LOCUS       NZ_CP151316             8378 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R21.0552 plasmid pR21.0552_8k, complete
            sequence.
ACCESSION   NZ_CP151316
VERSION     NZ_CP151316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39735802
            Assembly: GCF_038364745.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038364745.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:43:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,428
            CDSs (total)                      :: 5,299
            Genes (coding)                    :: 4,560
            CDSs (with protein)               :: 4,560
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 739
            CDSs (without protein)            :: 739
            Pseudo Genes (ambiguous residues) :: 0 of 739
            Pseudo Genes (frameshifted)       :: 212 of 739
            Pseudo Genes (incomplete)         :: 527 of 739
            Pseudo Genes (internal stop)      :: 127 of 739
            Pseudo Genes (multiple problems)  :: 121 of 739
            Pseudo Genes (short protein)      :: 3 of 739
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R21.0552"
                     /db_xref="taxon:624"
                     /plasmid="pR21.0552_8k"
                     /collected_by="Taiwan CDC"
     gene            1..441
                     /locus_tag="AADX62_RS26970"
                     /old_locus_tag="AADX62_26960"
     CDS             1..441
                     /locus_tag="AADX62_RS26970"
                     /old_locus_tag="AADX62_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(500..991)
                     /locus_tag="AADX62_RS26975"
     CDS             complement(500..991)
                     /locus_tag="AADX62_RS26975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267191397.1"
                     /translation="MAGDLRRLRGDRDKKSVPHCIVHACMNGLFFLTTELHRSFSSYIT
                     YTSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAPATLHGHAINCVPVH
                     RFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVFGWFVACFTGFPSETP
                     "
                     /name="hypothetical protein"
     gene            complement(1197..1346)
                     /locus_tag="AADX62_RS26980"
                     /old_locus_tag="AADX62_26965"
     CDS             complement(1197..1346)
                     /locus_tag="AADX62_RS26980"
                     /old_locus_tag="AADX62_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1917..2075
                     /locus_tag="AADX62_RS26985"
                     /old_locus_tag="AADX62_26970"
     CDS             1917..2075
                     /locus_tag="AADX62_RS26985"
                     /old_locus_tag="AADX62_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2535..3350
                     /gene="sul2"
                     /locus_tag="AADX62_RS26990"
                     /old_locus_tag="AADX62_26975"
     ARG             2536..3347
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3411..4214
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX62_RS26995"
                     /old_locus_tag="AADX62_26980"
     ARG             3412..4211
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            4214..5050
                     /locus_tag="AADX62_RS27000"
                     /old_locus_tag="AADX62_26985"
     CDS             4214..5050
                     /locus_tag="AADX62_RS27000"
                     /old_locus_tag="AADX62_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5356..5586
                     /locus_tag="AADX62_RS27005"
                     /old_locus_tag="AADX62_26990"
     CDS             5356..5586
                     /locus_tag="AADX62_RS27005"
                     /old_locus_tag="AADX62_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="WP_256788757.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAERGREAWT
                     LGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(5618..6268)
                     /gene="tetR(A)"
                     /locus_tag="AADX62_RS27010"
                     /old_locus_tag="AADX62_26995"
     CDS             complement(5618..6268)
                     /gene="tetR(A)"
                     /locus_tag="AADX62_RS27010"
                     /old_locus_tag="AADX62_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            6374..7573
                     /gene="tet(A)"
                     /locus_tag="AADX62_RS27015"
                     /old_locus_tag="AADX62_27000"
     ARG             6375..7570
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7540..7728)
                     /locus_tag="AADX62_RS27020"
                     /old_locus_tag="AADX62_27005"
                     /pseudo=""
     CDS             complement(<7540..7728)
                     /locus_tag="AADX62_RS27020"
                     /old_locus_tag="AADX62_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7772..8161)
                     /locus_tag="AADX62_RS27025"
                     /old_locus_tag="AADX62_27010"
     CDS             complement(7772..8161)
                     /locus_tag="AADX62_RS27025"
                     /old_locus_tag="AADX62_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             4215..5047
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1382..1555)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1479..2084
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta
       61 tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag
      121 ttcgagacca gtgaactgat tcgggcctat ggcgagctgc gcacttctga gacaccaaaa
      181 aggcacaagg agggacactc caaatctgtc gttagccagg cggaaaatgc tcagctagac
      241 gtcattcagc agaaactgga cgctctgcag cagactgtta ccctaatgct tgaagacaag
      301 atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga gggaacaact acaggccgaa
      361 attagccgtc tggctgacgc gctggcacag gaaaaaaagc ggggattctg gtcgcggctg
      421 tttcgctccg gttcagagta acagtgcagg tgactctgat ttaccataac atctgttatc
      481 agcaccgcct gagaggccct cagggcgttt ctgacgggaa accggtaaaa caggcaacaa
      541 accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc
      601 gcacaagtgc atagcgcgga aattcacacc attcagccgg ggtaagtcct gtttccggcg
      661 ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg
      721 ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa gggggtgatg tacaaatggc
      781 acagaggatg aaagtgacgt atgagctgaa ggaatgatgt aaaagtgatg tataagtgat
      841 gtaaaagtga tgtataagtg atgtatgaac tgaaggagcg atgtaattct gtagtcagga
      901 agaataaccc gttcatgcag gcatgaacga tacagtgagg aacgcttttt ttgtcacgat
      961 ctccccttaa cctgcgtaaa tcacccgcca tggttgatta tttttcaacc tcaaaatggt
     1021 catacaccta acgcagccgc gattttcatc gcaactgcat gaattaaaag aaaaaaaagg
     1081 aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat tacatgaaaa
     1141 taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg aaaaagctat
     1201 aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt ctcttcagta
     1261 acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc
     1321 agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag gttgttagcg
     1381 ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat actggcttac
     1441 tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag
     1501 caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact
     1561 cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg ggacggaaat
     1621 ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg gcgaagccgt
     1681 ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca aatcagtggt
     1741 ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc
     1801 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc
     1861 tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc
     1921 acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa cttgagtcca
     1981 acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag
     2041 aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt
     2101 ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa
     2161 ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt
     2221 cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat
     2281 gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc tcgtattggt
     2341 gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag agatgatata
     2401 tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa acaggttact
     2461 cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta
     2521 ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg
     2581 acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta
     2641 agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg
     2701 ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca
     2761 aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct
     2821 tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc
     2881 cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg
     2941 cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg
     3001 cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg
     3061 gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg
     3121 atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc
     3181 tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc
     3241 ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg
     3301 acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc
     3361 gggatatttc tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa
     3421 ctaatatttt ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg
     3481 gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc
     3541 gccgcggtga gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg
     3601 cttgccccga ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg
     3661 caattccggg agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt
     3721 caatggggca gcaacttggc gctgttcaca gcctatcggt tgatcaatgt cagtttgagc
     3781 gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc
     3841 ccgacttctt accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg
     3901 aacgagagct accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg
     3961 atccctgcat gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg
     4021 accttgggcg gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg
     4081 ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg
     4141 tattggggat cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc
     4201 tgacttgggg ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc
     4261 tgttctcatt gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac
     4321 tcctgcaatc gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc
     4381 cgactatctg gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa
     4441 tctgatgttg ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg
     4501 cgactaccag gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga
     4561 ggaacccctg ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg
     4621 ggcgcgcgat gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc
     4681 cgatcaaatg atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga
     4741 aaacatcatg ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga
     4801 agtgggcttt ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct
     4861 cgatcctaga cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc
     4921 gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga
     4981 tggagaagag gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac
     5041 gtcatactag atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag
     5101 aatatcgctg gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gacccctgga
     5161 agccccgtag cgacgcggag aggggcgaga caagccaagg gcgcaggctc gatgcgcagc
     5221 acgacatagc cggttctcgc aaggacgaga atttccctgc ggtgcccctc aagtgtcaat
     5281 gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc cacgctagat ctatctcatc
     5341 tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac
     5401 gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag
     5461 aaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg
     5521 ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg
     5581 ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc
     5641 cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc
     5701 cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca
     5761 gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg
     5821 cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg
     5881 cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca
     5941 tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca
     6001 gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc
     6061 tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg
     6121 cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca
     6181 gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga
     6241 tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat
     6301 gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt
     6361 ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca ctgtcgcgct
     6421 cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt
     6481 tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttgatgca
     6541 atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt
     6601 gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg cgcctttcct
     6661 ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc
     6721 cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat
     6781 gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg
     6841 tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac
     6901 gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc
     6961 tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat
     7021 ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt
     7081 cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg
     7141 cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga
     7201 aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt
     7261 cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg
     7321 aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct
     7381 gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac
     7441 ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc
     7501 cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagaaatt cttcaaattc
     7561 ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg
     7621 gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg
     7681 accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct
     7741 tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg
     7801 aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccact cttacattgt
     7861 tcgcagtatc taggtacatg ttattgcgtg caccttcatg atgtataatg ttattaatga
     7921 cagcagaaag atcgacagaa ttaatattct tcgtacctgg gacagttatc gggtgtcggc
     7981 cagtgatgag accaaaggat gcagtataag atgaaccact actattatca tggccaccat
     8041 taccaccacc gttaaaacta cctcccgaac ttccagagcc gctatgacca tcattgtagg
     8101 ggtcatttgg tcccatgtga ccaaaaccac cgacagtacc gctatttaac tctgtactca
     8161 taaaattatc ctctttgact tttaaaaaca gcaaaaactg tatttataac aatgtgttta
     8221 tatacagtat tcgaaatact acgcccgtca aaaccacacc gtcaacaggt tttttgtgta
     8281 tttttcacgc ctcacgggca gcctcagacc ttttgtgtta aaacagtaat atgttctgct
     8341 gttacaatac ggtacacaca ggagacactg tcactttt
//
