4,266
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 635
            CDSs (without protein)            :: 635
            Pseudo Genes (ambiguous residues) :: 0 of 635
            Pseudo Genes (frameshifted)       :: 172 of 635
            Pseudo Genes (incomplete)         :: 435 of 635
            Pseudo Genes (internal stop)      :: 111 of 635
            Pseudo Genes (multiple problems)  :: 81 of 635
            Pseudo Genes (short protein)      :: 3 of 635
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151366.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R16.0937"
                     /db_xref="taxon:624"
                     /plasmid="pR16.0937_8k"
                     /collected_by="Taiwan CDC"
     gene            1..441
                     /locus_tag="AADX59_RS24980"
                     /old_locus_tag="AADX59_24990"
     CDS             1..441
                     /locus_tag="AADX59_RS24980"
                     /old_locus_tag="AADX59_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(1186..1335)
                     /locus_tag="AADX59_RS24985"
                     /old_locus_tag="AADX59_24995"
     CDS             complement(1186..1335)
                     /locus_tag="AADX59_RS24985"
                     /old_locus_tag="AADX59_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1906..2064
                     /locus_tag="AADX59_RS24990"
                     /old_locus_tag="AADX59_25000"
     CDS             1906..2064
                     /locus_tag="AADX59_RS24990"
                     /old_locus_tag="AADX59_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2524..3339
                     /gene="sul2"
                     /locus_tag="AADX59_RS24995"
                     /old_locus_tag="AADX59_25005"
     ARG             2525..3336
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3400..4203
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX59_RS25000"
                     /old_locus_tag="AADX59_25010"
     ARG             3401..4200
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            4203..5039
                     /locus_tag="AADX59_RS25005"
                     /old_locus_tag="AADX59_25015"
     CDS             4203..5039
                     /locus_tag="AADX59_RS25005"
                     /old_locus_tag="AADX59_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5345..5587
                     /locus_tag="AADX59_RS25010"
                     /old_locus_tag="AADX59_25020"
     CDS             5345..5587
                     /locus_tag="AADX59_RS25010"
                     /old_locus_tag="AADX59_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5619..6269)
                     /gene="tetR(A)"
                     /locus_tag="AADX59_RS25015"
                     /old_locus_tag="AADX59_25025"
     CDS             complement(5619..6269)
                     /gene="tetR(A)"
                     /locus_tag="AADX59_RS25015"
                     /old_locus_tag="AADX59_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            6375..7574
                     /gene="tet(A)"
                     /locus_tag="AADX59_RS25020"
                     /old_locus_tag="AADX59_25030"
     ARG             6376..7571
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7541..7729)
                     /locus_tag="AADX59_RS25025"
                     /old_locus_tag="AADX59_25035"
                     /pseudo=""
     CDS             complement(<7541..7729)
                     /locus_tag="AADX59_RS25025"
                     /old_locus_tag="AADX59_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7773..8162)
                     /locus_tag="AADX59_RS25030"
                     /old_locus_tag="AADX59_25040"
     CDS             complement(7773..8162)
                     /locus_tag="AADX59_RS25030"
                     /old_locus_tag="AADX59_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             4204..5036
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1371..1544)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1468..2073
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta
       61 tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag
      121 ttcgagacca gtgaactgat tcgggcctat ggcgagctgc gcacttctga gacaccaaaa
      181 aggcacaagg agggacactc caaatctgtc gttagccagg cggaaaatgc tcagctagac
      241 gtcattcagc agaaactgga cgctctgcag cagactgtta ccctaatgct tgaagacaag
      301 atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga gggaacaact acaggccgaa
      361 attagccgtc tggctgacgc gctggcacag gaaaaaaagc ggggattctg gtcgcggctg
      421 tttcgctccg gttcagagta acagtgcagg tgactctgat ttaccataac atctgttatc
      481 agcaccgcct gagaggccct cagggcgttt ctgacgggaa accggtaaaa caggcaacaa
      541 accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc
      601 gcacaagtgc atagcgcgga aattcacacc attcagccgg ggtaagtcct gtttccggcg
      661 ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg
      721 ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg tacaaatggc
      781 acagaggatg aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg tataagtgat
      841 gtaaaagtga tgtatgaact gaaggagcga tgtaattctg tagtcaggaa gaataacccg
      901 ttcatgcagg catgaacgat acagtgagga acgctttttt tgtcacgatc tccccttaac
      961 ctgcgtaaat cacccgccat ggttgattat ttttcaacct caaaatggtc atacacctaa
     1021 cgcagccgcg attttcatcg caactgcatg aattaaaaga aaaaaaagga atatttcgtt
     1081 ttcacgagta atacccgggt attaaaaatc cactgtaatt acatgaaaat aaagaaataa
     1141 aaggtaatac cggagtatta ccacgtacgc gggcaagcga aaaagctata acgcttcccg
     1201 gccgacaggc gacccaaaat ccacaggggc atgcaggttc tcttcagtaa cctgcgccat
     1261 taaatcaaaa aggtcatatt cgccgggaag ctgcgtatca gtcgaatcca gttgttcatc
     1321 atattcagat ggcatggggt gatatccagt agctgagagg ttgttagcgg gtttcggggc
     1381 gcagccctga accagtcatg tagcgctagc ggagtgtata ctggcttact atgttgccac
     1441 gattgcgaaa atcagtgaag tgcttcatgt ggcaggagaa aaaaggcagc accggcgcgt
     1501 cagcagaata tgcgatacag gagatattcc gcttcctcgc tcactgactc gctacgctcg
     1561 gtcgttcggc tgcggcgagc ggtaccggct tacggacggg gacggaaatt tcctggaaga
     1621 taccaggaag acacttagca aagaagagat aaggccacgg cgaagccgtt tttccatagg
     1681 ctccgccccc ctgacaagca tcgcaaaatc tgacgctcaa atcagtggtg gcgaaacccg
     1741 acaggactat aaagatccca ggcgtttccc cctggtagct ccctcgtgcg ctctcctgtt
     1801 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcggtttct cattccacgc
     1861 ctgacactca gttccgtgaa ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1921 gttcagtccg accgctgcgc cttgtccggt aactatcaac ttgagtccaa cccggaaaga
     1981 cacgacaaaa cgccactggc agcagccatt agtaacagga gtgcacgaga agatagatta
     2041 agaggtcttg aagtagaggc ctgactgcgg ctacactgaa agaacagttt tggtaatggc
     2101 ggtctcgtga accctgttac cgggttaagc agttccccaa ctgacttaac cttcgaacaa
     2161 accgcctccc caggcggttt tttcgtttac agagcaagag attacgattc attgaaaagg
     2221 atctcaagaa gatcctttgt agattactat caattaatct tccgaacatg acaccagtca
     2281 ctgttgatta cggttaaacc gctccgtaac aggtttccct cgtattggtg acttgcagca
     2341 ccaaagaagc aaaaaagata aatatatcta cttattgaga gatgatatat ttatttccaa
     2401 aatgctacag atacatcatc atgaaacgga tcaccgcaaa caggttactc gcctggggat
     2461 tccctttcga cccgagcatc cgtatgagac tcatgctcga ttattattat tatagaagcc
     2521 cccatgaata aatcgctcat cattttcggc atcgtcaaca taacctcgga cagtttctcc
     2581 gatggaggcc ggtatctggc gccagacgca gccattgcgc aggcgcgtaa gctgatggcc
     2641 gagggggcag atgtgatcga cctcggtccg gcatccagca atcccgacgc cgcgcctgtt
     2701 tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc
     2761 attcccgtct cgctcgacag ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt
     2821 gtggcctatc tcaatgatat tcgcggtttt ccagacgctg cgttctatcc gcaattggcg
     2881 aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag acgggcaggc agatcggcgc
     2941 gaggcacccg ctggcgacat catggatcac attgcggcgt tctttgacgc gcgcatcgcg
     3001 gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc ttgatcccgg catggggttt
     3061 tttctggggg ctgctcccga aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg
     3121 ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc
     3181 acaggccgtg gtccggggga tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc
     3241 gcaggtggag ctgacttcat ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg
     3301 gtattggcgg cgctgaaaga aaccgcaaga attcgttaac tgcacattcg ggatatttct
     3361 ctatattcgc gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt
     3421 tttggtgaat cgcattctga ctggttgcct gtcagaggcg gagaatctgg tgattttgtt
     3481 tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag
     3541 ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag
     3601 gtcatcaact ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga
     3661 gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag
     3721 caacttggcg ctgttcacag cctatcggtt gatcaatgtc agtttgagcg caggctgtcg
     3781 cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta
     3841 ccggacgagg acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta
     3901 ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg
     3961 ccgaacttca tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg
     4021 ctcggaacag cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac
     4081 tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc
     4141 gaagcccccg accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt
     4201 tgatgttcat gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg
     4261 cggacacctt ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg
     4321 tcaagggatt gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg
     4381 tatggcgcaa tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc
     4441 tcgaatatgc cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg
     4501 cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc cgcatctgag gaacccctgc
     4561 cttctgccct tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg
     4621 atcaaaacgc aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga
     4681 tgagcaatgc ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt
     4741 tctccagtcg cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg
     4801 gcgccgccaa tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac
     4861 gcattgcaca gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc
     4921 tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg
     4981 agcaacgcga tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga
     5041 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
     5101 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg acccctggaa gccccgtagc
     5161 gacgcggaga ggggcgagac aagccaaggg cgcaggctcg atgcgcagca cgacatagcc
     5221 ggttctcgca aggacgagaa tttccctgcg gtgcccctca agtgtcaatg aaagtttcca
     5281 acgcgagcca ttcgcgagag ccttgagtcc acgctagatc tatctcatct gcgcaaggca
     5341 gaacgtgaag acggccgccc tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc
     5401 gcggacccgc gccacggccc acgagcggac gccgcagcag gagcgccaga aggccgccag
     5461 agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa agcccgtagc
     5521 gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct acatggctct
     5581 gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt
     5641 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
     5701 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
     5761 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
     5821 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
     5881 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
     5941 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
     6001 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
     6061 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
     6121 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
     6181 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
     6241 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     6301 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     6361 tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc actgtcgcgc
     6421 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     6481 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttgatgc
     6541 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct
     6601 tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg gcgcctttcc
     6661 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     6721 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     6781 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     6841 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     6901 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     6961 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     7021 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     7081 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     7141 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     7201 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     7261 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     7321 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     7381 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     7441 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     7501 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagaaat tcttcaaatt
     7561 cccgttgcac atagcccggc aattcctttc cctgctctgc cataagcgca gcgaatgccg
     7621 ggtaatactc gtcaacgatc tgatagagaa gggtttgctc gggtcggtgg ctctggtaac
     7681 gaccagtatc ccgatcccgg ctggccgtcc tggccgccac atgaggcatg ttccgcgtcc
     7741 ttgcaatact gtgtttacat acagtctatc gcttagcgga aagttctttt accctcagcc
     7801 gaaatgcctg ccgttgctag acattgccag ccagtgcccg tcactcccac tcttacattg
     7861 ttcgcagtat ctaggtacat gttattgcgt gcaccttcat gatgtataat gttattaatg
     7921 acagcagaaa gatcgacaga attaatattc ttcgtacctg ggacagttat cgggtgtcgg
     7981 ccagtgatga gaccaaagga tgcagtataa gatgaaccac tactattatc atggccacca
     8041 ttaccaccac cgttaaaact acctcccgaa cttccagagc cgctatgacc atcattgtag
     8101 gggtcatttg gtcccatgtg accaaaacca ccgacagtac cgctatttaa ctctgtactc
     8161 ataaaattat cctctttgac ttttaaaaac agcaaaaact gtatttataa caatgtgttt
     8221 atatacagta ttcgaaatac tacgcccgtc aaaaccacac cgtcaacagg ttttttgtgt
     8281 atttttcacg cctcacgggc agcctcagac cttttgtgtt aaaacagtaa tatgttctgc
     8341 tgttacaata cggtacacac aggagacact gtcactttt
//
