LOCUS       NZ_CP151201             1452 bp    DNA     circular CON 17-APR-2024
DEFINITION  Klebsiella variicola strain KVTMDU plasmid p14_KVTMDU, complete
            sequence.
ACCESSION   NZ_CP151201
VERSION     NZ_CP151201.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23446385
            Assembly: GCF_038178705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Ota,Y.
  TITLE     Emergence of KPC-2-producing Klebsiella variicola from hospital
            sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Ota,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. July-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.3x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038178705.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 11:53:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,118
            CDSs (total)                      :: 5,998
            Genes (coding)                    :: 5,805
            CDSs (with protein)               :: 5,805
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 68 of 193
            Pseudo Genes (incomplete)         :: 125 of 193
            Pseudo Genes (internal stop)      :: 32 of 193
            Pseudo Genes (multiple problems)  :: 29 of 193
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP151201.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /strain="KVTMDU"
                     /isolation_source="sewage"
                     /db_xref="taxon:244366"
                     /plasmid="p14_KVTMDU"
                     /geo_loc_name="Japan"
                     /lat_lon="35.7014 N 139.7642 E"
                     /collection_date="2021-06"
                     /collected_by="Tokyo Medical and Dental Hospital"
     gene            185..1054
                     /locus_tag="LQ252_RS30590"
                     /old_locus_tag="LQ252_30595"
     CDS             185..1054
                     /locus_tag="LQ252_RS30590"
                     /old_locus_tag="LQ252_30595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_341723213.1"
                     /translation="MLLNKDRFGKLTSKSLDSIAAIGLREADAELPCLFRNNSTEISSK
                     TPLEPLSPLAFELSTNLKKSASALAWNVQYFVDTYGLSNVGFLTLTFRDHVTDPKEAQR
                     RFNSLKTHILSNRYRAYIRVMEPMKSGRIHYHLLVALHSDIRTGFDFPAVYRQDYSSAN
                     KAIRSEWSFWRKTAPKYGFGRTELMPVRSNSEGIGRYVGKYISKGIESRTEQFKGVRLV
                     EYSRKAKIASTRFQFVSDGSYEWRRKLSIFVHYIADNMGCEPTFDGLRRVLGSRWSYHW
                     RDFIMNIE"
                     /name="hypothetical protein"
ORIGIN
        1 atgggttccg accactgact aatctgcaac accatgagtg acccccgttt gaccagttgg
       61 ccgaatcgct gtaacgttgc cccgatggcc aaaaagcaaa acccccgatg cgtcaacatc
      121 aggggtttat ggtcagtaag catttctacg gctcaatgtg tactttactc aactggacaa
      181 ctggatgtta ctcaacaaag accgctttgg caaactcacg tcaaaatccc tcgattctat
      241 tgctgctatc ggactccgcg aggcggacgc cgagcttcct tgtctattta gaaacaactc
      301 aaccgagatt tcttcaaaaa cacctctcga acccttatca cctctggctt ttgagctttc
      361 tacaaacctc aaaaaatctg cctcggctct ggcatggaac gtacaatatt ttgtcgatac
      421 ctacggcctt tcaaacgttg gatttttaac ccttaccttt cgtgaccatg tgaccgaccc
      481 taaagaggcg cagcgccgtt ttaattctct taaaacccat attttgagta atagataccg
      541 cgcttacatc cgtgtcatgg agcctatgaa gtcagggcgc atccactatc acttactcgt
      601 tgcactgcac tccgacattc gcaccggatt cgatttcccc gctgtctatc gccaggacta
      661 ctcctccgca aacaaggcta tccggtctga atggtcattc tggcgcaaga ctgcgcctaa
      721 atatggattc ggtcgcactg aattgatgcc cgttcggtca aattctgagg ggatagggcg
      781 ctatgtaggc aaatacattt ctaagggcat agaatcgcgt acagagcaat ttaagggcgt
      841 caggctggta gagtattccc gcaaggctaa aatcgcttct acgcgcttcc agttcgtttc
      901 tgacgggtct tatgagtggc gtcgcaaact ttctatcttc gttcactaca tcgcggataa
      961 catgggctgt gaaccaacct ttgacggctt acgccgtgtt cttggctccc gctggtcgta
     1021 tcactggcgt gattttatta tgaacatcga gtagtccacg ctgtttaata gtggactctt
     1081 gttccaaact ggaacaacac tcacaactaa ctcggcctat tcttttgatt tataaggatt
     1141 tttgtcattt tctgcttact ggttaaaaaa taagctgatt taacaaatat ttaacgcgaa
     1201 atttaacaaa acattaacgt ttacaattta aatataatag tggactcttt ttccatattg
     1261 gaacactttc cgcctcacgc agccagattg taaatctgtc agcgttcacc gttgctgaac
     1321 tcgtcaaatg ctaaatcggg ggtcccttta gggttccgat ttagtgtttg tacgacaccc
     1381 cgaataaata cacttcttgc tggtgtgtgt tacgtgcgca gcaggtgaca cacacctgta
     1441 agacgggttt ta
//
