LOCUS       NZ_CP151303             8401 bp    DNA     circular CON 18-APR-2024
DEFINITION  Shigella sonnei strain R22.1286 plasmid pR22.1286_8k, complete
            sequence.
ACCESSION   NZ_CP151303
VERSION     NZ_CP151303.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40122667
            Assembly: GCF_038363105.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     WGS of Shigella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Chiou,C.-S., Hong,Y.-P., Wang,Y.-W., Teng,R.-H. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038363105.1-RS_2024_04_18
            Annotation Date                   :: 04/18/2024 01:39:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 4,990
            Genes (coding)                    :: 4,358
            CDSs (with protein)               :: 4,358
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 632
            CDSs (without protein)            :: 632
            Pseudo Genes (ambiguous residues) :: 0 of 632
            Pseudo Genes (frameshifted)       :: 173 of 632
            Pseudo Genes (incomplete)         :: 436 of 632
            Pseudo Genes (internal stop)      :: 109 of 632
            Pseudo Genes (multiple problems)  :: 83 of 632
            Pseudo Genes (short protein)      :: 3 of 632
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151303.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R22.1286"
                     /db_xref="taxon:624"
                     /plasmid="pR22.1286_8k"
                     /collected_by="Taiwan CDC"
     gene            1..243
                     /locus_tag="AADX63_RS25400"
                     /old_locus_tag="AADX63_25385"
     CDS             1..243
                     /locus_tag="AADX63_RS25400"
                     /old_locus_tag="AADX63_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(275..925)
                     /gene="tetR(A)"
                     /locus_tag="AADX63_RS25405"
                     /old_locus_tag="AADX63_25390"
     CDS             complement(275..925)
                     /gene="tetR(A)"
                     /locus_tag="AADX63_RS25405"
                     /old_locus_tag="AADX63_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            1031..2230
                     /gene="tet(A)"
                     /locus_tag="AADX63_RS25410"
                     /old_locus_tag="AADX63_25395"
     ARG             1032..2227
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<2197..2385)
                     /locus_tag="AADX63_RS25415"
                     /old_locus_tag="AADX63_25400"
                     /pseudo=""
     CDS             complement(<2197..2385)
                     /locus_tag="AADX63_RS25415"
                     /old_locus_tag="AADX63_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2429..2818)
                     /locus_tag="AADX63_RS25420"
                     /old_locus_tag="AADX63_25405"
     CDS             complement(2429..2818)
                     /locus_tag="AADX63_RS25420"
                     /old_locus_tag="AADX63_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3036..3476
                     /locus_tag="AADX63_RS25425"
                     /old_locus_tag="AADX63_25410"
     CDS             3036..3476
                     /locus_tag="AADX63_RS25425"
                     /old_locus_tag="AADX63_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4243..4392)
                     /locus_tag="AADX63_RS25430"
                     /old_locus_tag="AADX63_25415"
     CDS             complement(4243..4392)
                     /locus_tag="AADX63_RS25430"
                     /old_locus_tag="AADX63_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4963..5121
                     /locus_tag="AADX63_RS25435"
                     /old_locus_tag="AADX63_25420"
     CDS             4963..5121
                     /locus_tag="AADX63_RS25435"
                     /old_locus_tag="AADX63_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5581..6396
                     /gene="sul2"
                     /locus_tag="AADX63_RS25440"
                     /old_locus_tag="AADX63_25425"
     ARG             5582..6393
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6457..7260
                     /gene="aph(3'')-Ib"
                     /locus_tag="AADX63_RS25445"
                     /old_locus_tag="AADX63_25430"
     ARG             6458..7257
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7260..8096
                     /locus_tag="AADX63_RS25450"
                     /old_locus_tag="AADX63_25435"
     CDS             7260..8096
                     /locus_tag="AADX63_RS25450"
                     /old_locus_tag="AADX63_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             7261..8093
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4428..4601)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4525..5130
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaagacgg ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg
       61 acccgcgcca cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag
      121 gccgagcgcg gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc
      181 tgctacgggc gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg
      241 tagtgagtgg gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt
      301 caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct
      361 gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg
      421 gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct
      481 gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt
      541 ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct
      601 gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg
      661 cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg
      721 gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc
      781 gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt
      841 tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca
      901 ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc
      961 caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg
     1021 gaggccagac gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga
     1081 cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca
     1141 ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt
     1201 tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct
     1261 cgtctcgctg gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg
     1321 ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg
     1381 cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag
     1441 cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt
     1501 ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg
     1561 ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct
     1621 caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc
     1681 ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg
     1741 cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat
     1801 tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag
     1861 gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc
     1921 gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat
     1981 gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca
     2041 aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc
     2101 gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct
     2161 ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agaaattctt caaattcccg
     2221 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
     2281 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
     2341 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
     2401 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
     2461 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccactctt acattgttcg
     2521 cagtatctag gtacatgtta ttgcgtgcac cttcatgatg tataatgtta ttaatgacag
     2581 cagaaagatc gacagaatta atattcttcg tacctgggac agttatcggg tgtcggccag
     2641 tgatgagacc aaaggatgca gtataagatg aaccactact attatcatgg ccaccattac
     2701 caccaccgtt aaaactacct cccgaacttc cagagccgct atgaccatca ttgtaggggt
     2761 catttggtcc catgtgacca aaaccaccga cagtaccgct atttaactct gtactcataa
     2821 aattatcctc tttgactttt aaaaacagca aaaactgtat ttataacaat gtgtttatat
     2881 acagtattcg aaatactacg cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt
     2941 ttcacgcctc acgggcagcc tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt
     3001 acaatacggt acacacagga gacactgtca cttttatggc atggcacaca gttcaagaga
     3061 cactgaaatt aacaggaaag tcgcgctcgc aattatatcg tgacatggct aaaggccttg
     3121 tcagctatcg cacaggaaaa aatgatcgcc gggagttcga gaccagtgaa ctgattcggg
     3181 cctatggcga gctgcgcact tctgagacac caaaaaggca caaggaggga cactccaaat
     3241 ctgtcgttag ccaggcggaa aatgctcagc tagacgtcat tcagcagaaa ctggacgctc
     3301 tgcagcagac tgttacccta atgcttgaag acaagatggc gcgggaagca gaaaaccgcc
     3361 tgcgaaatga ggagagggaa caactacagg ccgaaattag ccgtctggct gacgcgctgg
     3421 cacaggaaaa aaagcgggga ttctggtcgc ggctgtttcg ctccggttca gagtaacagt
     3481 gcaggtgact ctgatttacc ataacatctg ttatcagcac cgcctgagag gccctcaggg
     3541 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
     3601 cgcaggctct gtttttacgg catgcatgag tatgcgcaca agtgcatagc gcggaaattc
     3661 acaccattca gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac
     3721 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aacccgctgt
     3781 cagcacgttc aggaaggggg tgatgtacaa atggcacaga ggatgaaagt gacgtatgag
     3841 ctgaaggaat gatgtaaaag tgatgtataa gtgatgtata agtgatgtat aagtgatgta
     3901 aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     3961 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     4021 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     4081 agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa aaaaggaata tttcgttttc
     4141 acgagtaata cccgggtatt aaaaatccac tgtaattaca tgaaaataaa gaaataaaag
     4201 gtaataccgg agtattacca cgtacgcggg caagcgaaaa agctataacg cttcccggcc
     4261 gacaggcgac ccaaaatcca caggggcatg caggttctct tcagtaacct gcgccattaa
