LOCUS       NZ_CP151351             8412 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R17.0261 plasmid pR17.0261_8k, complete
            sequence.
ACCESSION   NZ_CP151351
VERSION     NZ_CP151351.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40122666
            Assembly: GCF_038367155.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038367155.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:46:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,103
            CDSs (total)                      :: 4,973
            Genes (coding)                    :: 4,344
            CDSs (with protein)               :: 4,344
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 629
            CDSs (without protein)            :: 629
            Pseudo Genes (ambiguous residues) :: 0 of 629
            Pseudo Genes (frameshifted)       :: 169 of 629
            Pseudo Genes (incomplete)         :: 437 of 629
            Pseudo Genes (internal stop)      :: 110 of 629
            Pseudo Genes (multiple problems)  :: 84 of 629
            Pseudo Genes (short protein)      :: 3 of 629
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151351.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R17.0261"
                     /db_xref="taxon:624"
                     /plasmid="pR17.0261_8k"
                     /collected_by="Taiwan CDC"
     gene            1..150
                     /locus_tag="AADX58_RS25360"
                     /old_locus_tag="AADX58_25380"
     CDS             1..150
                     /locus_tag="AADX58_RS25360"
                     /old_locus_tag="AADX58_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(928..1368)
                     /locus_tag="AADX58_RS25365"
                     /old_locus_tag="AADX58_25385"
     CDS             complement(928..1368)
                     /locus_tag="AADX58_RS25365"
                     /old_locus_tag="AADX58_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            1586..1975
                     /locus_tag="AADX58_RS25370"
                     /old_locus_tag="AADX58_25390"
     CDS             1586..1975
                     /locus_tag="AADX58_RS25370"
                     /old_locus_tag="AADX58_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2019..>2207
                     /locus_tag="AADX58_RS25375"
                     /old_locus_tag="AADX58_25395"
                     /pseudo=""
     CDS             2019..>2207
                     /locus_tag="AADX58_RS25375"
                     /old_locus_tag="AADX58_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2174..3373)
                     /gene="tet(A)"
                     /locus_tag="AADX58_RS25380"
                     /old_locus_tag="AADX58_25400"
     ARG             complement(2178..3373)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3479..4129
                     /gene="tetR(A)"
                     /locus_tag="AADX58_RS25385"
                     /old_locus_tag="AADX58_25405"
     CDS             3479..4129
                     /gene="tetR(A)"
                     /locus_tag="AADX58_RS25385"
                     /old_locus_tag="AADX58_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(4161..4403)
                     /locus_tag="AADX58_RS25390"
                     /old_locus_tag="AADX58_25410"
     CDS             complement(4161..4403)
                     /locus_tag="AADX58_RS25390"
                     /old_locus_tag="AADX58_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4709..5545)
                     /locus_tag="AADX58_RS25395"
                     /old_locus_tag="AADX58_25415"
     CDS             complement(4709..5545)
                     /locus_tag="AADX58_RS25395"
                     /old_locus_tag="AADX58_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5545..6348)
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX58_RS25400"
                     /old_locus_tag="AADX58_25420"
     ARG             complement(5549..6348)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6409..7224)
                     /gene="sul2"
                     /locus_tag="AADX58_RS25405"
                     /old_locus_tag="AADX58_25425"
     ARG             complement(6413..7224)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7684..7842)
                     /locus_tag="AADX58_RS25410"
                     /old_locus_tag="AADX58_25430"
     CDS             complement(7684..7842)
                     /locus_tag="AADX58_RS25410"
                     /old_locus_tag="AADX58_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             complement(4713..5545)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          8205..8378
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(7676..8281)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgccatctg aatatgatga acaactggat tcgactgata cgcagcttcc cggcgaatat
       61 gacctttttg atttaatggc gcaggttact gaagagaacc tgcatgcccc tgtggatttt
      121 gggtcgcctg tcggccggga agcgttatag ctttttcgct tgcccgcgta cgtggtaata
      181 ctccggtatt accttttatt tctttatttt catgtaatta cagtggattt ttaatacccg
      241 ggtattactc gtgaaaacga aatattcctt ttttttcttt taattcatgc agttgcgatg
      301 aaaatcgcgg ctgcgttagg tgtatgacca ttttgaggtt gaaaaataat caaccatggc
      361 gggtgattta cgcaggttaa ggggagatcg tgacaaaaaa agcgttcctc actgtatcgt
      421 tcatgcctgc atgaacgggt tattcttcct gactacagaa ttacatcgct ccttcagttc
      481 atacatcact tttacatcac ttatacatca cttatacatc acttatacat cacttttaca
      541 tcacttttac atcattcctt cagctcatac gtcactttca tcctctgtgc catttgtaca
      601 tcaccccctt cctgaacgtg ctgacagcgg gttttccggc tgcgccggca acgcttcacg
      661 gccacgcaat aaattgcgtc cccgttcacc gttttcggat gggaagaacg ccggaaacag
      721 gacttacccc ggctgaatgg tgtgaatttc cgcgctatgc acttgtgcgc atactcatgc
      781 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
      841 ttaccggttt cccgtcagaa acgccctgag ggcctctcag gcggtgctga taacagatgt
      901 tatggtaaat cagagtcacc tgcactgtta ctctgaaccg gagcgaaaca gccgcgacca
      961 gaatccccgc tttttttcct gtgccagcgc gtcagccaga cggctaattt cggcctgtag
     1021 ttgttccctc tcctcatttc gcaggcggtt ttctgcttcc cgcgccatct tgtcttcaag
     1081 cattagggta acagtctgct gcagagcgtc cagtttctgc tgaatgacgt ctagctgagc
     1141 attttccgcc tggctaacga cagatttgga gtgtccctcc ttgtgccttt ttggtgtctc
     1201 agaagtgcgc agctcgccat aggcccgaat cagttcactg gtctcgaact cccggcgatc
     1261 attttttcct gtgcgatagc tgacaaggcc tttagccatg tcacgatata attgcgagcg
     1321 cgactttcct gttaatttca gtgtctcttg aactgtgtgc catgccataa aagtgacagt
     1381 gtctcctgtg tgtaccgtat tgtaacagca gaacatatta ctgttttaac acaaaaggtc
     1441 tgaggctgcc cgtgaggcgt gaaaaataca caaaaaacct gttgacggtg tggttttgac
     1501 gggcgtagta tttcgaatac tgtatataaa cacattgtta taaatacagt ttttgctgtt
     1561 tttaaaagtc aaagaggata attttatgag tacagagtta aatagcggta ctgtcggtgg
     1621 ttttggtcac atgggaccaa atgaccccta caatgatggt catagcggct ctggaagttc
     1681 gggaggtagt tttaacggtg gtggtaatgg tggccatgat aatagtagtg gttcatctta
     1741 tactgcatcc tttggtctca tcactggccg acacccgata actgtcccag gtacgaagaa
     1801 tattaattct gtcgatcttt ctgctgtcat taataacatt atacatcatg aaggtgcacg
     1861 caataacatg tacctagata ctgcgaacaa tgtaagagtg ggagtgacgg gcactggctg
     1921 gcaatgtcta gcaacggcag gcatttcggc tgagggtaaa agaactttcc gctaagcgat
     1981 agactgtatg taaacacagt attgcaagga cgcggaacat gcctcatgtg gcggccagga
     2041 cggccagccg ggatcgggat actggtcgtt accagagcca ccgacccgag caaacccttc
     2101 tctatcagat cgttgacgag tattacccgg cattcgctgc gcttatggca gagcagggaa
     2161 aggaattgcc gggctatgtg caacgggaat ttgaagaatt tctccaaagc ccgcgacgca
     2221 gcgccggcag gcagagcaag tagagggcag cgcctgcaat ccatgcccac ccgttccacg
     2281 ttgttataga agccgcatag atcgccgtga agaggagggg tccgacgatc gaggtcaggc