     4321 atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc gaatccagtt gttcatcata
     4381 ttcagatggc atggggtgat atccagtagc tgagaggttg ttagcgggtt tcggggcgca
     4441 gccctgaacc agtcatgtag cgctagcgga gtgtatactg gcttactatg ttgccacgat
     4501 tgcgaaaatc agtgaagtgc ttcatgtggc aggagaaaaa aggcagcacc ggcgcgtcag
     4561 cagaatatgc gatacaggag atattccgct tcctcgctca ctgactcgct acgctcggtc
     4621 gttcggctgc ggcgagcggt accggcttac ggacggggac ggaaatttcc tggaagatac
     4681 caggaagaca cttagcaaag aagagataag gccacggcga agccgttttt ccataggctc
     4741 cgcccccctg acaagcatcg caaaatctga cgctcaaatc agtggtggcg aaacccgaca
     4801 ggactataaa gatcccaggc gtttccccct ggtagctccc tcgtgcgctc tcctgttcct
     4861 gcctttcggt ttaccggtgt cattccgctg ttatggccgc ggtttctcat tccacgcctg
     4921 acactcagtt ccgtgaaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     4981 cagtccgacc gctgcgcctt gtccggtaac tatcaacttg agtccaaccc ggaaagacac
     5041 gacaaaacgc cactggcagc agccattagt aacaggagtg cacgagaaga tagattaaga
     5101 ggtcttgaag tagaggcctg actgcggcta cactgaaaga acagttttgg taatggcggt
     5161 ctcgtgaacc ctgttaccgg gttaagcagt tccccaactg acttaacctt cgaacaaacc
     5221 gcctccccag gcggtttttt cgtttacaga gcaagagatt acgattcatt gaaaaggatc
     5281 tcaagaagat cctttgtaga ttactatcaa ttaatcttcc gaacatgaca ccagtcactg
     5341 ttgattacgg ttaaaccgct ccgtaacagg tttccctcgt attggtgact tgcagcacca
     5401 aagaagcaaa aaagataaat atatctactt attgagagat gatatattta tttccaaaat
     5461 gctacagata catcatcatg aaacggatca ccgcaaacag gttactcgcc tggggattcc
     5521 ctttcgaccc gagcatccgt atgagactca tgctcgatta ttattattat agaagccccc
     5581 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
     5641 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
     5701 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
     5761 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
     5821 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
     5881 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
     5941 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
     6001 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
     6061 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
     6121 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
     6181 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
     6241 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
     6301 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
     6361 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
     6421 tattcgcgct tcatcagaaa actgaaggaa cctccattga atcgaactaa tatttttttt
     6481 ggtgaatcgc attctgactg gttgcctgtc agaggcggag aatctggtga ttttgttttt
     6541 cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc
     6601 gctggagagc gtgaccgcct catttggctc aaaggtcgag gtgtggcttg ccccgaggtc
     6661 atcaactggc aggaggaaca ggagggtgca tgcttggtga taacggcaat tccgggagta
     6721 ccggcggctg atctgtctgg agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa
     6781 cttggcgctg ttcacagcct atcggttgat caatgtccgt ttgagcgcag gctgtcgcga
     6841 atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg
     6901 gacgaggaca agagtacgcc gcagctcgat cttttggctc gtgtcgaacg agagctaccg
     6961 gtgcggctcg accaagagcg caccgatatg gttgtttgcc atggtgatcc ctgcatgccg
     7021 aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct tgggcggctc
     7081 ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga agagaactgg
     7141 gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt ggggatcgaa
     7201 gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac ttggggttga
     7261 tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc gcaacctgtt ctcattgcgg
     7321 acaccttttc cagcctcgtt tggaaagttt cattgccaga cgggactcct gcaatcgtca
     7381 agggattgaa acctatagaa gacattgctg atgaactgcg cggggccgac tatctggtat
     7441 ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga gaacaatctg atgttgctcg
     7501 aatatgccgg ggagcgaatg ctctctcaca tcgttgccga gcacggcgac taccaggcga
     7561 ccgaaattgc agcggaacta atggcgaagc tgtatgccgc atctgaggaa cccctgcctt
     7621 ctgcccttct cccgatccgg gatcgctttg cagctttgtt tcagcgggcg cgcgatgatc
     7681 aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat tatagccgat caaatgatga
     7741 gcaatgcctc ggaactgcgt gggctacatg gcgatctgca tcatgaaaac atcatgttct
     7801 ccagtcgcgg ctggctggtg atagatcccg tcggtctggt cggtgaagtg ggctttggcg
     7861 ccgccaatat gttctacgat ccggctgaca gagacgacct ttgtctcgat cctagacgca
     7921 ttgcacagat ggcggacgca ttctctcgtg cgctggacgt cgatccgcgt cgcctgctcg
     7981 accaggcgta cgcttatggg tgcctttccg cagcttggaa cgcggatgga gaagaggagc
     8041 aacgcgatct agctatcgcg gccgcgatca agcaggtgcg acagacgtca tactagatat
     8101 caagcgactt ctcctatccc ctgggaacac atcaatctca ccggagaata tcgctggcca
     8161 aagccttagc gtaggattcc gccccttccc gcaaacgacc cctggaagcc ccgtagcgac
     8221 gcggagaggg gcgagacaag ccaagggcgc aggctcgatg cgcagcacga catagccggt
     8281 tctcgcaagg acgagaattt ccctgcggtg cccctcaagt gtcaatgaaa gtttccaacg
     8341 cgagccattc gcgagagcct tgagtccacg ctagatctat ctcatctgcg caaggcagaa
     8401 c
//