     2341 tggtgagcgc cgccagtgag ccttgcagct gcccctgacg ttcctcatcc acctgcctgg
     2401 acaacattgc ttgcagcgcc ggcattccga tgccacccga agcaagcagg accatgatcg
     2461 ggaacgccat ccatccccgt gtcgcgaagg caagcaggat gtagcctgtg ccgtcggcaa
     2521 tcattccgag catgagtgcc cgcctttcgc cgagccgggc ggctacaggg ccggtgatca
     2581 ttgcctgggc gagtgaatgc agaatgccaa atgcggcaag cgaaatgccg atcgtggtcg
     2641 cgtcccagtg aaagcgatcc tcgccgaaaa tgacccaaag cgcggccggc acctgtccga
     2701 caagttgcat gatgaagaag accgccatca gggcggcgac gacggtcatg ccccgggccc
     2761 accggaacga agcgagcggg ttgagagcct cccggcgtaa cggccggcgt tcgcctttgt
     2821 gcgactccgg caaaaggaaa cagcccgtca ggaaattgag gccgttcaag gctgccgcgg
     2881 cgaagaacgg agcgtggggg gagaaaccgc ccatcagccc accgagcaca ggtcccgcga
     2941 ccatcccgaa cccgaaacag gcgctcatga agccgaagtg ccgcgcgcgc tcatcgccat
     3001 cagtgatatc ggcaatataa gcgccggcta ccgccccagt cgccccggtg atgccggcca
     3061 cgatccgccc gatatagaga acccaaagga aaggcgccgt cgccatgatg gcgtagtcga
     3121 cagcagcgcc ggccagcgag acgagcaaga ccggccgccg cccgaaacga tccgacagcg
     3181 cgcccagcac aggtgcgcag gcaaattgca tcaacgcata cagcgccagc agaatgccat
     3241 agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc agcaccggca
     3301 taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg atcaggggtc
     3361 tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga acgcgcggat
     3421 tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag tgtcaagcat
     3481 gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga acgaggtcgg
     3541 cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc agccggcgct
     3601 ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag ccatgctggc
     3661 ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat ttctgatcgg
     3721 gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc
     3781 cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc gcttcctctg
     3841 cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca gctacttcac
     3901 tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc gcggcggcac
     3961 cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc
     4021 cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat tggcgaaaag
     4081 gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gacgattgat caggaccgct
     4141 gccggagcgc aacccactca ctacagcaga gccatgtaga caacatcccc tccccctttc
     4201 caccgcgtca gacgcccgta gcagcccgct acgggctttt tcatgccctg ccctagcgtc
     4261 caagcctcac ggccgcgctc ggcctctctg gcggccttct ggcgctcctg ctgcggcgtc
     4321 cgctcgtggg ccgtggcgcg ggtccgcgcg ccggcctcgt gcgcctggcg ctcgcgggcg
     4381 aggtccaggg cggccgtctt cacgttctgc cttgcgcaga tgagatagat ctagcgtgga
     4441 ctcaaggctc tcgcgaatgg ctcgcgttgg aaactttcat tgacacttga ggggcaccgc
     4501 agggaaattc tcgtccttgc gagaaccggc tatgtcgtgc tgcgcatcga gcctgcgccc
     4561 ttggcttgtc tcgcccctct ccgcgtcgct acggggcttc caggggtcgt ttgcgggaag
     4621 gggcggaatc ctacgctaag gctttggcca gcgatattct ccggtgagat tgatgtgttc
     4681 ccaggggata ggagaagtcg cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg
     4741 cggccgcgat agctagatcg cgttgctcct cttctccatc cgcgttccaa gctgcggaaa
     4801 ggcacccata agcgtacgcc tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag
     4861 agaatgcgtc cgccatctgt gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag
     4921 ccggatcgta gaacatattg gcggcgccaa agcccacttc accgaccaga ccgacgggat
     4981 ctatcaccag ccagccgcga ctggagaaca tgatgttttc atgatgcaga tcgccatgta
     5041 gcccacgcag ttccgaggca ttgctcatca tttgatcggc tataatcgcc gcgtggacgt
     5101 agtcagtttg acaacctgcg ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc
     5161 gatcccggat cgggagaagg gcagaaggca ggggttcctc agatgcggca tacagcttcg
     5221 ccattagttc cgctgcaatt tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag
     5281 agagcattcg ctccccggca tattcgagca acatcagatt gttctcacga ccgagcaacc
     5341 ggactgctcc cctcccattg cgccatacca gatagtcggc cccgcgcagt tcatcagcaa
     5401 tgtcttctat aggtttcaat cccttgacga ttgcaggagt cccgtctggc aatgaaactt
     5461 tccaaacgag gctggaaaag gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag
     5521 caggaaaaac aggcggcatg aacatcaacc ccaagtcaga gggtccaatc gcagatagaa
     5581 ggcaaggcgt tcgcggtcgg gggcttcgat ccccaataca ttgaatagga cagcgaaggc
     5641 gcgctctgct tcatctggcg ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa
     5701 atcggcatag cgatctgctg ttccgagccg cccaaggtcg atcagacccg tgcattgaag
     5761 agttttaggg tccaccatga agttcggcat gcagggatca ccatggcaaa caaccatatc
     5821 ggtgcgctct tggtcgagcc gcaccggtag ctctcgttcg acacgagcca aaagatcgag
     5881 ctgcggcgta ctcttgtcct cgtccggtaa gaagtcggga ttgacggcat tgcgggacac
     5941 cacatcaacg gcgcgtccga acattcgcga cagcctgcgc tcaaacggac attgatcaac
     6001 cgataggctg tgaacagcgc caagttgctg ccccattgac ggccacgctt tgagcaaatc
     6061 cgctccagac agatcagccg ccggtactcc cggaattgcc gttatcacca agcatgcacc
     6121 ctcctgttcc tcctgccagt tgatgacctc ggggcaagcc acacctcgac ctttgagcca
     6181 aatgaggcgg tcacgctctc cagcgagctc accgcggcgg gaagcaggtg cgattttcgc
     6241 gaaggcatgc ccgtcaccac gtcgaaaaac aaaatcacca gattctccgc ctctgacagg
     6301 caaccagtca gaatgcgatt caccaaaaaa aatattagtt cgattcaatg gaggttcctt
     6361 cagttttctg atgaagcgcg aatatagaga aatatcccga atgtgcagtt aacgaattct
     6421 tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc gcggctcgtg
     6481 tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc
     6541 cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga
     6601 cagaagcacc ggcaaatcga agcgcagccg caattcatcg aaccgcgcca gcaccgagag
     6661 cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt
     6721 gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg
     6781 atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg
     6841 cataacgacg agtttggcag atgatttcgc caattgcgga tagaacgcag cgtctggaaa
     6901 accgcgaata tcattgagat aggccacacc acgcgacaag gcataggctt gcgtcgcggg
     6961 ttgataactg tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc
     7021 gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg tcgggattgc tggatgccgg
     7081 accgaggtcg atcacatctg ccccctcggc catcagctta cgcgcctgcg caatggctgc
     7141 gtctggcgcc agataccggc ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc
     7201 gaaaatgatg agcgatttat tcatgggggc ttctataata ataataatcg agcatgagtc
     7261 tcatacggat gctcgggtcg aaagggaatc cccaggcgag taacctgttt gcggtgatcc
     7321 gtttcatgat gatgtatctg tagcattttg gaaataaata tatcatctct caataagtag
     7381 atatatttat cttttttgct tctttggtgc tgcaagtcac caatacgagg gaaacctgtt
     7441 acggagcggt ttaaccgtaa tcaacagtga ctggtgtcat gttcggaaga ttaattgata
     7501 gtaatctaca aaggatcttc ttgagatcct tttcaatgaa tcgtaatctc ttgctctgta
     7561 aacgaaaaaa ccgcctgggg aggcggtttg ttcgaaggtt aagtcagttg gggaactgct
     7621 taacccggta acagggttca cgagaccgcc attaccaaaa ctgttctttc agtgtagccg
     7681 cagtcaggcc tctacttcaa gacctcttaa tctatcttct cgtgcactcc tgttactaat
     7741 ggctgctgcc agtggcgttt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc
     7801 ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     7861 aactgccttc acggaactga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg
     7921 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg
     7981 aaacgcctgg gatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     8041 tttgcgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg tggccttatc
     8101 tcttctttgc taagtgtctt cctggtatct tccaggaaat ttccgtcccc gtccgtaagc
     8161 cggtaccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     8221 atatctcctg tatcgcatat tctgctgacg cgccggtgct gccttttttc tcctgccaca
     8281 tgaagcactt cactgatttt cgcaatcgtg gcaacatagt aagccagtat acactccgct
     8341 agcgctacat gactggttca gggctgcgcc ccgaaacccg ctaacaacct ctcagctact
     8401 ggatatcacc cc
//
